SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684019_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 83127). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684019_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1844.641 s (17.677 µs/read; 3.39 M reads/minute). === Summary === Total reads processed: 104,352,237 Reads with adapters: 47,409,317 (45.4%) Reads written (passing filters): 104,352,237 (100.0%) Total basepairs processed: 15,757,187,787 bp Quality-trimmed: 42,137,185 bp (0.3%) Total written (filtered): 15,281,268,873 bp (97.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 47409317 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.0% C: 0.7% G: 14.4% T: 48.9% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 29361110 26088059.2 0 29361110 2 4540862 6522014.8 0 4540862 3 1924677 1630503.7 0 1924677 4 893013 407625.9 0 893013 5 243689 101906.5 0 243689 6 207035 25476.6 0 207035 7 195489 6369.2 0 195489 8 218690 1592.3 0 218690 9 213746 398.1 0 212666 1080 10 204791 99.5 1 187033 17758 11 222829 24.9 1 196517 26312 12 230156 6.2 1 205040 25116 13 195990 1.6 1 178556 17434 14 213803 1.6 1 190093 23710 15 206327 1.6 1 187527 18800 16 205468 1.6 1 182724 22744 17 230578 1.6 1 203672 26906 18 242867 1.6 1 223140 19727 19 131420 1.6 1 120072 11348 20 189021 1.6 1 173467 15554 21 192625 1.6 1 174964 17661 22 178785 1.6 1 164560 14225 23 196678 1.6 1 179179 17499 24 188227 1.6 1 166023 22204 25 183725 1.6 1 169015 14710 26 154856 1.6 1 143757 11099 27 183020 1.6 1 166849 16171 28 167854 1.6 1 154981 12873 29 172401 1.6 1 155265 17136 30 201438 1.6 1 187079 14359 31 112773 1.6 1 104421 8352 32 160810 1.6 1 149068 11742 33 151136 1.6 1 138433 12703 34 166337 1.6 1 151635 14702 35 135454 1.6 1 127426 8028 36 159663 1.6 1 145638 14025 37 137358 1.6 1 129069 8289 38 144492 1.6 1 130052 14440 39 135611 1.6 1 126063 9548 40 138557 1.6 1 128288 10269 41 152387 1.6 1 140974 11413 42 139863 1.6 1 129057 10806 43 181776 1.6 1 158238 23538 44 107387 1.6 1 95912 11475 45 245186 1.6 1 229300 15886 46 48467 1.6 1 42292 6175 47 125799 1.6 1 116270 9529 48 950328 1.6 1 916331 33997 49 215429 1.6 1 204962 10467 50 54545 1.6 1 50627 3918 51 140638 1.6 1 133582 7056 52 110678 1.6 1 105462 5216 53 10546 1.6 1 9225 1321 54 73410 1.6 1 71040 2370 55 49976 1.6 1 47919 2057 56 18859 1.6 1 17566 1293 57 10922 1.6 1 10216 706 58 7869 1.6 1 6964 905 59 14520 1.6 1 13352 1168 60 5357 1.6 1 4581 776 61 8171 1.6 1 7296 875 62 64065 1.6 1 61757 2308 63 7097 1.6 1 6502 595 64 11038 1.6 1 10296 742 65 12790 1.6 1 11861 929 66 59957 1.6 1 57737 2220 67 10662 1.6 1 9828 834 68 35209 1.6 1 33505 1704 69 40999 1.6 1 39058 1941 70 54246 1.6 1 51727 2519 71 11368 1.6 1 10436 932 72 8076 1.6 1 7516 560 73 22051 1.6 1 21153 898 74 36781 1.6 1 35409 1372 75 44108 1.6 1 42463 1645 76 45808 1.6 1 43995 1813 77 45583 1.6 1 43688 1895 78 44400 1.6 1 42579 1821 79 43323 1.6 1 41579 1744 80 41929 1.6 1 40182 1747 81 40170 1.6 1 38527 1643 82 38877 1.6 1 37271 1606 83 37483 1.6 1 35932 1551 84 35514 1.6 1 34032 1482 85 34076 1.6 1 32712 1364 86 33104 1.6 1 31771 1333 87 31902 1.6 1 30607 1295 88 30405 1.6 1 29203 1202 89 28794 1.6 1 27608 1186 90 27621 1.6 1 26582 1039 91 26512 1.6 1 25481 1031 92 25046 1.6 1 24100 946 93 23914 1.6 1 23003 911 94 22779 1.6 1 21961 818 95 21682 1.6 1 20900 782 96 20566 1.6 1 19793 773 97 19172 1.6 1 18478 694 98 18769 1.6 1 18057 712 99 17431 1.6 1 16825 606 100 16098 1.6 1 15535 563 101 15182 1.6 1 14665 517 102 14370 1.6 1 13870 500 103 13429 1.6 1 12982 447 104 12170 1.6 1 11757 413 105 11444 1.6 1 11053 391 106 10484 1.6 1 10112 372 107 9653 1.6 1 9329 324 108 8708 1.6 1 8427 281 109 7884 1.6 1 7606 278 110 6909 1.6 1 6667 242 111 6205 1.6 1 5974 231 112 5640 1.6 1 5466 174 113 4986 1.6 1 4816 170 114 4392 1.6 1 4201 191 115 3614 1.6 1 3493 121 116 3112 1.6 1 3006 106 117 2679 1.6 1 2584 95 118 2175 1.6 1 2102 73 119 1894 1.6 1 1817 77 120 6031 1.6 1 5902 129 121 1675 1.6 1 1606 69 122 939 1.6 1 893 46 123 733 1.6 1 692 41 124 498 1.6 1 468 30 125 404 1.6 1 373 31 126 326 1.6 1 312 14 127 278 1.6 1 253 25 128 208 1.6 1 187 21 129 177 1.6 1 164 13 130 162 1.6 1 145 17 131 162 1.6 1 140 22 132 105 1.6 1 93 12 133 126 1.6 1 104 22 134 94 1.6 1 86 8 135 122 1.6 1 105 17 136 92 1.6 1 83 9 137 122 1.6 1 100 22 138 78 1.6 1 62 16 139 84 1.6 1 73 11 140 79 1.6 1 57 22 141 80 1.6 1 70 10 142 60 1.6 1 42 18 143 49 1.6 1 36 13 144 51 1.6 1 33 18 145 80 1.6 1 48 32 146 59 1.6 1 37 22 147 70 1.6 1 39 31 148 57 1.6 1 27 30 149 75 1.6 1 43 32 150 167 1.6 1 29 138 151 56265 1.6 1 135 56130 RUN STATISTICS FOR INPUT FILE: ERR10684019_1.fastq.gz ============================================= 104352237 sequences processed in total