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150463, "ATACT": 144803, "ATACC": 47572, "ATACG": 1079835, "ATAGA": 20743796, "ATAGT": 23893229, "ATAGC": 242753, "ATAGG": 9764121, "ATTAA": 42115164, "ATTAT": 58608401, "ATTAC": 538958, "ATTAG": 20034178, "ATTTA": 59270734, "ATTTT": 108724082, "ATTTC": 1367511, "ATTTG": 35153560, "ATTCA": 219739, "ATTCT": 466138, "ATTCC": 297400, "ATTCG": 1514662, "ATTGA": 24512844, "ATTGT": 34817600, "ATTGC": 267891, "ATTGG": 12139931, "ATCAA": 155431, "ATCAT": 184738, "ATCAC": 30463, "ATCAG": 71724, "ATCTA": 687585, "ATCTT": 387731, "ATCTC": 283031, "ATCTG": 609172, "ATCCA": 25960, "ATCCT": 250975, "ATCCC": 309074, "ATCCG": 11109, "ATCGA": 1114320, "ATCGT": 1362425, "ATCGC": 81072, "ATCGG": 3326380, "ATGAA": 20980187, "ATGAT": 23605202, "ATGAC": 255905, "ATGAG": 7396653, "ATGTA": 30038933, "ATGTT": 34448292, "ATGTC": 403411, "ATGTG": 13627928, "ATGCA": 63402, "ATGCT": 77182, "ATGCC": 46152, "ATGCG": 397622, "ATGGA": 9746725, "ATGGT": 12336296, "ATGGC": 161694, "ATGGG": 5016674, "ACAAA": 146819, 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5527177, "AGGCA": 23643, "AGGCT": 35081, "AGGCC": 21192, "AGGCG": 197095, "AGGGA": 4037626, "AGGGT": 5376361, "AGGGC": 82738, "AGGGG": 4241100, "TAAAA": 46340108, "TAAAT": 53056563, "TAAAC": 468229, "TAAAG": 18883761, "TAATA": 45636994, "TAATT": 55625395, "TAATC": 638193, "TAATG": 23760268, "TAACA": 118436, "TAACT": 162453, "TAACC": 56451, "TAACG": 863457, "TAAGA": 14969788, "TAAGT": 18884926, "TAAGC": 177215, "TAAGG": 8499340, "TATAA": 47806209, "TATAT": 79711431, "TATAC": 646520, "TATAG": 26974657, "TATTA": 58683072, "TATTT": 100456989, "TATTC": 1110434, "TATTG": 36202017, "TATCA": 185180, "TATCT": 1414283, "TATCC": 259022, "TATCG": 1612975, "TATGA": 22761471, "TATGT": 37332272, "TATGC": 279836, "TATGG": 12550176, "TACAA": 126665, "TACAT": 188399, "TACAC": 27288, "TACAG": 72069, "TACTA": 1195848, "TACTT": 244799, "TACTC": 78304, "TACTG": 69562, "TACCA": 33368, "TACCT": 61345, "TACCC": 74320, "TACCG": 9562, "TACGA": 1007660, "TACGT": 1402793, "TACGC": 66862, "TACGG": 626022, "TAGAA": 18851635, "TAGAT": 24134468, "TAGAC": 259272, "TAGAG": 10408422, "TAGTA": 21568005, "TAGTT": 28387108, "TAGTC": 372092, "TAGTG": 13797311, "TAGCA": 50367, "TAGCT": 71404, "TAGCC": 32763, "TAGCG": 500145, "TAGGA": 8443832, "TAGGT": 12084135, "TAGGC": 119636, "TAGGG": 6182417, "TTAAA": 53998875, "TTAAT": 55254860, "TTAAC": 526917, "TTAAG": 19346019, "TTATA": 65318475, "TTATT": 88858651, "TTATC": 1110832, "TTATG": 30424322, "TTACA": 170488, "TTACT": 242262, "TTACC": 81789, "TTACG": 1397853, "TTAGA": 22357942, "TTAGT": 27744024, "TTAGC": 282549, "TTAGG": 11540398, "TTTAA": 65248559, "TTTAT": 93800845, "TTTAC": 976636, "TTTAG": 30610792, "TTTTA": 100614982, "TTTTT": 184212640, "TTTTC": 3365455, "TTTTG": 58986512, "TTTCA": 701776, "TTTCT": 1536719, "TTTCC": 964545, "TTTCG": 2747687, "TTTGA": 38233022, "TTTGT": 54620139, "TTTGC": 491474, "TTTGG": 20198096, "TTCAA": 464930, "TTCAT": 400439, "TTCAC": 112116, "TTCAG": 228554, "TTCTA": 372793, "TTCTT": 1385429, "TTCTC": 865371, "TTCTG": 268056, "TTCCA": 213178, "TTCCT": 744457, "TTCCC": 1019971, "TTCCG": 124672, "TTCGA": 1723397, "TTCGT": 2224331, "TTCGC": 113341, "TTCGG": 1171853, "TTGAA": 28407983, "TTGAT": 35324076, "TTGAC": 319438, "TTGAG": 11589079, "TTGTA": 35904625, "TTGTT": 51871469, "TTGTC": 634663, "TTGTG": 19143962, "TTGCA": 99917, "TTGCT": 181400, "TTGCC": 56588, "TTGCG": 592773, "TTGGA": 13109216, "TTGGT": 17846815, "TTGGC": 168565, "TTGGG": 7818798, "TCAAA": 347091, "TCAAT": 255509, "TCAAC": 97075, "TCAAG": 100936, "TCATA": 208925, "TCATT": 363783, "TCATC": 68953, "TCATG": 123181, "TCACA": 32738, "TCACT": 1359247, "TCACC": 54837, "TCACG": 19429, "TCAGA": 531993, "TCAGT": 140896, "TCAGC": 15397, "TCAGG": 58065, "TCTAA": 203486, "TCTAT": 309228, "TCTAC": 41201, "TCTAG": 722135, "TCTTA": 265494, "TCTTT": 1352127, "TCTTC": 955744, "TCTTG": 199443, "TCTCA": 169806, "TCTCT": 760006, "TCTCC": 1032373, "TCTCG": 34861, "TCTGA": 1806334, "TCTGT": 204040, "TCTGC": 50152, "TCTGG": 582364, "TCCAA": 60100, "TCCAT": 41671, "TCCAC": 31894, "TCCAG": 1929421, "TCCTA": 142793, "TCCTT": 563719, "TCCTC": 921042, "TCCTG": 34716, "TCCCA": 660277, "TCCCT": 591352, "TCCCC": 1054278, "TCCCG": 16481, "TCCGA": 101607, "TCCGT": 32298, "TCCGC": 10652, "TCCGG": 12899, "TCGAA": 1058761, "TCGAT": 1548589, "TCGAC": 81485, "TCGAG": 777551, "TCGTA": 1369515, "TCGTT": 2221924, "TCGTC": 228087, "TCGTG": 778151, "TCGCA": 11115, "TCGCT": 16478, "TCGCC": 7779, "TCGCG": 210064, "TCGGA": 3320341, "TCGGT": 1005112, "TCGGC": 61755, "TCGGG": 536238, "TGAAA": 26441682, "TGAAT": 23290649, "TGAAC": 1813654, "TGAAG": 9886690, "TGATA": 25916889, "TGATT": 32275858, "TGATC": 388429, "TGATG": 14102845, "TGACA": 64413, "TGACT": 94177, "TGACC": 35896, "TGACG": 571825, "TGAGA": 9012591, "TGAGT": 11411199, "TGAGC": 115349, "TGAGG": 4604423, "TGTAA": 26256973, "TGTAT": 41208928, "TGTAC": 353177, "TGTAG": 13401588, "TGTTA": 32548719, "TGTTT": 52959214, "TGTTC": 654264, "TGTTG": 18893138, "TGTCA": 150337, "TGTCT": 282226, "TGTCC": 144674, "TGTCG": 882426, "TGTGA": 13403418, "TGTGT": 22991932, "TGTGC": 196599, "TGTGG": 7185177, "TGCAA": 72288, "TGCAT": 90499, "TGCAC": 12057, "TGCAG": 37803, "TGCTA": 67458, "TGCTT": 192064, "TGCTC": 51610, "TGCTG": 42886, "TGCCA": 16355, "TGCCT": 46294, "TGCCC": 50729, "TGCCG": 61472, "TGCGA": 421771, "TGCGT": 553195, "TGCGC": 35902, "TGCGG": 236269, "TGGAA": 10592356, "TGGAT": 12214287, "TGGAC": 121069, "TGGAG": 5267354, "TGGTA": 12318974, "TGGTT": 17064134, "TGGTC": 243324, "TGGTG": 8016681, "TGGCA": 40357, "TGGCT": 52918, "TGGCC": 22678, "TGGCG": 431156, "TGGGA": 5305469, "TGGGT": 6805981, "TGGGC": 85301, "TGGGG": 4391746, "CAAAA": 275654, "CAAAT": 205815, "CAAAC": 87862, "CAAAG": 83220, "CAATA": 141055, "CAATT": 215444, "CAATC": 39293, "CAATG": 79143, "CAACA": 33858, "CAACT": 79522, "CAACC": 55253, "CAACG": 18681, "CAAGA": 60643, "CAAGT": 85163, "CAAGC": 9164, "CAAGG": 38658, "CATAA": 133358, "CATAT": 216343, "CATAC": 25369, "CATAG": 71171, "CATTA": 155799, "CATTT": 356169, "CATTC": 54923, "CATTG": 102639, "CATCA": 36438, "CATCT": 69150, "CATCC": 37964, "CATCG": 12772, "CATGA": 70258, "CATGT": 122427, "CATGC": 8376, "CATGG": 41631, "CACAA": 33379, "CACAT": 36201, "CACAC": 2034266, "CACAG": 14586, "CACTA": 26013, "CACTT": 93831, "CACTC": 49466, "CACTG": 1255139, "CACCA": 44560, "CACCT": 29306, "CACCC": 51187, "CACCG": 10391, "CACGA": 13610, "CACGT": 1911550, "CACGC": 9218, "CACGG": 7638, "CAGAA": 77753, "CAGAT": 2219590, "CAGAC": 14848, "CAGAG": 37878, "CAGTA": 66419, "CAGTT": 124052, "CAGTC": 1365690, "CAGTG": 47736, "CAGCA": 9214, "CAGCT": 16704, "CAGCC": 9953, "CAGCG": 4392, "CAGGA": 32196, "CAGGT": 52548, "CAGGC": 5133, "CAGGG": 27890, "CTAAA": 164531, "CTAAT": 144961, "CTAAC": 35902, "CTAAG": 50705, "CTATA": 184465, "CTATT": 232303, "CTATC": 1061463, "CTATG": 85540, "CTACA": 25494, "CTACT": 39747, "CTACC": 24530, "CTACG": 9251, "CTAGA": 410550, "CTAGT": 395885, "CTAGC": 8467, "CTAGG": 232037, "CTTAA": 164239, "CTTAT": 196734, "CTTAC": 37555, "CTTAG": 116502, "CTTTA": 250647, "CTTTT": 1268133, "CTTTC": 481826, "CTTTG": 314895, "CTTCA": 158824, "CTTCT": 639015, "CTTCC": 735342, "CTTCG": 25220, "CTTGA": 105438, "CTTGT": 156992, "CTTGC": 16966, "CTTGG": 76859, "CTCAA": 82058, "CTCAT": 51969, "CTCAC": 44073, "CTCAG": 344954, "CTCTA": 118808, "CTCTT": 563045, "CTCTC": 756591, "CTCTG": 28763, "CTCCA": 1858440, "CTCCT": 566515, "CTCCC": 892007, "CTCCG": 11605, "CTCGA": 19315, "CTCGT": 25187, "CTCGC": 6880, "CTCGG": 12429, "CTGAA": 1731697, "CTGAT": 80838, "CTGAC": 19124, "CTGAG": 1229881, "CTGTA": 91163, "CTGTT": 171007, "CTGTC": 29109, "CTGTG": 55414, "CTGCA": 12603, "CTGCT": 43707, "CTGCC": 10370, "CTGCG": 5788, "CTGGA": 50793, "CTGGT": 288974, "CTGGC": 6264, "CTGGG": 286799, "CCAAA": 104261, "CCAAT": 39193, "CCAAC": 53721, "CCAAG": 22616, "CCATA": 26673, "CCATT": 49763, "CCATC": 39362, "CCATG": 11966, "CCACA": 25996, "CCACT": 48212, "CCACC": 40974, "CCACG": 13031, "CCAGA": 1790273, "CCAGT": 1384093, "CCAGC": 10961, "CCAGG": 12920, "CCTAA": 38336, "CCTAT": 36144, "CCTAC": 25370, "CCTAG": 271333, "CCTTA": 73944, "CCTTT": 399903, "CCTTC": 457229, "CCTTG": 28544, "CCTCA": 261906, "CCTCT": 527335, "CCTCC": 758169, "CCTCG": 8901, "CCTGA": 23738, "CCTGT": 24250, "CCTGC": 8317, "CCTGG": 10961, "CCCAA": 87449, "CCCAT": 40067, "CCCAC": 49437, "CCCAG": 1318580, "CCCTA": 208913, "CCCTT": 301057, "CCCTC": 542484, "CCCTG": 16283, "CCCCA": 834004, "CCCCT": 352788, "CCCCC": 621786, "CCCCG": 14649, "CCCGA": 16675, "CCCGT": 16257, "CCCGC": 6253, "CCCGG": 5163, "CCGAA": 20201, "CCGAT": 93616, "CCGAC": 7056, "CCGAG": 10917, "CCGTA": 11973, "CCGTT": 46429, "CCGTC": 65961, "CCGTG": 9361, "CCGCA": 6438, "CCGCT": 7403, "CCGCC": 6090, "CCGCG": 2926, "CCGGA": 11588, "CCGGT": 11625, "CCGGC": 5646, "CCGGG": 6108, "CGAAA": 847382, "CGAAT": 834041, "CGAAC": 51149, "CGAAG": 397565, "CGATA": 1119107, "CGATT": 1325382, "CGATC": 184941, "CGATG": 475352, "CGACA": 8263, "CGACT": 12330, "CGACC": 7428, "CGACG": 167191, "CGAGA": 535784, "CGAGT": 617687, "CGAGC": 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57303, "CTGAT": 35560, "CTGAC": 20724, "CTGAG": 21613, "CTGTA": 38875, "CTGTT": 31137, "CTGTC": 19876, "CTGTG": 12215, "CTGCA": 19422, "CTGCT": 13081, "CTGCC": 10908, "CTGCG": 2751, "CTGGA": 27292, "CTGGT": 2431, "CTGGC": 2041, "CTGGG": 60140, "CCAAA": 19487881, "CCAAT": 11428142, "CCAAC": 6572493, "CCAAG": 61998, "CCATA": 12007421, "CCATT": 8841705, "CCATC": 4757711, "CCATG": 27905, "CCACA": 7053702, "CCACT": 4450549, "CCACC": 4142427, "CCACG": 246062, "CCAGA": 146461, "CCAGT": 21115, "CCAGC": 13706, "CCAGG": 4879, "CCTAA": 10772642, "CCTAT": 9014054, "CCTAC": 4872291, "CCTAG": 66269, "CCTTA": 7791526, "CCTTT": 7419837, "CCTTC": 3632749, "CCTTG": 16216, "CCTCA": 4378510, "CCTCT": 4103676, "CCTCC": 2737153, "CCTCG": 259941, "CCTGA": 23073, "CCTGT": 18193, "CCTGC": 11210, "CCTGG": 3741, "CCCAA": 7646357, "CCCAT": 4619799, "CCCAC": 3624513, "CCCAG": 71199, "CCCTA": 5478539, "CCCTT": 3735921, "CCCTC": 2714931, "CCCTG": 16023, "CCCCA": 4266971, "CCCCT": 3243723, "CCCCC": 7329089, "CCCCG": 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7627018, "AGTTA": 17689021, "AGTTT": 28759280, "AGTTC": 371432, "AGTTG": 10354965, "AGTCA": 433291, "AGTCT": 117811, "AGTCC": 79241, "AGTCG": 470233, "AGTGA": 9223756, "AGTGT": 12554622, "AGTGC": 93226, "AGTGG": 4255429, "AGCAA": 36091, "AGCAT": 43542, "AGCAC": 392456, "AGCAG": 19838, "AGCTA": 30928, "AGCTT": 60198, "AGCTC": 31618, "AGCTG": 16351, "AGCCA": 4567, "AGCCT": 27778, "AGCCC": 32222, "AGCCG": 2212, "AGCGA": 360524, "AGCGT": 411890, "AGCGC": 25973, "AGCGG": 204840, "AGGAA": 7330942, "AGGAT": 7052781, "AGGAC": 74843, "AGGAG": 3548953, "AGGTA": 8032581, "AGGTT": 11151616, "AGGTC": 147959, "AGGTG": 5027991, "AGGCA": 17715, "AGGCT": 26566, "AGGCC": 13125, "AGGCG": 177666, "AGGGA": 3648552, "AGGGT": 4847056, "AGGGC": 62327, "AGGGG": 3071999, "TAAAA": 42783843, "TAAAT": 49002088, "TAAAC": 417604, "TAAAG": 17360276, "TAATA": 42096448, "TAATT": 51343018, "TAATC": 577592, "TAATG": 21873122, "TAACA": 100426, "TAACT": 132620, "TAACC": 42437, "TAACG": 790461, "TAAGA": 13763704, "TAAGT": 17385821, "TAAGC": 160197, "TAAGG": 7794430, "TATAA": 44122696, "TATAT": 73472143, "TATAC": 587110, "TATAG": 24787765, "TATTA": 54223097, "TATTT": 92884532, "TATTC": 1012855, "TATTG": 33338023, "TATCA": 159247, "TATCT": 729432, "TATCC": 234257, "TATCG": 1471549, "TATGA": 20980811, "TATGT": 34397257, "TATGC": 242573, "TATGG": 11542415, "TACAA": 107427, "TACAT": 162556, "TACAC": 15659, "TACAG": 61046, "TACTA": 472614, "TACTT": 201506, "TACTC": 61720, "TACTG": 58480, "TACCA": 21158, "TACCT": 47006, "TACCC": 59708, "TACCG": 6378, "TACGA": 923874, "TACGT": 1285432, "TACGC": 59232, "TACGG": 572526, "TAGAA": 17305687, "TAGAT": 21793120, "TAGAC": 234705, "TAGAG": 9498406, "TAGTA": 19796386, "TAGTT": 26082118, "TAGTC": 335824, "TAGTG": 12487992, "TAGCA": 42457, "TAGCT": 61179, "TAGCC": 28809, "TAGCG": 456945, "TAGGA": 7713568, "TAGGT": 11069443, "TAGGC": 105270, "TAGGG": 5553418, "TTAAA": 50017751, "TTAAT": 51223906, "TTAAC": 472440, "TTAAG": 17890251, "TTATA": 60634298, "TTATT": 82443989, "TTATC": 1011523, "TTATG": 28185154, "TTACA": 146122, "TTACT": 205714, "TTACC": 64537, "TTACG": 1287408, "TTAGA": 20598338, "TTAGT": 25685412, "TTAGC": 257958, "TTAGG": 10644377, "TTTAA": 60667753, "TTTAT": 87391725, "TTTAC": 887169, "TTTAG": 28417363, "TTTTA": 93923766, "TTTTT": 171067640, "TTTTC": 2967199, "TTTTG": 54426036, "TTTCA": 594863, "TTTCT": 1296473, "TTTCC": 