Bismark report for: ERR10684020_val_1.fq.gz and ERR10684020_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 129852993 Number of paired-end alignments with a unique best hit: 72186546 Mapping efficiency: 55.6% Sequence pairs with no alignments under any condition: 43758192 Sequence pairs did not map uniquely: 13908255 Sequence pairs which were discarded because genomic sequence could not be extracted: 27 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 36101759 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 36084760 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 3514322829 Total methylated C's in CpG context: 67723467 Total methylated C's in CHG context: 6535362 Total methylated C's in CHH context: 26383047 Total methylated C's in Unknown context: 236718 Total unmethylated C's in CpG context: 355271907 Total unmethylated C's in CHG context: 586179654 Total unmethylated C's in CHH context: 2472229392 Total unmethylated C's in Unknown context: 10027076 C methylated in CpG context: 16.0% C methylated in CHG context: 1.1% C methylated in CHH context: 1.1% C methylated in Unknown context (CN or CHN): 2.3% Bismark completed in 0d 9h 26m 21s