SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684020_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 113393). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684020_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2211.460 s (17.020 µs/read; 3.53 M reads/minute). === Summary === Total reads processed: 129,934,698 Reads with adapters: 61,904,926 (47.6%) Reads written (passing filters): 129,934,698 (100.0%) Total basepairs processed: 19,620,139,398 bp Quality-trimmed: 42,817,738 bp (0.2%) Total written (filtered): 18,829,551,063 bp (96.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 61904926 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.3% C: 0.5% G: 14.0% T: 49.2% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 35843919 32483674.5 0 35843919 2 5322915 8120918.6 0 5322915 3 2318020 2030229.7 0 2318020 4 1117293 507557.4 0 1117293 5 327540 126889.4 0 327540 6 274237 31722.3 0 274237 7 258411 7930.6 0 258411 8 293331 1982.6 0 293331 9 291917 495.7 0 290530 1387 10 274662 123.9 1 251466 23196 11 308455 31.0 1 271489 36966 12 315265 7.7 1 281109 34156 13 268265 1.9 1 244608 23657 14 296824 1.9 1 263674 33150 15 290105 1.9 1 263167 26938 16 290691 1.9 1 258758 31933 17 324774 1.9 1 285305 39469 18 345394 1.9 1 317053 28341 19 187701 1.9 1 171250 16451 20 272891 1.9 1 250044 22847 21 281954 1.9 1 255437 26517 22 258464 1.9 1 238397 20067 23 285346 1.9 1 259199 26147 24 282447 1.9 1 249370 33077 25 273429 1.9 1 251011 22418 26 238975 1.9 1 221057 17918 27 277075 1.9 1 252889 24186 28 245562 1.9 1 227330 18232 29 270815 1.9 1 242903 27912 30 310649 1.9 1 288753 21896 31 174987 1.9 1 162050 12937 32 254329 1.9 1 235636 18693 33 234797 1.9 1 215625 19172 34 246331 1.9 1 225978 20353 35 239128 1.9 1 220399 18729 36 254822 1.9 1 235537 19285 37 213018 1.9 1 197204 15814 38 237255 1.9 1 219325 17930 39 269413 1.9 1 249757 19656 40 185694 1.9 1 168137 17557 41 230651 1.9 1 215154 15497 42 247208 1.9 1 228384 18824 43 332982 1.9 1 291145 41837 44 152139 1.9 1 135152 16987 45 384128 1.9 1 358441 25687 46 65469 1.9 1 57720 7749 47 206735 1.9 1 192364 14371 48 633435 1.9 1 600577 32858 49 161589 1.9 1 150406 11183 50 260794 1.9 1 243441 17353 51 338199 1.9 1 317323 20876 52 228754 1.9 1 217949 10805 53 95440 1.9 1 91120 4320 54 317263 1.9 1 305547 11716 55 384327 1.9 1 372736 11591 56 72534 1.9 1 68567 3967 57 162031 1.9 1 157330 4701 58 39581 1.9 1 37178 2403 59 83352 1.9 1 79099 4253 60 31310 1.9 1 29571 1739 61 19338 1.9 1 17631 1707 62 193899 1.9 1 186602 7297 63 82803 1.9 1 80336 2467 64 21949 1.9 1 20410 1539 65 41801 1.9 1 39229 2572 66 166527 1.9 1 160622 5905 67 63226 1.9 1 60127 3099 68 118899 1.9 1 113319 5580 69 115561 1.9 1 109983 5578 70 130567 1.9 1 123711 6856 71 15373 1.9 1 13853 1520 72 27834 1.9 1 26444 1390 73 66763 1.9 1 64163 2600 74 89668 1.9 1 86225 3443 75 96513 1.9 1 92663 3850 76 96719 1.9 1 92775 3944 77 95143 1.9 1 91058 4085 78 92732 1.9 1 88608 4124 79 89337 1.9 1 85604 3733 80 86219 1.9 1 82402 3817 81 83839 1.9 1 80194 3645 82 79940 1.9 1 76510 3430 83 77813 1.9 1 74351 3462 84 74435 1.9 1 71219 3216 85 72074 1.9 1 69082 2992 86 68965 1.9 1 66065 2900 87 66212 1.9 1 63475 2737 88 63180 1.9 1 60600 2580 89 61036 1.9 1 58601 2435 90 57977 1.9 1 55675 2302 91 55278 1.9 1 52951 2327 92 52775 1.9 1 50604 2171 93 50360 1.9 1 48411 1949 94 47877 1.9 1 45928 1949 95 45772 1.9 1 44022 1750 96 43341 1.9 1 41612 1729 97 40835 1.9 1 39186 1649 98 38826 1.9 1 37361 1465 99 36115 1.9 1 34777 1338 100 34058 1.9 1 32795 1263 101 32017 1.9 1 30862 1155 102 30155 1.9 1 29020 1135 103 27918 1.9 1 26842 1076 104 25336 1.9 1 24461 875 105 23145 1.9 1 22316 829 106 21833 1.9 1 21059 774 107 19487 1.9 1 18823 664 108 17855 1.9 1 17223 632 109 15903 1.9 1 15358 545 110 14131 1.9 1 13627 504 111 12026 1.9 1 11624 402 112 11100 1.9 1 10696 404 113 9345 1.9 1 9031 314 114 8078 1.9 1 7797 281 115 6907 1.9 1 6699 208 116 5768 1.9 1 5560 208 117 4598 1.9 1 4438 160 118 3738 1.9 1 3596 142 119 3056 1.9 1 2954 102 120 9290 1.9 1 9053 237 121 2561 1.9 1 2474 87 122 1311 1.9 1 1260 51 123 908 1.9 1 856 52 124 633 1.9 1 588 45 125 431 1.9 1 399 32 126 280 1.9 1 262 18 127 208 1.9 1 192 16 128 127 1.9 1 115 12 129 106 1.9 1 94 12 130 78 1.9 1 74 4 131 77 1.9 1 66 11 132 51 1.9 1 45 6 133 62 1.9 1 54 8 134 44 1.9 1 33 11 135 48 1.9 1 41 7 136 47 1.9 1 34 13 137 35 1.9 1 29 6 138 35 1.9 1 24 11 139 34 1.9 1 19 15 140 19 1.9 1 15 4 141 32 1.9 1 25 7 142 36 1.9 1 23 13 143 26 1.9 1 10 16 144 22 1.9 1 11 11 145 42 1.9 1 26 16 146 26 1.9 1 12 14 147 27 1.9 1 14 13 148 32 1.9 1 10 22 149 29 1.9 1 12 17 150 165 1.9 1 3 162 151 49108 1.9 1 117 48991 RUN STATISTICS FOR INPUT FILE: ERR10684020_1.fastq.gz ============================================= 129934698 sequences processed in total