843729, "TTTCG": 2528256, "TTTGA": 35089372, "TTTGT": 50653942, "TTTGC": 426298, "TTTGG": 18439722, "TTCAA": 394689, "TTCAT": 350149, "TTCAC": 82273, "TTCAG": 138297, "TTCTA": 312972, "TTCTT": 1076484, "TTCTC": 777328, "TTCTG": 198325, "TTCCA": 54240, "TTCCT": 651523, "TTCCC": 907986, "TTCCG": 37043, "TTCGA": 1581295, "TTCGT": 2041558, "TTCGC": 100616, "TTCGG": 1072231, "TTGAA": 25919415, "TTGAT": 32628234, "TTGAC": 287894, "TTGAG": 10633063, "TTGTA": 33253394, "TTGTT": 47917753, "TTGTC": 574454, "TTGTG": 17693744, "TTGCA": 81857, "TTGCT": 143635, "TTGCC": 48570, "TTGCG": 541853, "TTGGA": 11874146, "TTGGT": 16425134, "TTGGC": 150480, "TTGGG": 7102200, "TCAAA": 289166, "TCAAT": 220096, "TCAAC": 72251, "TCAAG": 84340, "TCATA": 180525, "TCATT": 310479, "TCATC": 46241, "TCATG": 106136, "TCACA": 19793, "TCACT": 443471, "TCACC": 34867, "TCACG": 14553, "TCAGA": 81953, "TCAGT": 114275, "TCAGC": 8471, "TCAGG": 49089, "TCTAA": 169166, "TCTAT": 265817, "TCTAC": 26795, "TCTAG": 281443, "TCTTA": 207997, "TCTTT": 1003518, "TCTTC": 681986, "TCTTG": 150714, "TCTCA": 50346, "TCTCT": 678691, "TCTCC": 934298, "TCTCG": 25469, "TCTGA": 465426, "TCTGT": 158863, "TCTGC": 22495, "TCTGG": 235513, "TCCAA": 35701, "TCCAT": 25607, "TCCAC": 17703, "TCCAG": 385128, "TCCTA": 36737, "TCCTT": 481852, "TCCTC": 826212, "TCCTG": 22419, "TCCCA": 67914, "TCCCT": 522707, "TCCCC": 930960, "TCCCG": 8092, "TCCGA": 18137, "TCCGT": 21528, "TCCGC": 5007, "TCCGG": 8532, "TCGAA": 955105, "TCGAT": 1413363, "TCGAC": 71882, "TCGAG": 708683, "TCGTA": 1255171, "TCGTT": 1982104, "TCGTC": 156239, "TCGTG": 706878, "TCGCA": 7644, "TCGCT": 11363, "TCGCC": 5014, "TCGCG": 191526, "TCGGA": 706087, "TCGGT": 912483, "TCGGC": 50093, "TCGGG": 482324, "TGAAA": 24117986, "TGAAT": 21504462, "TGAAC": 563305, "TGAAG": 9062701, "TGATA": 23944706, "TGATT": 29770646, "TGATC": 351412, "TGATG": 12993264, "TGACA": 54262, "TGACT": 78335, "TGACC": 28525, "TGACG": 523168, "TGAGA": 8239318, "TGAGT": 9793055, "TGAGC": 104196, "TGAGG": 4217806, "TGTAA": 24243161, "TGTAT": 37974162, "TGTAC": 320932, "TGTAG": 12345713, "TGTTA": 30107592, "TGTTT": 48835570, "TGTTC": 590224, "TGTTG": 17426664, "TGTCA": 126263, "TGTCT": 238568, "TGTCC": 128191, "TGTCG": 723267, "TGTGA": 12344269, "TGTGT": 21061138, "TGTGC": 150377, "TGTGG": 6549117, "TGCAA": 58038, "TGCAT": 77737, "TGCAC": 6718, "TGCAG": 30508, "TGCTA": 57496, "TGCTT": 137443, "TGCTC": 44570, "TGCTG": 34422, "TGCCA": 8769, "TGCCT": 37375, "TGCCC": 43405, "TGCCG": 20602, "TGCGA": 386070, "TGCGT": 503105, "TGCGC": 31757, "TGCGG": 213684, "TGGAA": 9561877, "TGGAT": 11212123, "TGGAC": 108450, "TGGAG": 4784514, 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"CAGAT": 73646, "CAGAC": 8388, "CAGAG": 29339, "CAGTA": 56688, "CAGTT": 102193, "CAGTC": 387284, "CAGTG": 40350, "CAGCA": 5930, "CAGCT": 7911, "CAGCC": 4569, "CAGCG": 3033, "CAGGA": 26503, "CAGGT": 40924, "CAGGC": 3413, "CAGGG": 21829, "CTAAA": 135828, "CTAAT": 117657, "CTAAC": 21007, "CTAAG": 40313, "CTATA": 152767, "CTATT": 187088, "CTATC": 395307, "CTATG": 70711, "CTACA": 14616, "CTACT": 26434, "CTACC": 13319, "CTACG": 6557, "CTAGA": 53597, "CTAGT": 165220, "CTAGC": 4853, "CTAGG": 117710, "CTTAA": 134122, "CTTAT": 158381, "CTTAC": 23531, "CTTAG": 54599, "CTTTA": 196054, "CTTTT": 949609, "CTTTC": 404885, "CTTTG": 139637, "CTTCA": 72421, "CTTCT": 459475, "CTTCC": 573301, "CTTCG": 17169, "CTTGA": 80551, "CTTGT": 124553, "CTTGC": 11148, "CTTGG": 46275, "CTCAA": 58671, "CTCAT": 33367, "CTCAC": 27550, "CTCAG": 21148, "CTCTA": 37140, "CTCTT": 415656, "CTCTC": 680362, "CTCTG": 17570, "CTCCA": 392318, "CTCCT": 501248, "CTCCC": 784241, "CTCCG": 5891, "CTCGA": 12569, "CTCGT": 17768, "CTCGC": 4091, "CTCGG": 6583, "CTGAA": 444815, "CTGAT": 62765, "CTGAC": 10050, "CTGAG": 402032, "CTGTA": 76632, "CTGTT": 127524, "CTGTC": 19741, "CTGTG": 45714, "CTGCA": 6430, "CTGCT": 21268, "CTGCC": 5035, "CTGCG": 2996, "CTGGA": 31282, "CTGGT": 116442, "CTGGC": 3509, "CTGGG": 132566, "CCAAA": 68199, "CCAAT": 22932, "CCAAC": 33725, "CCAAG": 12305, "CCATA": 14747, "CCATT": 31156, "CCATC": 19512, "CCATG": 8013, "CCACA": 12435, "CCACT": 30360, "CCACC": 23429, "CCACG": 5179, "CCAGA": 25183, "CCAGT": 384342, "CCAGC": 4397, "CCAGG": 7220, "CCTAA": 23296, "CCTAT": 21636, "CCTAC": 14128, "CCTAG": 8323, "CCTTA": 25075, "CCTTT": 320895, "CCTTC": 395571, "CCTTG": 18315, "CCTCA": 39134, "CCTCT": 464000, "CCTCC": 667908, "CCTCG": 4642, "CCTGA": 12122, "CCTGT": 16568, "CCTGC": 4446, "CCTGG": 7054, "CCCAA": 49878, "CCCAT": 22090, "CCCAC": 28192, "CCCAG": 27008, "CCCTA": 17788, "CCCTT": 245929, "CCCTC": 473650, "CCCTG": 8408, "CCCCA": 51415, "CCCCT": 290172, "CCCCC": 494651, "CCCCG": 5664, "CCCGA": 6658, "CCCGT": 7566, "CCCGC": 2524, "CCCGG": 2984, "CCGAA": 11686, "CCGAT": 12858, "CCGAC": 3612, "CCGAG": 4572, "CCGTA": 7193, "CCGTT": 25328, "CCGTC": 22679, "CCGTG": 6269, "CCGCA": 1988, "CCGCT": 3267, "CCGCC": 2394, "CCGCG": 1736, "CCGGA": 5487, "CCGGT": 7186, "CCGGC": 2416, "CCGGG": 4024, "CGAAA": 766436, "CGAAT": 765369, "CGAAC": 40739, "CGAAG": 358009, "CGATA": 1027003, "CGATT": 1214300, "CGATC": 95835, "CGATG": 435465, "CGACA": 5742, "CGACT": 8481, "CGACC": 4353, "CGACG": 151030, "CGAGA": 484982, "CGAGT": 565483, "CGAGC": 32657, "CGAGG": 251152, "CGTAA": 907283, "CGTAT": 1224503, "CGTAC": 83808, "CGTAG": 433880, "CGTTA": 1322349, "CGTTT": 1951666, "CGTTC": 137520, "CGTTG": 516095, "CGTCA": 14700, "CGTCT": 485302, "CGTCC": 14660, "CGTCG": 215960, "CGTGA": 402749, "CGTGT": 700713, "CGTGC": 35048, "CGTGG": 234041, "CGCAA": 5438, "CGCAT": 6707, "CGCAC": 2898, "CGCAG": 2856, "CGCTA": 5508, "CGCTT": 11564, "CGCTC": 5847, "CGCTG": 3066, "CGCCA": 1631, "CGCCT": 4662, "CGCCC": 4640, "CGCCG": 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--trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs/fastp/Pum1.fastp.json --html output/13-wgbs/fastp/Pum1.fastp.html " }, "PUM2 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 75145068, "total_bases": 11346905268, "q20_bases": 11206639298, "q30_bases": 10852575785, "q20_rate": 0.987638, "q30_rate": 0.956435, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.211411 }, "after_filtering": { "total_reads": 73913426, "total_bases": 10235370537, "q20_bases": 10143563017, "q30_bases": 9865171538, "q20_rate": 0.99103, "q30_rate": 0.963831, "read1_mean_length": 138, "read2_mean_length": 138, "gc_content": 0.187239 } }, "filtering_result": { "passed_filter_reads": 73913426, "low_quality_reads": 590648, "too_many_N_reads": 1460, "too_short_reads": 639534, "too_long_reads": 0 }, "duplication": { "rate": 0.0561252 }, "insert_size": { "peak": 221, 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13178, "CCAGG": 4685, "CCTAA": 10374979, "CCTAT": 8681153, "CCTAC": 4691488, "CCTAG": 63922, "CCTTA": 7503754, "CCTTT": 7144559, "CCTTC": 3498479, "CCTTG": 15671, "CCTCA": 4216405, "CCTCT": 3952317, "CCTCC": 2636255, "CCTCG": 250384, "CCTGA": 22198, "CCTGT": 17550, "CCTGC": 10840, "CCTGG": 3599, "CCCAA": 7363008, "CCCAT": 4449303, "CCCAC": 3490835, "CCCAG": 68600, "CCCTA": 5276556, "CCCTT": 3597707, "CCCTC": 2614437, "CCCTG": 15466, "CCCCA": 4108500, "CCCCT": 3123677, "CCCCC": 7054918, "CCCCG": 233702, "CCCGA": 461550, "CCCGT": 226563, "CCCGC": 175506, "CCCGG": 9367, "CCGAA": 1032970, "CCGAT": 750159, "CCGAC": 422058, "CCGAG": 8630, "CCGTA": 1253386, "CCGTT": 363178, "CCGTC": 307631, "CCGTG": 11519, "CCGCA": 213146, "CCGCT": 210343, "CCGCC": 193093, "CCGCG": 83012, "CCGGA": 8383, "CCGGT": 6432, "CCGGC": 2640, "CCGGG": 5178, "CGAAA": 2418236, "CGAAT": 1343484, "CGAAC": 860255, "CGAAG": 28724, "CGATA": 1441207, "CGATT": 899182, "CGATC": 746243, "CGATG": 5051, "CGACA": 646411, "CGACT": 469641, "CGACC": 379312, "CGACG": 215702, "CGAGA": 18139, "CGAGT": 7049, "CGAGC": 5887, "CGAGG": 10193, "CGTAA": 1257519, "CGTAT": 1650275, "CGTAC": 551550, "CGTAG": 15309, "CGTTA": 769652, "CGTTT": 671541, "CGTTC": 302793, "CGTTG": 10710, "CGTCA": 522259, "CGTCT": 396635, "CGTCC": 243771, "CGTCG": 1991032, "CGTGA": 9511, "CGTGT": 1728569, "CGTGC": 6056, "CGTGG": 45244, "CGCAA": 533431, "CGCAT": 357998, "CGCAC": 226268, "CGCAG": 8646, "CGCTA": 448881, "CGCTT": 329080, "CGCTC": 209236, "CGCTG": 2260, "CGCCA": 301328, "CGCCT": 177084, "CGCCC": 169853, "CGCCG": 779235, "CGCGA": 188366, "CGCGT": 110814, "CGCGC": 84937, "CGCGG": 3506, "CGGAA": 2471794, "CGGAT": 5442, "CGGAC": 5486, "CGGAG": 19936, "CGGTA": 6220, "CGGTT": 7395, "CGGTC": 3153, "CGGTG": 807937, "CGGCA": 4820, "CGGCT": 2345, "CGGCC": 3267, "CGGCG": 3608, "CGGGA": 29984, "CGGGT": 3882, "CGGGC": 2745, "CGGGG": 18068, "GAAAA": 8015026, "GAAAT": 3354677, "GAAAC": 1768144, "GAAAG": 5043632, "GAATA": 3159986, "GAATT": 2133224, 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66872, "GTGGT": 820273, "GTGGC": 19024, "GTGGG": 105243, "GCAAA": 721195, "GCAAT": 418550, "GCAAC": 244696, "GCAAG": 10833, "GCATA": 403179, "GCATT": 279963, "GCATC": 167769, "GCATG": 3534, "GCACA": 222566, "GCACT": 155708, "GCACC": 100275, "GCACG": 40125, "GCAGA": 21849, "GCAGT": 7206, "GCAGC": 4267, "GCAGG": 10588, "GCTAA": 455608, "GCTAT": 377754, "GCTAC": 201945, "GCTAG": 4107, "GCTTA": 288801, "GCTTT": 255407, "GCTTC": 160939, "GCTTG": 2463, "GCTCA": 194486, "GCTCT": 269527, "GCTCC": 91734, "GCTCG": 43573, "GCTGA": 3926, "GCTGT": 2682, "GCTGC": 2062, "GCTGG": 2700, "GCCAA": 281435, "GCCAT": 245664, "GCCAC": 124345, "GCCAG": 5045, "GCCTA": 175475, "GCCTT": 127237, "GCCTC": 86529, "GCCTG": 2183, "GCCCA": 128191, "GCCCT": 99716, "GCCCC": 105056, "GCCCG": 35449, "GCCGA": 60041, "GCCGT": 740297, "GCCGC": 22772, "GCCGG": 2679, "GCGAA": 126813, "GCGAT": 78913, "GCGAC": 48378, "GCGAG": 11893, "GCGTA": 73050, "GCGTT": 43569, "GCGTC": 1923337, "GCGTG": 10961, "GCGCA": 38225, "GCGCT": 27556, 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"GAGGA": 571350, "GAGGT": 46991, "GAGGC": 37603, "GAGGG": 308603, "GTAAA": 779487, "GTAAT": 460902, "GTAAC": 287676, "GTAAG": 8361, "GTATA": 542477, "GTATT": 417555, "GTATC": 561361, "GTATG": 3236, "GTACA": 297324, "GTACT": 173182, "GTACC": 120893, "GTACG": 75074, "GTAGA": 333917, "GTAGT": 3591, "GTAGC": 1676, "GTAGG": 360276, "GTTAA": 413331, "GTTAT": 352011, "GTTAC": 188187, "GTTAG": 2403, "GTTTA": 358030, "GTTTT": 324332, "GTTTC": 177206, "GTTTG": 4112, "GTTCA": 182313, "GTTCT": 123713, "GTTCC": 88140, "GTTCG": 32451, "GTTGA": 2822, "GTTGT": 5852, "GTTGC": 10218, "GTTGG": 2976, "GTCAA": 268126, "GTCAT": 250551, "GTCAC": 152843, "GTCAG": 1895, "GTCTA": 216559, "GTCTT": 143064, "GTCTC": 105090, "GTCTG": 2053, "GTCCA": 103553, "GTCCT": 80015, "GTCCC": 69117, "GTCCG": 56526, "GTCGA": 95121, "GTCGT": 423168, "GTCGC": 372599, "GTCGG": 6658, "GTGAA": 10103, "GTGAT": 3640, "GTGAC": 358593, "GTGAG": 7414, "GTGTA": 674870, "GTGTT": 7215, "GTGTC": 38121, "GTGTG": 365646, "GTGCA": 1951, 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2078, "GCGCA": 29146, "GCGCT": 22301, "GCGCC": 21725, "GCGCG": 15314, "GCGGA": 2070, "GCGGT": 785, "GCGGC": 835, "GCGGG": 2121, "GGAAA": 988008, "GGAAT": 343945, "GGAAC": 147944, "GGAAG": 385772, "GGATA": 350165, "GGATT": 256130, "GGATC": 136290, "GGATG": 16710, "GGACA": 142805, "GGACT": 99321, "GGACC": 61799, "GGACG": 7504, "GGAGA": 544545, "GGAGT": 47175, "GGAGC": 37870, "GGAGG": 329666, "GGTAA": 61920, "GGTAT": 41750, "GGTAC": 25757, "GGTAG": 7809, "GGTTA": 42304, "GGTTT": 35821, "GGTTC": 19368, "GGTTG": 12473, "GGTCA": 30073, "GGTCT": 15540, "GGTCC": 11507, "GGTCG": 352670, "GGTGA": 7272, "GGTGT": 3839, "GGTGC": 1497, "GGTGG": 367265, "GGCAA": 51661, "GGCAT": 32084, "GGCAC": 18281, "GGCAG": 2173, "GGCTA": 42625, "GGCTT": 26210, "GGCTC": 15831, "GGCTG": 1273, "GGCCA": 23528, "GGCCT": 13524, "GGCCC": 12476, "GGCCG": 2128, "GGCGA": 3918, "GGCGT": 2367, "GGCGC": 1779, "GGCGG": 2234, "GGGAA": 756564, "GGGAT": 195252, "GGGAC": 75715, "GGGAG": 313401, "GGGTA": 37329, "GGGTT": 27445, "GGGTC": 14902, "GGGTG": 12873, "GGGCA": 26302, "GGGCT": 21701, "GGGCC": 12964, "GGGCG": 3312, "GGGGA": 330196, "GGGGT": 30526, "GGGGC": 16890, "GGGGG": 1208908 }, "overrepresented_sequences": {} }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/PUM2 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/PUM2 (paired)_R2.fastq.gz -o output/13-wgbs/trimmed/Pum2_R1.fq.gz -O output/13-wgbs/trimmed/Pum2_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs/fastp/Pum2.fastp.json --html output/13-wgbs/fastp/Pum2.fastp.html " }, "PUM3 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 65100878, "total_bases": 9830232578, "q20_bases": 9708703714, "q30_bases": 9401934736, "q20_rate": 0.987637, "q30_rate": 0.956431, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.211413 }, "after_filtering": { "total_reads": 64034112, "total_bases": 8867293350, "q20_bases": 8787737632, "q30_bases": 8546539604, "q20_rate": 0.991028, "q30_rate": 0.963827, "read1_mean_length": 138, "read2_mean_length": 138, "gc_content": 0.187238 } }, "filtering_result": { "passed_filter_reads": 64034112, "low_quality_reads": 511638, "too_many_N_reads": 1276, "too_short_reads": 553852, "too_long_reads": 0 }, "duplication": { "rate": 0.0492082 }, "insert_size": { "peak": 225, "unknown": 917871, "histogram": [ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 2357, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 358, 331, 323, 337, 322, 289, 332, 310, 295, 315, 300, 306, 317, 309, 332, 328, 311, 322, 344, 312, 331, 306, 343, 349, 382, 385, 379, 372, 446, 393, 418, 419, 433, 490, 466, 484, 532, 503, 513, 513, 522, 561, 654, 597, 609, 659, 712, 727, 774, 708, 861, 771, 898, 863, 911, 978, 974, 1126, 1123, 1152, 1205, 1319, 1286, 1457, 1472, 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35855498, "TTTTT": 33359036, "TTTTC": 17028334, "TTTTG": 64834, "TTTCA": 20365451, "TTTCT": 14429571, "TTTCC": 7640333, "TTTCG": 628387, "TTTGA": 69292, "TTTGT": 50219, "TTTGC": 21408, "TTTGG": 13561, "TTCAA": 22245494, "TTCAT": 16358397, "TTCAC": 8255603, "TTCAG": 67232, "TTCTA": 14572435, "TTCTT": 12074612, "TTCTC": 6364068, "TTCTG": 28437, "TTCCA": 8157534, "TTCCT": 6111208, "TTCCC": 3497024, "TTCCG": 490108, "TTCGA": 793087, "TTCGT": 533923, "TTCGC": 267191, "TTCGG": 6128, "TTGAA": 81293, "TTGAT": 53752, "TTGAC": 30959, "TTGAG": 7131, "TTGTA": 44354, "TTGTT": 50953, "TTGTC": 30609, "TTGTG": 11975, "TTGCA": 24770, "TTGCT": 17609, "TTGCC": 35676, "TTGCG": 3209, "TTGGA": 10828, "TTGGT": 7686, "TTGGC": 2929, "TTGGG": 17103, "TCAAA": 30439733, "TCAAT": 19337433, "TCAAC": 10197040, "TCAAG": 60106, "TCATA": 17950278, "TCATT": 16096248, "TCATC": 7305114, "TCATG": 32000, "TCACA": 10707532, "TCACT": 8000445, "TCACC": 4392330, "TCACG": 332822, "TCAGA": 152004, "TCAGT": 45197, "TCAGC": 11850, 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17184, "CGGTA": 5368, "CGGTT": 6388, "CGGTC": 2695, "CGGTG": 699797, "CGGCA": 4163, "CGGCT": 2033, "CGGCC": 2861, "CGGCG": 3106, "CGGGA": 25948, "CGGGT": 3360, "CGGGC": 2394, "CGGGG": 15662, "GAAAA": 6941791, "GAAAT": 2907406, "GAAAC": 1531173, "GAAAG": 4369637, "GAATA": 2737266, "GAATT": 1847630, "GAATC": 1123553, "GAATG": 40862, "GAACA": 1416834, "GAACT": 853399, "GAACC": 547744, "GAACG": 158275, "GAAGA": 8176128, "GAAGT": 281201, "GAAGC": 262207, "GAAGG": 4379020, "GATAA": 2524014, "GATAT": 1766270, "GATAC": 1007481, "GATAG": 55284, "GATTA": 1610344, "GATTT": 1319453, "GATTC": 682826, "GATTG": 9813, "GATCA": 1112804, "GATCT": 1469144, "GATCC": 398437, "GATCG": 2359270, "GATGA": 48097, "GATGT": 10048, "GATGC": 5044, "GATGG": 50379, "GACAA": 2214480, "GACAT": 782387, "GACAC": 448804, "GACAG": 12319, "GACTA": 862648, "GACTT": 543974, "GACTC": 317340, "GACTG": 3484, "GACCA": 543374, "GACCT": 313521, "GACCC": 237078, "GACCG": 77656, "GACGA": 147974, "GACGT": 131079, "GACGC": 55037, 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86413, "GTCCG": 53650, "GTCGA": 133232, "GTCGT": 1624443, "GTCGC": 665792, "GTCGG": 25937, "GTGAA": 53221, "GTGAT": 6778, "GTGAC": 949416, "GTGAG": 33747, "GTGTA": 2094944, "GTGTT": 20806, "GTGTC": 97994, "GTGTG": 1112737, "GTGCA": 6607, "GTGCT": 97838, "GTGCC": 5457, "GTGCG": 3174, "GTGGA": 57763, "GTGGT": 710546, "GTGGC": 16536, "GTGGG": 90883, "GCAAA": 625317, "GCAAT": 362282, "GCAAC": 211605, "GCAAG": 9409, "GCATA": 349618, "GCATT": 242641, "GCATC": 145299, "GCATG": 3025, "GCACA": 192737, "GCACT": 134940, "GCACC": 86944, "GCACG": 34780, "GCAGA": 18963, "GCAGT": 6267, "GCAGC": 3687, "GCAGG": 9181, "GCTAA": 395378, "GCTAT": 327194, "GCTAC": 175179, "GCTAG": 3563, "GCTTA": 250561, "GCTTT": 221602, "GCTTC": 139393, "GCTTG": 2131, "GCTCA": 168433, "GCTCT": 233610, "GCTCC": 79600, "GCTCG": 37881, "GCTGA": 3382, "GCTGT": 2287, "GCTGC": 1798, "GCTGG": 2326, "GCCAA": 243875, "GCCAT": 212682, "GCCAC": 107903, "GCCAG": 4377, "GCCTA": 152246, "GCCTT": 110151, "GCCTC": 74879, "GCCTG": 1846, 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322367, "TCAGA": 20609, "TCAGT": 24754, "TCAGC": 7599, "TCAGG": 1961, "TCTAA": 17110845, "TCTAT": 15576351, "TCTAC": 7636012, "TCTAG": 27202, "TCTTA": 11283596, "TCTTT": 11663206, "TCTTC": 6235605, "TCTTG": 17142, "TCTCA": 6783516, "TCTCT": 9735457, "TCTCC": 3364984, "TCTCG": 689977, "TCTGA": 41953, "TCTGT": 26724, "TCTGC": 10673, "TCTGG": 63582, "TCCAA": 9943878, "TCCAT": 7359069, "TCCAC": 4066164, "TCCAG": 12034, "TCCTA": 6275238, "TCCTT": 5044523, "TCCTC": 3164515, "TCCTG": 10854, "TCCCA": 3932380, "TCCCT": 2879941, "TCCCC": 2215945, "TCCCG": 197476, "TCCGA": 638811, "TCCGT": 401711, "TCCGC": 175281, "TCCGG": 1221, "TCGAA": 1281100, "TCGAT": 854107, "TCGAC": 443522, "TCGAG": 2136, "TCGTA": 753323, "TCGTT": 489047, "TCGTC": 320962, "TCGTG": 314869, "TCGCA": 316815, "TCGCT": 292639, "TCGCC": 483624, "TCGCG": 97789, "TCGGA": 21599, "TCGGT": 309861, "TCGGC": 1241, "TCGGG": 5227, "TGAAA": 130108, "TGAAT": 70040, "TGAAC": 36495, "TGAAG": 10641, "TGATA": 78510, "TGATT": 52750, "TGATC": 27826, "TGATG": 2300, "TGACA": 350450, "TGACT": 22873, "TGACC": 22839, "TGACG": 2875, "TGAGA": 14744, "TGAGT": 3255, "TGAGC": 1446, "TGAGG": 17933, "TGTAA": 62248, "TGTAT": 59132, "TGTAC": 25714, "TGTAG": 589677, "TGTTA": 43006, "TGTTT": 53404, "TGTTC": 28662, "TGTTG": 3269, "TGTCA": 31221, "TGTCT": 22618, "TGTCC": 16148, "TGTCG": 34906, "TGTGA": 313364, "TGTGT": 4819, "TGTGC": 5730, "TGTGG": 6473, "TGCAA": 30477, "TGCAT": 25805, "TGCAC": 14379, "TGCAG": 1585, "TGCTA": 18729, "TGCTT": 16631, "TGCTC": 22960, "TGCTG": 1189, "TGCCA": 12546, "TGCCT": 8712, "TGCCC": 7850, "TGCCG": 8127, "TGCGA": 2172, "TGCGT": 1754, "TGCGC": 2028, "TGCGG": 759, "TGGAA": 16367, "TGGAT": 3962, "TGGAC": 1880, "TGGAG": 25750, "TGGTA": 1931, "TGGTT": 10657, "TGGTC": 306970, "TGGTG": 2811, "TGGCA": 1950, "TGGCT": 1371, "TGGCC": 1282, "TGGCG": 784, "TGGGA": 30665, "TGGGT": 2852, "TGGGC": 1461, "TGGGG": 40808, "CAAAA": 45990184, "CAAAT": 27165761, "CAAAC": 15098420, "CAAAG": 43444, "CAATA": 28306790, "CAATT": 17834721, "CAATC": 9245961, "CAATG": 47035, "CAACA": 14906263, "CAACT": 8723130, "CAACC": 5356719, "CAACG": 424949, "CAAGA": 31914, "CAAGT": 37933, "CAAGC": 11643, "CAAGG": 2759, "CATAA": 23756814, "CATAT": 21842061, "CATAC": 10856744, "CATAG": 23579, "CATTA": 18515482, "CATTT": 17990743, "CATTC": 6857943, "CATTG": 25648, "CATCA": 10901290, "CATCT": 6694938, "CATCC": 3769540, "CATCG": 356146, "CATGA": 42168, "CATGT": 33676, "CATGC": 15146, "CATGG": 1487, "CACAA": 14983071, "CACAT": 10519154, "CACAC": 8397327, "CACAG": 14473, "CACTA": 10502461, "CACTT": 6803818, "CACTC": 4594947, "CACTG": 17197, "CACCA": 6213492, "CACCT": 4195087, "CACCC": 2726344, "CACCG": 262202, "CACGA": 563228, "CACGT": 356696, "CACGC": 182669, "CACGG": 1268, "CAGAA": 30243, "CAGAT": 30672, "CAGAC": 13347, "CAGAG": 2904, "CAGTA": 18301, "CAGTT": 16552, "CAGTC": 11638, "CAGTG": 13718, "CAGCA": 11207, "CAGCT": 8050, "CAGCC": 7139, "CAGCG": 1133, "CAGGA": 3054, "CAGGT": 1348, "CAGGC": 1235, "CAGGG": 2169, "CTAAA": 23790187, "CTAAT": 15318196, "CTAAC": 8662130, "CTAAG": 18920, "CTATA": 20611525, "CTATT": 13148818, "CTATC": 7586955, "CTATG": 30607, "CTACA": 10403661, "CTACT": 6330288, "CTACC": 3768376, "CTACG": 352185, "CTAGA": 22934, "CTAGT": 14577, "CTAGC": 6354, "CTAGG": 10614, "CTTAA": 14762870, "CTTAT": 11216976, "CTTAC": 6246772, "CTTAG": 11823, "CTTTA": 14244284, "CTTTT": 12111267, "CTTTC": 6607207, "CTTTG": 20062, "CTTCA": 7543998, "CTTCT": 5280900, "CTTCC": 3315779, "CTTCG": 290879, "CTTGA": 23241, "CTTGT": 14919, "CTTGC": 7274, "CTTGG": 1500, "CTCAA": 8905015, "CTCAT": 5535579, "CTCAC": 3827292, "CTCAG": 13017, "CTCTA": 7734163, "CTCTT": 4965746, "CTCTC": 7096111, "CTCTG": 16952, "CTCCA": 3935744, "CTCCT": 2792677, "CTCCC": 1956743, "CTCCG": 197091, "CTCGA": 573818, "CTCGT": 297786, "CTCGC": 204009, "CTCGG": 309870, "CTGAA": 41648, "CTGAT": 27572, "CTGAC": 15030, "CTGAG": 13147, "CTGTA": 27324, "CTGTT": 21277, "CTGTC": 13297, "CTGTG": 3250, "CTGCA": 13032, "CTGCT": 9082, "CTGCC": 6043, 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"CCGTC": 247295, "CCGTG": 2429, "CCGCA": 174773, "CCGCT": 172950, "CCGCC": 153291, "CCGCG": 68072, "CCGGA": 2267, "CCGGT": 1433, "CCGGC": 950, "CCGGG": 783, "CGAAA": 2011483, "CGAAT": 1129311, "CGAAC": 717135, "CGAAG": 4921, "CGATA": 1213397, "CGATT": 757875, "CGATC": 571744, "CGATG": 3019, "CGACA": 538059, "CGACT": 393892, "CGACC": 310575, "CGACG": 178438, "CGAGA": 3608, "CGAGT": 2115, "CGAGC": 1445, "CGAGG": 1669, "CGTAA": 1058746, "CGTAT": 1106527, "CGTAC": 459824, "CGTAG": 2387, "CGTTA": 647175, "CGTTT": 563639, "CGTTC": 252638, "CGTTG": 2494, "CGTCA": 436544, "CGTCT": 321272, "CGTCC": 199971, "CGTCG": 433278, "CGTGA": 3048, "CGTGT": 316657, "CGTGC": 1650, "CGTGG": 2186, "CGCAA": 443716, "CGCAT": 299164, "CGCAC": 184375, "CGCAG": 1954, "CGCTA": 377337, "CGCTT": 275014, "CGCTC": 168579, "CGCTG": 1192, "CGCCA": 232785, "CGCCT": 144131, "CGCCC": 133181, "CGCCG": 364227, "CGCGA": 156866, "CGCGT": 88320, "CGCGC": 69225, "CGCGG": 760, "CGGAA": 24145, "CGGAT": 1925, "CGGAC": 1588, 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663432, "GAGAT": 324076, "GAGAC": 135583, "GAGAG": 427514, "GAGTA": 64482, "GAGTT": 44613, "GAGTC": 25614, "GAGTG": 309788, "GAGCA": 50827, "GAGCT": 39394, "GAGCC": 23038, "GAGCG": 115527, "GAGGA": 495020, "GAGGT": 40650, "GAGGC": 32665, "GAGGG": 267458, "GTAAA": 675242, "GTAAT": 399247, "GTAAC": 249283, "GTAAG": 7301, "GTATA": 470042, "GTATT": 361573, "GTATC": 486861, "GTATG": 2806, "GTACA": 257794, "GTACT": 149727, "GTACC": 104858, "GTACG": 64934, "GTAGA": 289412, "GTAGT": 3124, "GTAGC": 1415, "GTAGG": 312363, "GTTAA": 358173, "GTTAT": 304777, "GTTAC": 163475, "GTTAG": 2109, "GTTTA": 310180, "GTTTT": 280420, "GTTTC": 153411, "GTTTG": 3572, "GTTCA": 157941, "GTTCT": 107174, "GTTCC": 76432, "GTTCG": 28220, "GTTGA": 2442, "GTTGT": 5021, "GTTGC": 8830, "GTTGG": 2553, "GTCAA": 232309, "GTCAT": 217702, "GTCAC": 132297, "GTCAG": 1640, "GTCTA": 187687, "GTCTT": 123651, "GTCTC": 90823, "GTCTG": 1748, "GTCCA": 89660, "GTCCT": 69369, "GTCCC": 59641, "GTCCG": 48890, "GTCGA": 82610, "GTCGT": 366845, "GTCGC": 322952, "GTCGG": 5720, "GTGAA": 8736, "GTGAT": 3153, "GTGAC": 310876, "GTGAG": 6417, "GTGTA": 585002, "GTGTT": 6268, "GTGTC": 33041, "GTGTG": 316925, "GTGCA": 1689, "GTGCT": 9163, "GTGCC": 1511, "GTGCG": 798, "GTGGA": 7317, "GTGGT": 315913, "GTGGC": 1758, "GTGGG": 11817, "GCAAA": 323850, "GCAAT": 200999, "GCAAC": 115399, "GCAAG": 2260, "GCATA": 203293, "GCATT": 145279, "GCATC": 84108, "GCATG": 1419, "GCACA": 108796, "GCACT": 77852, "GCACC": 47009, "GCACG": 28677, "GCAGA": 2803, "GCAGT": 2301, "GCAGC": 1369, "GCAGG": 1867, "GCTAA": 229443, "GCTAT": 194160, "GCTAC": 98006, "GCTAG": 809, "GCTTA": 145084, "GCTTT": 140568, "GCTTC": 88072, "GCTTG": 1017, "GCTCA": 94689, "GCTCT": 86277, "GCTCC": 42747, "GCTCG": 27127, "GCTGA": 1472, "GCTGT": 1154, "GCTGC": 930, "GCTGG": 1136, "GCCAA": 132185, "GCCAT": 123559, "GCCAC": 62737, "GCCAG": 1251, "GCCTA": 92013, "GCCTT": 63034, "GCCTC": 41850, "GCCTG": 1153, "GCCCA": 62572, "GCCCT": 47491, "GCCCC": 51595, "GCCCG": 24079, "GCCGA": 41516, "GCCGT": 320747, "GCCGC": 15116, "GCCGG": 749, "GCGAA": 79382, "GCGAT": 56909, "GCGAC": 32181, "GCGAG": 1521, "GCGTA": 47885, "GCGTT": 29602, "GCGTC": 330720, "GCGTG": 1796, "GCGCA": 25205, "GCGCT": 19339, "GCGCC": 18759, "GCGCG": 13306, "GCGGA": 1806, "GCGGT": 684, "GCGGC": 713, "GCGGG": 1843, "GGAAA": 855135, "GGAAT": 298057, "GGAAC": 128418, "GGAAG": 334345, "GGATA": 303512, "GGATT": 221869, "GGATC": 118228, "GGATG": 14480, "GGACA": 123720, "GGACT": 85818, "GGACC": 53619, "GGACG": 6524, "GGAGA": 471337, "GGAGT": 40811, "GGAGC": 32710, "GGAGG": 285851, "GGTAA": 53680, "GGTAT": 36184, "GGTAC": 22271, "GGTAG": 6718, "GGTTA": 36773, "GGTTT": 31161, "GGTTC": 16722, "GGTTG": 10755, "GGTCA": 25988, "GGTCT": 13448, "GGTCC": 10028, "GGTCG": 305754, "GGTGA": 6255, "GGTGT": 3325, "GGTGC": 1301, "GGTGG": 318420, "GGCAA": 44540, "GGCAT": 27733, "GGCAC": 15866, "GGCAG": 1907, "GGCTA": 36828, "GGCTT": 22821, "GGCTC": 13791, "GGCTG": 1105, "GGCCA": 20337, "GGCCT": 11747, "GGCCC": 10790, "GGCCG": 1856, "GGCGA": 3393, "GGCGT": 2043, "GGCGC": 1533, "GGCGG": 1963, "GGGAA": 655560, "GGGAT": 169212, "GGGAC": 65430, "GGGAG": 271413, "GGGTA": 32317, "GGGTT": 23933, "GGGTC": 12929, "GGGTG": 11153, "GGGCA": 22681, "GGGCT": 18854, "GGGCC": 11176, "GGGCG": 2902, "GGGGA": 286289, "GGGGT": 26521, "GGGGC": 14565, "GGGGG": 1048849 }, "overrepresented_sequences": {} }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/PUM3 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/PUM3 (paired)_R2.fastq.gz -o output/13-wgbs/trimmed/Pum3_R1.fq.gz -O output/13-wgbs/trimmed/Pum3_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs/fastp/Pum3.fastp.json --html output/13-wgbs/fastp/Pum3.fastp.html " }, "PUM4 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 61300626, "total_bases": 9256394526, "q20_bases": 9142940677, "q30_bases": 8858100751, "q20_rate": 0.987743, "q30_rate": 0.956971, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.212189 }, "after_filtering": { "total_reads": 60312596, "total_bases": 8342932180, "q20_bases": 8269142285, "q30_bases": 8046290019, "q20_rate": 0.991155, "q30_rate": 0.964444, "read1_mean_length": 138, "read2_mean_length": 138, "gc_content": 0.18781 } }, "filtering_result": { "passed_filter_reads": 60312596, "low_quality_reads": 480886, "too_many_N_reads": 1238, "too_short_reads": 505906, "too_long_reads": 0 }, "duplication": { "rate": 0.03101 }, "insert_size": { "peak": 190, "unknown": 794037, "histogram": [ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 2255, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 318, 331, 356, 288, 296, 318, 272, 273, 301, 299, 288, 292, 277, 260, 306, 265, 308, 265, 322, 293, 312, 341, 360, 333, 323, 336, 368, 360, 378, 391, 425, 419, 408, 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34149, "TTGCG": 3135, "TTGGA": 11369, "TTGGT": 7907, "TTGGC": 3230, "TTGGG": 16932, "TCAAA": 28817297, "TCAAT": 18182093, "TCAAC": 9683817, "TCAAG": 64034, "TCATA": 16889004, "TCATT": 14997780, "TCATC": 6869800, "TCATG": 33370, "TCACA": 10120658, "TCACT": 7468811, "TCACC": 4174736, "TCACG": 315006, "TCAGA": 159370, "TCAGT": 87189, "TCAGC": 12318, "TCAGG": 5935, "TCTAA": 16467337, "TCTAT": 14764516, "TCTAC": 7347708, "TCTAG": 103012, "TCTTA": 10783155, "TCTTT": 11041115, "TCTTC": 6073459, "TCTTG": 22359, "TCTCA": 6574620, "TCTCT": 9184051, "TCTCC": 3320902, "TCTCG": 1050937, "TCTGA": 49739, "TCTGT": 40174, "TCTGC": 14057, "TCTGG": 65452, "TCCAA": 9709713, "TCCAT": 7099267, "TCCAC": 4007910, "TCCAG": 44904, "TCCTA": 6082523, "TCCTT": 4880710, "TCCTC": 3131069, "TCCTG": 14254, "TCCCA": 3907815, "TCCCT": 2850480, "TCCCC": 2387148, "TCCCG": 198761, "TCCGA": 650875, "TCCGT": 398576, "TCCGC": 174139, "TCCGG": 3130, "TCGAA": 1262933, "TCGAT": 865477, "TCGAC": 439567, "TCGAG": 5438, "TCGTA": 732426, "TCGTT": 473231, "TCGTC": 316533, "TCGTG": 1534034, "TCGCA": 311807, "TCGCT": 285300, "TCGCC": 777295, "TCGCG": 96575, "TCGGA": 2202159, "TCGGT": 675098, "TCGGC": 3668, "TCGGG": 22748, "TGAAA": 179251, "TGAAT": 81381, "TGAAC": 44414, "TGAAG": 29292, "TGATA": 88830, "TGATT": 60699, "TGATC": 32350, "TGATG": 4948, "TGACA": 697621, "TGACT": 26937, "TGACC": 29608, "TGACG": 4438, "TGAGA": 37171, "TGAGT": 9430, "TGAGC": 3818, "TGAGG": 47479, "TGTAA": 344114, "TGTAT": 67032, "TGTAC": 31909, "TGTAG": 2032036, "TGTTA": 53507, "TGTTT": 64369, "TGTTC": 34468, "TGTTG": 12140, "TGTCA": 62614, "TGTCT": 27999, "TGTCC": 21007, "TGTCG": 100361, "TGTGA": 700663, "TGTGT": 13861, "TGTGC": 72535, "TGTGG": 42877, "TGCAA": 38084, "TGCAT": 29803, "TGCAC": 18587, "TGCAG": 6100, "TGCTA": 22383, "TGCTT": 20071, "TGCTC": 105118, "TGCTG": 2861, "TGCCA": 18985, "TGCCT": 11816, "TGCCC": 13780, "TGCCG": 21538, "TGCGA": 4019, "TGCGT": 3025, "TGCGC": 3385, "TGCGG": 2492, "TGGAA": 49546, "TGGAT": 8532, "TGGAC": 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556371, "CACGT": 349954, "CACGC": 183080, "CACGG": 2911, "CAGAA": 48761, "CAGAT": 381860, "CAGAC": 39359, "CAGAG": 12965, "CAGTA": 25030, "CAGTT": 22751, "CAGTC": 14724, "CAGTG": 73386, "CAGCA": 17543, "CAGCT": 11553, "CAGCC": 13381, "CAGCG": 3186, "CAGGA": 11483, "CAGGT": 3665, "CAGGC": 3522, "CAGGG": 9632, "CTAAA": 23107911, "CTAAT": 14704210, "CTAAC": 8433837, "CTAAG": 109514, "CTATA": 19808487, "CTATT": 12511099, "CTATC": 7305075, "CTATG": 34777, "CTACA": 10140728, "CTACT": 6104473, "CTACC": 3704081, "CTACG": 342853, "CTAGA": 214713, "CTAGT": 17190, "CTAGC": 8889, "CTAGG": 12796, "CTTAA": 14244132, "CTTAT": 10676034, "CTTAC": 6020355, "CTTAG": 80535, "CTTTA": 13584653, "CTTTT": 11508489, "CTTTC": 6372253, "CTTTG": 25380, "CTTCA": 7329923, "CTTCT": 5108651, "CTTCC": 3349949, "CTTCG": 282471, "CTTGA": 27733, "CTTGT": 19347, "CTTGC": 9635, "CTTGG": 4512, "CTCAA": 8729086, "CTCAT": 5355650, "CTCAC": 3781866, "CTCAG": 58235, "CTCTA": 7482108, "CTCTT": 4864176, "CTCTC": 6728453, "CTCTG": 28184, "CTCCA": 3893177, "CTCCT": 2779119, "CTCCC": 2117378, "CTCCG": 202305, "CTCGA": 561926, "CTCGT": 291821, "CTCGC": 201073, "CTCGG": 682983, "CTGAA": 51362, "CTGAT": 31739, "CTGAC": 18311, "CTGAG": 18055, "CTGTA": 34108, "CTGTT": 26704, "CTGTC": 16613, "CTGTG": 10856, "CTGCA": 17139, "CTGCT": 11765, "CTGCC": 9414, "CTGCG": 2324, "CTGGA": 22458, "CTGGT": 2458, "CTGGC": 2215, "CTGGG": 48064, "CCAAA": 15534126, "CCAAT": 9051962, "CCAAC": 5239242, "CCAAG": 53526, "CCATA": 9483421, "CCATT": 6944808, "CCATC": 3773103, "CCATG": 24512, "CCACA": 5623851, "CCACT": 3519840, "CCACC": 3311441, "CCACG": 196963, "CCAGA": 124996, "CCAGT": 16836, "CCAGC": 11609, "CCAGG": 4660, "CCTAA": 8546725, "CCTAT": 7075048, "CCTAC": 3854345, "CCTAG": 56991, "CCTTA": 6093164, "CCTTT": 5772440, "CCTTC": 2860997, "CCTTG": 13994, "CCTCA": 3472877, "CCTCT": 3214094, "CCTCC": 2157175, "CCTCG": 205134, "CCTGA": 20946, "CCTGT": 15623, "CCTGC": 9682, "CCTGG": 3692, "CCCAA": 6106832, "CCCAT": 3648501, "CCCAC": 2885141, "CCCAG": 58974, "CCCTA": 4368056, "CCCTT": 2937401, "CCCTC": 2136473, "CCCTG": 13871, "CCCCA": 3396563, "CCCCT": 2569126, "CCCCC": 5878188, "CCCCG": 194468, "CCCGA": 383222, "CCCGT": 186200, "CCCGC": 143912, "CCCGG": 7688, "CCGAA": 858668, "CCGAT": 615663, "CCGAC": 346009, "CCGAG": 7169, "CCGTA": 1025081, "CCGTT": 296299, "CCGTC": 253401, "CCGTG": 10040, "CCGCA": 174863, "CCGCT": 170084, "CCGCC": 159711, "CCGCG": 68989, "CCGGA": 7231, "CCGGT": 5239, "CCGGC": 2324, "CCGGG": 4292, "CGAAA": 1999390, "CGAAT": 1103780, "CGAAC": 713009, "CGAAG": 24660, "CGATA": 1181292, "CGATT": 731904, "CGATC": 616482, "CGATG": 4578, "CGACA": 534760, "CGACT": 383756, "CGACC": 312577, "CGACG": 179722, "CGAGA": 15368, "CGAGT": 5674, "CGAGC": 4925, "CGAGG": 8135, "CGTAA": 1029418, "CGTAT": 1341138, "CGTAC": 450670, "CGTAG": 13008, "CGTTA": 624198, "CGTTT": 541711, "CGTTC": 246756, "CGTTG": 9390, "CGTCA": 423823, "CGTCT": 324872, "CGTCC": 199887, "CGTCG": 1740052, "CGTGA": 7955, "CGTGT": 1504442, "CGTGC": 5151, "CGTGG": 40813, "CGCAA": 438717, "CGCAT": 292082, "CGCAC": 185453, "CGCAG": 7249, "CGCTA": 367868, "CGCTT": 264986, "CGCTC": 172669, "CGCTG": 2021, "CGCCA": 249522, "CGCCT": 144594, "CGCCC": 141657, "CGCCG": 637041, "CGCGA": 155979, "CGCGT": 90547, "CGCGC": 70109, "CGCGG": 2876, "CGGAA": 2195706, "CGGAT": 4709, "CGGAC": 4517, "CGGAG": 16591, "CGGTA": 5170, "CGGTT": 6270, "CGGTC": 2694, "CGGTG": 663144, "CGGCA": 3955, "CGGCT": 2028, "CGGCC": 2853, "CGGCG": 3046, "CGGGA": 25143, "CGGGT": 3197, "CGGGC": 2436, "CGGGG": 14975, "GAAAA": 6511945, "GAAAT": 2728243, "GAAAC": 1458380, "GAAAG": 4141527, "GAATA": 2545967, "GAATT": 1715902, "GAATC": 1087919, "GAATG": 39559, "GAACA": 1349283, "GAACT": 804766, "GAACC": 540774, "GAACG": 152468, "GAAGA": 7826600, "GAAGT": 267325, "GAAGC": 247381, "GAAGG": 4073969, "GATAA": 2374035, "GATAT": 1633034, "GATAC": 953728, "GATAG": 50960, "GATTA": 1515061, "GATTT": 1204894, "GATTC": 647988, "GATTG": 10382, "GATCA": 1103977, "GATCT": 1399531, "GATCC": 397242, "GATCG": 2432902, "GATGA": 45141, "GATGT": 10187, "GATGC": 5170, "GATGG": 46491, "GACAA": 1886228, "GACAT": 739922, "GACAC": 431062, "GACAG": 12305, "GACTA": 806458, "GACTT": 502834, "GACTC": 302375, "GACTG": 3978, "GACCA": 544451, "GACCT": 309046, "GACCC": 236906, "GACCG": 76209, "GACGA": 142365, "GACGT": 124657, "GACGC": 53452, "GACGG": 7996, "GAGAA": 7190732, "GAGAT": 2288707, "GAGAC": 1055438, "GAGAG": 7478480, "GAGTA": 391768, "GAGTT": 255430, "GAGTC": 189776, "GAGTG": 1046413, "GAGCA": 338674, "GAGCT": 257509, "GAGCC": 170219, "GAGCG": 1799015, "GAGGA": 8953424, "GAGGT": 457451, "GAGGC": 429446, "GAGGG": 5561146, "GTAAA": 924659, "GTAAT": 777751, "GTAAC": 345609, "GTAAG": 28472, "GTATA": 599819, "GTATT": 440746, "GTATC": 798579, "GTATG": 7299, "GTACA": 370150, "GTACT": 207973, "GTACC": 141022, "GTACG": 69452, "GTAGA": 712242, "GTAGT": 9172, "GTAGC": 4128, "GTAGG": 1325730, "GTTAA": 483477, "GTTAT": 396058, "GTTAC": 220994, "GTTAG": 8780, "GTTTA": 389600, "GTTTT": 347691, "GTTTC": 198005, "GTTTG": 11480, "GTTCA": 229562, "GTTCT": 149867, "GTTCC": 103153, "GTTCG": 31291, "GTTGA": 6625, "GTTGT": 20235, "GTTGC": 26905, "GTTGG": 11160, "GTCAA": 363902, "GTCAT": 313972, "GTCAC": 188513, "GTCAG": 6801, "GTCTA": 240025, "GTCTT": 162698, "GTCTC": 132260, "GTCTG": 5786, "GTCCA": 139814, "GTCCT": 101875, "GTCCC": 83689, "GTCCG": 51791, "GTCGA": 132908, "GTCGT": 1639656, "GTCGC": 629771, "GTCGG": 25553, "GTGAA": 43868, "GTGAT": 7157, "GTGAC": 667108, "GTGAG": 31783, "GTGTA": 2325392, "GTGTT": 18439, "GTGTC": 119499, "GTGTG": 806120, "GTGCA": 6882, "GTGCT": 92742, "GTGCC": 5330, "GTGCG": 3068, "GTGGA": 55164, "GTGGT": 673415, "GTGGC": 13471, "GTGGG": 87262, "GCAAA": 586392, "GCAAT": 339059, "GCAAC": 199931, "GCAAG": 9378, "GCATA": 324096, "GCATT": 224988, "GCATC": 135248, "GCATG": 3417, "GCACA": 182524, "GCACT": 127398, "GCACC": 82944, "GCACG": 33509, "GCAGA": 18961, "GCAGT": 6344, "GCAGC": 3934, "GCAGG": 9293, "GCTAA": 369875, "GCTAT": 309005, "GCTAC": 163051, "GCTAG": 3695, "GCTTA": 228929, "GCTTT": 206808, "GCTTC": 127318, "GCTTG": 2598, "GCTCA": 162048, "GCTCT": 222318, "GCTCC": 75436, "GCTCG": 36306, "GCTGA": 4022, "GCTGT": 2795, "GCTGC": 2158, "GCTGG": 3072, "GCCAA": 242610, "GCCAT": 210222, "GCCAC": 106737, "GCCAG": 4861, "GCCTA": 146291, "GCCTT": 107415, "GCCTC": 73061, "GCCTG": 2533, "GCCCA": 111766, "GCCCT": 86185, "GCCCC": 89849, "GCCCG": 29961, "GCCGA": 51191, "GCCGT": 605014, "GCCGC": 19163, "GCCGG": 2340, "GCGAA": 104850, "GCGAT": 64398, "GCGAC": 40023, "GCGAG": 9611, "GCGTA": 59488, "GCGTT": 35597, "GCGTC": 1689746, "GCGTG": 9161, "GCGCA": 30924, "GCGCT": 22998, "GCGCC": 23282, "GCGCG": 19190, "GCGGA": 25331, "GCGGT": 2470, "GCGGC": 3050, "GCGGG": 12583, "GGAAA": 8074167, "GGAAT": 2381418, "GGAAC": 1189948, "GGAAG": 8159241, "GGATA": 2207311, "GGATT": 1478132, "GGATC": 1013694, "GGATG": 52868, "GGACA": 1093373, "GGACT": 700160, "GGACC": 479148, "GGACG": 61668, "GGAGA": 10498706, "GGAGT": 505365, "GGAGC": 473783, "GGAGG": 7951828, 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--thread 16 --json output/13-wgbs/fastp/Pum4.fastp.json --html output/13-wgbs/fastp/Pum4.fastp.html " }, "PUM5 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 45570614, "total_bases": 6881162714, "q20_bases": 6796067262, "q30_bases": 6581266633, "q20_rate": 0.987634, "q30_rate": 0.956418, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.211412 }, "after_filtering": { "total_reads": 44823220, "total_bases": 6206927048, "q20_bases": 6151237577, "q30_bases": 5982378322, "q20_rate": 0.991028, "q30_rate": 0.963823, "read1_mean_length": 138, "read2_mean_length": 138, "gc_content": 0.187227 } }, "filtering_result": { "passed_filter_reads": 44823220, "low_quality_reads": 358562, "too_many_N_reads": 930, "too_short_reads": 387902, "too_long_reads": 0 }, "duplication": { "rate": 0.035443 }, "insert_size": { "peak": 230, "unknown": 641886, "histogram": [ 0, 0, 0, 0, 0, 0, 0, 0, 0, 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12564528, "TCATT": 11268472, "TCATC": 5112415, "TCATG": 22487, "TCACA": 7491769, "TCACT": 5600514, "TCACC": 3076175, "TCACG": 233369, "TCAGA": 106435, "TCAGT": 31586, "TCAGC": 8352, "TCAGG": 3715, "TCTAA": 12252541, "TCTAT": 11164567, "TCTAC": 5483962, "TCTAG": 71746, "TCTTA": 8083424, "TCTTT": 8364232, "TCTTC": 4540904, "TCTTG": 15140, "TCTCA": 4907531, "TCTCT": 7056195, "TCTCC": 2488712, "TCTCG": 777703, "TCTGA": 33938, "TCTGT": 27657, "TCTGC": 9445, "TCTGG": 48103, "TCCAA": 7174518, "TCCAT": 5285507, "TCCAC": 2964271, "TCCAG": 30685, "TCCTA": 4521908, "TCCTT": 3668423, "TCCTC": 2334221, "TCCTG": 9369, "TCCCA": 2882788, "TCCCT": 2132880, "TCCCC": 1767978, "TCCCG": 146343, "TCCGA": 480303, "TCCGT": 294382, "TCCGC": 129266, "TCCGG": 2194, "TCGAA": 930447, "TCGAT": 640444, "TCGAC": 322925, "TCGAG": 3841, "TCGTA": 545471, "TCGTT": 354462, "TCGTC": 235240, "TCGTG": 1065263, "TCGCA": 231869, "TCGCT": 212590, "TCGCC": 575882, "TCGCG": 71265, "TCGGA": 1497942, "TCGGT": 498541, "TCGGC": 2661, 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2915, "TGGGG": 