SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684017_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 84293). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684017_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 1813.733 s (20.908 µs/read; 2.87 M reads/minute). === Summary === Total reads processed: 86,749,190 Reads with adapters: 39,369,096 (45.4%) Reads written (passing filters): 86,749,190 (100.0%) Total basepairs processed: 13,099,127,690 bp Quality-trimmed: 35,095,484 bp (0.3%) Total written (filtered): 12,688,632,374 bp (96.9%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 39369096 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 34.9% C: 1.2% G: 15.6% T: 48.2% none/other: 0.2% Overview of removed sequences length count expect max.err error counts 1 23651231 21687297.5 0 23651231 2 3949812 5421824.4 0 3949812 3 1659303 1355456.1 0 1659303 4 744109 338864.0 0 744109 5 225939 84716.0 0 225939 6 194506 21179.0 0 194506 7 182541 5294.8 0 182541 8 203168 1323.7 0 203168 9 195712 330.9 0 194159 1553 10 186647 82.7 1 168514 18133 11 202294 20.7 1 176098 26196 12 207023 5.2 1 182095 24928 13 182051 1.3 1 163144 18907 14 192341 1.3 1 168825 23516 15 193344 1.3 1 172517 20827 16 185256 1.3 1 162204 23052 17 210401 1.3 1 182237 28164 18 228426 1.3 1 207058 21368 19 114090 1.3 1 102161 11929 20 173233 1.3 1 156202 17031 21 164790 1.3 1 148070 16720 22 157716 1.3 1 142351 15365 23 171320 1.3 1 153115 18205 24 166847 1.3 1 145249 21598 25 165016 1.3 1 149109 15907 26 138657 1.3 1 126120 12537 27 163907 1.3 1 147339 16568 28 153871 1.3 1 139506 14365 29 154097 1.3 1 135809 18288 30 177549 1.3 1 163017 14532 31 95197 1.3 1 86357 8840 32 138208 1.3 1 125935 12273 33 129963 1.3 1 116736 13227 34 139055 1.3 1 124118 14937 35 132558 1.3 1 120746 11812 36 122923 1.3 1 112502 10421 37 121884 1.3 1 109605 12279 38 131632 1.3 1 118827 12805 39 147443 1.3 1 131750 15693 40 91915 1.3 1 84102 7813 41 117951 1.3 1 108108 9843 42 120819 1.3 1 108867 11952 43 168515 1.3 1 145512 23003 44 94452 1.3 1 82228 12224 45 182102 1.3 1 169475 12627 46 41008 1.3 1 35204 5804 47 102719 1.3 1 92054 10665 48 337566 1.3 1 318076 19490 49 95805 1.3 1 87603 8202 50 111673 1.3 1 102878 8795 51 170342 1.3 1 159249 11093 52 109121 1.3 1 102557 6564 53 36748 1.3 1 33767 2981 54 135594 1.3 1 129061 6533 55 147869 1.3 1 142422 5447 56 33184 1.3 1 30567 2617 57 53292 1.3 1 51431 1861 58 15476 1.3 1 13884 1592 59 35353 1.3 1 33219 2134 60 11400 1.3 1 10415 985 61 11182 1.3 1 9252 1930 62 79728 1.3 1 75923 3805 63 27172 1.3 1 26106 1066 64 10547 1.3 1 9400 1147 65 19519 1.3 1 17603 1916 66 67398 1.3 1 64179 3219 67 23658 1.3 1 21948 1710 68 45913 1.3 1 43148 2765 69 46478 1.3 1 43627 2851 70 57698 1.3 1 54556 3142 71 8443 1.3 1 7445 998 72 11401 1.3 1 10434 967 73 25778 1.3 1 24241 1537 74 35183 1.3 1 33279 1904 75 38273 1.3 1 36221 2052 76 38397 1.3 1 36294 2103 77 37662 1.3 1 35398 2264 78 36614 1.3 1 34455 2159 79 34900 1.3 1 32782 2118 80 33353 1.3 1 31483 1870 81 32555 1.3 1 30683 1872 82 30821 1.3 1 29064 1757 83 29598 1.3 1 27950 1648 84 28172 1.3 1 26566 1606 85 26869 1.3 1 25396 1473 86 25891 1.3 1 24476 1415 87 24572 1.3 1 23323 1249 88 23565 1.3 1 22309 1256 89 22225 1.3 1 20992 1233 90 21618 1.3 1 20484 1134 91 20135 1.3 1 18994 1141 92 19048 1.3 1 18041 1007 93 18400 1.3 1 17379 1021 94 16907 1.3 1 16014 893 95 16077 1.3 1 15277 800 96 15242 1.3 1 14451 791 97 14515 1.3 1 13752 763 98 13571 1.3 1 12892 679 99 12769 1.3 1 12118 651 100 11985 1.3 1 11349 636 101 11159 1.3 1 10565 594 102 10262 1.3 1 9721 541 103 9796 1.3 1 9324 472 104 9024 1.3 1 8571 453 105 8129 1.3 1 7701 428 106 7391 1.3 1 7005 386 107 6832 1.3 1 6466 366 108 6197 1.3 1 5931 266 109 5819 1.3 1 5573 246 110 5060 1.3 1 4793 267 111 4772 1.3 1 4544 228 112 4189 1.3 1 3992 197 113 3659 1.3 1 3461 198 114 3179 1.3 1 3017 162 115 2698 1.3 1 2562 136 116 2322 1.3 1 2199 123 117 2233 1.3 1 2127 106 118 1824 1.3 1 1723 101 119 1653 1.3 1 1542 111 120 9490 1.3 1 9206 284 121 2020 1.3 1 1935 85 122 949 1.3 1 890 59 123 814 1.3 1 766 48 124 578 1.3 1 523 55 125 443 1.3 1 397 46 126 348 1.3 1 319 29 127 251 1.3 1 228 23 128 211 1.3 1 185 26 129 258 1.3 1 223 35 130 184 1.3 1 162 22 131 155 1.3 1 131 24 132 160 1.3 1 138 22 133 145 1.3 1 120 25 134 116 1.3 1 95 21 135 142 1.3 1 122 20 136 105 1.3 1 85 20 137 146 1.3 1 122 24 138 90 1.3 1 66 24 139 127 1.3 1 103 24 140 94 1.3 1 74 20 141 130 1.3 1 105 25 142 85 1.3 1 59 26 143 74 1.3 1 47 27 144 82 1.3 1 49 33 145 96 1.3 1 68 28 146 93 1.3 1 51 42 147 88 1.3 1 57 31 148 72 1.3 1 44 28 149 78 1.3 1 54 24 150 222 1.3 1 38 184 151 64256 1.3 1 169 64087 RUN STATISTICS FOR INPUT FILE: ERR10684017_2.fastq.gz ============================================= 86749190 sequences processed in total