SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684012_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 128151). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684012_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2717.227 s (16.727 µs/read; 3.59 M reads/minute). === Summary === Total reads processed: 162,448,263 Reads with adapters: 79,148,195 (48.7%) Reads written (passing filters): 162,448,263 (100.0%) Total basepairs processed: 24,529,687,713 bp Quality-trimmed: 39,509,023 bp (0.2%) Total written (filtered): 23,400,276,817 bp (95.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 79148195 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 35.9% C: 0.5% G: 14.1% T: 49.4% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 43730899 40612065.8 0 43730899 2 6540102 10153016.4 0 6540102 3 2876415 2538254.1 0 2876415 4 1420931 634563.5 0 1420931 5 454390 158640.9 0 454390 6 377239 39660.2 0 377239 7 352192 9915.1 0 352192 8 404186 2478.8 0 404186 9 404757 619.7 0 401844 2913 10 377898 154.9 1 344205 33693 11 426486 38.7 1 371740 54746 12 435325 9.7 1 384781 50544 13 375286 2.4 1 339204 36082 14 409441 2.4 1 360796 48645 15 404693 2.4 1 363661 41032 16 400773 2.4 1 352812 47961 17 449838 2.4 1 392034 57804 18 480458 2.4 1 439112 41346 19 259258 2.4 1 233814 25444 20 381196 2.4 1 345934 35262 21 389430 2.4 1 350354 39076 22 358013 2.4 1 327436 30577 23 394093 2.4 1 355275 38818 24 392003 2.4 1 343488 48515 25 381218 2.4 1 347072 34146 26 332863 2.4 1 304741 28122 27 387597 2.4 1 350794 36803 28 348438 2.4 1 319901 28537 29 372666 2.4 1 331620 41046 30 437663 2.4 1 404243 33420 31 238315 2.4 1 218675 19640 32 348981 2.4 1 320674 28307 33 331570 2.4 1 302094 29476 34 355997 2.4 1 323120 32877 35 324317 2.4 1 298217 26100 36 325752 2.4 1 297182 28570 37 358266 2.4 1 323692 34574 38 297682 2.4 1 277322 20360 39 340050 2.4 1 311259 28791 40 416107 2.4 1 384716 31391 41 268439 2.4 1 241200 27239 42 264312 2.4 1 247181 17131 43 363277 2.4 1 322937 40340 44 272789 2.4 1 245860 26929 45 474603 2.4 1 448341 26262 46 101116 2.4 1 89908 11208 47 260520 2.4 1 244524 15996 48 327153 2.4 1 305365 21788 49 229462 2.4 1 214245 15217 50 280019 2.4 1 257819 22200 51 286646 2.4 1 261056 25590 52 271926 2.4 1 252561 19365 53 249451 2.4 1 234611 14840 54 257232 2.4 1 238499 18733 55 378441 2.4 1 356626 21815 56 157370 2.4 1 144341 13029 57 307493 2.4 1 290935 16558 58 334191 2.4 1 317540 16651 59 152696 2.4 1 140531 12165 60 203319 2.4 1 195371 7948 61 121632 2.4 1 112410 9222 62 288255 2.4 1 271653 16602 63 253016 2.4 1 243391 9625 64 178444 2.4 1 169960 8484 65 103082 2.4 1 95142 7940 66 241960 2.4 1 229749 12211 67 244449 2.4 1 231664 12785 68 293821 2.4 1 279058 14763 69 195179 2.4 1 183631 11548 70 183782 2.4 1 172945 10837 71 40532 2.4 1 37749 2783 72 65506 2.4 1 61840 3666 73 123537 2.4 1 117751 5786 74 141566 2.4 1 134896 6670 75 146149 2.4 1 138818 7331 76 144026 2.4 1 136710 7316 77 140961 2.4 1 133616 7345 78 137546 2.4 1 130634 6912 79 133689 2.4 1 126935 6754 80 129700 2.4 1 123146 6554 81 125200 2.4 1 118982 6218 82 120394 2.4 1 114399 5995 83 116254 2.4 1 110590 5664 84 111832 2.4 1 106136 5696 85 107888 2.4 1 102750 5138 86 103368 2.4 1 98492 4876 87 98494 2.4 1 93863 4631 88 95243 2.4 1 90752 4491 89 91115 2.4 1 86783 4332 90 86781 2.4 1 82767 4014 91 82909 2.4 1 78947 3962 92 79299 2.4 1 75709 3590 93 75906 2.4 1 72471 3435 94 71638 2.4 1 68267 3371 95 68351 2.4 1 65225 3126 96 64892 2.4 1 61984 2908 97 61052 2.4 1 58265 2787 98 57936 2.4 1 55387 2549 99 54551 2.4 1 52117 2434 100 51582 2.4 1 49343 2239 101 48456 2.4 1 46376 2080 102 44901 2.4 1 42832 2069 103 41500 2.4 1 39749 1751 104 38395 2.4 1 36746 1649 105 35275 2.4 1 33741 1534 106 32136 2.4 1 30783 1353 107 29801 2.4 1 28615 1186 108 26666 2.4 1 25580 1086 109 24205 2.4 1 23219 986 110 21493 2.4 1 20611 882 111 19119 2.4 1 18333 786 112 16720 2.4 1 16019 701 113 14384 2.4 1 13836 548 114 12014 2.4 1 11543 471 115 10494 2.4 1 10064 430 116 8774 2.4 1 8419 355 117 7380 2.4 1 7077 303 118 5847 2.4 1 5604 243 119 5070 2.4 1 4834 236 120 17402 2.4 1 16852 550 121 4844 2.4 1 4644 200 122 2270 2.4 1 2187 83 123 1590 2.4 1 1515 75 124 978 2.4 1 913 65 125 627 2.4 1 589 38 126 506 2.4 1 473 33 127 326 2.4 1 311 15 128 238 2.4 1 219 19 129 186 2.4 1 163 23 130 158 2.4 1 141 17 131 115 2.4 1 97 18 132 94 2.4 1 71 23 133 107 2.4 1 94 13 134 68 2.4 1 58 10 135 126 2.4 1 110 16 136 51 2.4 1 42 9 137 72 2.4 1 61 11 138 48 2.4 1 41 7 139 81 2.4 1 72 9 140 62 2.4 1 44 18 141 74 2.4 1 58 16 142 39 2.4 1 23 16 143 55 2.4 1 29 26 144 36 2.4 1 22 14 145 71 2.4 1 47 24 146 38 2.4 1 17 21 147 56 2.4 1 21 35 148 54 2.4 1 24 30 149 70 2.4 1 30 40 150 292 2.4 1 9 283 151 110075 2.4 1 215 109860 RUN STATISTICS FOR INPUT FILE: ERR10684012_1.fastq.gz ============================================= 162448263 sequences processed in total