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Megans-MBP-3:~ meganewing$ /Users/meganewing/ncbi-blast-2.10.1+/bin/blastx -query /Users/meganewing/BlastTutorial/example.fasta -db /Users/meganewing/uniprot_sprot_database_DATE -out 2020-06-15-Megan-uniprot-blastx.tsv -evalue 1E-10 -outfmt 6
Megans-MBP-3:~ meganewing$ head 2020-06-05-Megan-uniprot-blastx.tsv
head: 2020-06-05-Megan-uniprot-blastx.tsv: No such file or directory
Megans-MBP-3:~ meganewing$ head /Users/meganewing/2020-06-15-Megan-uniprot-blastx.tab 
Megans-MBP-3:~ meganewing$ clear










Megans-MBP-3:~ meganewing$ /Users/meganewing/ncbi-blast-2.10.1+/bin/makeblastdb -in /Users/meganewing/BlastTutorial/example.fasta -dbtype prot -out uniprot_sprot_database2_DATE


Building a new DB, current time: 06/15/2020 16:20:48
New DB name:   /Users/meganewing/uniprot_sprot_database2_DATE
New DB title:  /Users/meganewing/BlastTutorial/example.fasta
Sequence type: Protein
Keep MBits: T
Maximum file size: 1000000000B
Adding sequences from FASTA; added 1 sequences in 0.000285864 seconds.


Megans-MBP-3:~ meganewing$ ls
2020-06-15-Megan-uniprot-blastx.tab	uniprot_sprot_database2_DATE.pdb
2020-06-15-Megan-uniprot-blastx.tsv	uniprot_sprot_database2_DATE.phr
Applications				uniprot_sprot_database2_DATE.pin
BlastTutorial				uniprot_sprot_database2_DATE.pot
Desktop					uniprot_sprot_database2_DATE.psq
Documents				uniprot_sprot_database2_DATE.ptf
Downloads				uniprot_sprot_database2_DATE.pto
Library					uniprot_sprot_database_DATE.pdb
Movies					uniprot_sprot_database_DATE.phr
Music					uniprot_sprot_database_DATE.pin
Pictures				uniprot_sprot_database_DATE.pot
Projects				uniprot_sprot_database_DATE.psq
Public					uniprot_sprot_database_DATE.ptf
SimUText 2019-2020			uniprot_sprot_database_DATE.pto
ncbi-blast-2.10.1+
Megans-MBP-3:~ meganewing$ /Users/meganewing/ncbi-blast-2.10.1+/bin/blastx -query /Users/meganewing/uniprot_sprot_database2_DATE -out 2020-03-12-Megan-uniprot-blastx.tab -evalue 1E-10 -outfmt 6
BLAST query/options error: Either a BLAST database or subject sequence(s) must be specified
Please refer to the BLAST+ user manual.
Megans-MBP-3:~ meganewing$ /Users/meganewing/ncbi-blast-2.10.1+/bin/blastx -query /Users/meganewing/BlastTutorial/example.fasta -db /Users/meganewing/uniprot_sprot_database2_DATE -out 2020-03-12-Megan-uniprot-blastx.tab -evalue 1E-10 -outfmt 6
Megans-MBP-3:~ meganewing$ head /Users/meganewing/2020-03-12-Megan-uniprot-blastx.tab 
Megans-MBP-3:~ meganewing$