SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684013_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 130315). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684013_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 2320.094 s (18.228 µs/read; 3.29 M reads/minute). === Summary === Total reads processed: 127,279,130 Reads with adapters: 62,092,246 (48.8%) Reads written (passing filters): 127,279,130 (100.0%) Total basepairs processed: 19,219,148,630 bp Quality-trimmed: 35,316,682 bp (0.2%) Total written (filtered): 18,341,272,380 bp (95.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 62092246 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.0% C: 0.4% G: 14.0% T: 49.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 34350296 31819782.5 0 34350296 2 5158543 7954945.6 0 5158543 3 2249403 1988736.4 0 2249403 4 1102073 497184.1 0 1102073 5 344585 124296.0 0 344585 6 290414 31074.0 0 290414 7 273701 7768.5 0 273701 8 308693 1942.1 0 308693 9 309891 485.5 0 308728 1163 10 290793 121.4 1 268091 22702 11 326175 30.3 1 288354 37821 12 335775 7.6 1 301213 34562 13 280727 1.9 1 257771 22956 14 318529 1.9 1 284254 34275 15 302869 1.9 1 276716 26153 16 311510 1.9 1 277793 33717 17 348683 1.9 1 308294 40389 18 363707 1.9 1 335868 27839 19 202889 1.9 1 186149 16740 20 291255 1.9 1 268255 23000 21 304110 1.9 1 276762 27348 22 278051 1.9 1 258046 20005 23 312127 1.9 1 285046 27081 24 303547 1.9 1 268686 34861 25 294366 1.9 1 272000 22366 26 254285 1.9 1 236828 17457 27 303703 1.9 1 278489 25214 28 272838 1.9 1 253771 19067 29 289586 1.9 1 261550 28036 30 340490 1.9 1 317952 22538 31 189698 1.9 1 176684 13014 32 274340 1.9 1 255794 18546 33 269012 1.9 1 245863 23149 34 287245 1.9 1 263189 24056 35 331998 1.9 1 309794 22204 36 177506 1.9 1 164404 13102 37 271346 1.9 1 253184 18162 38 245712 1.9 1 230964 14748 39 254061 1.9 1 233043 21018 40 262093 1.9 1 243630 18463 41 270898 1.9 1 253685 17213 42 260251 1.9 1 246015 14236 43 245187 1.9 1 221447 23740 44 241296 1.9 1 219605 21691 45 390225 1.9 1 370838 19387 46 86906 1.9 1 78866 8040 47 200365 1.9 1 190985 9380 48 281377 1.9 1 265212 16165 49 190971 1.9 1 180031 10940 50 218687 1.9 1 204006 14681 51 255612 1.9 1 234528 21084 52 237279 1.9 1 223842 13437 53 186852 1.9 1 178946 7906 54 207289 1.9 1 196098 11191 55 362895 1.9 1 346951 15944 56 135883 1.9 1 127443 8440 57 272807 1.9 1 263579 9228 58 241225 1.9 1 231752 9473 59 157117 1.9 1 148307 8810 60 128778 1.9 1 124853 3925 61 78059 1.9 1 73569 4490 62 267001 1.9 1 256193 10808 63 198815 1.9 1 193562 5253 64 112105 1.9 1 107760 4345 65 79890 1.9 1 75327 4563 66 217658 1.9 1 209971 7687 67 171870 1.9 1 165141 6729 68 217912 1.9 1 208982 8930 69 160672 1.9 1 153114 7558 70 159088 1.9 1 150668 8420 71 28607 1.9 1 26907 1700 72 49255 1.9 1 47393 1862 73 97819 1.9 1 94420 3399 74 115671 1.9 1 111630 4041 75 120116 1.9 1 115562 4554 76 119529 1.9 1 115025 4504 77 116319 1.9 1 111853 4466 78 114277 1.9 1 109741 4536 79 110289 1.9 1 105966 4323 80 105744 1.9 1 101575 4169 81 101054 1.9 1 97123 3931 82 98467 1.9 1 94639 3828 83 93894 1.9 1 90211 3683 84 90147 1.9 1 86596 3551 85 86446 1.9 1 83149 3297 86 82610 1.9 1 79483 3127 87 78716 1.9 1 75747 2969 88 75339 1.9 1 72552 2787 89 71618 1.9 1 69111 2507 90 67727 1.9 1 65209 2518 91 64176 1.9 1 61744 2432 92 61368 1.9 1 59145 2223 93 57167 1.9 1 55168 1999 94 54210 1.9 1 52289 1921 95 51026 1.9 1 49291 1735 96 47636 1.9 1 45964 1672 97 44335 1.9 1 42806 1529 98 40910 1.9 1 39567 1343 99 38130 1.9 1 36912 1218 100 35379 1.9 1 34167 1212 101 32305 1.9 1 31241 1064 102 29358 1.9 1 28371 987 103 27231 1.9 1 26344 887 104 24025 1.9 1 23232 793 105 21361 1.9 1 20676 685 106 18785 1.9 1 18202 583 107 16703 1.9 1 16118 585 108 14635 1.9 1 14186 449 109 12612 1.9 1 12200 412 110 10892 1.9 1 10552 340 111 9307 1.9 1 9013 294 112 7768 1.9 1 7512 256 113 6395 1.9 1 6204 191 114 5089 1.9 1 4916 173 115 4096 1.9 1 3968 128 116 3364 1.9 1 3239 125 117 2716 1.9 1 2611 105 118 1971 1.9 1 1900 71 119 1623 1.9 1 1546 77 120 2069 1.9 1 2017 52 121 1010 1.9 1 961 49 122 689 1.9 1 655 34 123 495 1.9 1 453 42 124 337 1.9 1 312 25 125 238 1.9 1 217 21 126 200 1.9 1 182 18 127 149 1.9 1 134 15 128 123 1.9 1 115 8 129 114 1.9 1 104 10 130 124 1.9 1 112 12 131 110 1.9 1 99 11 132 60 1.9 1 53 7 133 116 1.9 1 108 8 134 59 1.9 1 49 10 135 72 1.9 1 57 15 136 64 1.9 1 47 17 137 73 1.9 1 64 9 138 55 1.9 1 49 6 139 51 1.9 1 43 8 140 47 1.9 1 38 9 141 68 1.9 1 51 17 142 30 1.9 1 24 6 143 32 1.9 1 19 13 144 24 1.9 1 18 6 145 25 1.9 1 15 10 146 18 1.9 1 9 9 147 31 1.9 1 22 9 148 39 1.9 1 25 14 149 44 1.9 1 24 20 150 48 1.9 1 14 34 151 5820 1.9 1 30 5790 RUN STATISTICS FOR INPUT FILE: ERR10684013_2.fastq.gz ============================================= 127279130 sequences processed in total