SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684015_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 113330). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684015_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2395.325 s (17.526 µs/read; 3.42 M reads/minute). === Summary === Total reads processed: 136,675,754 Reads with adapters: 65,482,478 (47.9%) Reads written (passing filters): 136,675,754 (100.0%) Total basepairs processed: 20,638,038,854 bp Quality-trimmed: 30,608,864 bp (0.1%) Total written (filtered): 19,842,745,147 bp (96.1%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 65482478 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.0% C: 0.3% G: 14.2% T: 49.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 37727720 34168938.5 0 37727720 2 5560201 8542234.6 0 5560201 3 2466997 2135558.7 0 2466997 4 1225623 533889.7 0 1225623 5 400028 133472.4 0 400028 6 332221 33368.1 0 332221 7 310880 8342.0 0 310880 8 352018 2085.5 0 352018 9 351615 521.4 0 350296 1319 10 325667 130.3 1 300204 25463 11 363724 32.6 1 321152 42572 12 373635 8.1 1 334319 39316 13 312585 2.0 1 286836 25749 14 354302 2.0 1 315499 38803 15 335410 2.0 1 306309 29101 16 346131 2.0 1 308382 37749 17 385029 2.0 1 340646 44383 18 398481 2.0 1 367618 30863 19 220091 2.0 1 201612 18479 20 313361 2.0 1 288154 25207 21 321291 2.0 1 292009 29282 22 291177 2.0 1 270088 21089 23 323240 2.0 1 294698 28542 24 318637 2.0 1 282362 36275 25 308162 2.0 1 284649 23513 26 267543 2.0 1 248552 18991 27 313757 2.0 1 287295 26462 28 280275 2.0 1 260971 19304 29 301343 2.0 1 271604 29739 30 346025 2.0 1 322598 23427 31 190232 2.0 1 177016 13216 32 269033 2.0 1 250627 18406 33 268815 2.0 1 245165 23650 34 294445 2.0 1 271113 23332 35 263080 2.0 1 248683 14397 36 228048 2.0 1 204360 23688 37 261163 2.0 1 240846 20317 38 249708 2.0 1 232763 16945 39 276969 2.0 1 257085 19884 40 207402 2.0 1 192962 14440 41 236043 2.0 1 222015 14028 42 245421 2.0 1 232086 13335 43 243426 2.0 1 222714 20712 44 216463 2.0 1 198321 18142 45 347720 2.0 1 330665 17055 46 76833 2.0 1 70068 6765 47 186537 2.0 1 177836 8701 48 226785 2.0 1 213625 13160 49 183085 2.0 1 173094 9991 50 199293 2.0 1 186278 13015 51 205477 2.0 1 189877 15600 52 197446 2.0 1 185273 12173 53 178551 2.0 1 169967 8584 54 175125 2.0 1 165218 9907 55 250962 2.0 1 237620 13342 56 123238 2.0 1 115778 7460 57 201936 2.0 1 193207 8729 58 241912 2.0 1 231175 10737 59 111120 2.0 1 103565 7555 60 142708 2.0 1 138092 4616 61 90125 2.0 1 85328 4797 62 196242 2.0 1 186606 9636 63 164841 2.0 1 159836 5005 64 130324 2.0 1 125046 5278 65 70483 2.0 1 66484 3999 66 161315 2.0 1 154751 6564 67 166959 2.0 1 160163 6796 68 198531 2.0 1 190071 8460 69 124491 2.0 1 118149 6342 70 116714 2.0 1 110029 6685 71 28194 2.0 1 26782 1412 72 44116 2.0 1 42523 1593 73 80134 2.0 1 77318 2816 74 91638 2.0 1 88319 3319 75 93082 2.0 1 89654 3428 76 91372 2.0 1 87662 3710 77 89307 2.0 1 85622 3685 78 86035 2.0 1 82628 3407 79 83555 2.0 1 80228 3327 80 80347 2.0 1 77098 3249 81 76964 2.0 1 73827 3137 82 73704 2.0 1 70776 2928 83 70762 2.0 1 67867 2895 84 68247 2.0 1 65529 2718 85 65237 2.0 1 62780 2457 86 61906 2.0 1 59526 2380 87 59683 2.0 1 57417 2266 88 56741 2.0 1 54656 2085 89 53866 2.0 1 51920 1946 90 51349 2.0 1 49416 1933 91 48552 2.0 1 46788 1764 92 45781 2.0 1 44059 1722 93 43832 2.0 1 42219 1613 94 41097 2.0 1 39542 1555 95 38578 2.0 1 37167 1411 96 36092 2.0 1 34915 1177 97 33984 2.0 1 32825 1159 98 32199 2.0 1 31053 1146 99 30066 2.0 1 29038 1028 100 27957 2.0 1 27011 946 101 25907 2.0 1 24990 917 102 24184 2.0 1 23385 799 103 21850 2.0 1 21122 728 104 20424 2.0 1 19711 713 105 18336 2.0 1 17714 622 106 16409 2.0 1 15885 524 107 14718 2.0 1 14282 436 108 13120 2.0 1 12676 444 109 11860 2.0 1 11505 355 110 10486 2.0 1 10164 322 111 8839 2.0 1 8535 304 112 7571 2.0 1 7322 249 113 6472 2.0 1 6271 201 114 5494 2.0 1 5309 185 115 4386 2.0 1 4249 137 116 3700 2.0 1 3583 117 117 2957 2.0 1 2845 112 118 2326 2.0 1 2241 85 119 1876 2.0 1 1801 75 120 2780 2.0 1 2699 81 121 1184 2.0 1 1134 50 122 737 2.0 1 717 20 123 487 2.0 1 456 31 124 314 2.0 1 298 16 125 230 2.0 1 215 15 126 139 2.0 1 125 14 127 98 2.0 1 79 19 128 85 2.0 1 68 17 129 71 2.0 1 60 11 130 42 2.0 1 37 5 131 38 2.0 1 32 6 132 28 2.0 1 21 7 133 34 2.0 1 26 8 134 19 2.0 1 12 7 135 43 2.0 1 29 14 136 26 2.0 1 20 6 137 19 2.0 1 17 2 138 21 2.0 1 12 9 139 28 2.0 1 19 9 140 21 2.0 1 15 6 141 25 2.0 1 17 8 142 12 2.0 1 5 7 143 17 2.0 1 9 8 144 21 2.0 1 11 10 145 18 2.0 1 13 5 146 13 2.0 1 7 6 147 16 2.0 1 7 9 148 12 2.0 1 2 10 149 32 2.0 1 13 19 150 61 2.0 1 2 59 151 24345 2.0 1 48 24297 RUN STATISTICS FOR INPUT FILE: ERR10684015_1.fastq.gz ============================================= 136675754 sequences processed in total