SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684005_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 221306). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684005_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 1589.485 s (18.719 µs/read; 3.21 M reads/minute). === Summary === Total reads processed: 84,913,702 Reads with adapters: 49,289,909 (58.0%) Reads written (passing filters): 84,913,702 (100.0%) Total basepairs processed: 12,821,969,002 bp Quality-trimmed: 23,914,932 bp (0.2%) Total written (filtered): 11,927,756,421 bp (93.0%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 49289909 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 35.5% C: 1.9% G: 18.0% T: 44.5% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 18029833 21228425.5 0 18029833 2 3459017 5307106.4 0 3459017 3 1760900 1326776.6 0 1760900 4 1055342 331694.1 0 1055342 5 664862 82923.5 0 664862 6 591581 20730.9 0 591581 7 609410 5182.7 0 609410 8 648266 1295.7 0 648266 9 614373 323.9 0 611874 2499 10 601089 81.0 1 567335 33754 11 607390 20.2 1 562922 44468 12 597883 5.1 1 559977 37906 13 537138 1.3 1 504016 33122 14 532737 1.3 1 495791 36946 15 548555 1.3 1 514402 34153 16 527471 1.3 1 488412 39059 17 584591 1.3 1 539787 44804 18 644239 1.3 1 607167 37072 19 420034 1.3 1 396683 23351 20 518002 1.3 1 486419 31583 21 482679 1.3 1 451908 30771 22 443349 1.3 1 413967 29382 23 454834 1.3 1 425831 29003 24 427581 1.3 1 390815 36766 25 428783 1.3 1 401673 27110 26 384967 1.3 1 365611 19356 27 443867 1.3 1 416189 27678 28 445997 1.3 1 420583 25414 29 458204 1.3 1 425448 32756 30 488651 1.3 1 464114 24537 31 336761 1.3 1 320466 16295 32 367337 1.3 1 348631 18706 33 343296 1.3 1 323559 19737 34 347898 1.3 1 326947 20951 35 293542 1.3 1 281460 12082 36 326923 1.3 1 308861 18062 37 353270 1.3 1 324737 28533 38 294685 1.3 1 280935 13750 39 341375 1.3 1 322782 18593 40 335452 1.3 1 316005 19447 41 345194 1.3 1 327992 17202 42 251053 1.3 1 240742 10311 43 266400 1.3 1 247980 18420 44 234841 1.3 1 220257 14584 45 378426 1.3 1 363615 14811 46 83206 1.3 1 77365 5841 47 226014 1.3 1 215323 10691 48 288562 1.3 1 273349 15213 49 219935 1.3 1 210375 9560 50 236277 1.3 1 224280 11997 51 249906 1.3 1 235426 14480 52 212006 1.3 1 202341 9665 53 188332 1.3 1 180276 8056 54 204456 1.3 1 192737 11719 55 249070 1.3 1 237900 11170 56 122193 1.3 1 116010 6183 57 221272 1.3 1 213034 8238 58 195750 1.3 1 188684 7066 59 157934 1.3 1 150712 7222 60 188569 1.3 1 183051 5518 61 107963 1.3 1 103191 4772 62 186703 1.3 1 178303 8400 63 190721 1.3 1 184772 5949 64 104305 1.3 1 100675 3630 65 89955 1.3 1 85683 4272 66 152772 1.3 1 146881 5891 67 127258 1.3 1 122406 4852 68 164678 1.3 1 158512 6166 69 144709 1.3 1 138012 6697 70 230738 1.3 1 221932 8806 71 23097 1.3 1 21522 1575 72 40436 1.3 1 38493 1943 73 83087 1.3 1 79797 3290 74 91312 1.3 1 87629 3683 75 89118 1.3 1 85434 3684 76 85925 1.3 1 82301 3624 77 82402 1.3 1 78926 3476 78 81043 1.3 1 77584 3459 79 80208 1.3 1 76846 3362 80 80793 1.3 1 77322 3471 81 80489 1.3 1 77218 3271 82 74097 1.3 1 70985 3112 83 67878 1.3 1 65024 2854 84 62330 1.3 1 59771 2559 85 57887 1.3 1 55512 2375 86 54373 1.3 1 52231 2142 87 51503 1.3 1 49427 2076 88 49687 1.3 1 47764 1923 89 47695 1.3 1 45918 1777 90 46367 1.3 1 44684 1683 91 45102 1.3 1 43338 1764 92 42374 1.3 1 40799 1575 93 39234 1.3 1 37693 1541 94 36176 1.3 1 34759 1417 95 32865 1.3 1 31617 1248 96 29925 1.3 1 28773 1152 97 28215 1.3 1 27183 1032 98 26044 1.3 1 25090 954 99 24516 1.3 1 23597 919 100 23602 1.3 1 22707 895 101 22447 1.3 1 21584 863 102 20756 1.3 1 20003 753 103 19031 1.3 1 18390 641 104 17331 1.3 1 16695 636 105 15331 1.3 1 14777 554 106 13821 1.3 1 13308 513 107 12401 1.3 1 11935 466 108 11248 1.3 1 10793 455 109 9975 1.3 1 9590 385 110 9080 1.3 1 8761 319 111 8303 1.3 1 8007 296 112 7164 1.3 1 6874 290 113 6469 1.3 1 6237 232 114 5273 1.3 1 5084 189 115 4484 1.3 1 4308 176 116 3617 1.3 1 3479 138 117 3260 1.3 1 3156 104 118 2642 1.3 1 2544 98 119 2188 1.3 1 2074 114 120 2712 1.3 1 2632 80 121 1549 1.3 1 1479 70 122 1121 1.3 1 1082 39 123 817 1.3 1 763 54 124 638 1.3 1 594 44 125 423 1.3 1 375 48 126 418 1.3 1 390 28 127 275 1.3 1 243 32 128 192 1.3 1 164 28 129 182 1.3 1 155 27 130 119 1.3 1 97 22 131 146 1.3 1 123 23 132 96 1.3 1 74 22 133 122 1.3 1 103 19 134 70 1.3 1 59 11 135 111 1.3 1 85 26 136 76 1.3 1 58 18 137 105 1.3 1 81 24 138 60 1.3 1 42 18 139 70 1.3 1 55 15 140 62 1.3 1 44 18 141 98 1.3 1 73 25 142 65 1.3 1 31 34 143 75 1.3 1 41 34 144 58 1.3 1 21 37 145 63 1.3 1 33 30 146 76 1.3 1 31 45 147 67 1.3 1 42 25 148 57 1.3 1 25 32 149 88 1.3 1 47 41 150 104 1.3 1 12 92 151 55831 1.3 1 192 55639 RUN STATISTICS FOR INPUT FILE: ERR10684005_2.fastq.gz ============================================= 84913702 sequences processed in total