SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684016_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 86967). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684016_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 1814.073 s (18.423 µs/read; 3.26 M reads/minute). === Summary === Total reads processed: 98,465,942 Reads with adapters: 45,067,222 (45.8%) Reads written (passing filters): 98,465,942 (100.0%) Total basepairs processed: 14,868,357,242 bp Quality-trimmed: 28,981,940 bp (0.2%) Total written (filtered): 14,395,083,454 bp (96.8%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 45067222 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 35.8% C: 0.8% G: 14.6% T: 48.6% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 27449659 24616485.5 0 27449659 2 4316218 6154121.4 0 4316218 3 1830851 1538530.3 0 1830851 4 844141 384632.6 0 844141 5 240002 96158.1 0 240002 6 202921 24039.5 0 202921 7 193129 6009.9 0 193129 8 215396 1502.5 0 215396 9 207423 375.6 0 205186 2237 10 201097 93.9 1 182090 19007 11 217134 23.5 1 190230 26904 12 223935 5.9 1 197679 26256 13 190668 1.5 1 171547 19121 14 209050 1.5 1 183646 25404 15 203384 1.5 1 182162 21222 16 200756 1.5 1 176254 24502 17 223377 1.5 1 194239 29138 18 238561 1.5 1 217162 21399 19 128539 1.5 1 115435 13104 20 187846 1.5 1 170211 17635 21 185256 1.5 1 166758 18498 22 177359 1.5 1 160810 16549 23 192130 1.5 1 172762 19368 24 182338 1.5 1 159660 22678 25 184835 1.5 1 167351 17484 26 155378 1.5 1 141948 13430 27 182479 1.5 1 164129 18350 28 164104 1.5 1 149567 14537 29 171010 1.5 1 151773 19237 30 200366 1.5 1 184159 16207 31 113947 1.5 1 103460 10487 32 163752 1.5 1 149997 13755 33 148438 1.5 1 133572 14866 34 161477 1.5 1 146551 14926 35 136934 1.5 1 125842 11092 36 152223 1.5 1 139179 13044 37 137698 1.5 1 126555 11143 38 141571 1.5 1 128449 13122 39 145070 1.5 1 132199 12871 40 157678 1.5 1 145068 12610 41 116854 1.5 1 104398 12456 42 126598 1.5 1 116835 9763 43 159072 1.5 1 143678 15394 44 123874 1.5 1 109655 14219 45 191015 1.5 1 180275 10740 46 56612 1.5 1 50388 6224 47 87691 1.5 1 82155 5536 48 134855 1.5 1 125107 9748 49 97389 1.5 1 90461 6928 50 105325 1.5 1 96953 8372 51 115438 1.5 1 104563 10875 52 113912 1.5 1 105145 8767 53 93130 1.5 1 87654 5476 54 96418 1.5 1 89135 7283 55 150134 1.5 1 141073 9061 56 67423 1.5 1 61533 5890 57 113832 1.5 1 107687 6145 58 116833 1.5 1 110781 6052 59 65908 1.5 1 60986 4922 60 66308 1.5 1 63624 2684 61 44738 1.5 1 40945 3793 62 112903 1.5 1 106175 6728 63 85826 1.5 1 82530 3296 64 59395 1.5 1 56433 2962 65 38275 1.5 1 34884 3391 66 91839 1.5 1 86947 4892 67 79637 1.5 1 75413 4224 68 95073 1.5 1 90328 4745 69 66630 1.5 1 62697 3933 70 63655 1.5 1 60021 3634 71 14544 1.5 1 13412 1132 72 23089 1.5 1 21659 1430 73 41995 1.5 1 39767 2228 74 48486 1.5 1 46052 2434 75 49349 1.5 1 46802 2547 76 48255 1.5 1 45739 2516 77 47625 1.5 1 44979 2646 78 45346 1.5 1 42801 2545 79 43653 1.5 1 41338 2315 80 42051 1.5 1 39899 2152 81 40141 1.5 1 38033 2108 82 38656 1.5 1 36694 1962 83 37207 1.5 1 35248 1959 84 36300 1.5 1 34401 1899 85 34328 1.5 1 32548 1780 86 32861 1.5 1 31138 1723 87 31424 1.5 1 29844 1580 88 29810 1.5 1 28351 1459 89 28450 1.5 1 27045 1405 90 27173 1.5 1 25800 1373 91 25627 1.5 1 24334 1293 92 24664 1.5 1 23493 1171 93 23353 1.5 1 22245 1108 94 22370 1.5 1 21301 1069 95 20878 1.5 1 19883 995 96 19984 1.5 1 19002 982 97 18982 1.5 1 18066 916 98 17718 1.5 1 16836 882 99 16824 1.5 1 16027 797 100 15799 1.5 1 15035 764 101 14563 1.5 1 13874 689 102 13759 1.5 1 13134 625 103 12816 1.5 1 12208 608 104 11660 1.5 1 11130 530 105 10681 1.5 1 10188 493 106 9768 1.5 1 9355 413 107 9157 1.5 1 8759 398 108 8169 1.5 1 7774 395 109 7277 1.5 1 6980 297 110 6518 1.5 1 6241 277 111 5807 1.5 1 5523 284 112 5211 1.5 1 4976 235 113 4571 1.5 1 4349 222 114 4016 1.5 1 3814 202 115 3368 1.5 1 3230 138 116 2987 1.5 1 2847 140 117 2573 1.5 1 2434 139 118 2159 1.5 1 2042 117 119 1794 1.5 1 1676 118 120 5285 1.5 1 5076 209 121 1587 1.5 1 1524 63 122 992 1.5 1 938 54 123 801 1.5 1 747 54 124 594 1.5 1 561 33 125 480 1.5 1 443 37 126 450 1.5 1 408 42 127 337 1.5 1 301 36 128 226 1.5 1 203 23 129 261 1.5 1 232 29 130 192 1.5 1 162 30 131 228 1.5 1 202 26 132 157 1.5 1 137 20 133 170 1.5 1 150 20 134 150 1.5 1 133 17 135 166 1.5 1 146 20 136 134 1.5 1 112 22 137 187 1.5 1 169 18 138 113 1.5 1 97 16 139 154 1.5 1 133 21 140 91 1.5 1 66 25 141 128 1.5 1 110 18 142 94 1.5 1 68 26 143 97 1.5 1 74 23 144 84 1.5 1 54 30 145 102 1.5 1 68 34 146 90 1.5 1 52 38 147 78 1.5 1 48 30 148 76 1.5 1 51 25 149 112 1.5 1 64 48 150 195 1.5 1 27 168 151 54893 1.5 1 143 54750 RUN STATISTICS FOR INPUT FILE: ERR10684016_2.fastq.gz ============================================= 98465942 sequences processed in total