SUMMARISING RUN PARAMETERS ========================== Input filename: ERR12308511_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 127487). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR12308511_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1275.299 s (15.912 µs/read; 3.77 M reads/minute). === Summary === Total reads processed: 80,147,947 Reads with adapters: 39,124,260 (48.8%) Reads written (passing filters): 80,147,947 (100.0%) Total basepairs processed: 12,102,339,997 bp Quality-trimmed: 38,535,242 bp (0.3%) Total written (filtered): 11,587,843,618 bp (95.7%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 39124260 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 34.1% C: 2.2% G: 17.5% T: 46.1% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 19830656 20036986.8 0 19830656 2 3634238 5009246.7 0 3634238 3 1630966 1252311.7 0 1630966 4 793671 313077.9 0 793671 5 346266 78269.5 0 346266 6 297576 19567.4 0 297576 7 294116 4891.8 0 294116 8 318765 1223.0 0 318765 9 275830 305.7 0 274426 1404 10 295602 76.4 1 272284 23318 11 312841 19.1 1 279621 33220 12 321311 4.8 1 291326 29985 13 276864 1.2 1 254141 22723 14 298491 1.2 1 266726 31765 15 298158 1.2 1 273408 24750 16 286353 1.2 1 254598 31755 17 316096 1.2 1 280401 35695 18 349096 1.2 1 323209 25887 19 173442 1.2 1 160576 12866 20 257088 1.2 1 236713 20375 21 266798 1.2 1 244734 22064 22 228012 1.2 1 210452 17560 23 268613 1.2 1 246328 22285 24 242096 1.2 1 214529 27567 25 249263 1.2 1 230870 18393 26 207006 1.2 1 193510 13496 27 248648 1.2 1 229137 19511 28 236641 1.2 1 220291 16350 29 224361 1.2 1 203658 20703 30 263233 1.2 1 246500 16733 31 139127 1.2 1 130382 8745 32 208694 1.2 1 195085 13609 33 190395 1.2 1 174602 15793 34 210793 1.2 1 192522 18271 35 230222 1.2 1 216163 14059 36 125802 1.2 1 117181 8621 37 190231 1.2 1 178008 12223 38 180772 1.2 1 169772 11000 39 172531 1.2 1 159087 13444 40 172960 1.2 1 161433 11527 41 188525 1.2 1 175760 12765 42 167843 1.2 1 159276 8567 43 137942 1.2 1 125057 12885 44 135098 1.2 1 125298 9800 45 232136 1.2 1 221215 10921 46 52486 1.2 1 47765 4721 47 133710 1.2 1 126203 7507 48 170861 1.2 1 160362 10499 49 109667 1.2 1 103784 5883 50 126853 1.2 1 118596 8257 51 127981 1.2 1 118984 8997 52 121319 1.2 1 114355 6964 53 111204 1.2 1 105297 5907 54 122481 1.2 1 113959 8522 55 155786 1.2 1 147924 7862 56 61769 1.2 1 58033 3736 57 129204 1.2 1 122893 6311 58 126723 1.2 1 121393 5330 59 57058 1.2 1 53327 3731 60 102197 1.2 1 98736 3461 61 56518 1.2 1 53595 2923 62 95565 1.2 1 90250 5315 63 110407 1.2 1 106177 4230 64 71431 1.2 1 68651 2780 65 43522 1.2 1 40959 2563 66 82527 1.2 1 78711 3816 67 82431 1.2 1 78715 3716 68 87433 1.2 1 83670 3763 69 75127 1.2 1 70992 4135 70 126083 1.2 1 120589 5494 71 9648 1.2 1 8744 904 72 12044 1.2 1 11197 847 73 32773 1.2 1 31298 1475 74 43305 1.2 1 41423 1882 75 45559 1.2 1 43589 1970 76 45001 1.2 1 42905 2096 77 43572 1.2 1 41614 1958 78 41108 1.2 1 39173 1935 79 39298 1.2 1 37551 1747 80 37525 1.2 1 35740 1785 81 35204 1.2 1 33561 1643 82 33456 1.2 1 31932 1524 83 31092 1.2 1 29666 1426 84 29360 1.2 1 28004 1356 85 27543 1.2 1 26306 1237 86 25628 1.2 1 24468 1160 87 24194 1.2 1 23025 1169 88 22565 1.2 1 21533 1032 89 21139 1.2 1 20230 909 90 19670 1.2 1 18750 920 91 18331 1.2 1 17513 818 92 17201 1.2 1 16426 775 93 16144 1.2 1 15429 715 94 14897 1.2 1 14208 689 95 13951 1.2 1 13322 629 96 12475 1.2 1 11935 540 97 11946 1.2 1 11408 538 98 11161 1.2 1 10664 497 99 10390 1.2 1 9962 428 100 9226 1.2 1 8832 394 101 8732 1.2 1 8344 388 102 8169 1.2 1 7811 358 103 7409 1.2 1 7041 368 104 6542 1.2 1 6245 297 105 6017 1.2 1 5732 285 106 5359 1.2 1 5144 215 107 4978 1.2 1 4737 241 108 4465 1.2 1 4246 219 109 4030 1.2 1 3838 192 110 3642 1.2 1 3478 164 111 3185 1.2 1 3012 173 112 2863 1.2 1 2693 170 113 2496 1.2 1 2364 132 114 2166 1.2 1 2050 116 115 1884 1.2 1 1776 108 116 1581 1.2 1 1487 94 117 1421 1.2 1 1305 116 118 1225 1.2 1 1143 82 119 997 1.2 1 925 72 120 1028 1.2 1 981 47 121 919 1.2 1 858 61 122 623 1.2 1 566 57 123 516 1.2 1 458 58 124 361 1.2 1 322 39 125 374 1.2 1 323 51 126 331 1.2 1 290 41 127 237 1.2 1 193 44 128 207 1.2 1 184 23 129 157 1.2 1 140 17 130 175 1.2 1 144 31 131 139 1.2 1 120 19 132 105 1.2 1 86 19 133 125 1.2 1 106 19 134 99 1.2 1 75 24 135 126 1.2 1 105 21 136 75 1.2 1 65 10 137 100 1.2 1 83 17 138 72 1.2 1 48 24 139 71 1.2 1 52 19 140 61 1.2 1 49 12 141 83 1.2 1 72 11 142 56 1.2 1 28 28 143 39 1.2 1 22 17 144 53 1.2 1 24 29 145 60 1.2 1 37 23 146 48 1.2 1 26 22 147 62 1.2 1 37 25 148 62 1.2 1 41 21 149 83 1.2 1 48 35 150 100 1.2 1 29 71 151 19635 1.2 1 70 19565 RUN STATISTICS FOR INPUT FILE: ERR12308511_1.fastq.gz ============================================= 80147947 sequences processed in total