89924, "CAAAA": 33198119, "CAAAT": 19495051, "CAAAC": 10932993, "CAAAG": 92761, "CAATA": 20672958, "CAATT": 12773366, "CAATC": 6664211, "CAATG": 38905, "CAACA": 10791565, "CAACT": 6288447, "CAACC": 3955105, "CAACG": 309156, "CAAGA": 109999, "CAAGT": 56988, "CAAGC": 12861, "CAAGG": 7126, "CATAA": 17026345, "CATAT": 15640850, "CATAC": 7799814, "CATAG": 82951, "CATTA": 13439566, "CATTT": 12862367, "CATTC": 4950764, "CATTG": 20993, "CATCA": 7850501, "CATCT": 4817998, "CATCC": 2733422, "CATCG": 258531, "CATGA": 32216, "CATGT": 29515, "CATGC": 12773, "CATGG": 2397, "CACAA": 10802888, "CACAT": 7561965, "CACAC": 6123173, "CACAG": 34686, "CACTA": 7592354, "CACTT": 4909299, "CACTC": 3383090, "CACTG": 13695, "CACCA": 4548526, "CACCT": 3048336, "CACCC": 2136346, "CACCG": 192388, "CACGA": 407745, "CACGT": 259091, "CACGC": 134880, "CACGG": 2040, "CAGAA": 33016, "CAGAT": 252119, "CAGAC": 28229, "CAGAG": 8961, "CAGTA": 16918, "CAGTT": 15183, "CAGTC": 10477, "CAGTG": 23723, "CAGCA": 11890, "CAGCT": 7793, "CAGCC": 9325, "CAGCG": 2254, "CAGGA": 7866, "CAGGT": 2144, "CAGGC": 2096, "CAGGG": 6627, "CTAAA": 17083511, "CTAAT": 10962694, "CTAAC": 6224362, "CTAAG": 72065, "CTATA": 14843603, "CTATT": 9417990, "CTATC": 5463141, "CTATG": 23873, "CTACA": 7506833, "CTACT": 4558667, "CTACC": 2737162, "CTACG": 254007, "CTAGA": 146050, "CTAGT": 12021, "CTAGC": 6123, "CTAGG": 9283, "CTTAA": 10595558, "CTTAT": 8028201, "CTTAC": 4493578, "CTTAG": 54655, "CTTTA": 10204661, "CTTTT": 8702489, "CTTTC": 4792255, "CTTTG": 16946, "CTTCA": 5457963, "CTTCT": 3821440, "CTTCC": 2491104, "CTTCG": 210748, "CTTGA": 18104, "CTTGT": 13312, "CTTGC": 6471, "CTTGG": 2840, "CTCAA": 6448078, "CTCAT": 3993630, "CTCAC": 2813730, "CTCAG": 39365, "CTCTA": 5601612, "CTCTT": 3660293, "CTCTC": 5202869, "CTCTG": 19073, "CTCCA": 2884560, "CTCCT": 2068436, "CTCCC": 1578794, "CTCCG": 149032, "CTCGA": 415284, "CTCGT": 217694, "CTCGC": 148768, "CTCGG": 503914, "CTGAA": 33640, "CTGAT": 20656, "CTGAC": 12087, "CTGAG": 12630, "CTGTA": 22623, "CTGTT": 18224, "CTGTC": 11601, "CTGTG": 7158, "CTGCA": 11273, "CTGCT": 7662, "CTGCC": 6417, "CTGCG": 1641, "CTGGA": 15970, "CTGGT": 1401, "CTGGC": 1216, "CTGGG": 35014, "CCAAA": 11382279, "CCAAT": 6671915, "CCAAC": 3836262, "CCAAG": 36163, "CCATA": 7011777, "CCATT": 5161949, "CCATC": 2779997, "CCATG": 16322, "CCACA": 4118321, "CCACT": 2597467, "CCACC": 2418602, "CCACG": 143802, "CCAGA": 85474, "CCAGT": 12381, "CCAGC": 8000, "CCAGG": 2885, "CCTAA": 6290140, "CCTAT": 5262871, "CCTAC": 2844512, "CCTAG": 38778, "CCTTA": 4549843, "CCTTT": 4333755, "CCTTC": 2121793, "CCTTG": 9456, "CCTCA": 2555429, "CCTCT": 2397005, "CCTCC": 1597521, "CCTCG": 151511, "CCTGA": 13540, "CCTGT": 10506, "CCTGC": 6542, "CCTGG": 2203, "CCCAA": 4465792, "CCCAT": 2697269, "CCCAC": 2115570, "CCCAG": 41668, "CCCTA": 3201623, "CCCTT": 2181678, "CCCTC": 1584218, "CCCTG": 9394, "CCCCA": 2491246, "CCCCT": 1895515, "CCCCC": 4283137, "CCCCG": 141809, "CCCGA": 279149, "CCCGT": 136976, "CCCGC": 106457, "CCCGG": 5737, "CCGAA": 624802, "CCGAT": 455484, "CCGAC": 255560, "CCGAG": 5291, "CCGTA": 760963, "CCGTT": 220400, "CCGTC": 186255, "CCGTG": 6911, "CCGCA": 129058, "CCGCT": 127350, "CCGCC": 117545, "CCGCG": 50482, "CCGGA": 5046, "CCGGT": 3892, "CCGGC": 1641, "CCGGG": 3205, "CGAAA": 1466217, "CGAAT": 814045, "CGAAC": 521154, "CGAAG": 17427, "CGATA": 875279, "CGATT": 545413, "CGATC": 452481, "CGATG": 3152, "CGACA": 391714, "CGACT": 285066, "CGACC": 229836, "CGACG": 130737, "CGAGA": 11055, "CGAGT": 4282, "CGAGC": 3598, "CGAGG": 6079, "CGTAA": 762701, "CGTAT": 1000641, "CGTAC": 333691, "CGTAG": 9180, "CGTTA": 466205, "CGTTT": 406757, "CGTTC": 183544, "CGTTG": 6517, "CGTCA": 317450, "CGTCT": 239942, "CGTCC": 147300, "CGTCG": 1208717, "CGTGA": 5736, "CGTGT": 1049385, "CGTGC": 3738, "CGTGG": 27440, "CGCAA": 323852, "CGCAT": 217784, "CGCAC": 137075, "CGCAG": 5257, "CGCTA": 271983, "CGCTT": 199771, "CGCTC": 127149, "CGCTG": 1349, "CGCCA": 182842, "CGCCT": 107473, "CGCCC": 103189, "CGCCG": 472953, "CGCGA": 114698, "CGCGT": 67275, "CGCGC": 51430, "CGCGG": 2111, "CGGAA": 1499316, "CGGAT": 3313, "CGGAC": 3295, "CGGAG": 12111, "CGGTA": 3744, "CGGTT": 4523, "CGGTC": 1859, "CGGTG": 490383, "CGGCA": 2896, "CGGCT": 1430, "CGGCC": 1978, "CGGCG": 2211, "CGGGA": 18149, "CGGGT": 2403, "CGGGC": 1707, "CGGGG": 11017, "GAAAA": 4859924, "GAAAT": 2034170, "GAAAC": 1071824, "GAAAG": 3060935, "GAATA": 1916471, "GAATT": 1293726, "GAATC": 785511, "GAATG": 28640, "GAACA": 991473, "GAACT": 597117, "GAACC": 383398, "GAACG": 110632, "GAAGA": 5726167, "GAAGT": 197009, "GAAGC": 183476, "GAAGG": 3066549, "GATAA": 1766823, "GATAT": 1236419, "GATAC": 705891, "GATAG": 38492, "GATTA": 1126483, "GATTT": 923647, "GATTC": 477982, "GATTG": 6879, "GATCA": 778170, "GATCT": 1029265, "GATCC": 278819, "GATCG": 1652388, "GATGA": 33758, "GATGT": 7002, "GATGC": 3481, "GATGG": 35328, "GACAA": 1551413, "GACAT": 547914, "GACAC": 313889, "GACAG": 8534, "GACTA": 603722, "GACTT": 380703, "GACTC": 221927, "GACTG": 2434, "GACCA": 380813, "GACCT": 219337, "GACCC": 166007, "GACCG": 54150, "GACGA": 103670, "GACGT": 91470, "GACGC": 38563, "GACGG": 5922, "GAGAA": 5394981, "GAGAT": 1707321, "GAGAC": 773630, "GAGAG": 5623678, "GAGTA": 293671, "GAGTT": 190021, "GAGTC": 131361, "GAGTG": 748884, "GAGCA": 256451, "GAGCT": 194639, "GAGCC": 116638, "GAGCG": 1237875, "GAGGA": 6713743, "GAGGT": 337540, "GAGGC": 319042, "GAGGG": 4179284, "GTAAA": 683899, "GTAAT": 394628, "GTAAC": 247208, "GTAAG": 20268, "GTATA": 447731, "GTATT": 331821, "GTATC": 596460, "GTATG": 5223, "GTACA": 272557, "GTACT": 152660, "GTACC": 103219, "GTACG": 50766, "GTAGA": 525361, "GTAGT": 6387, "GTAGC": 2825, "GTAGG": 931144, "GTTAA": 355038, "GTTAT": 292504, "GTTAC": 165765, "GTTAG": 5957, "GTTTA": 292481, "GTTTT": 261845, "GTTTC": 148183, "GTTTG": 8634, "GTTCA": 168277, "GTTCT": 109885, "GTTCC": 76199, "GTTCG": 23159, "GTTGA": 4650, "GTTGT": 14146, "GTTGC": 20042, "GTTGG": 7921, "GTCAA": 258200, "GTCAT": 217059, "GTCAC": 134584, "GTCAG": 4485, "GTCTA": 175793, "GTCTT": 119336, "GTCTC": 97127, "GTCTG": 3852, "GTCCA": 98912, "GTCCT": 72721, "GTCCC": 60557, "GTCCG": 37473, "GTCGA": 93487, "GTCGT": 1138212, "GTCGC": 466721, "GTCGG": 18128, "GTGAA": 37403, "GTGAT": 4720, "GTGAC": 665469, "GTGAG": 23405, "GTGTA": 1468173, "GTGTT": 14665, "GTGTC": 68626, "GTGTG": 779910, "GTGCA": 4663, "GTGCT": 68402, "GTGCC": 3860, "GTGCG": 2228, "GTGGA": 40489, "GTGGT": 498004, "GTGGC": 11493, "GTGGG": 63714, "GCAAA": 437970, "GCAAT": 253757, "GCAAC": 147989, "GCAAG": 6552, "GCATA": 245024, "GCATT": 169962, "GCATC": 101773, "GCATG": 2119, "GCACA": 135034, "GCACT": 94193, "GCACC": 60838, "GCACG": 24454, "GCAGA": 13390, "GCAGT": 4356, "GCAGC": 2571, "GCAGG": 6357, "GCTAA": 276697, "GCTAT": 228748, "GCTAC": 122154, "GCTAG": 2521, "GCTTA": 175288, "GCTTT": 155249, "GCTTC": 97582, "GCTTG": 1512, "GCTCA": 118031, "GCTCT": 163544, "GCTCC": 55655, "GCTCG": 26444, "GCTGA": 2360, "GCTGT": 1584, "GCTGC": 1259, "GCTGG": 1606, "GCCAA": 170741, "GCCAT": 149161, "GCCAC": 75561, "GCCAG": 3049, "GCCTA": 106474, "GCCTT": 77423, "GCCTC": 52386, "GCCTG": 1330, "GCCCA": 78082, "GCCCT": 60370, "GCCCC": 63906, "GCCCG": 21605, "GCCGA": 36317, "GCCGT": 449542, "GCCGC": 13797, "GCCGG": 1638, "GCGAA": 77310, "GCGAT": 47859, "GCGAC": 29541, "GCGAG": 7199, "GCGTA": 44146, "GCGTT": 26552, "GCGTC": 1167544, "GCGTG": 6648, "GCGCA": 23117, "GCGCT": 16756, "GCGCC": 16983, "GCGCG": 14121, "GCGGA": 18663, "GCGGT": 1735, "GCGGC": 2135, "GCGGG": 9308, "GGAAA": 5994768, "GGAAT": 1789106, "GGAAC": 873353, "GGAAG": 5974282, "GGATA": 1661302, "GGATT": 1116236, "GGATC": 716119, "GGATG": 39942, "GGACA": 806119, "GGACT": 527145, "GGACC": 330367, "GGACG": 45237, "GGAGA": 7862106, "GGAGT": 371794, "GGAGC": 351763, "GGAGG": 5966115, "GGTAA": 292764, "GGTAT": 174139, "GGTAC": 120032, "GGTAG": 23927, "GGTTA": 172919, "GGTTT": 128955, "GGTTC": 77294, "GGTTG": 27644, "GGTCA": 140523, "GGTCT": 71057, "GGTCC": 50019, "GGTCG": 466657, "GGTGA": 23584, "GGTGT": 22161, "GGTGC": 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200228, "GGGGT": 18582, "GGGGC": 10146, "GGGGG": 731697 }, "overrepresented_sequences": {} }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/PUM5 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/PUM5 (paired)_R2.fastq.gz -o output/13-wgbs/trimmed/Pum5_R1.fq.gz -O output/13-wgbs/trimmed/Pum5_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs/fastp/Pum5.fastp.json --html output/13-wgbs/fastp/Pum5.fastp.html " }, "Qui1 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 78650066, "total_bases": 11876159966, "q20_bases": 11734121119, "q30_bases": 11383518712, "q20_rate": 0.98804, "q30_rate": 0.958518, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.21444 }, "after_filtering": { "total_reads": 77441228, "total_bases": 10679350373, "q20_bases": 10588814231, "q30_bases": 10318661776, "q20_rate": 0.991522, "q30_rate": 0.966226, "read1_mean_length": 137, "read2_mean_length": 137, "gc_content": 0.189486 } }, "filtering_result": { "passed_filter_reads": 77441228, "low_quality_reads": 615766, "too_many_N_reads": 1620, "too_short_reads": 591452, "too_long_reads": 0 }, "duplication": { "rate": 0.0590184 }, "insert_size": { "peak": 182, "unknown": 761249, "histogram": [ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 2760, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 340, 328, 329, 340, 371, 353, 327, 333, 334, 330, 313, 345, 346, 388, 379, 385, 418, 401, 400, 404, 415, 416, 437, 427, 453, 513, 453, 515, 520, 559, 534, 566, 587, 666, 642, 633, 725, 679, 704, 775, 784, 815, 842, 915, 908, 963, 1019, 1065, 1082, 1128, 1209, 1247, 1323, 1393, 1448, 1555, 1631, 1722, 1876, 1946, 1977, 2047, 2186, 2272, 2430, 2578, 2763, 2786, 2873, 3117, 3243, 3380, 3539, 3730, 3826, 3946, 4198, 4326, 4626, 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895969, "TTAGA": 587203, "TTAGT": 51306, "TTAGC": 25122, "TTAGG": 17528, "TTTAA": 49584886, "TTTAT": 37612043, "TTTAC": 19289626, "TTTAG": 275811, "TTTTA": 41208928, "TTTTT": 36838886, "TTTTC": 19352443, "TTTTG": 103069, "TTTCA": 23666859, "TTTCT": 16266627, "TTTCC": 8845509, "TTTCG": 749015, "TTTGA": 117657, "TTTGT": 78897, "TTTGC": 34696, "TTTGG": 21954, "TTCAA": 26727300, "TTCAT": 18989034, "TTCAC": 9753247, "TTCAG": 348807, "TTCTA": 16957479, "TTCTT": 13685329, "TTCTC": 7409922, "TTCTG": 48644, "TTCCA": 9774582, "TTCCT": 7074515, "TTCCC": 4185126, "TTCCG": 589577, "TTCGA": 970752, "TTCGT": 619784, "TTCGC": 319010, "TTCGG": 8926, "TTGAA": 143153, "TTGAT": 97001, "TTGAC": 50005, "TTGAG": 14246, "TTGTA": 77311, "TTGTT": 76894, "TTGTC": 44661, "TTGTG": 19277, "TTGCA": 41460, "TTGCT": 30290, "TTGCC": 45730, "TTGCG": 4579, "TTGGA": 19014, "TTGGT": 12693, "TTGGC": 5835, "TTGGG": 25383, "TCAAA": 37542837, "TCAAT": 23250774, "TCAAC": 12716346, "TCAAG": 109543, "TCATA": 21620413, "TCATT": 18649070, "TCATC": 8800572, "TCATG": 53741, "TCACA": 13143175, "TCACT": 9330552, "TCACC": 5487268, "TCACG": 408068, "TCAGA": 264145, "TCAGT": 277848, "TCAGC": 19813, "TCAGG": 11343, "TCTAA": 21084701, "TCTAT": 18002158, "TCTAC": 9316727, "TCTAG": 157012, "TCTTA": 13493865, "TCTTT": 13374979, "TCTTC": 7664645, "TCTTG": 36388, "TCTCA": 8336507, "TCTCT": 10641469, "TCTCC": 4161331, "TCTCG": 1367815, "TCTGA": 78727, "TCTGT": 62570, "TCTGC": 23104, "TCTGG": 87027, "TCCAA": 12670020, "TCCAT": 9066586, "TCCAC": 5210734, "TCCAG": 71341, "TCCTA": 7790384, "TCCTT": 6055958, "TCCTC": 3982559, "TCCTG": 24435, "TCCCA": 5122003, "TCCCT": 3588737, "TCCCC": 3094057, "TCCCG": 262581, "TCCGA": 851943, "TCCGT": 519914, "TCCGC": 225862, "TCCGG": 4466, "TCGAA": 1667251, "TCGAT": 1126954, "TCGAC": 580178, "TCGAG": 8149, "TCGTA": 935178, "TCGTT": 596795, "TCGTC": 405159, "TCGTG": 2342787, "TCGCA": 401443, "TCGCT": 367293, "TCGCC": 1006461, "TCGCG": 127843, "TCGGA": 3518050, "TCGGT": 882281, "TCGGC": 5075, 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"CTGAT": 55291, "CTGAC": 31026, "CTGAG": 26839, "CTGTA": 57022, "CTGTT": 43001, "CTGTC": 25586, "CTGTG": 18357, "CTGCA": 29394, "CTGCT": 20710, "CTGCC": 15000, "CTGCG": 3514, "CTGGA": 32322, "CTGGT": 5311, "CTGGC": 4968, "CTGGG": 65669, "CCAAA": 20737161, "CCAAT": 11884636, "CCAAC": 7017027, "CCAAG": 87311, "CCATA": 12360971, "CCATT": 8917291, "CCATC": 4968880, "CCATG": 40596, "CCACA": 7525833, "CCACT": 4611527, "CCACC": 4466381, "CCACG": 264740, "CCAGA": 197615, "CCAGT": 22229, "CCAGC": 18536, "CCAGG": 8851, "CCTAA": 11279017, "CCTAT": 9070482, "CCTAC": 5048398, "CCTAG": 90905, "CCTTA": 7717921, "CCTTT": 7188014, "CCTTC": 3694585, "CCTTG": 23075, "CCTCA": 4580498, "CCTCT": 4083576, "CCTCC": 