SUMMARISING RUN PARAMETERS ========================== Input filename: ERR12308512_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 150927). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR12308512_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1285.648 s (15.952 µs/read; 3.76 M reads/minute). === Summary === Total reads processed: 80,593,414 Reads with adapters: 40,506,447 (50.3%) Reads written (passing filters): 80,593,414 (100.0%) Total basepairs processed: 12,169,605,514 bp Quality-trimmed: 42,020,803 bp (0.3%) Total written (filtered): 11,570,326,998 bp (95.1%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 40506447 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 33.4% C: 2.7% G: 18.4% T: 45.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 18916562 20148353.5 0 18916562 2 3610298 5037088.4 0 3610298 3 1656803 1259272.1 0 1656803 4 808115 314818.0 0 808115 5 384227 78704.5 0 384227 6 330845 19676.1 0 330845 7 326765 4919.0 0 326765 8 357707 1229.8 0 357707 9 307192 307.4 0 305570 1622 10 329876 76.9 1 304497 25379 11 350702 19.2 1 313593 37109 12 362475 4.8 1 329418 33057 13 319319 1.2 1 293966 25353 14 335342 1.2 1 300092 35250 15 341976 1.2 1 313923 28053 16 321650 1.2 1 286663 34987 17 364856 1.2 1 323678 41178 18 403499 1.2 1 375494 28005 19 195228 1.2 1 181037 14191 20 297817 1.2 1 274978 22839 21 309361 1.2 1 285063 24298 22 261398 1.2 1 242112 19286 23 315793 1.2 1 290826 24967 24 279306 1.2 1 247282 32024 25 292757 1.2 1 272367 20390 26 241658 1.2 1 226608 15050 27 290960 1.2 1 268760 22200 28 274623 1.2 1 256411 18212 29 278186 1.2 1 251787 26399 30 307762 1.2 1 288994 18768 31 169377 1.2 1 158436 10941 32 269130 1.2 1 253133 15997 33 200978 1.2 1 184496 16482 34 267902 1.2 1 248085 19817 35 182057 1.2 1 171673 10384 36 228756 1.2 1 214702 14054 37 270827 1.2 1 247311 23516 38 177846 1.2 1 167020 10826 39 219066 1.2 1 207010 12056 40 183400 1.2 1 173638 9762 41 213655 1.2 1 198220 15435 42 208878 1.2 1 196555 12323 43 249928 1.2 1 231957 17971 44 108191 1.2 1 100208 7983 45 291591 1.2 1 278734 12857 46 53398 1.2 1 48689 4709 47 167501 1.2 1 158285 9216 48 238008 1.2 1 224698 13310 49 111597 1.2 1 105619 5978 50 164957 1.2 1 154249 10708 51 155780 1.2 1 145671 10109 52 144812 1.2 1 137636 7176 53 134242 1.2 1 127737 6505 54 177401 1.2 1 166453 10948 55 201167 1.2 1 193107 8060 56 60563 1.2 1 56340 4223 57 191084 1.2 1 184152 6932 58 128007 1.2 1 123383 4624 59 68133 1.2 1 64295 3838 60 122081 1.2 1 118773 3308 61 50217 1.2 1 47267 2950 62 121499 1.2 1 115627 5872 63 155894 1.2 1 151589 4305 64 64938 1.2 1 62543 2395 65 48322 1.2 1 45527 2795 66 104543 1.2 1 100257 4286 67 94806 1.2 1 90996 3810 68 105128 1.2 1 100752 4376 69 93491 1.2 1 88822 4669 70 152976 1.2 1 146988 5988 71 12116 1.2 1 10977 1139 72 13041 1.2 1 12184 857 73 36918 1.2 1 35352 1566 74 50481 1.2 1 48431 2050 75 53985 1.2 1 51817 2168 76 54062 1.2 1 51708 2354 77 51916 1.2 1 49652 2264 78 48843 1.2 1 46866 1977 79 46290 1.2 1 44316 1974 80 43893 1.2 1 41952 1941 81 41408 1.2 1 39608 1800 82 38700 1.2 1 37050 1650 83 36005 1.2 1 34478 1527 84 33746 1.2 1 32287 1459 85 31850 1.2 1 30528 1322 86 29156 1.2 1 27978 1178 87 27978 1.2 1 26782 1196 88 25843 1.2 1 24762 1081 89 23744 1.2 1 22719 1025 90 22341 1.2 1 21377 964 91 20664 1.2 1 19789 875 92 18986 1.2 1 18168 818 93 17621 1.2 1 16918 703 94 16450 1.2 1 15729 721 95 15169 1.2 1 14539 630 96 14103 1.2 1 13525 578 97 13074 1.2 1 12512 562 98 11760 1.2 1 11292 468 99 11029 1.2 1 10534 495 100 10459 1.2 1 9990 469 101 9495 1.2 1 9110 385 102 8610 1.2 1 8236 374 103 8040 1.2 1 7694 346 104 7238 1.2 1 6915 323 105 6533 1.2 1 6249 284 106 5858 1.2 1 5588 270 107 5417 1.2 1 5202 215 108 4801 1.2 1 4569 232 109 4335 1.2 1 4151 184 110 3872 1.2 1 3669 203 111 3498 1.2 1 3329 169 112 3168 1.2 1 3021 147 113 2723 1.2 1 2562 161 114 2301 1.2 1 2179 122 115 2102 1.2 1 1967 135 116 1726 1.2 1 1636 90 117 1565 1.2 1 1465 100 118 1363 1.2 1 1257 106 119 1153 1.2 1 1062 91 120 1179 1.2 1 1097 82 121 1044 1.2 1 961 83 122 653 1.2 1 599 54 123 594 1.2 1 515 79 124 437 1.2 1 386 51 125 376 1.2 1 313 63 126 291 1.2 1 250 41 127 272 1.2 1 231 41 128 213 1.2 1 170 43 129 165 1.2 1 136 29 130 151 1.2 1 117 34 131 131 1.2 1 106 25 132 106 1.2 1 84 22 133 131 1.2 1 97 34 134 81 1.2 1 58 23 135 99 1.2 1 81 18 136 73 1.2 1 51 22 137 86 1.2 1 66 20 138 72 1.2 1 48 24 139 74 1.2 1 52 22 140 49 1.2 1 33 16 141 74 1.2 1 45 29 142 53 1.2 1 19 34 143 42 1.2 1 24 18 144 62 1.2 1 24 38 145 53 1.2 1 32 21 146 45 1.2 1 19 26 147 47 1.2 1 18 29 148 68 1.2 1 19 49 149 66 1.2 1 35 31 150 97 1.2 1 24 73 151 18949 1.2 1 46 18903 RUN STATISTICS FOR INPUT FILE: ERR12308512_1.fastq.gz ============================================= 80593414 sequences processed in total