SUMMARISING RUN PARAMETERS ========================== Input filename: ERR12308513_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 211937). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR12308513_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1276.429 s (15.630 µs/read; 3.84 M reads/minute). === Summary === Total reads processed: 81,667,318 Reads with adapters: 45,404,038 (55.6%) Reads written (passing filters): 81,667,318 (100.0%) Total basepairs processed: 12,331,765,018 bp Quality-trimmed: 42,306,107 bp (0.3%) Total written (filtered): 11,470,897,612 bp (93.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 45404038 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 33.5% C: 1.1% G: 16.4% T: 49.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 18599663 20416829.5 0 18599663 2 3132305 5104207.4 0 3132305 3 1538554 1276051.8 0 1538554 4 837751 319013.0 0 837751 5 437421 79753.2 0 437421 6 376045 19938.3 0 376045 7 373521 4984.6 0 373521 8 415929 1246.1 0 415929 9 365430 311.5 0 363985 1445 10 392092 77.9 1 359894 32198 11 421171 19.5 1 375376 45795 12 440643 4.9 1 397365 43278 13 380252 1.2 1 348617 31635 14 398990 1.2 1 354689 44301 15 408972 1.2 1 373479 35493 16 391077 1.2 1 347490 43587 17 436960 1.2 1 386225 50735 18 498419 1.2 1 460772 37647 19 237609 1.2 1 218973 18636 20 373104 1.2 1 343733 29371 21 404468 1.2 1 370213 34255 22 332336 1.2 1 307001 25335 23 397179 1.2 1 363946 33233 24 370496 1.2 1 328912 41584 25 374574 1.2 1 347896 26678 26 314913 1.2 1 293739 21174 27 380479 1.2 1 350606 29873 28 345324 1.2 1 322763 22561 29 356608 1.2 1 324015 32593 30 419725 1.2 1 394005 25720 31 218834 1.2 1 204895 13939 32 342729 1.2 1 321521 21208 33 305363 1.2 1 282150 23213 34 343188 1.2 1 316369 26819 35 294634 1.2 1 279291 15343 36 308411 1.2 1 285504 22907 37 301314 1.2 1 284541 16773 38 299312 1.2 1 276623 22689 39 339243 1.2 1 317416 21827 40 300995 1.2 1 286592 14403 41 234927 1.2 1 213943 20984 42 286930 1.2 1 271399 15531 43 349560 1.2 1 321465 28095 44 195139 1.2 1 182002 13137 45 418868 1.2 1 400960 17908 46 84350 1.2 1 77291 7059 47 243007 1.2 1 230378 12629 48 293636 1.2 1 277873 15763 49 194124 1.2 1 184474 9650 50 234971 1.2 1 220111 14860 51 236809 1.2 1 221466 15343 52 218655 1.2 1 207284 11371 53 209324 1.2 1 199050 10274 54 227941 1.2 1 213141 14800 55 284723 1.2 1 271437 13286 56 117779 1.2 1 111108 6671 57 236968 1.2 1 225910 11058 58 247917 1.2 1 238547 9370 59 99932 1.2 1 93598 6334 60 196989 1.2 1 190710 6279 61 119201 1.2 1 113615 5586 62 178891 1.2 1 170014 8877 63 207230 1.2 1 199757 7473 64 152832 1.2 1 147450 5382 65 88473 1.2 1 83724 4749 66 157108 1.2 1 150581 6527 67 168907 1.2 1 162095 6812 68 182515 1.2 1 175068 7447 69 155361 1.2 1 147634 7727 70 267936 1.2 1 257633 10303 71 18016 1.2 1 16450 1566 72 22178 1.2 1 20869 1309 73 66382 1.2 1 63924 2458 74 90295 1.2 1 87045 3250 75 96112 1.2 1 92396 3716 76 95902 1.2 1 92094 3808 77 91918 1.2 1 88332 3586 78 89273 1.2 1 85822 3451 79 85308 1.2 1 82099 3209 80 81978 1.2 1 78702 3276 81 77646 1.2 1 74538 3108 82 73834 1.2 1 71098 2736 83 70164 1.2 1 67503 2661 84 66989 1.2 1 64400 2589 85 63853 1.2 1 61490 2363 86 60237 1.2 1 57953 2284 87 56810 1.2 1 54610 2200 88 53568 1.2 1 51637 1931 89 50590 1.2 1 48767 1823 90 48273 1.2 1 46476 1797 91 45431 1.2 1 43759 1672 92 42616 1.2 1 41043 1573 93 40213 1.2 1 38681 1532 94 37285 1.2 1 35898 1387 95 35252 1.2 1 33985 1267 96 32581 1.2 1 31447 1134 97 30205 1.2 1 29151 1054 98 28272 1.2 1 27241 1031 99 26589 1.2 1 25670 919 100 24627 1.2 1 23709 918 101 22591 1.2 1 21831 760 102 20966 1.2 1 20256 710 103 19189 1.2 1 18578 611 104 17301 1.2 1 16685 616 105 15927 1.2 1 15398 529 106 14028 1.2 1 13569 459 107 12935 1.2 1 12508 427 108 11311 1.2 1 10919 392 109 10130 1.2 1 9792 338 110 8885 1.2 1 8594 291 111 7948 1.2 1 7666 282 112 6627 1.2 1 6387 240 113 5740 1.2 1 5532 208 114 4713 1.2 1 4551 162 115 3842 1.2 1 3716 126 116 3193 1.2 1 3077 116 117 2682 1.2 1 2582 100 118 2039 1.2 1 1961 78 119 1535 1.2 1 1466 69 120 1395 1.2 1 1331 64 121 1077 1.2 1 1034 43 122 769 1.2 1 735 34 123 542 1.2 1 510 32 124 385 1.2 1 360 25 125 323 1.2 1 282 41 126 247 1.2 1 218 29 127 204 1.2 1 177 27 128 169 1.2 1 151 18 129 146 1.2 1 123 23 130 110 1.2 1 100 10 131 123 1.2 1 110 13 132 100 1.2 1 83 17 133 101 1.2 1 90 11 134 65 1.2 1 56 9 135 116 1.2 1 93 23 136 65 1.2 1 48 17 137 75 1.2 1 60 15 138 45 1.2 1 34 11 139 69 1.2 1 49 20 140 46 1.2 1 34 12 141 61 1.2 1 47 14 142 42 1.2 1 28 14 143 32 1.2 1 27 5 144 61 1.2 1 38 23 145 41 1.2 1 23 18 146 25 1.2 1 10 15 147 44 1.2 1 24 20 148 41 1.2 1 22 19 149 43 1.2 1 20 23 150 66 1.2 1 14 52 151 6395 1.2 1 16 6379 RUN STATISTICS FOR INPUT FILE: ERR12308513_1.fastq.gz ============================================= 81667318 sequences processed in total