Bismark report for: ERR10684011_val_1.fq.gz and ERR10684011_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 71554643 Number of paired-end alignments with a unique best hit: 33409027 Mapping efficiency: 46.7% Sequence pairs with no alignments under any condition: 29573498 Sequence pairs did not map uniquely: 8572118 Sequence pairs which were discarded because genomic sequence could not be extracted: 34 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 16700488 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 16708505 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1785842963 Total methylated C's in CpG context: 59430352 Total methylated C's in CHG context: 29264757 Total methylated C's in CHH context: 114757741 Total methylated C's in Unknown context: 590187 Total unmethylated C's in CpG context: 184269283 Total unmethylated C's in CHG context: 301143823 Total unmethylated C's in CHH context: 1096977007 Total unmethylated C's in Unknown context: 5828602 C methylated in CpG context: 24.4% C methylated in CHG context: 8.9% C methylated in CHH context: 9.5% C methylated in Unknown context (CN or CHN): 9.2% Bismark completed in 0d 3h 56m 58s