2798704, "CCTCG": 269749, "CCTGA": 36046, "CCTGT": 25903, "CCTGC": 15699, "CCTGG": 7875, "CCCAA": 8199268, "CCCAT": 4764519, "CCCAC": 3848455, "CCCAG": 86442, "CCCTA": 5831667, "CCCTT": 3776971, "CCCTC": 2758453, "CCCTG": 22458, "CCCCA": 4531211, "CCCCT": 3361589, "CCCCC": 8009339, "CCCCG": 264079, "CCCGA": 521625, "CCCGT": 245915, "CCCGC": 187518, "CCCGG": 9480, "CCGAA": 1167524, "CCGAT": 804082, "CCGAC": 451956, "CCGAG": 9788, "CCGTA": 1320767, "CCGTT": 377430, "CCGTC": 334931, "CCGTG": 15990, "CCGCA": 229439, "CCGCT": 214060, "CCGCC": 211369, "CCGCG": 92528, "CCGGA": 10942, "CCGGT": 6605, "CCGGC": 3327, "CCGGG": 5404, "CGAAA": 2669279, "CGAAT": 1450873, "CGAAC": 959263, "CGAAG": 35401, "CGATA": 1531955, "CGATT": 932138, "CGATC": 823534, "CGATG": 6888, "CGACA": 716636, "CGACT": 492343, "CGACC": 414183, "CGACG": 242319, "CGAGA": 21875, "CGAGT": 7014, "CGAGC": 6736, "CGAGG": 10512, "CGTAA": 1331323, "CGTAT": 1703698, "CGTAC": 585609, "CGTAG": 19512, "CGTTA": 788325, "CGTTT": 673524, "CGTTC": 317507, "CGTTG": 14185, "CGTCA": 530588, "CGTCT": 424112, "CGTCC": 263313, "CGTCG": 2657056, "CGTGA": 10844, "CGTGT": 2275704, "CGTGC": 7023, "CGTGG": 65876, "CGCAA": 575213, "CGCAT": 372647, "CGCAC": 243011, "CGCAG": 9947, "CGCTA": 481231, "CGCTT": 326633, "CGCTC": 228881, "CGCTG": 3303, "CGCCA": 333622, "CGCCT": 186687, "CGCCC": 191988, "CGCCG": 819784, "CGCGA": 208347, "CGCGT": 116850, "CGCGC": 93386, "CGCGG": 3780, "CGGAA": 3479100, "CGGAT": 6831, "CGGAC": 6197, "CGGAG": 22482, "CGGTA": 7304, "CGGTT": 9112, "CGGTC": 4063, "CGGTG": 863117, "CGGCA": 5508, "CGGCT": 3045, "CGGCC": 4121, "CGGCG": 4068, "CGGGA": 35701, "CGGGT": 4143, "CGGGC": 3438, "CGGGG": 19310, "GAAAA": 8264382, "GAAAT": 3473570, "GAAAC": 1934144, "GAAAG": 5400793, "GAATA": 3144566, "GAATT": 2111414, "GAATC": 1510113, "GAATG": 54824, "GAACA": 1786670, "GAACT": 1034766, "GAACC": 787191, "GAACG": 208858, "GAAGA": 10503308, "GAAGT": 352779, "GAAGC": 319424, "GAAGG": 5037599, "GATAA": 3035839, "GATAT": 1988705, "GATAC": 1240678, "GATAG": 62173, "GATTA": 1943608, "GATTT": 1403256, "GATTC": 849761, "GATTG": 17587, "GATCA": 1630248, "GATCT": 1855919, "GATCC": 591460, "GATCG": 3899406, "GATGA": 56554, "GATGT": 15755, "GATGC": 8428, "GATGG": 55595, "GACAA": 1675503, "GACAT": 961828, "GACAC": 584980, "GACAG": 18950, "GACTA": 1014183, "GACTT": 609328, "GACTC": 400576, "GACTG": 7609, "GACCA": 817989, "GACCT": 448061, "GACCC": 354624, "GACCG": 110456, "GACGA": 192009, "GACGT": 164823, "GACGC": 73505, "GACGG": 10440, "GAGAA": 8981668, "GAGAT": 2910253, "GAGAC": 1410583, "GAGAG": 9278114, "GAGTA": 491840, "GAGTT": 326772, "GAGTC": 292211, "GAGTG": 1486994, "GAGCA": 409943, "GAGCT": 315624, "GAGCC": 266332, "GAGCG": 2806875, "GAGGA": 11200450, "GAGGT": 602552, "GAGGC": 552432, "GAGGG": 6911551, "GTAAA": 1205055, "GTAAT": 1913869, "GTAAC": 492274, "GTAAG": 41303, "GTATA": 762736, "GTATT": 547412, "GTATC": 1009997, "GTATG": 10796, "GTACA": 489955, "GTACT": 278412, "GTACC": 189551, "GTACG": 92326, "GTAGA": 934444, "GTAGT": 14298, "GTAGC": 6257, "GTAGG": 1972462, "GTTAA": 640074, "GTTAT": 519434, "GTTAC": 276628, "GTTAG": 14682, "GTTTA": 483969, "GTTTT": 430358, "GTTTC": 248741, "GTTTG": 14297, "GTTCA": 305957, "GTTCT": 200258, "GTTCC": 134709, "GTTCG": 40954, "GTTGA": 10149, "GTTGT": 30118, "GTTGC": 34205, "GTTGG": 16254, "GTCAA": 528882, "GTCAT": 484763, "GTCAC": 270028, "GTCAG": 11849, "GTCTA": 322086, "GTCTT": 214935, "GTCTC": 176472, "GTCTG": 9483, "GTCCA": 202434, "GTCCT": 145802, "GTCCC": 116897, "GTCCG": 71403, "GTCGA": 197644, "GTCGT": 2506369, "GTCGC": 815327, "GTCGG": 37703, "GTGAA": 32417, "GTGAT": 12286, "GTGAC": 8009, "GTGAG": 41604, "GTGTA": 4286039, "GTGTT": 19141, "GTGTC": 254310, "GTGTG": 131578, "GTGCA": 11117, "GTGCT": 121883, "GTGCC": 7361, "GTGCG": 4168, "GTGGA": 73512, "GTGGT": 876270, "GTGGC": 9602, "GTGGG": 118281, "GCAAA": 743070, "GCAAT": 429302, "GCAAC": 257197, "GCAAG": 14303, "GCATA": 397301, "GCATT": 275358, "GCATC": 166635, "GCATG": 6709, "GCACA": 237349, "GCACT": 165728, "GCACC": 111266, "GCACG": 45124, "GCAGA": 27974, "GCAGT": 9837, "GCAGC": 6864, "GCAGG": 14515, "GCTAA": 467725, "GCTAT": 402607, "GCTAC": 204229, "GCTAG": 6171, "GCTTA": 269366, "GCTTT": 256678, "GCTTC": 149655, "GCTTG": 5349, "GCTCA": 218981, "GCTCT": 294876, "GCTCC": 100352, "GCTCG": 49144, "GCTGA": 8187, "GCTGT": 5881, "GCTGC": 4651, "GCTGG": 7148, "GCCAA": 356491, "GCCAT": 305499, "GCCAC": 156957, "GCCAG": 8837, "GCCTA": 199456, "GCCTT": 150721, "GCCTC": 102811, "GCCTG": 6066, "GCCCA": 167848, "GCCCT": 131039, "GCCCC": 128612, "GCCCG": 41430, "GCCGA": 74157, "GCCGT": 775731, "GCCGC": 27026, "GCCGG": 3418, "GCGAA": 137165, "GCGAT": 83699, "GCGAC": 52989, "GCGAG": 11880, "GCGTA": 77086, "GCGTT": 45266, "GCGTC": 2612346, "GCGTG": 12658, "GCGCA": 38955, "GCGCT": 30788, "GCGCC": 32055, "GCGCG": 25414, "GCGGA": 32901, "GCGGT": 3632, "GCGGC": 4350, "GCGGG": 16114, "GGAAA": 10399479, "GGAAT": 2953639, "GGAAC": 1581252, "GGAAG": 10925049, "GGATA": 2730290, "GGATT": 1808919, "GGATC": 1486824, "GGATG": 64152, "GGACA": 1436979, "GGACT": 865207, "GGACC": 742361, "GGACG": 82303, "GGAGA": 13188017, "GGAGT": 668460, "GGAGC": 610536, "GGAGG": 9933251, "GGTAA": 514723, "GGTAT": 259403, "GGTAC": 217877, "GGTAG": 39440, "GGTTA": 315242, "GGTTT": 190847, "GGTTC": 146376, 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27237, "TTGCC": 23595, "TTGCG": 3033, "TTGGA": 7723, "TTGGT": 6220, "TTGGC": 3949, "TTGGG": 8879, "TCAAA": 36403785, "TCAAT": 22618563, "TCAAC": 12324662, "TCAAG": 35705, "TCATA": 21014033, "TCATT": 17876727, "TCATC": 8532989, "TCATG": 48043, "TCACA": 12732392, "TCACT": 9009935, "TCACC": 5247625, "TCACG": 394232, "TCAGA": 38229, "TCAGT": 130988, "TCAGC": 14360, "TCAGG": 6261, "TCTAA": 20587603, "TCTAT": 17592170, "TCTAC": 9081428, "TCTAG": 39874, "TCTTA": 13154169, "TCTTT": 13047745, "TCTTC": 7347085, "TCTTG": 30506, "TCTCA": 8059544, "TCTCT": 10266048, "TCTCC": 3939555, "TCTCG": 834059, "TCTGA": 71164, "TCTGT": 45822, "TCTGC": 20070, "TCTGG": 80898, "TCCAA": 12256753, "TCCAT": 8812559, "TCCAC": 4993346, "TCCAG": 21569, "TCCTA": 7544971, "TCCTT": 5816821, "TCCTC": 3770387, "TCCTG": 21382, "TCCCA": 4882775, "TCCCT": 3396045, "TCCCC": 2715809, "TCCCG": 246733, "TCCGA": 792393, "TCCGT": 491979, "TCCGC": 213988, "TCCGG": 2201, "TCGAA": 1598006, "TCGAT": 1032422, "TCGAC": 555472, "TCGAG": 3472, "TCGTA": 899526, "TCGTT": 572419, "TCGTC": 385408, "TCGTG": 385700, "TCGCA": 383735, "TCGCT": 353036, "TCGCC": 593722, "TCGCG": 122791, "TCGGA": 28669, "TCGGT": 376824, "TCGGC": 2201, "TCGGG": 7949, "TGAAA": 234151, "TGAAT": 127930, "TGAAC": 68652, "TGAAG": 16435, "TGATA": 144700, "TGATT": 95716, "TGATC": 49183, "TGATG": 7006, "TGACA": 78501, "TGACT": 42456, "TGACC": 37758, "TGACG": 5187, "TGAGA": 21686, "TGAGT": 7008, "TGAGC": 4818, "TGAGG": 24610, "TGTAA": 446825, "TGTAT": 101462, "TGTAC": 43405, "TGTAG": 707156, "TGTTA": 70384, "TGTTT": 86667, "TGTTC": 41324, "TGTTG": 7921, "TGTCA": 90915, "TGTCT": 35235, "TGTCC": 23591, "TGTCG": 56624, "TGTGA": 7765, "TGTGT": 10779, "TGTGC": 9359, "TGTGG": 11500, "TGCAA": 55271, "TGCAT": 46045, "TGCAC": 24838, "TGCAG": 6160, "TGCTA": 34212, "TGCTT": 30221, "TGCTC": 32878, "TGCTG": 5788, "TGCCA": 22442, "TGCCT": 16348, "TGCCC": 12757, "TGCCG": 10159, "TGCGA": 3622, "TGCGT": 3095, "TGCGC": 3057, "TGCGG": 1544, "TGGAA": 26708, "TGGAT": 8011, 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7673, "GTTCA": 195624, "GTTCT": 134997, "GTTCC": 93665, "GTTCG": 34437, "GTTGA": 5900, "GTTGT": 9371, "GTTGC": 12256, "GTTGG": 5447, "GTCAA": 304541, "GTCAT": 296004, "GTCAC": 166294, "GTCAG": 4704, "GTCTA": 238285, "GTCTT": 152649, "GTCTC": 115309, "GTCTG": 4811, "GTCCA": 118517, "GTCCT": 89337, "GTCCC": 76056, "GTCCG": 63732, "GTCGA": 116693, "GTCGT": 451524, "GTCGC": 395314, "GTCGG": 8316, "GTGAA": 12086, "GTGAT": 7312, "GTGAC": 4480, "GTGAG": 10540, "GTGTA": 1032919, "GTGTT": 9112, "GTGTC": 95720, "GTGTG": 12145, "GTGCA": 4877, "GTGCT": 13504, "GTGCC": 3444, "GTGCG": 1511, "GTGGA": 11510, "GTGGT": 386813, "GTGGC": 4051, "GTGGG": 16484, "GCAAA": 403715, "GCAAT": 247139, "GCAAC": 146807, "GCAAG": 5559, "GCATA": 245408, "GCATT": 175021, "GCATC": 100292, "GCATG": 4734, "GCACA": 138415, "GCACT": 96701, "GCACC": 61596, "GCACG": 37755, "GCAGA": 7235, "GCAGT": 5405, "GCAGC": 4087, "GCAGG": 5768, "GCTAA": 281490, "GCTAT": 239114, "GCTAC": 122055, "GCTAG": 2639, "GCTTA": 169427, "GCTTT": 166994, "GCTTC": 94822, "GCTTG": 3801, "GCTCA": 124861, "GCTCT": 109968, "GCTCC": 53809, "GCTCG": 35187, "GCTGA": 5586, "GCTGT": 4371, "GCTGC": 3633, "GCTGG": 5394, "GCCAA": 176716, "GCCAT": 167646, "GCCAC": 88137, "GCCAG": 4611, "GCCTA": 114907, "GCCTT": 81541, "GCCTC": 56578, "GCCTG": 5088, "GCCCA": 89591, "GCCCT": 65989, "GCCCC": 69238, "GCCCG": 32530, "GCCGA": 56167, "GCCGT": 389767, "GCCGC": 19909, "GCCGG": 1522, "GCGAA": 102598, "GCGAT": 70430, "GCGAC": 41914, "GCGAG": 2339, "GCGTA": 58767, "GCGTT": 35705, "GCGTC": 404489, "GCGTG": 2486, "GCGCA": 30138, "GCGCT": 25506, "GCGCC": 25380, "GCGCG": 17478, "GCGGA": 2753, "GCGGT": 1423, "GCGGC": 1593, "GCGGG": 3103, "GGAAA": 953929, "GGAAT": 314184, "GGAAC": 150267, "GGAAG": 353657, "GGATA": 319107, "GGATT": 232795, "GGATC": 157194, "GGATG": 17873, "GGACA": 143627, "GGACT": 91833, "GGACC": 78279, "GGACG": 7999, "GGAGA": 494540, "GGAGT": 47550, "GGAGC": 37732, "GGAGG": 306442, "GGTAA": 60900, "GGTAT": 36878, "GGTAC": 27152, "GGTAG": 9243, "GGTTA": 44278, "GGTTT": 33132, "GGTTC": 21315, "GGTTG": 15027, "GGTCA": 39729, "GGTCT": 18651, "GGTCC": 16060, "GGTCG": 373056, "GGTGA": 10007, "GGTGT": 7226, "GGTGC": 3439, "GGTGG": 388594, "GGCAA": 48512, "GGCAT": 28532, "GGCAC": 19336, "GGCAG": 6146, "GGCTA": 40003, "GGCTT": 23631, "GGCTC": 18253, "GGCTG": 5293, "GGCCA": 32492, "GGCCT": 17786, "GGCCC": 17909, "GGCCG": 3609, "GGCGA": 4321, "GGCGT": 2863, "GGCGC": 2458, "GGCGG": 3358, "GGGAA": 744276, "GGGAT": 181084, "GGGAC": 76338, "GGGAG": 293667, "GGGTA": 35612, "GGGTT": 27336, "GGGTC": 18946, "GGGTG": 15172, "GGGCA": 25590, "GGGCT": 21280, "GGGCC": 17325, "GGGCG": 4166, "GGGGA": 313830, "GGGGT": 31676, "GGGGC": 18071, "GGGGG": 1307589 }, "overrepresented_sequences": {} }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/Qui1 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/Qui1 (paired)_R2.fastq.gz -o output/13-wgbs/trimmed/Qui1_R1.fq.gz -O output/13-wgbs/trimmed/Qui1_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs/fastp/Qui1.fastp.json --html output/13-wgbs/fastp/Qui1.fastp.html " }, "Qui2 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 74497804, "total_bases": 11249168404, "q20_bases": 11099839912, "q30_bases": 10711151271, "q20_rate": 0.986725, "q30_rate": 0.952173, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.213081 }, "after_filtering": { "total_reads": 73255454, "total_bases": 10133522387, "q20_bases": 10035578092, "q30_bases": 9728707541, "q20_rate": 0.990335, "q30_rate": 0.960052, "read1_mean_length": 138, "read2_mean_length": 138, "gc_content": 0.187922 } }, "filtering_result": { "passed_filter_reads": 73255454, "low_quality_reads": 644014, "too_many_N_reads": 1892, "too_short_reads": 596444, "too_long_reads": 0 }, "duplication": { "rate": 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14234, "TGGCC": 8362, "TGGCG": 7010, "TGGGA": 104217, "TGGGT": 17011, "TGGGC": 9644, "TGGGG": 170528, "CAAAA": 52866693, "CAAAT": 31274376, "CAAAC": 17325688, "CAAAG": 184362, "CAATA": 31894170, "CAATT": 21128157, "CAATC": 10643093, "CAATG": 84813, "CAACA": 17050718, "CAACT": 10135815, "CAACC": 6299192, "CAACG": 546204, "CAAGA": 220234, "CAAGT": 45916, "CAAGC": 27510, "CAAGG": 16490, "CATAA": 27194902, "CATAT": 25403239, "CATAC": 12295047, "CATAG": 156417, "CATTA": 21698755, "CATTT": 21867694, "CATTC": 8180011, "CATTG": 52610, "CATCA": 12546580, "CATCT": 7923066, "CATCC": 4457331, "CATCG": 471331, "CATGA": 80122, "CATGT": 68770, "CATGC": 31546, "CATGG": 8779, "CACAA": 17025943, "CACAT": 12004831, "CACAC": 9326138, "CACAG": 71460, "CACTA": 11882480, "CACTT": 8087383, "CACTC": 5243791, "CACTG": 36761, "CACCA": 7241573, "CACCT": 4953056, "CACCC": 3407219, "CACCG": 341075, "CACGA": 717643, "CACGT": 467608, "CACGC": 241133, "CACGG": 5578, "CAGAA": 77315, "CAGAT": 485562, "CAGAC": 61598, 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"CCCCA": 4056306, "CCCCT": 3110390, "CCCCC": 6650651, "CCCCG": 259988, "CCCGA": 504737, "CCCGT": 247263, "CCCGC": 198576, "CCCGG": 15681, "CCGAA": 1135380, "CCGAT": 829147, "CCGAC": 470838, "CCGAG": 13123, "CCGTA": 1291917, "CCGTT": 412797, "CCGTC": 346031, "CCGTG": 19899, "CCGCA": 238651, "CCGCT": 244872, "CCGCC": 