SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684003_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 264554). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684003_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1673.061 s (15.360 µs/read; 3.91 M reads/minute). === Summary === Total reads processed: 108,922,671 Reads with adapters: 65,825,701 (60.4%) Reads written (passing filters): 108,922,671 (100.0%) Total basepairs processed: 16,447,323,321 bp Quality-trimmed: 88,444,608 bp (0.5%) Total written (filtered): 14,995,881,733 bp (91.2%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 65825701 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.0% C: 1.3% G: 17.4% T: 45.3% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 1 22061435 27230667.8 0 22061435 2 4056305 6807666.9 0 4056305 3 2052014 1701916.7 0 2052014 4 1243441 425479.2 0 1243441 5 760655 106369.8 0 760655 6 676009 26592.4 0 676009 7 689740 6648.1 0 689740 8 727870 1662.0 0 727870 9 703993 415.5 0 700075 3918 10 698411 103.9 1 659390 39021 11 715795 26.0 1 664103 51692 12 736123 6.5 1 689993 46130 13 638642 1.6 1 601693 36949 14 682986 1.6 1 633806 49180 15 664042 1.6 1 624863 39179 16 668026 1.6 1 616893 51133 17 714404 1.6 1 659946 54458 18 738935 1.6 1 697313 41622 19 543817 1.6 1 512134 31683 20 639284 1.6 1 602580 36704 21 628403 1.6 1 587881 40522 22 593178 1.6 1 555944 37234 23 622945 1.6 1 583269 39676 24 601746 1.6 1 550938 50808 25 586914 1.6 1 551444 35470 26 536454 1.6 1 508765 27689 27 594574 1.6 1 558453 36121 28 584595 1.6 1 552451 32144 29 581370 1.6 1 539955 41415 30 641294 1.6 1 609035 32259 31 462148 1.6 1 440068 22080 32 541454 1.6 1 513914 27540 33 508670 1.6 1 479945 28725 34 536720 1.6 1 504163 32557 35 530124 1.6 1 506707 23417 36 435139 1.6 1 416420 18719 37 489644 1.6 1 464021 25623 38 460775 1.6 1 431689 29086 39 486234 1.6 1 459790 26444 40 501694 1.6 1 470598 31096 41 456661 1.6 1 437144 19517 42 508413 1.6 1 480665 27748 43 510951 1.6 1 459058 51893 44 360481 1.6 1 335958 24523 45 651241 1.6 1 621817 29424 46 203337 1.6 1 186544 16793 47 462549 1.6 1 418132 44417 48 2445982 1.6 1 2347842 98140 49 2756141 1.6 1 2681788 74353 50 182319 1.6 1 171425 10894 51 276128 1.6 1 264018 12110 52 321943 1.6 1 311314 10629 53 60620 1.6 1 55112 5508 54 250875 1.6 1 244084 6791 55 70186 1.6 1 66521 3665 56 88397 1.6 1 84930 3467 57 42149 1.6 1 40097 2052 58 37426 1.6 1 35349 2077 59 35050 1.6 1 32931 2119 60 22639 1.6 1 20749 1890 61 38120 1.6 1 34351 3769 62 193559 1.6 1 188297 5262 63 29424 1.6 1 27341 2083 64 58558 1.6 1 55998 2560 65 45463 1.6 1 41919 3544 66 183823 1.6 1 178899 4924 67 29738 1.6 1 27337 2401 68 87369 1.6 1 83281 4088 69 132567 1.6 1 126166 6401 70 226194 1.6 1 218363 7831 71 103904 1.6 1 99484 4420 72 32890 1.6 1 30491 2399 73 48541 1.6 1 45752 2789 74 105681 1.6 1 101272 4409 75 153089 1.6 1 147177 5912 76 172030 1.6 1 165425 6605 77 174903 1.6 1 168023 6880 78 170263 1.6 1 163490 6773 79 167443 1.6 1 160775 6668 80 164109 1.6 1 157543 6566 81 161937 1.6 1 155429 6508 82 154550 1.6 1 148431 6119 83 143610 1.6 1 137500 6110 84 136736 1.6 1 131323 5413 85 128677 1.6 1 123695 4982 86 121355 1.6 1 116586 4769 87 114720 1.6 1 110351 4369 88 108880 1.6 1 104880 4000 89 103596 1.6 1 99783 3813 90 99532 1.6 1 95849 3683 91 95539 1.6 1 92032 3507 92 90400 1.6 1 87058 3342 93 83432 1.6 1 80227 3205 94 77017 1.6 1 74136 2881 95 70459 1.6 1 67908 2551 96 64749 1.6 1 62409 2340 97 59075 1.6 1 56904 2171 98 54353 1.6 1 52359 1994 99 50902 1.6 1 49146 1756 100 47748 1.6 1 46048 1700 101 44384 1.6 1 42811 1573 102 41734 1.6 1 40226 1508 103 37230 1.6 1 35951 1279 104 32608 1.6 1 31547 1061 105 28831 1.6 1 27820 1011 106 25407 1.6 1 24549 858 107 22641 1.6 1 21835 806 108 20400 1.6 1 19675 725 109 17949 1.6 1 17328 621 110 15363 1.6 1 14825 538 111 14116 1.6 1 13614 502 112 12379 1.6 1 11983 396 113 10509 1.6 1 10105 404 114 8832 1.6 1 8537 295 115 7309 1.6 1 7069 240 116 5871 1.6 1 5679 192 117 4958 1.6 1 4784 174 118 4008 1.6 1 3857 151 119 3211 1.6 1 3059 152 120 6085 1.6 1 5897 188 121 2349 1.6 1 2244 105 122 1554 1.6 1 1486 68 123 1092 1.6 1 1039 53 124 769 1.6 1 741 28 125 570 1.6 1 523 47 126 434 1.6 1 394 40 127 250 1.6 1 223 27 128 227 1.6 1 190 37 129 158 1.6 1 141 17 130 110 1.6 1 92 18 131 106 1.6 1 89 17 132 88 1.6 1 77 11 133 108 1.6 1 91 17 134 59 1.6 1 44 15 135 79 1.6 1 53 26 136 67 1.6 1 42 25 137 65 1.6 1 51 14 138 55 1.6 1 33 22 139 72 1.6 1 43 29 140 49 1.6 1 30 19 141 48 1.6 1 32 16 142 58 1.6 1 35 23 143 61 1.6 1 22 39 144 48 1.6 1 20 28 145 96 1.6 1 58 38 146 41 1.6 1 16 25 147 65 1.6 1 39 26 148 59 1.6 1 25 34 149 85 1.6 1 46 39 150 155 1.6 1 11 144 151 88474 1.6 1 288 88186 RUN STATISTICS FOR INPUT FILE: ERR10684003_1.fastq.gz ============================================= 108922671 sequences processed in total