SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684009_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 121147). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684009_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 2226.735 s (16.937 µs/read; 3.54 M reads/minute). === Summary === Total reads processed: 131,474,544 Reads with adapters: 63,490,865 (48.3%) Reads written (passing filters): 131,474,544 (100.0%) Total basepairs processed: 19,852,656,144 bp Quality-trimmed: 31,808,103 bp (0.2%) Total written (filtered): 19,003,404,909 bp (95.7%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 63490865 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.0% C: 0.4% G: 14.1% T: 49.5% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 35781830 32868636.0 0 35781830 2 5342250 8217159.0 0 5342250 3 2347971 2054289.8 0 2347971 4 1142301 513572.4 0 1142301 5 357105 128393.1 0 357105 6 298914 32098.3 0 298914 7 279052 8024.6 0 279052 8 318469 2006.1 0 318469 9 321204 501.5 0 318540 2664 10 298606 125.4 1 272567 26039 11 337684 31.3 1 294846 42838 12 346511 7.8 1 307433 39078 13 289229 2.0 1 262428 26801 14 326364 2.0 1 287106 39258 15 311747 2.0 1 281424 30323 16 320329 2.0 1 281929 38400 17 357180 2.0 1 312116 45064 18 375067 2.0 1 343598 31469 19 204726 2.0 1 184459 20267 20 301866 2.0 1 273914 27952 21 305804 2.0 1 275357 30447 22 283729 2.0 1 259806 23923 23 316791 2.0 1 286228 30563 24 306347 2.0 1 267988 38359 25 301086 2.0 1 275181 25905 26 256467 2.0 1 235444 21023 27 305730 2.0 1 276827 28903 28 276445 2.0 1 253446 22999 29 295042 2.0 1 262624 32418 30 338818 2.0 1 313746 25072 31 193544 2.0 1 176900 16644 32 277915 2.0 1 256144 21771 33 264624 2.0 1 239101 25523 34 281290 2.0 1 255729 25561 35 309680 2.0 1 287068 22612 36 192364 2.0 1 178180 14184 37 265331 2.0 1 238615 26716 38 257693 2.0 1 236056 21637 39 260779 2.0 1 237835 22944 40 234534 2.0 1 220312 14222 41 243569 2.0 1 221366 22203 42 265199 2.0 1 244469 20730 43 341837 2.0 1 309370 32467 44 153398 2.0 1 137021 16377 45 380549 2.0 1 359668 20881 46 77926 2.0 1 69369 8557 47 189903 2.0 1 178276 11627 48 268947 2.0 1 252225 16722 49 165247 2.0 1 154441 10806 50 219109 2.0 1 201961 17148 51 213121 2.0 1 194862 18259 52 207383 2.0 1 193229 14154 53 193682 2.0 1 182153 11529 54 201992 2.0 1 186763 15229 55 292036 2.0 1 275925 16111 56 117940 2.0 1 108141 9799 57 239158 2.0 1 226568 12590 58 252578 2.0 1 240335 12243 59 114340 2.0 1 105186 9154 60 154952 2.0 1 148905 6047 61 93156 2.0 1 86079 7077 62 215446 2.0 1 203311 12135 63 192132 2.0 1 184780 7352 64 133943 2.0 1 127794 6149 65 77895 2.0 1 71681 6214 66 179150 2.0 1 170467 8683 67 181813 2.0 1 172105 9708 68 214019 2.0 1 203342 10677 69 148559 2.0 1 139767 8792 70 151339 2.0 1 142445 8894 71 29283 2.0 1 27184 2099 72 46590 2.0 1 43936 2654 73 89573 2.0 1 85449 4124 74 103736 2.0 1 98989 4747 75 107150 2.0 1 101840 5310 76 106478 2.0 1 101155 5323 77 103436 2.0 1 98151 5285 78 100130 2.0 1 95083 5047 79 97352 2.0 1 92524 4828 80 93726 2.0 1 88979 4747 81 90391 2.0 1 85831 4560 82 88044 2.0 1 83619 4425 83 83610 2.0 1 79512 4098 84 80311 2.0 1 76292 4019 85 76836 2.0 1 73058 3778 86 74082 2.0 1 70417 3665 87 71347 2.0 1 67966 3381 88 67525 2.0 1 64384 3141 89 64539 2.0 1 61517 3022 90 61476 2.0 1 58611 2865 91 58650 2.0 1 55839 2811 92 55358 2.0 1 52809 2549 93 52518 2.0 1 50118 2400 94 50174 2.0 1 47875 2299 95 47773 2.0 1 45578 2195 96 45012 2.0 1 42959 2053 97 42393 2.0 1 40461 1932 98 39587 2.0 1 37817 1770 99 37125 2.0 1 35535 1590 100 34650 2.0 1 33200 1450 101 32485 2.0 1 31052 1433 102 30392 2.0 1 29081 1311 103 27761 2.0 1 26539 1222 104 25276 2.0 1 24157 1119 105 23329 2.0 1 22369 960 106 20974 2.0 1 20100 874 107 19075 2.0 1 18310 765 108 16884 2.0 1 16201 683 109 15079 2.0 1 14422 657 110 13378 2.0 1 12784 594 111 11788 2.0 1 11322 466 112 10151 2.0 1 9702 449 113 8775 2.0 1 8433 342 114 7399 2.0 1 7082 317 115 6284 2.0 1 6017 267 116 5035 2.0 1 4831 204 117 4146 2.0 1 3983 163 118 3378 2.0 1 3244 134 119 2635 2.0 1 2531 104 120 3869 2.0 1 3732 137 121 1720 2.0 1 1645 75 122 1106 2.0 1 1059 47 123 784 2.0 1 733 51 124 490 2.0 1 458 32 125 326 2.0 1 306 20 126 264 2.0 1 251 13 127 159 2.0 1 134 25 128 131 2.0 1 110 21 129 72 2.0 1 64 8 130 69 2.0 1 57 12 131 63 2.0 1 52 11 132 63 2.0 1 44 19 133 53 2.0 1 44 9 134 42 2.0 1 36 6 135 45 2.0 1 37 8 136 26 2.0 1 21 5 137 49 2.0 1 43 6 138 33 2.0 1 28 5 139 36 2.0 1 29 7 140 29 2.0 1 21 8 141 42 2.0 1 30 12 142 33 2.0 1 25 8 143 20 2.0 1 12 8 144 22 2.0 1 9 13 145 36 2.0 1 20 16 146 20 2.0 1 2 18 147 24 2.0 1 15 9 148 23 2.0 1 6 17 149 44 2.0 1 12 32 150 121 2.0 1 7 114 151 35236 2.0 1 52 35184 RUN STATISTICS FOR INPUT FILE: ERR10684009_1.fastq.gz ============================================= 131474544 sequences processed in total