FastQCFastQC Report
Tue 11 Feb 2025
PSC-0535_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePSC-0535_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35982788
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2694720.7488913866262947No Hit
CGGGAATCAAGCTGTCTCTTTCTTATACCTGGAAGTTAATATTTTTGTGA736820.2047701250942534No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)