SUMMARISING RUN PARAMETERS ========================== Input filename: ERR12308505_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 137361). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR12308505_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 1090.354 s (17.770 µs/read; 3.38 M reads/minute). === Summary === Total reads processed: 61,359,468 Reads with adapters: 30,432,201 (49.6%) Reads written (passing filters): 61,359,468 (100.0%) Total basepairs processed: 9,265,279,668 bp Quality-trimmed: 32,705,398 bp (0.4%) Total written (filtered): 8,850,801,476 bp (95.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 30432201 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 34.5% C: 2.3% G: 16.9% T: 46.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 15244745 15339867.0 0 15244745 2 2719388 3834966.8 0 2719388 3 1225883 958741.7 0 1225883 4 595493 239685.4 0 595493 5 268374 59921.4 0 268374 6 232764 14980.3 0 232764 7 230916 3745.1 0 230916 8 250952 936.3 0 250952 9 215403 234.1 0 214258 1145 10 232565 58.5 1 214922 17643 11 245179 14.6 1 220578 24601 12 254618 3.7 1 231579 23039 13 218019 0.9 1 201114 16905 14 233810 0.9 1 210231 23579 15 235187 0.9 1 216909 18278 16 223284 0.9 1 199936 23348 17 249514 0.9 1 222844 26670 18 287745 0.9 1 267914 19831 19 130414 0.9 1 121059 9355 20 211927 0.9 1 195980 15947 21 209851 0.9 1 194005 15846 22 184610 0.9 1 171287 13323 23 216278 0.9 1 199761 16517 24 191591 0.9 1 171509 20082 25 200150 0.9 1 186592 13558 26 167735 0.9 1 157724 10011 27 199781 0.9 1 184992 14789 28 190820 0.9 1 178673 12147 29 182632 0.9 1 167230 15402 30 215198 0.9 1 202618 12580 31 113275 0.9 1 106714 6561 32 173656 0.9 1 163614 10042 33 150753 0.9 1 139546 11207 34 170268 0.9 1 157529 12739 35 180201 0.9 1 170423 9778 36 115107 0.9 1 109303 5804 37 150149 0.9 1 138259 11890 38 150671 0.9 1 141258 9413 39 151170 0.9 1 141117 10053 40 134784 0.9 1 128831 5953 41 128562 0.9 1 119483 9079 42 150333 0.9 1 141358 8975 43 173094 0.9 1 160287 12807 44 70502 0.9 1 65481 5021 45 198421 0.9 1 190001 8420 46 37423 0.9 1 34406 3017 47 111576 0.9 1 106029 5547 48 145312 0.9 1 137831 7481 49 81528 0.9 1 77714 3814 50 107506 0.9 1 101046 6460 51 103158 0.9 1 97157 6001 52 95115 0.9 1 90369 4746 53 94271 0.9 1 89510 4761 54 100517 0.9 1 93669 6848 55 125140 0.9 1 119468 5672 56 52148 0.9 1 49411 2737 57 101537 0.9 1 96540 4997 58 115261 0.9 1 110797 4464 59 40008 0.9 1 37513 2495 60 88506 0.9 1 85648 2858 61 54004 0.9 1 51625 2379 62 74169 0.9 1 70433 3736 63 86338 0.9 1 83166 3172 64 69046 0.9 1 66762 2284 65 36461 0.9 1 34464 1997 66 64976 0.9 1 62307 2669 67 71833 0.9 1 68896 2937 68 75133 0.9 1 71995 3138 69 61629 0.9 1 58539 3090 70 106437 0.9 1 102218 4219 71 8345 0.9 1 7701 644 72 9712 0.9 1 9143 569 73 27497 0.9 1 26423 1074 74 35396 0.9 1 34141 1255 75 37367 0.9 1 35964 1403 76 36748 0.9 1 35278 1470 77 35227 0.9 1 33853 1374 78 33956 0.9 1 32589 1367 79 32308 0.9 1 31005 1303 80 30581 0.9 1 29306 1275 81 28615 0.9 1 27386 1229 82 26906 0.9 1 25903 1003 83 25237 0.9 1 24201 1036 84 23581 0.9 1 22640 941 85 21902 0.9 1 20974 928 86 20140 0.9 1 19311 829 87 19254 0.9 1 18477 777 88 18048 0.9 1 17343 705 89 16719 0.9 1 16062 657 90 15185 0.9 1 14570 615 91 14500 0.9 1 13938 562 92 13250 0.9 1 12735 515 93 12535 0.9 1 12045 490 94 11234 0.9 1 10810 424 95 10409 0.9 1 9953 456 96 9578 0.9 1 9172 406 97 8957 0.9 1 8616 341 98 8148 0.9 1 7840 308 99 7318 0.9 1 7034 284 100 6732 0.9 1 6431 301 101 6133 0.9 1 5860 273 102 5525 0.9 1 5305 220 103 4746 0.9 1 4533 213 104 4453 0.9 1 4266 187 105 3947 0.9 1 3782 165 106 3460 0.9 1 3305 155 107 3022 0.9 1 2886 136 108 2624 0.9 1 2500 124 109 2343 0.9 1 2226 117 110 2035 0.9 1 1928 107 111 1751 0.9 1 1639 112 112 1499 0.9 1 1424 75 113 1300 0.9 1 1243 57 114 1057 0.9 1 979 78 115 915 0.9 1 861 54 116 719 0.9 1 675 44 117 656 0.9 1 608 48 118 522 0.9 1 485 37 119 480 0.9 1 440 40 120 424 0.9 1 376 48 121 387 0.9 1 342 45 122 270 0.9 1 242 28 123 260 0.9 1 230 30 124 213 0.9 1 178 35 125 201 0.9 1 172 29 126 206 0.9 1 179 27 127 186 0.9 1 153 33 128 161 0.9 1 139 22 129 123 0.9 1 106 17 130 133 0.9 1 107 26 131 134 0.9 1 116 18 132 94 0.9 1 80 14 133 94 0.9 1 78 16 134 86 0.9 1 76 10 135 118 0.9 1 104 14 136 67 0.9 1 55 12 137 68 0.9 1 57 11 138 44 0.9 1 34 10 139 66 0.9 1 52 14 140 65 0.9 1 47 18 141 63 0.9 1 45 18 142 56 0.9 1 41 15 143 49 0.9 1 35 14 144 59 0.9 1 32 27 145 44 0.9 1 31 13 146 36 0.9 1 22 14 147 50 0.9 1 29 21 148 54 0.9 1 23 31 149 64 0.9 1 43 21 150 42 0.9 1 22 20 151 908 0.9 1 26 882 RUN STATISTICS FOR INPUT FILE: ERR12308505_2.fastq.gz ============================================= 61359468 sequences processed in total