Bismark report for: ERR10684006_val_1.fq.gz and ERR10684006_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 84092043 Number of paired-end alignments with a unique best hit: 50537357 Mapping efficiency: 60.1% Sequence pairs with no alignments under any condition: 19649826 Sequence pairs did not map uniquely: 13904860 Sequence pairs which were discarded because genomic sequence could not be extracted: 11 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 25257967 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 25279379 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2474155584 Total methylated C's in CpG context: 47070518 Total methylated C's in CHG context: 12253714 Total methylated C's in CHH context: 48556616 Total methylated C's in Unknown context: 261253 Total unmethylated C's in CpG context: 282902460 Total unmethylated C's in CHG context: 433397168 Total unmethylated C's in CHH context: 1649975108 Total unmethylated C's in Unknown context: 6485556 C methylated in CpG context: 14.3% C methylated in CHG context: 2.7% C methylated in CHH context: 2.9% C methylated in Unknown context (CN or CHN): 3.9% Bismark completed in 0d 4h 9m 30s