SUMMARISING RUN PARAMETERS ========================== Input filename: ERR10683997_2.fastq.gz Trimming mode: paired-end Trim Galore version: 0.6.10 Cutadapt version: 4.2 Python version: could not detect Number of cores used for trimming: 2 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Illumina adapter for trimming (count: 131711). Second best hit was Nextera (count: 0) Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed This is cutadapt 4.2 with Python 3.10.8 Command line parameters: -j 2 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC ERR10683997_2.fastq.gz Processing single-end reads on 2 cores ... Finished in 1886.362 s (18.705 µs/read; 3.21 M reads/minute). === Summary === Total reads processed: 100,850,601 Reads with adapters: 49,322,000 (48.9%) Reads written (passing filters): 100,850,601 (100.0%) Total basepairs processed: 15,228,440,751 bp Quality-trimmed: 25,417,525 bp (0.2%) Total written (filtered): 14,529,940,907 bp (95.4%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 49322000 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 35.4% C: 0.5% G: 16.2% T: 47.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 25243543 25212650.2 0 25243543 2 4469098 6303162.6 0 4469098 3 1878181 1575790.6 0 1878181 4 950876 393947.7 0 950876 5 354282 98486.9 0 354282 6 307850 24621.7 0 307850 7 311431 6155.4 0 311431 8 327132 1538.9 0 327132 9 316094 384.7 0 313284 2810 10 313934 96.2 1 293410 20524 11 323025 24.0 1 296440 26585 12 336181 6.0 1 312714 23467 13 291226 1.5 1 272250 18976 14 314885 1.5 1 289956 24929 15 307788 1.5 1 287766 20022 16 309124 1.5 1 282640 26484 17 329168 1.5 1 301605 27563 18 335389 1.5 1 314383 21006 19 242892 1.5 1 226781 16111 20 288482 1.5 1 269599 18883 21 291622 1.5 1 269926 21696 22 272955 1.5 1 253549 19406 23 290553 1.5 1 269666 20887 24 284104 1.5 1 257664 26440 25 277642 1.5 1 259402 18240 26 249874 1.5 1 235247 14627 27 278645 1.5 1 259652 18993 28 263399 1.5 1 247044 16355 29 269383 1.5 1 247569 21814 30 295979 1.5 1 280348 15631 31 201162 1.5 1 190055 11107 32 249760 1.5 1 235357 14403 33 240931 1.5 1 226078 14853 34 249670 1.5 1 234189 15481 35 241687 1.5 1 227867 13820 36 233655 1.5 1 220288 13367 37 235667 1.5 1 217812 17855 38 231557 1.5 1 218379 13178 39 228918 1.5 1 215206 13712 40 275270 1.5 1 264117 11153 41 150890 1.5 1 137113 13777 42 218375 1.5 1 206303 12072 43 253215 1.5 1 234334 18881 44 167208 1.5 1 156077 11131 45 318565 1.5 1 306681 11884 46 69642 1.5 1 63796 5846 47 186705 1.5 1 177060 9645 48 206605 1.5 1 195596 11009 49 173518 1.5 1 165234 8284 50 179454 1.5 1 169483 9971 51 182269 1.5 1 170893 11376 52 173785 1.5 1 165225 8560 53 166634 1.5 1 158324 8310 54 171091 1.5 1 160384 10707 55 201220 1.5 1 190807 10413 56 124811 1.5 1 117975 6836 57 171775 1.5 1 163413 8362 58 189385 1.5 1 181775 7610 59 106117 1.5 1 99847 6270 60 151991 1.5 1 146866 5125 61 105599 1.5 1 100399 5200 62 147025 1.5 1 139461 7564 63 154172 1.5 1 148201 5971 64 126301 1.5 1 121579 4722 65 87979 1.5 1 83040 4939 66 133353 1.5 1 127466 5887 67 132356 1.5 1 126433 5923 68 153858 1.5 1 147331 6527 69 119519 1.5 1 112666 6853 70 176291 1.5 1 169508 6783 71 23423 1.5 1 21825 1598 72 37356 1.5 1 35171 2185 73 75558 1.5 1 72125 3433 74 85025 1.5 1 81389 3636 75 85479 1.5 1 81707 3772 76 82825 1.5 1 79082 3743 77 80764 1.5 1 77047 3717 78 77232 1.5 1 73774 3458 79 74162 1.5 1 70807 3355 80 71450 1.5 1 68206 3244 81 68295 1.5 1 65294 3001 82 65088 1.5 1 62092 2996 83 61900 1.5 1 58999 2901 84 59037 1.5 1 56451 2586 85 55941 1.5 1 53478 2463 86 52666 1.5 1 50385 2281 87 49998 1.5 1 47734 2264 88 47867 1.5 1 45823 2044 89 44726 1.5 1 42846 1880 90 42533 1.5 1 40767 1766 91 39725 1.5 1 38111 1614 92 37282 1.5 1 35711 1571 93 34802 1.5 1 33395 1407 94 32513 1.5 1 31209 1304 95 30328 1.5 1 29051 1277 96 28437 1.5 1 27316 1121 97 26395 1.5 1 25337 1058 98 24371 1.5 1 23431 940 99 22502 1.5 1 21623 879 100 21061 1.5 1 20178 883 101 19042 1.5 1 18300 742 102 17118 1.5 1 16446 672 103 15536 1.5 1 14900 636 104 14202 1.5 1 13602 600 105 12655 1.5 1 12145 510 106 11352 1.5 1 10908 444 107 9901 1.5 1 9535 366 108 8672 1.5 1 8330 342 109 7950 1.5 1 7631 319 110 6642 1.5 1 6356 286 111 5842 1.5 1 5584 258 112 5149 1.5 1 4945 204 113 4189 1.5 1 4017 172 114 3588 1.5 1 3429 159 115 2975 1.5 1 2842 133 116 2552 1.5 1 2439 113 117 1960 1.5 1 1865 95 118 1599 1.5 1 1516 83 119 1282 1.5 1 1215 67 120 1459 1.5 1 1383 76 121 870 1.5 1 826 44 122 614 1.5 1 578 36 123 458 1.5 1 430 28 124 449 1.5 1 415 34 125 295 1.5 1 259 36 126 296 1.5 1 265 31 127 186 1.5 1 161 25 128 161 1.5 1 140 21 129 195 1.5 1 172 23 130 126 1.5 1 114 12 131 137 1.5 1 125 12 132 105 1.5 1 93 12 133 121 1.5 1 105 16 134 80 1.5 1 69 11 135 99 1.5 1 87 12 136 77 1.5 1 60 17 137 80 1.5 1 71 9 138 53 1.5 1 38 15 139 65 1.5 1 51 14 140 49 1.5 1 31 18 141 52 1.5 1 37 15 142 38 1.5 1 27 11 143 47 1.5 1 31 16 144 41 1.5 1 24 17 145 40 1.5 1 26 14 146 30 1.5 1 16 14 147 59 1.5 1 34 25 148 40 1.5 1 19 21 149 65 1.5 1 33 32 150 59 1.5 1 19 40 151 11885 1.5 1 52 11833 RUN STATISTICS FOR INPUT FILE: ERR10683997_2.fastq.gz ============================================= 100850601 sequences processed in total