Celeste Valdivia’s Open Lab Notebook - The Tough Tunicate

amino acid supplementation to media

Table 1 Compositions of M1 and OpM1 Media

Exposure 2 in vivo NiCl₂ x Botryllus schlosseri

nickel chloride

Divided animals into separate system, 3 days prior to start of exposure, 8 days acclimated to the recirculating system.

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Lab Reared Botryllus schlosseri Care and Maintenance

This protocol is for weekly care of lab born Botryllus schlosseri. Field sourced tunicates are brought in during the months of May-October and allowed to release oozooids on glass slides. Eventually, you want to aim to only have one…

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Pilot 1.2 in vivo NiCl₂ x Botryllus schlosseri Exposure

nickel chloride

Divided animals into separate system, 3 days prior to start of exposure, 8 days acclimated to the recirculating system.

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Use of Genetic Algorithm in Cell Culture Media Optimization

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Designing Tunicate Primers

nickel chloride

I am interested in designing primers for Botryllus schlosseri to evaluate differences in expression of apoptosis, stemness, oxidative stress, and senescence related…

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Substrate Options for the in vitro Culture of Botryllus schlosseri Epithelial Cells

Exploring of the physiology of the extra cellular matrix (ECM) of the marine invertebrate, ascidian, Botryllus schlosseri as to identify potential substrates for in vitro primary epithelial cell culturing of the same…

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Pilot 1.1 in vivo NiCl₂ x Botryllus schlosseri Exposure

nickel chloride

First pilot study for NiCl₂ exposures on whole field-sourced Botryllus schlosseri colonies.

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Trypsinization of Botryllus schlosseri Epithelial Cells

Trypsinization of cell cultures is a common method use in cell biology to disassociate adherent cells in vitro from the vessel in which they are being cultured for the purpose of passaging the cells…

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Masters Thesis Progress

This page is intended to act as a living document recording the progression of my thesis work.

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Botryllus schlosseri Epithelial Tissue Isolation Protocol

This protocol is version 08.21.23

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Cell Isolation Trial 18: Cultrex Substrates

In this small scale experiment, resorbing zooids and take over zooids, two distinct asexual developmental stages sourced from the same Botryllus individual…

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Cell Isolation Trial 17

Here I both describe the procedure that occurred for epithelial tissue isolation from Botryllus schlosseri for trial 16 and maintain this…

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Cell Isolation Trial 16

Here I both describe the procedure that occurred for epithelial tissue isolation from Botryllus schlosseri for trial 16 and maintain this…

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First Aquatic Invertebrate Cell Line

Here I summarize key takeaways and implications from a paper that describes the establishment of the only aquatic invertebrate cell line. The author is Eder L. Hansen.

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Example of Post With Code

This is an example post of the work I did for Assignment 5 for FISH 549 where I generated an exploratory and expository plot of a data set provided by the class.

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