SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10684001_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 149537). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10684001_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 1958.542 s (20.965 µs/read; 2.86 M reads/minute). === Summary === Total reads processed: 93,417,878 Reads with adapters: 47,073,784 (50.4%) Reads written (passing filters): 93,417,878 (100.0%) Total basepairs processed: 14,106,099,578 bp Quality-trimmed: 27,731,137 bp (0.2%) Total written (filtered): 13,355,735,587 bp (94.7%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 47073784 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 35.0% C: 1.9% G: 16.6% T: 46.2% none/other: 0.2% Overview of removed sequences length count expect max.err error counts 1 22686129 23354469.5 0 22686129 2 4160585 5838617.4 0 4160585 3 1755561 1459654.3 0 1755561 4 856655 364913.6 0 856655 5 362857 91228.4 0 362857 6 321613 22807.1 0 321613 7 327705 5701.8 0 327705 8 345621 1425.4 0 345621 9 319894 356.4 0 318560 1334 10 330725 89.1 1 310729 19996 11 332784 22.3 1 309324 23460 12 345301 5.6 1 323462 21839 13 308644 1.4 1 290069 18575 14 322241 1.4 1 299940 22301 15 323703 1.4 1 304263 19440 16 312454 1.4 1 289767 22687 17 335309 1.4 1 310334 24975 18 343029 1.4 1 322499 20530 19 256453 1.4 1 242077 14376 20 300716 1.4 1 283393 17323 21 295532 1.4 1 276741 18791 22 286594 1.4 1 267872 18722 23 296301 1.4 1 277344 18957 24 290791 1.4 1 267022 23769 25 284679 1.4 1 267149 17530 26 258597 1.4 1 245897 12700 27 284392 1.4 1 267202 17190 28 270044 1.4 1 255118 14926 29 272538 1.4 1 253281 19257 30 294974 1.4 1 279632 15342 31 217831 1.4 1 208004 9827 32 254815 1.4 1 242557 12258 33 248776 1.4 1 234491 14285 34 264457 1.4 1 247571 16886 35 279089 1.4 1 266337 12752 36 186775 1.4 1 179380 7395 37 242066 1.4 1 229771 12295 38 242790 1.4 1 229867 12923 39 228216 1.4 1 217037 11179 40 228407 1.4 1 217288 11119 41 227607 1.4 1 213017 14590 42 216992 1.4 1 208269 8723 43 274657 1.4 1 255014 19643 44 168359 1.4 1 159466 8893 45 329260 1.4 1 318306 10954 46 59722 1.4 1 55683 4039 47 181254 1.4 1 174181 7073 48 217287 1.4 1 206864 10423 49 176098 1.4 1 169120 6978 50 180396 1.4 1 172166 8230 51 188754 1.4 1 178605 10149 52 179938 1.4 1 172135 7803 53 169372 1.4 1 162650 6722 54 174541 1.4 1 165689 8852 55 217391 1.4 1 207310 10081 56 123909 1.4 1 118531 5378 57 180585 1.4 1 173854 6731 58 185109 1.4 1 178625 6484 59 118572 1.4 1 113271 5301 60 150187 1.4 1 145967 4220 61 107309 1.4 1 103352 3957 62 158593 1.4 1 151888 6705 63 166020 1.4 1 160996 5024 64 124396 1.4 1 120345 4051 65 89704 1.4 1 85977 3727 66 146724 1.4 1 141422 5302 67 133961 1.4 1 129472 4489 68 158793 1.4 1 153355 5438 69 124096 1.4 1 119071 5025 70 185488 1.4 1 178432 7056 71 24013 1.4 1 22698 1315 72 39934 1.4 1 38377 1557 73 81204 1.4 1 78476 2728 74 91694 1.4 1 88497 3197 75 92598 1.4 1 89333 3265 76 89705 1.4 1 86393 3312 77 88005 1.4 1 84816 3189 78 84250 1.4 1 81129 3121 79 81776 1.4 1 78670 3106 80 78446 1.4 1 75567 2879 81 75479 1.4 1 72781 2698 82 72557 1.4 1 69860 2697 83 69523 1.4 1 66905 2618 84 66530 1.4 1 64198 2332 85 63655 1.4 1 61392 2263 86 60772 1.4 1 58695 2077 87 58072 1.4 1 56031 2041 88 55167 1.4 1 53281 1886 89 52625 1.4 1 50904 1721 90 50192 1.4 1 48629 1563 91 47476 1.4 1 45894 1582 92 45689 1.4 1 44180 1509 93 42576 1.4 1 41119 1457 94 40459 1.4 1 39119 1340 95 38174 1.4 1 36946 1228 96 36081 1.4 1 34902 1179 97 33735 1.4 1 32644 1091 98 31500 1.4 1 30470 1030 99 29888 1.4 1 28925 963 100 27862 1.4 1 26903 959 101 25899 1.4 1 25085 814 102 23823 1.4 1 23069 754 103 22103 1.4 1 21429 674 104 20366 1.4 1 19757 609 105 18819 1.4 1 18202 617 106 17365 1.4 1 16812 553 107 15431 1.4 1 14939 492 108 14069 1.4 1 13625 444 109 12708 1.4 1 12276 432 110 11283 1.4 1 10954 329 111 10321 1.4 1 10020 301 112 9044 1.4 1 8731 313 113 8082 1.4 1 7803 279 114 6664 1.4 1 6470 194 115 6072 1.4 1 5875 197 116 5157 1.4 1 4996 161 117 4358 1.4 1 4227 131 118 3608 1.4 1 3487 121 119 3106 1.4 1 2972 134 120 3926 1.4 1 3809 117 121 2150 1.4 1 2055 95 122 1485 1.4 1 1426 59 123 1175 1.4 1 1113 62 124 941 1.4 1 896 45 125 671 1.4 1 623 48 126 613 1.4 1 574 39 127 430 1.4 1 395 35 128 293 1.4 1 263 30 129 276 1.4 1 236 40 130 220 1.4 1 188 32 131 215 1.4 1 185 30 132 158 1.4 1 134 24 133 216 1.4 1 193 23 134 141 1.4 1 116 25 135 174 1.4 1 147 27 136 145 1.4 1 113 32 137 154 1.4 1 137 17 138 110 1.4 1 84 26 139 120 1.4 1 90 30 140 106 1.4 1 78 28 141 153 1.4 1 116 37 142 88 1.4 1 55 33 143 79 1.4 1 54 25 144 67 1.4 1 34 33 145 84 1.4 1 50 34 146 55 1.4 1 29 26 147 93 1.4 1 68 25 148 85 1.4 1 47 38 149 141 1.4 1 86 55 150 158 1.4 1 37 121 151 80430 1.4 1 254 80176 RUN STATISTICS FOR INPUT FILE: ERR10684001_2.fastq.gz ============================================= 93417878 sequences processed in total