217964, "CCGCG": 95155, "CCGGA": 11749, "CCGGT": 8778, "CCGGC": 11519, "CCGGG": 8065, "CGAAA": 2626105, "CGAAT": 1478870, "CGAAC": 919320, "CGAAG": 34948, "CGATA": 1545539, "CGATT": 1035447, "CGATC": 805337, "CGATG": 8581, "CGACA": 701053, "CGACT": 521343, "CGACC": 419965, "CGACG": 238006, "CGAGA": 23892, "CGAGT": 10687, "CGAGC": 8362, "CGAGG": 13534, "CGTAA": 1360673, "CGTAT": 1748093, "CGTAC": 603479, "CGTAG": 18091, "CGTTA": 854074, "CGTTT": 786436, "CGTTC": 344886, "CGTTG": 13119, "CGTCA": 569167, "CGTCT": 447239, "CGTCC": 272941, "CGTCG": 2115480, "CGTGA": 11337, "CGTGT": 1832880, "CGTGC": 8750, "CGTGG": 51669, "CGCAA": 581962, "CGCAT": 395624, "CGCAC": 244590, "CGCAG": 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"GATGG": 74719, "GACAA": 1529663, "GACAT": 939124, "GACAC": 533275, "GACAG": 21442, "GACTA": 980191, "GACTT": 653876, "GACTC": 393649, "GACTG": 9821, "GACCA": 760162, "GACCT": 440668, "GACCC": 343744, "GACCG": 113115, "GACGA": 192071, "GACGT": 178847, "GACGC": 71859, "GACGG": 12854, "GAGAA": 8636735, "GAGAT": 2842206, "GAGAC": 1328608, "GAGAG": 9199522, "GAGTA": 459243, "GAGTT": 338525, "GAGTC": 281291, "GAGTG": 1273776, "GAGCA": 372075, "GAGCT": 307588, "GAGCC": 247622, "GAGCG": 2172732, "GAGGA": 10979974, "GAGGT": 587951, "GAGGC": 515114, "GAGGG": 6976606, "GTAAA": 1175264, "GTAAT": 698672, "GTAAC": 423211, "GTAAG": 41935, "GTATA": 784651, "GTATT": 604893, "GTATC": 989213, "GTATG": 12019, "GTACA": 476643, "GTACT": 280409, "GTACC": 183477, "GTACG": 96692, "GTAGA": 894680, "GTAGT": 15746, "GTAGC": 7303, "GTAGG": 1600561, "GTTAA": 652459, "GTTAT": 1473627, "GTTAC": 288966, "GTTAG": 13651, "GTTTA": 536496, "GTTTT": 525656, "GTTTC": 285077, "GTTTG": 17256, "GTTCA": 324219, "GTTCT": 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641972, "GAACT": 418831, "GAACC": 312633, "GAACG": 133927, "GAAGA": 704002, "GAAGT": 61402, "GAAGC": 47713, "GAAGG": 369037, "GATAA": 1243647, "GATAT": 968933, "GATAC": 499922, "GATAG": 28629, "GATTA": 811703, "GATTT": 779169, "GATTC": 367840, "GATTG": 6734, "GATCA": 518159, "GATCT": 824752, "GATCC": 225524, "GATCG": 122042, "GATGA": 30559, "GATGT": 9611, "GATGC": 5535, "GATGG": 25914, "GACAA": 613099, "GACAT": 401869, "GACAC": 229535, "GACAG": 7961, "GACTA": 413903, "GACTT": 307171, "GACTC": 153820, "GACTG": 5393, "GACCA": 296973, "GACCT": 181726, "GACCC": 158329, "GACCG": 71614, "GACGA": 124866, "GACGT": 87795, "GACGC": 49433, "GACGG": 3251, "GAGAA": 793277, "GAGAT": 389338, "GAGAC": 169954, "GAGAG": 543344, "GAGTA": 74990, "GAGTT": 60256, "GAGTC": 40603, "GAGTG": 381327, "GAGCA": 56486, "GAGCT": 46176, "GAGCC": 37680, "GAGCG": 122923, "GAGGA": 628605, "GAGGT": 55112, "GAGGC": 43310, "GAGGG": 353274, "GTAAA": 853548, "GTAAT": 512138, "GTAAC": 315247, "GTAAG": 12957, "GTATA": 607919, 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143939, "GCAAG": 6356, "GCATA": 252217, "GCATT": 187317, "GCATC": 107270, "GCATG": 5664, "GCACA": 138068, "GCACT": 100312, "GCACC": 59367, "GCACG": 35839, "GCAGA": 8390, "GCAGT": 7440, "GCAGC": 5114, "GCAGG": 6816, "GCTAA": 282384, "GCTAT": 251961, "GCTAC": 119917, "GCTAG": 2922, "GCTTA": 178161, "GCTTT": 194242, "GCTTC": 120232, "GCTTG": 4952, "GCTCA": 123185, "GCTCT": 120647, "GCTCC": 56553, "GCTCG": 33079, "GCTGA": 6642, "GCTGT": 381387, "GCTGC": 4808, "GCTGG": 7913, "GCCAA": 176552, "GCCAT": 169323, "GCCAC": 84398, "GCCAG": 5864, "GCCTA": 122347, "GCCTT": 88450, "GCCTC": 57432, "GCCTG": 6949, "GCCCA": 87284, "GCCCT": 66315, "GCCCC": 68476, "GCCCG": 32795, "GCCGA": 53883, "GCCGT": 394074, "GCCGC": 20503, "GCCGG": 2466, "GCGAA": 103589, "GCGAT": 75027, "GCGAC": 42554, "GCGAG": 3079, "GCGTA": 62836, "GCGTT": 40719, "GCGTC": 407769, "GCGTG": 3836, "GCGCA": 33205, "GCGCT": 26130, "GCGCC": 24707, "GCGCG": 19068, "GCGGA": 3426, "GCGGT": 2111, "GCGGC": 2454, "GCGGG": 3942, "GGAAA": 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21196, "GGGGG": 1181578 }, "overrepresented_sequences": {} }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/Qui2 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/Qui2 (paired)_R2.fastq.gz -o output/13-wgbs/trimmed/Qui2_R1.fq.gz -O output/13-wgbs/trimmed/Qui2_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs/fastp/Qui2.fastp.json --html output/13-wgbs/fastp/Qui2.fastp.html " }, "Qui3 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 84275732, "total_bases": 12725635532, "q20_bases": 12567504402, "q30_bases": 12168953674, "q20_rate": 0.987574, "q30_rate": 0.956255, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.212825 }, "after_filtering": { "total_reads": 82892660, "total_bases": 11447336864, "q20_bases": 11346828076, "q30_bases": 11038498745, "q20_rate": 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1684052, "CCAGC": 14551, "CCAGG": 15829, "CCTAA": 48371, "CCTAT": 45057, "CCTAC": 46110, "CCTAG": 363177, "CCTTA": 93585, "CCTTT": 511666, "CCTTC": 646133, "CCTTG": 31514, "CCTCA": 355700, "CCTCT": 756066, "CCTCC": 1100872, "CCTCG": 11083, "CCTGA": 28669, "CCTGT": 28865, "CCTGC": 11273, "CCTGG": 13908, "CCCAA": 107873, "CCCAT": 52388, "CCCAC": 75544, "CCCAG": 1823609, "CCCTA": 294261, "CCCTT": 417074, "CCCTC": 798074, "CCCTG": 18817, "CCCCA": 1158998, "CCCCT": 508446, "CCCCC": 898938, "CCCCG": 18565, "CCCGA": 21791, "CCCGT": 18382, "CCCGC": 8924, "CCCGG": 6524, "CCGAA": 24459, "CCGAT": 127929, "CCGAC": 9901, "CCGAG": 12386, "CCGTA": 13723, "CCGTT": 31259, "CCGTC": 28072, "CCGTG": 9439, "CCGCA": 8377, "CCGCT": 10189, "CCGCC": 9850, "CCGCG": 3366, "CCGGA": 14099, "CCGGT": 11791, "CCGGC": 3673, "CCGGG": 7527, "CGAAA": 1002038, "CGAAT": 970772, "CGAAC": 60321, "CGAAG": 454740, "CGATA": 1273391, "CGATT": 1521200, "CGATC": 234624, "CGATG": 542830, "CGACA": 10435, "CGACT": 15752, "CGACC": 10550, "CGACG": 193788, "CGAGA": 607644, "CGAGT": 699089, "CGAGC": 44439, "CGAGG": 314691, "CGTAA": 1124691, "CGTAT": 1499185, "CGTAC": 107971, "CGTAG": 535766, "CGTTA": 1621692, "CGTTT": 2395675, "CGTTC": 182050, "CGTTG": 644446, "CGTCA": 28053, "CGTCT": 2322977, "CGTCC": 31634, "CGTCG": 279855, "CGTGA": 499123, "CGTGT": 867848, "CGTGC": 44121, "CGTGG": 293896, "CGCAA": 9803, "CGCAT": 11315, "CGCAC": 8327, "CGCAG": 7981, "CGCTA": 9227, "CGCTT": 20330, "CGCTC": 20516, "CGCTG": 8833, "CGCCA": 6062, "CGCCT": 12795, "CGCCC": 12448, "CGCCG": 3475, "CGCGA": 153073, "CGCGT": 209160, "CGCGC": 24538, "CGCGG": 106695, "CGGAA": 3893610, "CGGAT": 808722, "CGGAC": 87729, "CGGAG": 315772, "CGGTA": 682712, "CGGTT": 1030206, "CGGTC": 90022, "CGGTG": 365572, "CGGCA": 6744, "CGGCT": 24551, "CGGCC": 6661, "CGGCG": 108695, "CGGGA": 313884, "CGGGT": 492460, "CGGGC": 38824, "CGGGG": 313117, "GAAAA": 24551930, "GAAAT": 26875650, "GAAAC": 266656, "GAAAG": 9611074, "GAATA": 19128569, "GAATT": 25158885, 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3107164, "GTAAA": 22983271, "GTAAT": 23857160, "GTAAC": 248706, "GTAAG": 8754376, "GTATA": 29734401, "GTATT": 36526121, "GTATC": 524233, "GTATG": 14686692, "GTACA": 80418, "GTACT": 91586, "GTACC": 35711, "GTACG": 690557, "GTAGA": 10612500, "GTAGT": 12684482, "GTAGC": 138793, "GTAGG": 5517302, "GTTAA": 23395176, "GTTAT": 35365880, "GTTAC": 394018, "GTTAG": 11989977, "GTTTA": 32907014, "GTTTT": 58250035, "GTTTC": 941334, "GTTTG": 20611774, "GTTCA": 164339, "GTTCT": 356364, "GTTCC": 212945, "GTTCG": 1074981, "GTTGA": 13761915, "GTTGT": 19279999, "GTTGC": 178617, "GTTGG": 6994689, "GTCAA": 119839, "GTCAT": 143971, "GTCAC": 1573614, "GTCAG": 57296, "GTCTA": 115307, "GTCTT": 319931, "GTCTC": 200209, "GTCTG": 2252910, "GTCCA": 22208, "GTCCT": 185330, "GTCCC": 223674, "GTCCG": 11560, "GTCGA": 701749, "GTCGT": 938397, "GTCGC": 51963, "GTCGG": 529433, "GTGAA": 11126053, "GTGAT": 14645412, "GTGAC": 194680, "GTGAG": 5090580, "GTGTA": 15815037, "GTGTT": 20028121, "GTGTC": 314757, "GTGTG": 10814842, 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"GGGAT": 5808740, "GGGAC": 87586, "GGGAG": 2944687, "GGGTA": 5557149, "GGGTT": 9520337, "GGGTC": 223840, "GGGTG": 3847800, "GGGCA": 41766, "GGGCT": 54991, "GGGCC": 32072, "GGGCG": 419298, "GGGGA": 3400967, "GGGGT": 5602869, "GGGGC": 334050, "GGGGG": 54571211 }, "overrepresented_sequences": {} }, "read2_before_filtering": { "total_reads": 42137866, "total_bases": 6362817766, "q20_bases": 6279270768, "q30_bases": 6076434833, "q40_bases": 6076434833, "total_cycles": 151, "quality_curves": { "A": [ 39.7299, 39.546, 39.48, 39.6176, 39.3989, 39.5952, 39.6316, 39.6695, 39.6322, 39.6385, 39.6268, 39.6389, 39.6221, 39.5766, 39.6493, 39.5821, 39.5615, 39.5635, 39.579, 39.6289, 39.4758, 39.5756, 39.5709, 39.5559, 39.4962, 39.5841, 39.5669, 39.6065, 39.5148, 39.5003, 39.553, 39.5038, 39.5552, 39.5146, 39.3482, 39.4777, 39.4466, 39.5628, 39.3356, 39.4944, 39.4125, 39.4736, 39.4551, 39.4683, 39.5301, 39.3649, 39.445, 39.3285, 39.4961, 39.4422, 39.4242, 39.4687, 39.4575, 39.4559, 39.4354, 39.4142, 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37464, "CACTG": 12210, "CACCA": 31331, "CACCT": 19554, "CACCC": 35087, "CACCG": 5272, "CACGA": 11417, "CACGT": 440136, "CACGC": 5080, "CACGG": 5897, "CAGAA": 73547, "CAGAT": 94405, "CAGAC": 10361, "CAGAG": 35389, "CAGTA": 66567, "CAGTT": 121242, "CAGTC": 428917, "CAGTG": 46690, "CAGCA": 8245, "CAGCT": 9561, "CAGCC": 6687, "CAGCG": 3795, "CAGGA": 32397, "CAGGT": 48789, "CAGGC": 5370, "CAGGG": 26175, "CTAAA": 155125, "CTAAT": 129729, "CTAAC": 442745, "CTAAG": 45728, "CTATA": 168634, "CTATT": 212619, "CTATC": 32325, "CTATG": 80537, "CTACA": 21778, "CTACT": 31191, "CTACC": 18254, "CTACG": 7858, "CTAGA": 59946, "CTAGT": 83184, "CTAGC": 5738, "CTAGG": 85083, "CTTAA": 151097, "CTTAT": 179371, "CTTAC": 26950, "CTTAG": 62921, "CTTTA": 217739, "CTTTT": 1028181, "CTTTC": 542019, "CTTTG": 152917, "CTTCA": 79788, "CTTCT": 559608, "CTTCC": 832494, "CTTCG": 20094, "CTTGA": 90036, "CTTGT": 138156, "CTTGC": 12980, "CTTGG": 53208, "CTCAA": 63534, "CTCAT": 37739, "CTCAC": 31610, "CTCAG": 28621, "CTCTA": 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6439, "CGCAT": 7805, "CGCAC": 3436, "CGCAG": 3480, "CGCTA": 5919, "CGCTT": 13267, "CGCTC": 10143, "CGCTG": 4029, "CGCCA": 2631, "CGCCT": 6384, "CGCCC": 6735, "CGCCG": 2294, "CGCGA": 139659, "CGCGT": 190046, "CGCGC": 21117, "CGCGG": 96858, "CGGAA": 553413, "CGGAT": 739502, "CGGAC": 77885, "CGGAG": 279884, "CGGTA": 624758, "CGGTT": 907386, "CGGTC": 77468, "CGGTG": 333111, "CGGCA": 3296, "CGGCT": 12746, "CGGCC": 4420, "CGGCG": 98372, "CGGGA": 283143, "CGGGT": 431581, "CGGGC": 34294, "CGGGG": 275659, "GAAAA": 22472956, "GAAAT": 24811113, "GAAAC": 232772, "GAAAG": 8670102, "GAATA": 17620732, "GAATT": 23188924, "GAATC": 324106, "GAATG": 8760807, "GAACA": 54136, "GAACT": 497261, "GAACC": 33853, "GAACG": 347348, "GAAGA": 8335651, "GAAGT": 9407715, "GAAGC": 109169, "GAAGG": 3699631, "GATAA": 18142841, "GATAT": 27024123, "GATAC": 262182, "GATAG": 9927464, "GATTA": 18989351, "GATTT": 33602357, "GATTC": 543847, "GATTG": 11645940, "GATCA": 68378, "GATCT": 211183, "GATCC": 165274, "GATCG": 641447, 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53320763, "GTTTC": 845777, "GTTTG": 18805114, "GTTCA": 137493, "GTTCT": 308152, "GTTCC": 190518, "GTTCG": 980200, "GTTGA": 12641427, "GTTGT": 17695690, "GTTGC": 159828, "GTTGG": 6373878, "GTCAA": 102122, "GTCAT": 123082, "GTCAC": 429872, "GTCAG": 45606, "GTCTA": 89360, "GTCTT": 247916, "GTCTC": 176963, "GTCTG": 471833, "GTCCA": 11614, "GTCCT": 159455, "GTCCC": 197450, "GTCCG": 7486, "GTCGA": 635987, "GTCGT": 838993, "GTCGC": 46265, "GTCGG": 474011, "GTGAA": 10206778, "GTGAT": 13426033, "GTGAC": 175758, "GTGAG": 4642754, "GTGTA": 14512749, "GTGTT": 18301166, "GTGTC": 269614, "GTGTG": 9833579, "GTGCA": 35948, "GTGCT": 51593, "GTGCC": 20349, "GTGCG": 251435, "GTGGA": 5143330, "GTGGT": 7087692, "GTGGC": 97173, "GTGGG": 3269357, "GCAAA": 56701, "GCAAT": 56088, "GCAAC": 8329, "GCAAG": 22248, "GCATA": 57972, "GCATT": 83235, "GCATC": 9360, "GCATG": 34394, "GCACA": 439365, "GCACT": 8444, "GCACC": 7295, "GCACG": 3901, "GCAGA": 26192, "GCAGT": 36319, "GCAGC": 5865, "GCAGG": 17674, "GCTAA": 41085, 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5513545550, "q40_bases": 5513545550, "total_cycles": 141, "quality_curves": { "A": [ 39.6659, 39.6732, 39.6609, 39.6124, 39.6876, 39.6198, 39.5991, 39.6003, 39.6172, 39.6668, 39.5245, 39.6186, 39.6211, 39.6099, 39.5517, 39.6369, 39.6258, 39.6704, 39.5868, 39.5716, 39.6246, 39.5772, 39.6278, 39.5876, 39.4329, 39.567, 39.5268, 39.6424, 39.4268, 39.5787, 39.5074, 39.5707, 39.5454, 39.5642, 39.6256, 39.4724, 39.5527, 39.4381, 39.5969, 39.5491, 39.5304, 39.5824, 39.5751, 39.5707, 39.5504, 39.5336, 39.5269, 39.5557, 39.5553, 