SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684006_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 304222). Second best hit was smallRNA (count: 1) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684006_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 1647.493 s (19.587 µs/read; 3.06 M reads/minute). === Summary === Total reads processed: 84,111,724 Reads with adapters: 54,501,615 (64.8%) Reads written (passing filters): 84,111,724 (100.0%) Total basepairs processed: 12,700,870,324 bp Quality-trimmed: 36,274,028 bp (0.3%) Total written (filtered): 11,554,004,735 bp (91.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 54501615 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.6% C: 0.9% G: 17.8% T: 44.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 15756059 21027931.0 0 15756059 2 2987473 5256982.8 0 2987473 3 1667316 1314245.7 0 1667316 4 1109534 328561.4 0 1109534 5 777247 82140.4 0 777247 6 679227 20535.1 0 679227 7 703500 5133.8 0 703500 8 754515 1283.4 0 754515 9 725836 320.9 0 721891 3945 10 719791 80.2 1 675856 43935 11 737747 20.1 1 680406 57341 12 749898 5.0 1 697850 52048 13 650769 1.3 1 609324 41445 14 691483 1.3 1 638640 52843 15 689052 1.3 1 644237 44815 16 687482 1.3 1 632171 55311 17 750389 1.3 1 689410 60979 18 789630 1.3 1 740590 49040 19 584777 1.3 1 546814 37963 20 671488 1.3 1 628518 42970 21 652299 1.3 1 605829 46470 22 602268 1.3 1 559422 42846 23 631107 1.3 1 586142 44965 24 598188 1.3 1 544853 53335 25 599647 1.3 1 559629 40018 26 549295 1.3 1 518011 31284 27 625217 1.3 1 583545 41672 28 611700 1.3 1 576014 35686 29 633124 1.3 1 585646 47478 30 682940 1.3 1 645043 37897 31 478686 1.3 1 453370 25316 32 547238 1.3 1 517091 30147 33 480728 1.3 1 453002 27726 34 494635 1.3 1 464901 29734 35 478473 1.3 1 450875 27598 36 516849 1.3 1 482435 34414 37 434835 1.3 1 412295 22540 38 443120 1.3 1 421992 21128 39 495131 1.3 1 462666 32465 40 477920 1.3 1 452410 25510 41 459272 1.3 1 433148 26124 42 475186 1.3 1 448224 26962 43 400683 1.3 1 360302 40381 44 337660 1.3 1 314649 23011 45 582734 1.3 1 557071 25663 46 132918 1.3 1 121243 11675 47 371627 1.3 1 342725 28902 48 1091243 1.3 1 1044295 46948 49 299985 1.3 1 283170 16815 50 329730 1.3 1 307810 21920 51 625167 1.3 1 599735 25432 52 257328 1.3 1 246144 11184 53 176429 1.3 1 164483 11946 54 596995 1.3 1 575771 21224 55 353253 1.3 1 342969 10284 56 91462 1.3 1 85004 6458 57 261125 1.3 1 254649 6476 58 62075 1.3 1 57432 4643 59 205145 1.3 1 198525 6620 60 72716 1.3 1 69644 3072 61 44047 1.3 1 38550 5497 62 269383 1.3 1 259858 9525 63 178144 1.3 1 173898 4246 64 34321 1.3 1 31799 2522 65 93792 1.3 1 88420 5372 66 197851 1.3 1 191771 6080 67 81891 1.3 1 77626 4265 68 158919 1.3 1 151753 7166 69 175706 1.3 1 166085 9621 70 317748 1.3 1 306559 11189 71 44072 1.3 1 40940 3132 72 26935 1.3 1 24840 2095 73 72905 1.3 1 69530 3375 74 102266 1.3 1 97778 4488 75 106314 1.3 1 101653 4661 76 102816 1.3 1 98262 4554 77 98732 1.3 1 94231 4501 78 94526 1.3 1 90240 4286 79 91311 1.3 1 87256 4055 80 90505 1.3 1 86453 4052 81 88648 1.3 1 84856 3792 82 80885 1.3 1 77317 3568 83 71272 1.3 1 67952 3320 84 65118 1.3 1 62270 2848 85 59336 1.3 1 56764 2572 86 55373 1.3 1 52982 2391 87 51826 1.3 1 49608 2218 88 47858 1.3 1 45870 1988 89 45478 1.3 1 43602 1876 90 43556 1.3 1 41784 1772 91 41252 1.3 1 39559 1693 92 37934 1.3 1 36349 1585 93 33905 1.3 1 32454 1451 94 30752 1.3 1 29401 1351 95 27369 1.3 1 26233 1136 96 24916 1.3 1 23970 946 97 22650 1.3 1 21775 875 98 20910 1.3 1 20052 858 99 19157 1.3 1 18370 787 100 17659 1.3 1 16978 681 101 16494 1.3 1 15806 688 102 14968 1.3 1 14344 624 103 13075 1.3 1 12548 527 104 11655 1.3 1 11246 409 105 10157 1.3 1 9736 421 106 8597 1.3 1 8284 313 107 7653 1.3 1 7332 321 108 6829 1.3 1 6564 265 109 6023 1.3 1 5782 241 110 5164 1.3 1 4955 209 111 4326 1.3 1 4149 177 112 3855 1.3 1 3717 138 113 3171 1.3 1 3032 139 114 2612 1.3 1 2514 98 115 2106 1.3 1 2014 92 116 1625 1.3 1 1540 85 117 1435 1.3 1 1366 69 118 1095 1.3 1 1048 47 119 904 1.3 1 866 38 120 903 1.3 1 861 42 121 541 1.3 1 509 32 122 432 1.3 1 404 28 123 316 1.3 1 290 26 124 252 1.3 1 231 21 125 188 1.3 1 171 17 126 148 1.3 1 127 21 127 100 1.3 1 88 12 128 101 1.3 1 94 7 129 72 1.3 1 64 8 130 67 1.3 1 50 17 131 74 1.3 1 61 13 132 58 1.3 1 51 7 133 62 1.3 1 46 16 134 44 1.3 1 25 19 135 60 1.3 1 46 14 136 43 1.3 1 33 10 137 38 1.3 1 31 7 138 40 1.3 1 19 21 139 23 1.3 1 17 6 140 34 1.3 1 18 16 141 43 1.3 1 27 16 142 26 1.3 1 10 16 143 28 1.3 1 15 13 144 24 1.3 1 9 15 145 46 1.3 1 25 21 146 36 1.3 1 14 22 147 39 1.3 1 16 23 148 38 1.3 1 12 26 149 47 1.3 1 22 25 150 30 1.3 1 6 24 151 8534 1.3 1 35 8499 RUN STATISTICS FOR INPUT FILE: ERR10684006_2.fastq.gz ============================================= 84111724 sequences processed in total