SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684018_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 85236). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684018_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 2510.107 s (20.625 µs/read; 2.91 M reads/minute). === Summary === Total reads processed: 121,703,060 Reads with adapters: 55,795,423 (45.8%) Reads written (passing filters): 121,703,060 (100.0%) Total basepairs processed: 18,377,162,060 bp Quality-trimmed: 46,997,677 bp (0.3%) Total written (filtered): 17,808,462,055 bp (96.9%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 55795423 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.1% C: 0.8% G: 14.2% T: 48.8% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 34323588 30425765.0 0 34323588 2 5259250 7606441.2 0 5259250 3 2244621 1901610.3 0 2244621 4 1035448 475402.6 0 1035448 5 296394 118850.6 0 296394 6 249176 29712.7 0 249176 7 233730 7428.2 0 233730 8 263416 1857.0 0 263416 9 259387 464.3 0 257238 2149 10 244968 116.1 1 223654 21314 11 271287 29.0 1 238341 32946 12 275670 7.3 1 245203 30467 13 231152 1.8 1 209627 21525 14 260582 1.8 1 230077 30505 15 250256 1.8 1 225985 24271 16 250344 1.8 1 221024 29320 17 277631 1.8 1 243140 34491 18 293628 1.8 1 269433 24195 19 159250 1.8 1 143752 15498 20 230845 1.8 1 210364 20481 21 232380 1.8 1 210213 22167 22 213511 1.8 1 195426 18085 23 233565 1.8 1 211095 22470 24 230883 1.8 1 202873 28010 25 223525 1.8 1 204532 18993 26 193441 1.8 1 177653 15788 27 222215 1.8 1 201917 20298 28 200257 1.8 1 183971 16286 29 214498 1.8 1 191382 23116 30 244638 1.8 1 226411 18227 31 136587 1.8 1 125284 11303 32 193901 1.8 1 178657 15244 33 184266 1.8 1 167558 16708 34 192729 1.8 1 174407 18322 35 180331 1.8 1 165748 14583 36 185390 1.8 1 170906 14484 37 160803 1.8 1 148361 12442 38 177902 1.8 1 163127 14775 39 182406 1.8 1 169124 13282 40 152115 1.8 1 136642 15473 41 159913 1.8 1 148946 10967 42 173693 1.8 1 159139 14554 43 218203 1.8 1 189210 28993 44 145916 1.8 1 128638 17278 45 265220 1.8 1 247665 17555 46 77258 1.8 1 67045 10213 47 174483 1.8 1 152108 22375 48 1181986 1.8 1 1136814 45172 49 298368 1.8 1 284640 13728 50 48793 1.8 1 43407 5386 51 172670 1.8 1 162754 9916 52 143196 1.8 1 136795 6401 53 11595 1.8 1 9681 1914 54 68456 1.8 1 65677 2779 55 54885 1.8 1 52561 2324 56 21728 1.8 1 20281 1447 57 10516 1.8 1 9726 790 58 9265 1.8 1 8196 1069 59 15416 1.8 1 14133 1283 60 6681 1.8 1 5771 910 61 10306 1.8 1 8690 1616 62 68900 1.8 1 66467 2433 63 7590 1.8 1 6870 720 64 13110 1.8 1 12166 944 65 15445 1.8 1 13801 1644 66 67120 1.8 1 64662 2458 67 11998 1.8 1 10672 1326 68 38858 1.8 1 36507 2351 69 47629 1.8 1 44764 2865 70 64353 1.8 1 61232 3121 71 16209 1.8 1 14821 1388 72 9272 1.8 1 8377 895 73 23515 1.8 1 22108 1407 74 41098 1.8 1 39135 1963 75 51766 1.8 1 49367 2399 76 55018 1.8 1 52414 2604 77 54933 1.8 1 52193 2740 78 53414 1.8 1 50777 2637 79 52000 1.8 1 49430 2570 80 49902 1.8 1 47446 2456 81 48692 1.8 1 46400 2292 82 46844 1.8 1 44540 2304 83 44467 1.8 1 42300 2167 84 43351 1.8 1 41171 2180 85 41367 1.8 1 39293 2074 86 39777 1.8 1 37930 1847 87 38260 1.8 1 36402 1858 88 36572 1.8 1 34849 1723 89 34843 1.8 1 33180 1663 90 33223 1.8 1 31648 1575 91 31969 1.8 1 30431 1538 92 30365 1.8 1 28923 1442 93 29222 1.8 1 27841 1381 94 27380 1.8 1 26094 1286 95 26008 1.8 1 24806 1202 96 24667 1.8 1 23526 1141 97 23502 1.8 1 22449 1053 98 22591 1.8 1 21626 965 99 21214 1.8 1 20231 983 100 19836 1.8 1 18975 861 101 18261 1.8 1 17510 751 102 17556 1.8 1 16832 724 103 15918 1.8 1 15233 685 104 14832 1.8 1 14135 697 105 13746 1.8 1 13097 649 106 12688 1.8 1 12176 512 107 11647 1.8 1 11164 483 108 10506 1.8 1 10042 464 109 9438 1.8 1 9071 367 110 8657 1.8 1 8317 340 111 7707 1.8 1 7408 299 112 6711 1.8 1 6410 301 113 5815 1.8 1 5579 236 114 5171 1.8 1 4945 226 115 4430 1.8 1 4238 192 116 3670 1.8 1 3509 161 117 3146 1.8 1 2995 151 118 2448 1.8 1 2341 107 119 2096 1.8 1 2008 88 120 7410 1.8 1 7187 223 121 2047 1.8 1 1974 73 122 1040 1.8 1 991 49 123 732 1.8 1 677 55 124 490 1.8 1 461 29 125 371 1.8 1 348 23 126 280 1.8 1 256 24 127 181 1.8 1 157 24 128 160 1.8 1 135 25 129 130 1.8 1 105 25 130 100 1.8 1 83 17 131 94 1.8 1 75 19 132 58 1.8 1 43 15 133 73 1.8 1 67 6 134 58 1.8 1 46 12 135 73 1.8 1 52 21 136 61 1.8 1 41 20 137 66 1.8 1 48 18 138 48 1.8 1 35 13 139 56 1.8 1 43 13 140 50 1.8 1 28 22 141 78 1.8 1 57 21 142 41 1.8 1 19 22 143 54 1.8 1 34 20 144 36 1.8 1 14 22 145 47 1.8 1 28 19 146 44 1.8 1 16 28 147 45 1.8 1 20 25 148 38 1.8 1 15 23 149 59 1.8 1 29 30 150 183 1.8 1 10 173 151 65984 1.8 1 129 65855 RUN STATISTICS FOR INPUT FILE: ERR10684018_2.fastq.gz ============================================= 121703060 sequences processed in total