SUMMARISING RUN PARAMETERS ========================== Input filename: ERR12308507_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 120118). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR12308507_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 1669.951 s (20.651 µs/read; 2.91 M reads/minute). === Summary === Total reads processed: 80,866,589 Reads with adapters: 39,237,829 (48.5%) Reads written (passing filters): 80,866,589 (100.0%) Total basepairs processed: 12,210,854,939 bp Quality-trimmed: 33,374,638 bp (0.3%) Total written (filtered): 11,720,834,122 bp (96.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 39237829 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 35.2% C: 1.4% G: 16.2% T: 47.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 21118447 20216647.2 0 21118447 2 3549922 5054161.8 0 3549922 3 1579210 1263540.5 0 1579210 4 762529 315885.1 0 762529 5 293543 78971.3 0 293543 6 251148 19742.8 0 251148 7 249188 4935.7 0 249188 8 273697 1233.9 0 273697 9 236890 308.5 0 235289 1601 10 254504 77.1 1 233319 21185 11 270701 19.3 1 241016 29685 12 280231 4.8 1 252985 27246 13 241527 1.2 1 221273 20254 14 254907 1.2 1 226740 28167 15 260671 1.2 1 238107 22564 16 241150 1.2 1 214175 26975 17 271789 1.2 1 240277 31512 18 301992 1.2 1 279002 22990 19 156292 1.2 1 144194 12098 20 226934 1.2 1 208767 18167 21 243176 1.2 1 222430 20746 22 203742 1.2 1 187993 15749 23 238812 1.2 1 218573 20239 24 223844 1.2 1 198649 25195 25 223105 1.2 1 207065 16040 26 190498 1.2 1 177675 12823 27 224861 1.2 1 207182 17679 28 207983 1.2 1 194222 13761 29 208169 1.2 1 188831 19338 30 249265 1.2 1 233523 15742 31 125093 1.2 1 117075 8018 32 195982 1.2 1 183472 12510 33 179353 1.2 1 164888 14465 34 214027 1.2 1 198103 15924 35 143479 1.2 1 135167 8312 36 182529 1.2 1 168602 13927 37 191614 1.2 1 179664 11950 38 157396 1.2 1 147153 10243 39 182921 1.2 1 169172 13749 40 181790 1.2 1 170532 11258 41 121522 1.2 1 113065 8457 42 154652 1.2 1 146352 8300 43 198031 1.2 1 183033 14998 44 111606 1.2 1 103497 8109 45 228596 1.2 1 219174 9422 46 47561 1.2 1 43294 4267 47 132472 1.2 1 125429 7043 48 159379 1.2 1 150336 9043 49 109962 1.2 1 104236 5726 50 126615 1.2 1 118423 8192 51 127485 1.2 1 118890 8595 52 119497 1.2 1 113132 6365 53 112119 1.2 1 106378 5741 54 121938 1.2 1 113794 8144 55 153393 1.2 1 146093 7300 56 64154 1.2 1 60213 3941 57 126275 1.2 1 120254 6021 58 129509 1.2 1 124492 5017 59 56649 1.2 1 52990 3659 60 100274 1.2 1 97059 3215 61 63017 1.2 1 59886 3131 62 95906 1.2 1 90846 5060 63 108174 1.2 1 104201 3973 64 76229 1.2 1 73369 2860 65 45509 1.2 1 42838 2671 66 81411 1.2 1 77849 3562 67 85493 1.2 1 81788 3705 68 91609 1.2 1 87832 3777 69 78393 1.2 1 74126 4267 70 127694 1.2 1 122656 5038 71 9158 1.2 1 8331 827 72 12031 1.2 1 11252 779 73 34060 1.2 1 32687 1373 74 44558 1.2 1 42773 1785 75 46805 1.2 1 44894 1911 76 46298 1.2 1 44335 1963 77 44412 1.2 1 42587 1825 78 42661 1.2 1 40927 1734 79 39956 1.2 1 38234 1722 80 38156 1.2 1 36522 1634 81 36306 1.2 1 34758 1548 82 33981 1.2 1 32515 1466 83 31755 1.2 1 30438 1317 84 30223 1.2 1 28963 1260 85 27834 1.2 1 26678 1156 86 26429 1.2 1 25353 1076 87 24698 1.2 1 23621 1077 88 23265 1.2 1 22317 948 89 21620 1.2 1 20784 836 90 20040 1.2 1 19259 781 91 18587 1.2 1 17790 797 92 17298 1.2 1 16585 713 93 15945 1.2 1 15305 640 94 14752 1.2 1 14135 617 95 13362 1.2 1 12820 542 96 12555 1.2 1 12028 527 97 11354 1.2 1 10876 478 98 10581 1.2 1 10132 449 99 9565 1.2 1 9156 409 100 8695 1.2 1 8346 349 101 7910 1.2 1 7620 290 102 7180 1.2 1 6876 304 103 6335 1.2 1 6113 222 104 5958 1.2 1 5700 258 105 5153 1.2 1 4940 213 106 4532 1.2 1 4323 209 107 4121 1.2 1 3938 183 108 3568 1.2 1 3420 148 109 3133 1.2 1 2994 139 110 2664 1.2 1 2562 102 111 2481 1.2 1 2390 91 112 2032 1.2 1 1929 103 113 1746 1.2 1 1653 93 114 1496 1.2 1 1412 84 115 1188 1.2 1 1128 60 116 1022 1.2 1 958 64 117 914 1.2 1 862 52 118 731 1.2 1 686 45 119 620 1.2 1 572 48 120 529 1.2 1 480 49 121 476 1.2 1 440 36 122 373 1.2 1 334 39 123 310 1.2 1 265 45 124 285 1.2 1 255 30 125 225 1.2 1 198 27 126 217 1.2 1 193 24 127 161 1.2 1 135 26 128 155 1.2 1 126 29 129 152 1.2 1 132 20 130 149 1.2 1 124 25 131 101 1.2 1 85 16 132 112 1.2 1 81 31 133 112 1.2 1 96 16 134 81 1.2 1 60 21 135 73 1.2 1 61 12 136 50 1.2 1 37 13 137 64 1.2 1 54 10 138 72 1.2 1 49 23 139 90 1.2 1 74 16 140 69 1.2 1 49 20 141 79 1.2 1 61 18 142 61 1.2 1 41 20 143 66 1.2 1 37 29 144 74 1.2 1 45 29 145 64 1.2 1 45 19 146 49 1.2 1 24 25 147 49 1.2 1 26 23 148 60 1.2 1 31 29 149 59 1.2 1 38 21 150 62 1.2 1 25 37 151 5591 1.2 1 48 5543 RUN STATISTICS FOR INPUT FILE: ERR12308507_2.fastq.gz ============================================= 80866589 sequences processed in total