Bismark report for: ERR12308512_val_1.fq.gz and ERR12308512_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 80547105 Number of paired-end alignments with a unique best hit: 31806719 Mapping efficiency: 39.5% Sequence pairs with no alignments under any condition: 35268146 Sequence pairs did not map uniquely: 13472240 Sequence pairs which were discarded because genomic sequence could not be extracted: 15 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 15901415 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 15905289 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1801118103 Total methylated C's in CpG context: 78833532 Total methylated C's in CHG context: 27116974 Total methylated C's in CHH context: 65419620 Total methylated C's in Unknown context: 450851 Total unmethylated C's in CpG context: 195932731 Total unmethylated C's in CHG context: 321332166 Total unmethylated C's in CHH context: 1112483080 Total unmethylated C's in Unknown context: 6746046 C methylated in CpG context: 28.7% C methylated in CHG context: 7.8% C methylated in CHH context: 5.6% C methylated in Unknown context (CN or CHN): 6.3% Bismark completed in 0d 4h 35m 31s