SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684011_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 106087). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684011_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1217.757 s (16.993 µs/read; 3.53 M reads/minute). === Summary === Total reads processed: 71,661,379 Reads with adapters: 33,634,747 (46.9%) Reads written (passing filters): 71,661,379 (100.0%) Total basepairs processed: 10,820,868,229 bp Quality-trimmed: 35,309,091 bp (0.3%) Total written (filtered): 10,397,900,517 bp (96.1%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 33634747 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 34.3% C: 2.0% G: 16.0% T: 47.4% none/other: 0.2% Overview of removed sequences length count expect max.err error counts 1 18967970 17915344.8 0 18967970 2 3211532 4478836.2 0 3211532 3 1353155 1119709.0 0 1353155 4 601028 279927.3 0 601028 5 202481 69981.8 0 202481 6 174555 17495.5 0 174555 7 167915 4373.9 0 167915 8 186927 1093.5 0 186927 9 175252 273.4 0 174385 867 10 173067 68.3 1 157088 15979 11 185791 17.1 1 163327 22464 12 192000 4.3 1 170391 21609 13 168578 1.1 1 152079 16499 14 179262 1.1 1 158803 20459 15 178397 1.1 1 160438 17959 16 174549 1.1 1 154074 20475 17 195348 1.1 1 170847 24501 18 210205 1.1 1 191053 19152 19 111143 1.1 1 101012 10131 20 161431 1.1 1 146851 14580 21 162945 1.1 1 147076 15869 22 152483 1.1 1 138955 13528 23 168451 1.1 1 151597 16854 24 161786 1.1 1 141276 20510 25 161942 1.1 1 147761 14181 26 131567 1.1 1 121136 10431 27 160408 1.1 1 144566 15842 28 148354 1.1 1 135160 13194 29 152708 1.1 1 135475 17233 30 179197 1.1 1 164502 14695 31 94368 1.1 1 86493 7875 32 136307 1.1 1 125021 11286 33 140390 1.1 1 125193 15197 34 155765 1.1 1 140405 15360 35 109572 1.1 1 100778 8794 36 143512 1.1 1 129791 13721 37 161548 1.1 1 148294 13254 38 85631 1.1 1 78615 7016 39 122565 1.1 1 113017 9548 40 121538 1.1 1 111652 9886 41 121346 1.1 1 111245 10101 42 132960 1.1 1 121364 11596 43 151066 1.1 1 132036 19030 44 129165 1.1 1 113692 15473 45 213540 1.1 1 198786 14754 46 53614 1.1 1 46668 6946 47 107439 1.1 1 98900 8539 48 714036 1.1 1 681305 32731 49 168653 1.1 1 157448 11205 50 59160 1.1 1 54476 4684 51 176770 1.1 1 166845 9925 52 130189 1.1 1 122564 7625 53 14536 1.1 1 12931 1605 54 83997 1.1 1 80715 3282 55 83678 1.1 1 79798 3880 56 24732 1.1 1 22569 2163 57 17065 1.1 1 16032 1033 58 9821 1.1 1 8544 1277 59 21243 1.1 1 19550 1693 60 6415 1.1 1 5437 978 61 9845 1.1 1 8672 1173 62 72127 1.1 1 68545 3582 63 10665 1.1 1 9790 875 64 12153 1.1 1 11043 1110 65 15933 1.1 1 14557 1376 66 65872 1.1 1 62566 3306 67 14357 1.1 1 13231 1126 68 37717 1.1 1 35548 2169 69 42706 1.1 1 40204 2502 70 56739 1.1 1 53705 3034 71 11765 1.1 1 10549 1216 72 10194 1.1 1 9336 858 73 24262 1.1 1 22992 1270 74 36820 1.1 1 35016 1804 75 42768 1.1 1 40709 2059 76 44200 1.1 1 41927 2273 77 43568 1.1 1 41367 2201 78 42930 1.1 1 40776 2154 79 41294 1.1 1 39193 2101 80 39765 1.1 1 37723 2042 81 38014 1.1 1 36109 1905 82 36875 1.1 1 34972 1903 83 35033 1.1 1 33302 1731 84 33517 1.1 1 31756 1761 85 31843 1.1 1 30280 1563 86 30414 1.1 1 28939 1475 87 29149 1.1 1 27614 1535 88 28049 1.1 1 26812 1237 89 26247 1.1 1 24989 1258 90 25017 1.1 1 23848 1169 91 23558 1.1 1 22427 1131 92 22368 1.1 1 21344 1024 93 21166 1.1 1 20181 985 94 19987 1.1 1 19052 935 95 18858 1.1 1 18021 837 96 17833 1.1 1 17070 763 97 16706 1.1 1 15899 807 98 15498 1.1 1 14753 745 99 14638 1.1 1 14024 614 100 13832 1.1 1 13271 561 101 12763 1.1 1 12169 594 102 11835 1.1 1 11359 476 103 10992 1.1 1 10514 478 104 9924 1.1 1 9505 419 105 9240 1.1 1 8848 392 106 8351 1.1 1 7989 362 107 7614 1.1 1 7302 312 108 6837 1.1 1 6563 274 109 6220 1.1 1 5940 280 110 5627 1.1 1 5383 244 111 5049 1.1 1 4827 222 112 4482 1.1 1 4301 181 113 4021 1.1 1 3863 158 114 3434 1.1 1 3272 162 115 3061 1.1 1 2931 130 116 2633 1.1 1 2511 122 117 2328 1.1 1 2206 122 118 2027 1.1 1 1924 103 119 1741 1.1 1 1644 97 120 10311 1.1 1 10002 309 121 2136 1.1 1 2057 79 122 1048 1.1 1 1002 46 123 784 1.1 1 730 54 124 618 1.1 1 584 34 125 477 1.1 1 435 42 126 405 1.1 1 375 30 127 323 1.1 1 284 39 128 225 1.1 1 211 14 129 202 1.1 1 187 15 130 199 1.1 1 174 25 131 236 1.1 1 218 18 132 158 1.1 1 137 21 133 178 1.1 1 162 16 134 144 1.1 1 130 14 135 171 1.1 1 151 20 136 144 1.1 1 124 20 137 182 1.1 1 158 24 138 110 1.1 1 95 15 139 149 1.1 1 135 14 140 114 1.1 1 97 17 141 139 1.1 1 117 22 142 98 1.1 1 77 21 143 126 1.1 1 93 33 144 82 1.1 1 54 28 145 158 1.1 1 118 40 146 125 1.1 1 97 28 147 120 1.1 1 82 38 148 104 1.1 1 69 35 149 148 1.1 1 119 29 150 287 1.1 1 64 223 151 81537 1.1 1 215 81322 RUN STATISTICS FOR INPUT FILE: ERR10684011_1.fastq.gz ============================================= 71661379 sequences processed in total