SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684010_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 103381). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684010_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1798.358 s (16.968 µs/read; 3.54 M reads/minute). === Summary === Total reads processed: 105,987,586 Reads with adapters: 49,987,073 (47.2%) Reads written (passing filters): 105,987,586 (100.0%) Total basepairs processed: 16,004,125,486 bp Quality-trimmed: 29,651,993 bp (0.2%) Total written (filtered): 15,404,812,037 bp (96.3%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 49987073 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 35.9% C: 0.6% G: 14.2% T: 49.1% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 29209156 26496896.5 0 29209156 2 4469137 6624224.1 0 4469137 3 1936512 1656056.0 0 1936512 4 921212 414014.0 0 921212 5 279384 103503.5 0 279384 6 236172 25875.9 0 236172 7 219841 6469.0 0 219841 8 248773 1617.2 0 248773 9 247939 404.3 0 245853 2086 10 232506 101.1 1 210842 21664 11 259829 25.3 1 226340 33489 12 265297 6.3 1 233546 31751 13 231662 1.6 1 208520 23142 14 244197 1.6 1 215099 29098 15 246214 1.6 1 220344 25870 16 239918 1.6 1 210420 29498 17 266315 1.6 1 231647 34668 18 297679 1.6 1 271150 26529 19 144946 1.6 1 129972 14974 20 229732 1.6 1 208261 21471 21 223543 1.6 1 201315 22228 22 215625 1.6 1 195703 19922 23 232401 1.6 1 208792 23609 24 230952 1.6 1 201943 29009 25 223879 1.6 1 203188 20691 26 192832 1.6 1 176119 16713 27 225790 1.6 1 204097 21693 28 201544 1.6 1 184486 17058 29 215643 1.6 1 191576 24067 30 247663 1.6 1 228491 19172 31 139541 1.6 1 126764 12777 32 197839 1.6 1 181458 16381 33 192346 1.6 1 174236 18110 34 197008 1.6 1 178200 18808 35 194134 1.6 1 174985 19149 36 194166 1.6 1 178806 15360 37 171637 1.6 1 157216 14421 38 196306 1.6 1 178701 17605 39 208567 1.6 1 189372 19195 40 168825 1.6 1 157012 11813 41 140143 1.6 1 130250 9893 42 165980 1.6 1 151434 14546 43 217139 1.6 1 191806 25333 44 157001 1.6 1 139968 17033 45 239834 1.6 1 226952 12882 46 65658 1.6 1 58455 7203 47 137831 1.6 1 128899 8932 48 188676 1.6 1 175518 13158 49 126274 1.6 1 117189 9085 50 147438 1.6 1 134863 12575 51 166799 1.6 1 151085 15714 52 152450 1.6 1 141783 10667 53 117056 1.6 1 110666 6390 54 138377 1.6 1 128088 10289 55 234721 1.6 1 223185 11536 56 85694 1.6 1 78470 7224 57 172360 1.6 1 165381 6979 58 133094 1.6 1 126730 6364 59 96077 1.6 1 89951 6126 60 73038 1.6 1 70295 2743 61 45660 1.6 1 41205 4455 62 159632 1.6 1 151832 7800 63 116805 1.6 1 113186 3619 64 58696 1.6 1 55711 2985 65 48680 1.6 1 44545 4135 66 127877 1.6 1 122215 5662 67 95100 1.6 1 90112 4988 68 119396 1.6 1 113591 5805 69 91963 1.6 1 86772 5191 70 90013 1.6 1 85016 4997 71 16962 1.6 1 15572 1390 72 28455 1.6 1 26816 1639 73 55278 1.6 1 52666 2612 74 65627 1.6 1 62572 3055 75 67847 1.6 1 64595 3252 76 67154 1.6 1 63798 3356 77 65271 1.6 1 62004 3267 78 62935 1.6 1 59713 3222 79 60579 1.6 1 57584 2995 80 58923 1.6 1 56122 2801 81 56925 1.6 1 54109 2816 82 54361 1.6 1 51815 2546 83 52280 1.6 1 49738 2542 84 50139 1.6 1 47675 2464 85 47173 1.6 1 44890 2283 86 45859 1.6 1 43728 2131 87 44149 1.6 1 42041 2108 88 41917 1.6 1 39997 1920 89 40229 1.6 1 38422 1807 90 38333 1.6 1 36624 1709 91 36294 1.6 1 34598 1696 92 34709 1.6 1 33233 1476 93 32894 1.6 1 31400 1494 94 30868 1.6 1 29452 1416 95 29385 1.6 1 28136 1249 96 28035 1.6 1 26792 1243 97 26131 1.6 1 24994 1137 98 24626 1.6 1 23550 1076 99 23471 1.6 1 22463 1008 100 22474 1.6 1 21499 975 101 20845 1.6 1 20027 818 102 19081 1.6 1 18296 785 103 17944 1.6 1 17176 768 104 16484 1.6 1 15795 689 105 15075 1.6 1 14463 612 106 13613 1.6 1 13045 568 107 12732 1.6 1 12261 471 108 11513 1.6 1 11002 511 109 10041 1.6 1 9649 392 110 9332 1.6 1 8938 394 111 8296 1.6 1 7974 322 112 7230 1.6 1 6937 293 113 6268 1.6 1 6005 263 114 5616 1.6 1 5396 220 115 4572 1.6 1 4377 195 116 3931 1.6 1 3764 167 117 3334 1.6 1 3191 143 118 2763 1.6 1 2644 119 119 2340 1.6 1 2188 152 120 7781 1.6 1 7554 227 121 2223 1.6 1 2118 105 122 1178 1.6 1 1137 41 123 938 1.6 1 876 62 124 646 1.6 1 601 45 125 449 1.6 1 416 33 126 432 1.6 1 395 37 127 308 1.6 1 287 21 128 202 1.6 1 177 25 129 161 1.6 1 142 19 130 152 1.6 1 138 14 131 162 1.6 1 143 19 132 112 1.6 1 98 14 133 128 1.6 1 117 11 134 100 1.6 1 84 16 135 137 1.6 1 118 19 136 84 1.6 1 70 14 137 117 1.6 1 96 21 138 75 1.6 1 61 14 139 104 1.6 1 87 17 140 64 1.6 1 50 14 141 86 1.6 1 64 22 142 46 1.6 1 36 10 143 70 1.6 1 58 12 144 55 1.6 1 37 18 145 51 1.6 1 35 16 146 54 1.6 1 45 9 147 48 1.6 1 32 16 148 57 1.6 1 31 26 149 47 1.6 1 29 18 150 220 1.6 1 25 195 151 64369 1.6 1 150 64219 RUN STATISTICS FOR INPUT FILE: ERR10684010_1.fastq.gz ============================================= 105987586 sequences processed in total