Bismark report for: ERR12308514_val_1.fq.gz and ERR12308514_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 71554113 Number of paired-end alignments with a unique best hit: 18726905 Mapping efficiency: 26.2% Sequence pairs with no alignments under any condition: 39891908 Sequence pairs did not map uniquely: 12935300 Sequence pairs which were discarded because genomic sequence could not be extracted: 4 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 9352529 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 9374372 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 929305588 Total methylated C's in CpG context: 25722979 Total methylated C's in CHG context: 10486049 Total methylated C's in CHH context: 25976199 Total methylated C's in Unknown context: 330202 Total unmethylated C's in CpG context: 99775782 Total unmethylated C's in CHG context: 143975694 Total unmethylated C's in CHH context: 623368885 Total unmethylated C's in Unknown context: 5074770 C methylated in CpG context: 20.5% C methylated in CHG context: 6.8% C methylated in CHH context: 4.0% C methylated in Unknown context (CN or CHN): 6.1% Bismark completed in 0d 4h 1m 30s