SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684014_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 72677). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684014_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2377.487 s (19.167 µs/read; 3.13 M reads/minute). === Summary === Total reads processed: 124,042,011 Reads with adapters: 55,536,624 (44.8%) Reads written (passing filters): 124,042,011 (100.0%) Total basepairs processed: 18,730,343,661 bp Quality-trimmed: 32,387,127 bp (0.2%) Total written (filtered): 18,224,173,413 bp (97.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 55536624 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.2% C: 0.4% G: 14.2% T: 49.0% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 35394447 31010502.8 0 35394447 2 5410218 7752625.7 0 5410218 3 2284234 1938156.4 0 2284234 4 1060325 484539.1 0 1060325 5 274648 121134.8 0 274648 6 228821 30283.7 0 228821 7 212506 7570.9 0 212506 8 241186 1892.7 0 241186 9 236963 473.2 0 234888 2075 10 222444 118.3 1 202200 20244 11 247598 29.6 1 216289 31309 12 251565 7.4 1 223137 28428 13 211240 1.8 1 190992 20248 14 236479 1.8 1 208176 28303 15 225296 1.8 1 203064 22232 16 225155 1.8 1 198362 26793 17 250682 1.8 1 219314 31368 18 258273 1.8 1 236419 21854 19 146607 1.8 1 132118 14489 20 205752 1.8 1 187065 18687 21 210325 1.8 1 189297 21028 22 190981 1.8 1 174802 16179 23 210711 1.8 1 190208 20503 24 206741 1.8 1 180804 25937 25 196895 1.8 1 179792 17103 26 169608 1.8 1 155467 14141 27 199027 1.8 1 180372 18655 28 175842 1.8 1 161347 14495 29 190615 1.8 1 169775 20840 30 214560 1.8 1 198530 16030 31 120941 1.8 1 110658 10283 32 170308 1.8 1 156956 13352 33 162986 1.8 1 147845 15141 34 169553 1.8 1 153472 16081 35 162551 1.8 1 149771 12780 36 145462 1.8 1 135199 10263 37 172866 1.8 1 158969 13897 38 143074 1.8 1 126693 16381 39 148976 1.8 1 135722 13254 40 149751 1.8 1 139077 10674 41 132333 1.8 1 121071 11262 42 138854 1.8 1 129485 9369 43 180379 1.8 1 161901 18478 44 117904 1.8 1 105615 12289 45 208301 1.8 1 196390 11911 46 50614 1.8 1 44706 5908 47 107970 1.8 1 100940 7030 48 183891 1.8 1 172104 11787 49 95271 1.8 1 88115 7156 50 113275 1.8 1 104009 9266 51 151846 1.8 1 138955 12891 52 116406 1.8 1 109279 7127 53 80884 1.8 1 76415 4469 54 130030 1.8 1 121897 8133 55 210262 1.8 1 202428 7834 56 67474 1.8 1 62887 4587 57 128269 1.8 1 124157 4112 58 64449 1.8 1 61130 3319 59 84664 1.8 1 80525 4139 60 37240 1.8 1 35755 1485 61 23500 1.8 1 20942 2558 62 118788 1.8 1 113922 4866 63 72113 1.8 1 70150 1963 64 26691 1.8 1 25070 1621 65 32881 1.8 1 30405 2476 66 90957 1.8 1 87469 3488 67 53294 1.8 1 50442 2852 68 76646 1.8 1 73012 3634 69 67658 1.8 1 63964 3694 70 72951 1.8 1 69285 3666 71 9416 1.8 1 8502 914 72 17075 1.8 1 16046 1029 73 36396 1.8 1 34713 1683 74 45869 1.8 1 43784 2085 75 48094 1.8 1 45959 2135 76 48217 1.8 1 45901 2316 77 46874 1.8 1 44622 2252 78 45575 1.8 1 43440 2135 79 44376 1.8 1 42195 2181 80 42126 1.8 1 40136 1990 81 40643 1.8 1 38724 1919 82 39279 1.8 1 37462 1817 83 37717 1.8 1 35937 1780 84 36376 1.8 1 34619 1757 85 34559 1.8 1 32845 1714 86 33226 1.8 1 31720 1506 87 32308 1.8 1 30810 1498 88 30597 1.8 1 29199 1398 89 29179 1.8 1 27865 1314 90 27704 1.8 1 26413 1291 91 26689 1.8 1 25429 1260 92 25440 1.8 1 24348 1092 93 23916 1.8 1 22874 1042 94 23149 1.8 1 22121 1028 95 21766 1.8 1 20834 932 96 21042 1.8 1 20151 891 97 19931 1.8 1 19054 877 98 18881 1.8 1 18065 816 99 17534 1.8 1 16802 732 100 16563 1.8 1 15867 696 101 15853 1.8 1 15226 627 102 14556 1.8 1 13958 598 103 13889 1.8 1 13298 591 104 12766 1.8 1 12229 537 105 11696 1.8 1 11215 481 106 10754 1.8 1 10317 437 107 9944 1.8 1 9522 422 108 8877 1.8 1 8540 337 109 8026 1.8 1 7679 347 110 6981 1.8 1 6696 285 111 6416 1.8 1 6172 244 112 5723 1.8 1 5483 240 113 5098 1.8 1 4892 206 114 4258 1.8 1 4064 194 115 3643 1.8 1 3483 160 116 2968 1.8 1 2837 131 117 2599 1.8 1 2488 111 118 2126 1.8 1 2013 113 119 1795 1.8 1 1729 66 120 4379 1.8 1 4247 132 121 1386 1.8 1 1321 65 122 850 1.8 1 797 53 123 624 1.8 1 593 31 124 419 1.8 1 377 42 125 324 1.8 1 286 38 126 291 1.8 1 263 28 127 188 1.8 1 170 18 128 136 1.8 1 116 20 129 118 1.8 1 104 14 130 124 1.8 1 107 17 131 97 1.8 1 80 17 132 70 1.8 1 61 9 133 100 1.8 1 86 14 134 67 1.8 1 55 12 135 77 1.8 1 66 11 136 58 1.8 1 47 11 137 65 1.8 1 50 15 138 47 1.8 1 36 11 139 80 1.8 1 70 10 140 52 1.8 1 37 15 141 65 1.8 1 47 18 142 35 1.8 1 28 7 143 47 1.8 1 30 17 144 37 1.8 1 21 16 145 56 1.8 1 33 23 146 45 1.8 1 22 23 147 47 1.8 1 22 25 148 41 1.8 1 23 18 149 58 1.8 1 38 20 150 202 1.8 1 10 192 151 65947 1.8 1 171 65776 RUN STATISTICS FOR INPUT FILE: ERR10684014_1.fastq.gz ============================================= 124042011 sequences processed in total