---
title: "April Daily Posts"
author: "Megan Ewing"
date: "04-04-2024"
categories: daily
format: 
  html:
    toc: true
editor: visual
---

## April 1

-   541 reading/quiz

## April 2

-   class

## April 3

-   541 reading/quiz

-   oyster methods updates

## April 4

-   reread 541 paper for more thorough notes

-   Started manila clam methods edits

-   class, lab meeting, pub a thon

-   starting to brainstorm and ask around about ways to get involved with outreach incl. open house, maritime HS, and SEAS

## April 8

-   Not Roberts lab related, but spent most of the day working on revisions for an old manuscript that are due next week

-   Class prep work

## April 9

-   Went out to Manchester for the day to help Mac with little neck clam spawning. The spawning was unsuccessful, sadly, and we'll have to re attempt in 2 weeks with hopes that 1) the clams are ripe enough and 2) there won't be excess mortality before we have a chance to spawn them

## April 10th

-   class prep / homework

## April 11th

-   got the repo together for the oyster project

-   pub a thon

-   class

-   more manila clam edits

## April 15th

-   class prep/homework

## April 16th

-   class / lab

-   start blast for o. lurida

## April 17th

-   class prep/homework

## April 18th

-   lab meeting

-   class

-   hisat work

## April 19th

-   finish hisat / submit lab

    -   hisat output interrupted by server space

## April 22nd

-   class prep/homework

## April 23rd

-   Spawning at Manchester. Went better than last time. Had both treatments go through temperature cycles of 30 minutes at 25C and 30 minutes at 15C. After planned four cycles had 2 spawners (1 male, 1 female) from both control and OA treatments. At the end of the fourth cycle, more started spawning and added 1 additional 30/30 cycle and a shorter 15/15 cycle which yielded additional males and females for both treatments. Ended with 5 total females available for crossing, est. 5 million eggs/potential larvae. I had to leave early to catch the ferry and didn't get the final counts though.

## April 24th

-   class prep/homework

## April 25th

-   pub-a-thon

-   re run deseq2 / troubleshoot why my heatmap turned out funky

-   class

-   lab meeting

-   co work with zach to get on the lurida blast / finish it