SUMMARISING RUN PARAMETERS ========================== Input filename: ERR12308506_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 118336). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR12308506_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 1596.041 s (17.473 µs/read; 3.43 M reads/minute). === Summary === Total reads processed: 91,343,928 Reads with adapters: 43,541,493 (47.7%) Reads written (passing filters): 91,343,928 (100.0%) Total basepairs processed: 13,792,933,128 bp Quality-trimmed: 44,241,923 bp (0.3%) Total written (filtered): 13,264,013,460 bp (96.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 43541493 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 33.0% C: 3.5% G: 18.7% T: 44.8% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 21795393 22835982.0 0 21795393 2 4299104 5708995.5 0 4299104 3 1933798 1427248.9 0 1933798 4 945803 356812.2 0 945803 5 450046 89203.1 0 450046 6 382141 22300.8 0 382141 7 377941 5575.2 0 377941 8 406255 1393.8 0 406255 9 344247 348.4 0 342501 1746 10 368357 87.1 1 339690 28667 11 388742 21.8 1 348070 40672 12 403003 5.4 1 365859 37144 13 351194 1.4 1 322621 28573 14 369722 1.4 1 331305 38417 15 371627 1.4 1 341457 30170 16 348826 1.4 1 311460 37366 17 388374 1.4 1 345832 42542 18 432401 1.4 1 401152 31249 19 204122 1.4 1 189285 14837 20 313139 1.4 1 288271 24868 21 301265 1.4 1 277923 23342 22 274544 1.4 1 252577 21967 23 318320 1.4 1 293222 25098 24 261606 1.4 1 233436 28170 25 300426 1.4 1 278150 22276 26 232162 1.4 1 217770 14392 27 283918 1.4 1 261284 22634 28 307982 1.4 1 285978 22004 29 227441 1.4 1 207577 19864 30 286295 1.4 1 268471 17824 31 158496 1.4 1 148416 10080 32 231868 1.4 1 216614 15254 33 194571 1.4 1 180013 14558 34 215096 1.4 1 199608 15488 35 212178 1.4 1 192690 19488 36 206647 1.4 1 194155 12492 37 181711 1.4 1 170844 10867 38 189943 1.4 1 177299 12644 39 184933 1.4 1 171080 13853 40 189545 1.4 1 174876 14669 41 194615 1.4 1 180580 14035 42 164001 1.4 1 155941 8060 43 156641 1.4 1 141871 14770 44 126397 1.4 1 117196 9201 45 229559 1.4 1 218427 11132 46 54257 1.4 1 49170 5087 47 135220 1.4 1 126903 8317 48 189533 1.4 1 177601 11932 49 97966 1.4 1 92210 5756 50 124600 1.4 1 116058 8542 51 123377 1.4 1 114877 8500 52 114956 1.4 1 108486 6470 53 102085 1.4 1 96820 5265 54 133582 1.4 1 124859 8723 55 149903 1.4 1 143204 6699 56 48252 1.4 1 44737 3515 57 139699 1.4 1 134039 5660 58 91277 1.4 1 87520 3757 59 54462 1.4 1 51155 3307 60 86372 1.4 1 83750 2622 61 37672 1.4 1 35282 2390 62 88627 1.4 1 83835 4792 63 110028 1.4 1 106334 3694 64 44288 1.4 1 42304 1984 65 35648 1.4 1 33524 2124 66 76128 1.4 1 72543 3585 67 63061 1.4 1 60279 2782 68 67575 1.4 1 64563 3012 69 61583 1.4 1 58284 3299 70 106446 1.4 1 102212 4234 71 9841 1.4 1 8892 949 72 9524 1.4 1 8813 711 73 24407 1.4 1 23201 1206 74 33528 1.4 1 31955 1573 75 36017 1.4 1 34342 1675 76 35245 1.4 1 33625 1620 77 33879 1.4 1 32311 1568 78 32664 1.4 1 31165 1499 79 30614 1.4 1 29202 1412 80 29474 1.4 1 28064 1410 81 27294 1.4 1 26050 1244 82 25803 1.4 1 24603 1200 83 23717 1.4 1 22617 1100 84 22509 1.4 1 21443 1066 85 20874 1.4 1 19914 960 86 19466 1.4 1 18492 974 87 18571 1.4 1 17663 908 88 16938 1.4 1 16153 785 89 16216 1.4 1 15466 750 90 14609 1.4 1 13921 688 91 13786 1.4 1 13138 648 92 12919 1.4 1 12265 654 93 12058 1.4 1 11455 603 94 11083 1.4 1 10534 549 95 10576 1.4 1 10066 510 96 9568 1.4 1 9131 437 97 9092 1.4 1 8664 428 98 8495 1.4 1 8091 404 99 7470 1.4 1 7089 381 100 7267 1.4 1 6924 343 101 6636 1.4 1 6313 323 102 6229 1.4 1 5928 301 103 5584 1.4 1 5332 252 104 5116 1.4 1 4856 260 105 4708 1.4 1 4486 222 106 4181 1.4 1 3966 215 107 3891 1.4 1 3670 221 108 3644 1.4 1 3431 213 109 3296 1.4 1 3116 180 110 2891 1.4 1 2717 174 111 2714 1.4 1 2569 145 112 2471 1.4 1 2293 178 113 2209 1.4 1 2084 125 114 1871 1.4 1 1747 124 115 1799 1.4 1 1686 113 116 1498 1.4 1 1396 102 117 1323 1.4 1 1230 93 118 1194 1.4 1 1109 85 119 1093 1.4 1 1006 87 120 1674 1.4 1 1577 97 121 1309 1.4 1 1233 76 122 720 1.4 1 663 57 123 599 1.4 1 538 61 124 484 1.4 1 418 66 125 418 1.4 1 373 45 126 323 1.4 1 268 55 127 296 1.4 1 225 71 128 258 1.4 1 215 43 129 239 1.4 1 194 45 130 175 1.4 1 149 26 131 170 1.4 1 147 23 132 150 1.4 1 124 26 133 160 1.4 1 139 21 134 122 1.4 1 92 30 135 110 1.4 1 85 25 136 95 1.4 1 77 18 137 116 1.4 1 94 22 138 66 1.4 1 50 16 139 85 1.4 1 66 19 140 66 1.4 1 47 19 141 102 1.4 1 76 26 142 62 1.4 1 32 30 143 68 1.4 1 41 27 144 80 1.4 1 42 38 145 82 1.4 1 62 20 146 79 1.4 1 46 33 147 79 1.4 1 47 32 148 79 1.4 1 47 32 149 81 1.4 1 56 25 150 200 1.4 1 32 168 151 46102 1.4 1 108 45994 RUN STATISTICS FOR INPUT FILE: ERR12308506_2.fastq.gz ============================================= 91343928 sequences processed in total