Bismark report for: ERR10684004_val_1.fq.gz and ERR10684004_val_2.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/whale/reference/GCF_963853765.1/bismark_genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 117247237 Number of paired-end alignments with a unique best hit: 70038264 Mapping efficiency: 59.7% Sequence pairs with no alignments under any condition: 28293463 Sequence pairs did not map uniquely: 18915510 Sequence pairs which were discarded because genomic sequence could not be extracted: 19 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 35013300 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 35024945 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 3465811632 Total methylated C's in CpG context: 69793339 Total methylated C's in CHG context: 20254704 Total methylated C's in CHH context: 80407534 Total methylated C's in Unknown context: 383373 Total unmethylated C's in CpG context: 392557372 Total unmethylated C's in CHG context: 603300330 Total unmethylated C's in CHH context: 2299498353 Total unmethylated C's in Unknown context: 8999910 C methylated in CpG context: 15.1% C methylated in CHG context: 3.2% C methylated in CHH context: 3.4% C methylated in Unknown context (CN or CHN): 4.1% Bismark completed in 0d 7h 48m 45s