SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684002_1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 260252). Second best hit was smallRNA (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684002_1.fastq.gz Processing single-end reads on 2 cores ... Finished in 1726.865 s (16.686 µs/read; 3.60 M reads/minute). === Summary === Total reads processed: 103,490,578 Reads with adapters: 64,053,242 (61.9%) Reads written (passing filters): 103,490,578 (100.0%) Total basepairs processed: 15,627,077,278 bp Quality-trimmed: 24,139,607 bp (0.2%) Total written (filtered): 14,432,821,765 bp (92.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 64053242 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 36.9% C: 0.7% G: 17.5% T: 44.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 20986930 25872644.5 0 20986930 2 3925806 6468161.1 0 3925806 3 2156411 1617040.3 0 2156411 4 1428495 404260.1 0 1428495 5 972605 101065.0 0 972605 6 852080 25266.3 0 852080 7 886113 6316.6 0 886113 8 944479 1579.1 0 944479 9 902995 394.8 0 901464 1531 10 874860 98.7 1 829750 45110 11 879437 24.7 1 818971 60466 12 883538 6.2 1 831967 51571 13 749707 1.5 1 711804 37903 14 794445 1.5 1 742313 52132 15 782700 1.5 1 741960 40740 16 796927 1.5 1 741689 55238 17 878143 1.5 1 817315 60828 18 926947 1.5 1 879667 47280 19 663784 1.5 1 630175 33609 20 754549 1.5 1 715668 38881 21 728209 1.5 1 683558 44651 22 649061 1.5 1 612348 36713 23 658898 1.5 1 620157 38741 24 649208 1.5 1 597233 51975 25 621582 1.5 1 587670 33912 26 581919 1.5 1 556269 25650 27 671721 1.5 1 634648 37073 28 656999 1.5 1 627316 29683 29 703048 1.5 1 658053 44995 30 740108 1.5 1 707935 32173 31 507037 1.5 1 487388 19649 32 558094 1.5 1 533773 24321 33 491572 1.5 1 468147 23425 34 487491 1.5 1 462464 25027 35 469641 1.5 1 450694 18947 36 442433 1.5 1 425003 17430 37 462555 1.5 1 441221 21334 38 497046 1.5 1 473646 23400 39 505253 1.5 1 482796 22457 40 623807 1.5 1 597973 25834 41 330219 1.5 1 309726 20493 42 396100 1.5 1 382888 13212 43 387446 1.5 1 362539 24907 44 342589 1.5 1 324794 17795 45 519135 1.5 1 502232 16903 46 137403 1.5 1 130616 6787 47 322930 1.5 1 311306 11624 48 363324 1.5 1 348243 15081 49 343242 1.5 1 331144 12098 50 346701 1.5 1 333016 13685 51 340970 1.5 1 324679 16291 52 303755 1.5 1 292333 11422 53 271645 1.5 1 261518 10127 54 261830 1.5 1 249582 12248 55 292445 1.5 1 279280 13165 56 203110 1.5 1 195386 7724 57 259567 1.5 1 250079 9488 58 318523 1.5 1 308022 10501 59 196671 1.5 1 188259 8412 60 290590 1.5 1 283240 7350 61 207119 1.5 1 200711 6408 62 236013 1.5 1 226958 9055 63 219611 1.5 1 212937 6674 64 189505 1.5 1 184189 5316 65 126776 1.5 1 122138 4638 66 181415 1.5 1 175489 5926 67 188713 1.5 1 182784 5929 68 232474 1.5 1 224953 7521 69 184774 1.5 1 177815 6959 70 303104 1.5 1 292279 10825 71 39272 1.5 1 37636 1636 72 58112 1.5 1 56219 1893 73 109137 1.5 1 105855 3282 74 114917 1.5 1 111270 3647 75 109968 1.5 1 106415 3553 76 104157 1.5 1 100671 3486 77 99583 1.5 1 96191 3392 78 96784 1.5 1 93594 3190 79 95044 1.5 1 91915 3129 80 95841 1.5 1 92665 3176 81 95171 1.5 1 92031 3140 82 86670 1.5 1 83795 2875 83 76744 1.5 1 74053 2691 84 68382 1.5 1 66106 2276 85 63468 1.5 1 61309 2159 86 58165 1.5 1 56336 1829 87 54163 1.5 1 52432 1731 88 51339 1.5 1 49768 1571 89 48570 1.5 1 47055 1515 90 47516 1.5 1 46054 1462 91 45412 1.5 1 44052 1360 92 41833 1.5 1 40563 1270 93 37553 1.5 1 36410 1143 94 32803 1.5 1 31822 981 95 29370 1.5 1 28532 838 96 26523 1.5 1 25707 816 97 23860 1.5 1 23151 709 98 21811 1.5 1 21155 656 99 20223 1.5 1 19653 570 100 19039 1.5 1 18490 549 101 17672 1.5 1 17182 490 102 16029 1.5 1 15568 461 103 14147 1.5 1 13739 408 104 12208 1.5 1 11851 357 105 10565 1.5 1 10258 307 106 9213 1.5 1 8930 283 107 8211 1.5 1 7972 239 108 6993 1.5 1 6786 207 109 6049 1.5 1 5852 197 110 5304 1.5 1 5154 150 111 4717 1.5 1 4573 144 112 3942 1.5 1 3803 139 113 3346 1.5 1 3246 100 114 2481 1.5 1 2417 64 115 2032 1.5 1 1967 65 116 1698 1.5 1 1645 53 117 1299 1.5 1 1247 52 118 1065 1.5 1 1020 45 119 845 1.5 1 794 51 120 855 1.5 1 814 41 121 533 1.5 1 513 20 122 364 1.5 1 351 13 123 296 1.5 1 274 22 124 197 1.5 1 173 24 125 152 1.5 1 138 14 126 117 1.5 1 103 14 127 90 1.5 1 78 12 128 90 1.5 1 80 10 129 86 1.5 1 72 14 130 74 1.5 1 64 10 131 74 1.5 1 58 16 132 54 1.5 1 48 6 133 61 1.5 1 44 17 134 47 1.5 1 36 11 135 46 1.5 1 32 14 136 38 1.5 1 26 12 137 49 1.5 1 31 18 138 39 1.5 1 26 13 139 30 1.5 1 20 10 140 31 1.5 1 16 15 141 33 1.5 1 14 19 142 19 1.5 1 6 13 143 26 1.5 1 13 13 144 20 1.5 1 7 13 145 34 1.5 1 12 22 146 26 1.5 1 9 17 147 20 1.5 1 6 14 148 33 1.5 1 7 26 149 45 1.5 1 18 27 150 43 1.5 1 13 30 151 6042 1.5 1 29 6013 RUN STATISTICS FOR INPUT FILE: ERR10684002_1.fastq.gz ============================================= 103490578 sequences processed in total