SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684004_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 282233). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684004_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1934.809 s (16.498 µs/read; 3.64 M reads/minute). === Summary === Total reads processed: 117,273,904 Reads with adapters: 73,421,341 (62.6%) Reads written (passing filters): 117,273,904 (100.0%) Total basepairs processed: 17,708,359,504 bp Quality-trimmed: 30,454,324 bp (0.2%) Total written (filtered): 16,202,356,359 bp (91.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 73421341 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.4% C: 0.9% G: 17.4% T: 45.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 23058033 29318476.0 0 23058033 2 4274241 7329619.0 0 4274241 3 2293596 1832404.8 0 2293596 4 1470593 458101.2 0 1470593 5 975152 114525.3 0 975152 6 879745 28631.3 0 879745 7 906798 7157.8 0 906798 8 971617 1789.5 0 971617 9 939212 447.4 0 937562 1650 10 922091 111.8 1 875062 47029 11 940071 28.0 1 876849 63222 12 943568 7.0 1 889503 54065 13 838989 1.7 1 795661 43328 14 860043 1.7 1 806640 53403 15 880015 1.7 1 832821 47194 16 863090 1.7 1 805769 57321 17 953884 1.7 1 888550 65334 18 1044418 1.7 1 991363 53055 19 699461 1.7 1 665681 33780 20 856396 1.7 1 812173 44223 21 820576 1.7 1 773875 46701 22 759527 1.7 1 715919 43608 23 781458 1.7 1 736951 44507 24 755258 1.7 1 696894 58364 25 742926 1.7 1 702155 40771 26 689988 1.7 1 659905 30083 27 784669 1.7 1 742461 42208 28 779799 1.7 1 743276 36523 29 801873 1.7 1 751967 49906 30 872114 1.7 1 833089 39025 31 605425 1.7 1 582203 23222 32 663056 1.7 1 635424 27632 33 628476 1.7 1 595884 32592 34 639891 1.7 1 606490 33401 35 537821 1.7 1 521046 16775 36 584910 1.7 1 555593 29317 37 631874 1.7 1 594561 37313 38 697870 1.7 1 669366 28504 39 490255 1.7 1 472813 17442 40 620357 1.7 1 595319 25038 41 607042 1.7 1 575979 31063 42 481259 1.7 1 465757 15502 43 520190 1.7 1 487904 32286 44 432544 1.7 1 411322 21222 45 701130 1.7 1 678662 22468 46 154455 1.7 1 146350 8105 47 421941 1.7 1 406329 15612 48 483156 1.7 1 462984 20172 49 430408 1.7 1 415680 14728 50 445916 1.7 1 427727 18189 51 444569 1.7 1 423415 21154 52 398438 1.7 1 383588 14850 53 357725 1.7 1 344796 12929 54 353516 1.7 1 337134 16382 55 409707 1.7 1 391973 17734 56 261247 1.7 1 251461 9786 57 354838 1.7 1 342269 12569 58 418396 1.7 1 405046 13350 59 258359 1.7 1 247541 10818 60 376834 1.7 1 367252 9582 61 256459 1.7 1 248406 8053 62 318880 1.7 1 306807 12073 63 309165 1.7 1 300188 8977 64 250247 1.7 1 243051 7196 65 169571 1.7 1 163377 6194 66 253114 1.7 1 244840 8274 67 256267 1.7 1 248416 7851 68 317119 1.7 1 306922 10197 69 252177 1.7 1 242679 9498 70 403527 1.7 1 389056 14471 71 48855 1.7 1 46622 2233 72 77551 1.7 1 74892 2659 73 149486 1.7 1 144826 4660 74 158956 1.7 1 153978 4978 75 153577 1.7 1 148458 5119 76 145124 1.7 1 140149 4975 77 138795 1.7 1 133996 4799 78 135114 1.7 1 130588 4526 79 134949 1.7 1 130421 4528 80 134716 1.7 1 130084 4632 81 134195 1.7 1 129699 4496 82 122369 1.7 1 118186 4183 83 108121 1.7 1 104340 3781 84 98583 1.7 1 95227 3356 85 89446 1.7 1 86410 3036 86 82825 1.7 1 80101 2724 87 77134 1.7 1 74717 2417 88 72751 1.7 1 70445 2306 89 70254 1.7 1 68151 2103 90 68559 1.7 1 66481 2078 91 66358 1.7 1 64189 2169 92 60974 1.7 1 59043 1931 93 54557 1.7 1 52905 1652 94 48207 1.7 1 46706 1501 95 42967 1.7 1 41670 1297 96 38616 1.7 1 37400 1216 97 35405 1.7 1 34303 1102 98 32704 1.7 1 31736 968 99 30463 1.7 1 29542 921 100 28693 1.7 1 27834 859 101 26994 1.7 1 26216 778 102 24575 1.7 1 23841 734 103 21672 1.7 1 21011 661 104 19214 1.7 1 18689 525 105 16459 1.7 1 16006 453 106 14485 1.7 1 14037 448 107 12683 1.7 1 12295 388 108 10888 1.7 1 10557 331 109 9932 1.7 1 9602 330 110 8429 1.7 1 8202 227 111 7813 1.7 1 7532 281 112 6602 1.7 1 6398 204 113 5832 1.7 1 5631 201 114 4514 1.7 1 4390 124 115 3654 1.7 1 3538 116 116 3100 1.7 1 2988 112 117 2394 1.7 1 2306 88 118 1865 1.7 1 1787 78 119 1551 1.7 1 1495 56 120 1652 1.7 1 1599 53 121 1039 1.7 1 992 47 122 752 1.7 1 714 38 123 526 1.7 1 497 29 124 406 1.7 1 386 20 125 319 1.7 1 295 24 126 221 1.7 1 190 31 127 162 1.7 1 147 15 128 103 1.7 1 87 16 129 106 1.7 1 89 17 130 87 1.7 1 63 24 131 77 1.7 1 61 16 132 50 1.7 1 32 18 133 72 1.7 1 50 22 134 69 1.7 1 43 26 135 46 1.7 1 30 16 136 43 1.7 1 27 16 137 53 1.7 1 33 20 138 36 1.7 1 14 22 139 29 1.7 1 16 13 140 37 1.7 1 11 26 141 45 1.7 1 18 27 142 21 1.7 1 8 13 143 30 1.7 1 11 19 144 33 1.7 1 9 24 145 42 1.7 1 19 23 146 22 1.7 1 4 18 147 43 1.7 1 15 28 148 36 1.7 1 7 29 149 47 1.7 1 23 24 150 72 1.7 1 12 60 151 14773 1.7 1 58 14715 RUN STATISTICS FOR INPUT FILE: ERR10684004_1.fastq.gz ============================================= 117273904 sequences processed in total