SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684008_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 225983). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684008_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1447.277 s (15.579 µs/read; 3.85 M reads/minute). === Summary === Total reads processed: 92,899,213 Reads with adapters: 54,970,005 (59.2%) Reads written (passing filters): 92,899,213 (100.0%) Total basepairs processed: 14,027,781,163 bp Quality-trimmed: 23,514,924 bp (0.2%) Total written (filtered): 13,070,583,865 bp (93.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 54970005 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.7% C: 1.3% G: 17.1% T: 44.8% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 19966095 23224803.2 0 19966095 2 3686855 5806200.8 0 3686855 3 1948723 1451550.2 0 1948723 4 1217144 362887.6 0 1217144 5 787066 90721.9 0 787066 6 700386 22680.5 0 700386 7 724564 5670.1 0 724564 8 762124 1417.5 0 762124 9 733382 354.4 0 729515 3867 10 712148 88.6 1 673507 38641 11 710378 22.1 1 660616 49762 12 709390 5.5 1 665913 43477 13 602913 1.4 1 568698 34215 14 632210 1.4 1 587823 44387 15 616923 1.4 1 581564 35359 16 628104 1.4 1 581368 46736 17 690205 1.4 1 638681 51524 18 725839 1.4 1 686192 39647 19 526758 1.4 1 496657 30101 20 598481 1.4 1 564806 33675 21 563809 1.4 1 528452 35357 22 516582 1.4 1 484274 32308 23 531933 1.4 1 499070 32863 24 485173 1.4 1 445784 39389 25 491052 1.4 1 461518 29534 26 447652 1.4 1 425232 22420 27 515656 1.4 1 484693 30963 28 522595 1.4 1 494291 28304 29 545790 1.4 1 507797 37993 30 571830 1.4 1 544533 27297 31 409074 1.4 1 389443 19631 32 437665 1.4 1 416143 21522 33 390482 1.4 1 368789 21693 34 377585 1.4 1 359025 18560 35 416035 1.4 1 387631 28404 36 316334 1.4 1 301496 14838 37 358935 1.4 1 342322 16613 38 387999 1.4 1 369336 18663 39 370361 1.4 1 354144 16217 40 385876 1.4 1 364835 21041 41 363822 1.4 1 343891 19931 42 330077 1.4 1 312444 17633 43 345633 1.4 1 323576 22057 44 218764 1.4 1 206064 12700 45 425269 1.4 1 410261 15008 46 87630 1.4 1 81048 6582 47 255248 1.4 1 243171 12077 48 326388 1.4 1 310803 15585 49 243210 1.4 1 232945 10265 50 268891 1.4 1 255107 13784 51 273297 1.4 1 258796 14501 52 228922 1.4 1 219332 9590 53 206828 1.4 1 197863 8965 54 230367 1.4 1 217059 13308 55 266836 1.4 1 256528 10308 56 123146 1.4 1 116472 6674 57 241408 1.4 1 232685 8723 58 215277 1.4 1 207936 7341 59 167311 1.4 1 159799 7512 60 206303 1.4 1 200643 5660 61 115845 1.4 1 110214 5631 62 194646 1.4 1 186372 8274 63 196260 1.4 1 190524 5736 64 107359 1.4 1 103466 3893 65 91493 1.4 1 86944 4549 66 150851 1.4 1 145283 5568 67 129762 1.4 1 124733 5029 68 164158 1.4 1 158021 6137 69 151448 1.4 1 143951 7497 70 242694 1.4 1 234465 8229 71 22283 1.4 1 20640 1643 72 38745 1.4 1 36642 2103 73 80126 1.4 1 76889 3237 74 87750 1.4 1 84316 3434 75 85067 1.4 1 81834 3233 76 81829 1.4 1 78454 3375 77 77800 1.4 1 74605 3195 78 76116 1.4 1 73024 3092 79 75105 1.4 1 72108 2997 80 75911 1.4 1 72859 3052 81 75970 1.4 1 72909 3061 82 69355 1.4 1 66538 2817 83 61321 1.4 1 58789 2532 84 55400 1.4 1 53223 2177 85 52078 1.4 1 49942 2136 86 47518 1.4 1 45592 1926 87 44771 1.4 1 42980 1791 88 42166 1.4 1 40554 1612 89 40841 1.4 1 39257 1584 90 39943 1.4 1 38508 1435 91 38760 1.4 1 37304 1456 92 36313 1.4 1 34982 1331 93 33025 1.4 1 31866 1159 94 29310 1.4 1 28189 1121 95 26264 1.4 1 25334 930 96 23992 1.4 1 23057 935 97 21950 1.4 1 21122 828 98 20377 1.4 1 19657 720 99 19159 1.4 1 18446 713 100 18184 1.4 1 17553 631 101 17374 1.4 1 16760 614 102 16104 1.4 1 15493 611 103 14807 1.4 1 14326 481 104 12852 1.4 1 12391 461 105 11313 1.4 1 10916 397 106 10341 1.4 1 9948 393 107 8887 1.4 1 8590 297 108 7983 1.4 1 7721 262 109 7283 1.4 1 7005 278 110 6609 1.4 1 6393 216 111 5603 1.4 1 5389 214 112 5079 1.4 1 4909 170 113 4422 1.4 1 4254 168 114 3672 1.4 1 3538 134 115 3084 1.4 1 2961 123 116 2538 1.4 1 2442 96 117 2127 1.4 1 2040 87 118 1733 1.4 1 1676 57 119 1379 1.4 1 1311 68 120 1919 1.4 1 1845 74 121 1006 1.4 1 955 51 122 724 1.4 1 696 28 123 456 1.4 1 420 36 124 363 1.4 1 336 27 125 262 1.4 1 231 31 126 197 1.4 1 180 17 127 147 1.4 1 130 17 128 102 1.4 1 88 14 129 80 1.4 1 60 20 130 60 1.4 1 42 18 131 61 1.4 1 46 15 132 47 1.4 1 37 10 133 68 1.4 1 48 20 134 42 1.4 1 23 19 135 39 1.4 1 25 14 136 27 1.4 1 15 12 137 52 1.4 1 24 28 138 42 1.4 1 23 19 139 38 1.4 1 26 12 140 29 1.4 1 14 15 141 35 1.4 1 18 17 142 30 1.4 1 16 14 143 33 1.4 1 10 23 144 26 1.4 1 9 17 145 33 1.4 1 17 16 146 41 1.4 1 12 29 147 41 1.4 1 17 24 148 32 1.4 1 14 18 149 48 1.4 1 15 33 150 63 1.4 1 10 53 151 31994 1.4 1 128 31866 RUN STATISTICS FOR INPUT FILE: ERR10684008_1.fastq.gz ============================================= 92899213 sequences processed in total