39.5217, 39.5618, 39.4984, 39.4774, 39.4916, 39.4061, 39.504, 39.4228, 39.253, 39.4165, 39.362, 39.1811, 39.3832, 39.3268, 39.4101, 39.2391, 39.0875, 39.3752, 39.3628, 39.4469, 39.4118, 39.3832, 39.3931, 39.3395, 39.2472, 39.3207, 39.3904, 39.3115, 39.4073, 39.3279, 39.302, 39.2635, 39.2931, 39.3134, 39.2855, 39.2605, 39.2693, 39.2734, 39.2411, 39.21, 39.3059, 39.2974, 39.2208, 39.2302, 39.2685, 39.1897, 39.2585, 39.1523, 38.9646, 39.2313, 39.1795, 39.1622, 39.1454, 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6532725, "TCCTC": 4134532, "TCCTG": 36618, "TCCCA": 5104552, "TCCCT": 3712322, "TCCCC": 2881667, "TCCCG": 274545, "TCCGA": 897844, "TCCGT": 564383, "TCCGC": 245976, "TCCGG": 2234, "TCGAA": 1792891, "TCGAT": 1179722, "TCGAC": 620525, "TCGAG": 3393, "TCGTA": 1036177, "TCGTT": 683051, "TCGTC": 442453, "TCGTG": 421913, "TCGCA": 431368, "TCGCT": 400802, "TCGCC": 659040, "TCGCG": 137524, "TCGGA": 33791, "TCGGT": 411501, "TCGGC": 2327, "TCGGG": 8443, "TGAAA": 204888, "TGAAT": 112691, "TGAAC": 58185, "TGAAG": 17873, "TGATA": 125757, "TGATT": 86297, "TGATC": 43764, "TGATG": 5118, "TGACA": 67492, "TGACT": 37344, "TGACC": 33200, "TGACG": 5028, "TGAGA": 24881, "TGAGT": 6518, "TGAGC": 3481, "TGAGG": 28351, "TGTAA": 98314, "TGTAT": 94032, "TGTAC": 38882, "TGTAG": 782942, "TGTTA": 65452, "TGTTT": 85789, "TGTTC": 44087, "TGTTG": 6397, "TGTCA": 438917, "TGTCT": 35035, "TGTCC": 48046, "TGTCG": 50992, "TGTGA": 5241, "TGTGT": 12291, "TGTGC": 9881, "TGTGG": 10009, "TGCAA": 47144, "TGCAT": 40412, "TGCAC": 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23169, "CATGG": 383984, "CACAA": 19045814, "CACAT": 13290402, "CACAC": 10072336, "CACAG": 23119, "CACTA": 13109585, "CACTT": 8749418, "CACTC": 5487514, "CACTG": 28587, "CACCA": 8012006, "CACCT": 5386373, "CACCC": 3488031, "CACCG": 346935, "CACGA": 762164, "CACGT": 490020, "CACGC": 244725, "CACGG": 11573, "CAGAA": 46991, "CAGAT": 47649, "CAGAC": 21009, "CAGAG": 6372, "CAGTA": 28158, "CAGTT": 25526, "CAGTC": 17668, "CAGTG": 10312, "CAGCA": 17875, "CAGCT": 13654, "CAGCC": 11221, "CAGCG": 2090, "CAGGA": 6172, "CAGGT": 3541, "CAGGC": 3443, "CAGGG": 4638, "CTAAA": 30658295, "CTAAT": 19691411, "CTAAC": 11174376, "CTAAG": 29292, "CTATA": 25826408, "CTATT": 17115010, "CTATC": 9860000, "CTATG": 46312, "CTACA": 13251798, "CTACT": 8082090, "CTACC": 4841932, "CTACG": 477553, "CTAGA": 34841, "CTAGT": 22322, "CTAGC": 9934, "CTAGG": 16110, "CTTAA": 19128113, "CTTAT": 14439100, "CTTAC": 8016982, "CTTAG": 19203, "CTTTA": 18400955, "CTTTT": 15992479, "CTTTC": 8622689, "CTTTG": 32540, "CTTCA": 9666630, 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"CCTTA": 8088181, "CCTTT": 7756489, "CCTTC": 3673329, "CCTTG": 17662, "CCTCA": 4478579, "CCTCT": 4193509, "CCTCC": 2581457, "CCTCG": 276696, "CCTGA": 27184, "CCTGT": 19777, "CCTGC": 10326, "CCTGG": 24599, "CCCAA": 7842659, "CCCAT": 4807858, "CCCAC": 3471756, "CCCAG": 22202, "CCCTA": 5724253, "CCCTT": 3784327, "CCCTC": 2525237, "CCCTG": 18407, "CCCCA": 4117166, "CCCCT": 3118027, "CCCCC": 5863459, "CCCCG": 263841, "CCCGA": 533504, "CCCGT": 251975, "CCCGC": 191712, "CCCGG": 5539, "CCGAA": 1202640, "CCGAT": 835815, "CCGAC": 478920, "CCGAG": 3159, "CCGTA": 1027647, "CCGTT": 415023, "CCGTC": 346444, "CCGTG": 3770, "CCGCA": 241599, "CCGCT": 236592, "CCGCC": 214282, "CCGCG": 95304, "CCGGA": 3804, "CCGGT": 4978, "CCGGC": 1886, "CCGGG": 1711, "CGAAA": 2788114, "CGAAT": 1570459, "CGAAC": 976227, "CGAAG": 7501, "CGATA": 1640699, "CGATT": 1054936, "CGATC": 784613, "CGATG": 5133, "CGACA": 736014, "CGACT": 538568, "CGACC": 432017, "CGACG": 248783, "CGAGA": 5745, "CGAGT": 3377, "CGAGC": 2538, "CGAGG": 2755, "CGTAA": 1438663, "CGTAT": 1507037, "CGTAC": 626046, "CGTAG": 3602, "CGTTA": 881603, "CGTTT": 786514, "CGTTC": 348780, "CGTTG": 4001, "CGTCA": 581405, "CGTCT": 448116, "CGTCC": 278318, "CGTCG": 586909, "CGTGA": 4776, "CGTGT": 424592, "CGTGC": 2977, "CGTGG": 3402, "CGCAA": 605908, "CGCAT": 406756, "CGCAC": 247019, "CGCAG": 3129, "CGCTA": 513851, "CGCTT": 372399, "CGCTC": 234115, "CGCTG": 2312, "CGCCA": 324533, "CGCCT": 199523, "CGCCC": 187631, "CGCCG": 485803, "CGCGA": 218968, "CGCGT": 122376, "CGCGC": 97837, "CGCGG": 1557, "CGGAA": 37663, "CGGAT": 3383, "CGGAC": 2579, "CGGAG": 2994, "CGGTA": 2914, "CGGTT": 4082, "CGGTC": 2419, "CGGTG": 420611, "CGGCA": 2174, "CGGCT": 1943, "CGGCC": 2084, "CGGCG": 1980, "CGGGA": 9087, "CGGGT": 1320, "CGGGC": 1358, "CGGGG": 3733, "GAAAA": 2719186, "GAAAT": 1491473, "GAAAC": 778557, "GAAAG": 819239, "GAATA": 1341349, "GAATT": 1013328, "GAATC": 614501, "GAATG": 23204, "GAACA": 707276, "GAACT": 456667, "GAACC": 344477, "GAACG": 148828, "GAAGA": 803970, "GAAGT": 66212, "GAAGC": 53666, "GAAGG": 407024, "GATAA": 1387946, "GATAT": 1050046, "GATAC": 551358, "GATAG": 27755, "GATTA": 891551, "GATTT": 808670, "GATTC": 391898, "GATTG": 4673, "GATCA": 573888, "GATCT": 890452, "GATCC": 242241, "GATCG": 131765, "GATGA": 28043, "GATGT": 6662, "GATGC": 3972, "GATGG": 24252, "GACAA": 679034, "GACAT": 429330, "GACAC": 249819, "GACAG": 4891, "GACTA": 463045, "GACTT": 321670, "GACTC": 167915, "GACTG": 3247, "GACCA": 327695, "GACCT": 196074, "GACCC": 170790, "GACCG": 76401, "GACGA": 136283, "GACGT": 93166, "GACGC": 54491, "GACGG": 2511, "GAGAA": 901255, "GAGAT": 445412, "GAGAC": 192528, "GAGAG": 607336, "GAGTA": 85085, "GAGTT": 64465, "GAGTC": 44590, "GAGTG": 415648, "GAGCA": 62526, "GAGCT": 53265, "GAGCC": 41279, "GAGCG": 138506, "GAGGA": 697082, "GAGGT": 59634, "GAGGC": 46711, "GAGGG": 382965, "GTAAA": 923231, "GTAAT": 548032, "GTAAC": 341684, "GTAAG": 11927, "GTATA": 648511, "GTATT": 495635, "GTATC": 649077, "GTATG": 4747, "GTACA": 347459, "GTACT": 210366, "GTACC": 141171, "GTACG": 91877, "GTAGA": 383878, "GTAGT": 5334, "GTAGC": 2629, "GTAGG": 418080, "GTTAA": 499822, "GTTAT": 431994, "GTTAC": 215814, "GTTAG": 3756, "GTTTA": 422431, "GTTTT": 399336, "GTTTC": 217006, "GTTTG": 5976, "GTTCA": 227457, "GTTCT": 154236, "GTTCC": 107675, "GTTCG": 39108, "GTTGA": 4590, "GTTGT": 9071, "GTTGC": 11510, "GTTGG": 4478, "GTCAA": 721792, "GTCAT": 303623, "GTCAC": 186079, "GTCAG": 3531, "GTCTA": 265829, "GTCTT": 173514, "GTCTC": 130221, "GTCTG": 3639, "GTCCA": 152590, "GTCCT": 102095, "GTCCC": 83279, "GTCCG": 72431, "GTCGA": 116751, "GTCGT": 496358, "GTCGC": 431952, "GTCGG": 9188, "GTGAA": 12043, "GTGAT": 5924, "GTGAC": 3636, "GTGAG": 10876, "GTGTA": 790129, "GTGTT": 9241, "GTGTC": 465948, "GTGTG": 12630, "GTGCA": 3689, "GTGCT": 15363, "GTGCC": 2845, "GTGCG": 1522, "GTGGA": 11868, "GTGGT": 421621, "GTGGC": 3138, "GTGGG": 17958, "GCAAA": 429362, "GCAAT": 275296, "GCAAC": 155394, "GCAAG": 4409, "GCATA": 267670, "GCATT": 193371, "GCATC": 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392605, "GGATT": 296705, "GGATC": 189931, "GGATG": 23518, "GGACA": 168527, "GGACT": 115544, "GGACC": 92521, "GGACG": 9615, "GGAGA": 663695, "GGAGT": 59289, "GGAGC": 46191, "GGAGG": 409842, "GGTAA": 73440, "GGTAT": 43535, "GGTAC": 30755, "GGTAG": 10874, "GGTTA": 54216, "GGTTT": 41646, "GGTTC": 26523, "GGTTG": 14411, "GGTCA": 47109, "GGTCT": 21089, "GGTCC": 18739, "GGTCG": 408776, "GGTGA": 10654, "GGTGT": 418393, "GGTGC": 2877, "GGTGG": 424744, "GGCAA": 55190, "GGCAT": 32415, "GGCAC": 20986, "GGCAG": 4392, "GGCTA": 49628, "GGCTT": 29066, "GGCTC": 21641, "GGCTG": 3405, "GGCCA": 36870, "GGCCT": 19700, "GGCCC": 20426, "GGCCG": 3926, "GGCGA": 4905, "GGCGT": 3242, "GGCGC": 2659, "GGCGG": 3553, "GGGAA": 895975, "GGGAT": 237645, "GGGAC": 95849, "GGGAG": 388463, "GGGTA": 44055, "GGGTT": 34804, "GGGTC": 23516, "GGGTG": 18946, "GGGCA": 29109, "GGGCT": 26599, "GGGCC": 20717, "GGGCG": 4502, "GGGGA": 407042, "GGGGT": 39818, "GGGGC": 21751, "GGGGG": 1414489 }, "overrepresented_sequences": {} }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/Qui3 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/Qui3 (paired)_R2.fastq.gz -o output/13-wgbs/trimmed/Qui3_R1.fq.gz -O output/13-wgbs/trimmed/Qui3_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs/fastp/Qui3.fastp.json --html output/13-wgbs/fastp/Qui3.fastp.html " }, "Qui4 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 70096496, "total_bases": 10584570896, "q20_bases": 10450858325, "q30_bases": 10097793159, "q20_rate": 0.987367, "q30_rate": 0.954011, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.215619 }, "after_filtering": { "total_reads": 68887774, "total_bases": 9501368786, "q20_bases": 9416611615, "q30_bases": 9142305416, "q20_rate": 0.991079, "q30_rate": 0.962209, "read1_mean_length": 137, 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"CCTTG": 23883, "CCTCA": 316231, "CCTCT": 598036, "CCTCC": 925686, "CCTCG": 8433, "CCTGA": 18929, "CCTGT": 21693, "CCTGC": 7436, "CCTGG": 10408, "CCCAA": 83039, "CCCAT": 33032, "CCCAC": 42538, "CCCAG": 1809637, "CCCTA": 273112, "CCCTT": 316645, "CCCTC": 654473, "CCCTG": 13993, "CCCCA": 1145044, "CCCCT": 415096, "CCCCC": 759781, "CCCCG": 14755, "CCCGA": 17747, "CCCGT": 14833, "CCCGC": 5162, "CCCGG": 7028, "CCGAA": 20805, "CCGAT": 66497, "CCGAC": 6252, "CCGAG": 11482, "CCGTA": 10611, "CCGTT": 22954, "CCGTC": 20700, "CCGTG": 7973, "CCGCA": 5988, "CCGCT": 5237, "CCGCC": 5251, "CCGCG": 2573, "CCGGA": 12641, "CCGGT": 10512, "CCGGC": 2633, "CCGGG": 5856, "CGAAA": 762392, "CGAAT": 757080, "CGAAC": 47680, "CGAAG": 358279, "CGATA": 1002170, "CGATT": 1219010, "CGATC": 1204142, "CGATG": 442101, "CGACA": 7374, "CGACT": 11554, "CGACC": 8076, "CGACG": 152925, "CGAGA": 479535, "CGAGT": 565692, "CGAGC": 34703, "CGAGG": 253836, "CGTAA": 877776, "CGTAT": 1198817, "CGTAC": 84408, "CGTAG": 429907, "CGTTA": 1295867, "CGTTT": 1877083, "CGTTC": 145965, "CGTTG": 528904, "CGTCA": 23483, "CGTCT": 2078939, "CGTCC": 21862, "CGTCG": 228179, "CGTGA": 409422, "CGTGT": 726174, "CGTGC": 37332, "CGTGG": 247517, "CGCAA": 7253, "CGCAT": 8206, "CGCAC": 5405, "CGCAG": 6761, "CGCTA": 6709, "CGCTT": 14712, "CGCTC": 11203, "CGCTG": 7510, "CGCCA": 3753, "CGCCT": 8701, "CGCCC": 7853, "CGCCG": 2440, "CGCGA": 121495, "CGCGT": 167251, "CGCGC": 18492, "CGCGG": 85776, "CGGAA": 3509487, "CGGAT": 645246, "CGGAC": 69521, "CGGAG": 251345, "CGGTA": 1813416, "CGGTT": 802331, "CGGTC": 72333, "CGGTG": 306710, "CGGCA": 6123, "CGGCT": 9636, "CGGCC": 4829, "CGGCG": 87771, "CGGGA": 251001, "CGGGT": 396783, "CGGGC": 31376, "CGGGG": 262889, "GAAAA": 19380520, "GAAAT": 21736551, "GAAAC": 199741, "GAAAG": 7750444, "GAATA": 15581166, "GAATT": 20832429, "GAATC": 293594, "GAATG": 7963850, "GAACA": 57110, "GAACT": 1825202, "GAACC": 39756, "GAACG": 301451, "GAAGA": 10244460, "GAAGT": 8616116, "GAAGC": 99763, "GAAGG": 3511879, "GATAA": 15916886, "GATAT": 24316378, "GATAC": 235431, "GATAG": 8886244, "GATTA": 17239696, "GATTT": 30676598, "GATTC": 504543, "GATTG": 10883373, "GATCA": 69452, "GATCT": 1263977, "GATCC": 170840, "GATCG": 3916790, "GATGA": 8381486, "GATGT": 12720887, "GATGC": 100772, "GATGG": 4627076, "GACAA": 47296, "GACAT": 57130, "GACAC": 12338, "GACAG": 26483, "GACTA": 37991, "GACTT": 93695, "GACTC": 48606, "GACTG": 25430, "GACCA": 13185, "GACCT": 34880, "GACCC": 47104, "GACCG": 7134, "GACGA": 390148, "GACGT": 591981, "GACGC": 25227, "GACGG": 307833, "GAGAA": 7244917, "GAGAT": 8141430, "GAGAC": 102533, "GAGAG": 8520376, "GAGTA": 6732831, "GAGTT": 9770117, "GAGTC": 144093, "GAGTG": 5495345, "GAGCA": 2616807, "GAGCT": 36257, "GAGCC": 27089, "GAGCG": 214872, "GAGGA": 3935776, "GAGGT": 4615958, "GAGGC": 56220, "GAGGG": 2717372, "GTAAA": 18659718, "GTAAT": 19769160, "GTAAC": 199403, "GTAAG": 7332786, "GTATA": 25261854, "GTATT": 30856044, "GTATC": 454608, "GTATG": 12730246, "GTACA": 1271710, "GTACT": 81543, "GTACC": 35033, "GTACG": 556387, "GTAGA": 8893288, "GTAGT": 10934360, "GTAGC": 119117, "GTAGG": 4710236, "GTTAA": 19417286, "GTTAT": 29831273, "GTTAC": 328544, "GTTAG": 10183863, "GTTTA": 27797724, "GTTTT": 49385988, "GTTTC": 805009, "GTTTG": 17846909, "GTTCA": 133614, "GTTCT": 314970, "GTTCC": 203426, "GTTCG": 891072, "GTTGA": 11712518, "GTTGT": 16851090, "GTTGC": 153422, "GTTGG": 6092261, "GTCAA": 99112, "GTCAT": 120640, "GTCAC": 1409858, "GTCAG": 47257, "GTCTA": 100193, "GTCTT": 245583, "GTCTC": 183848, "GTCTG": 2035469, "GTCCA": 16700, "GTCCT": 162445, "GTCCC": 219388, "GTCCG": 9755, "GTCGA": 564483, "GTCGT": 758755, "GTCGC": 42538, "GTCGG": 431084, "GTGAA": 9340892, "GTGAT": 12733251, "GTGAC": 162847, "GTGAG": 4583986, "GTGTA": 13951826, "GTGTT": 17554770, "GTGTC": 269224, "GTGTG": 10289879, "GTGCA": 35750, "GTGCT": 55649, "GTGCC": 22011, "GTGCG": 232615, "GTGGA": 4803547, "GTGGT": 6923622, "GTGGC": 90681, "GTGGG": 3254865, "GCAAA": 57112, "GCAAT": 54306, "GCAAC": 11485, "GCAAG": 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179932, "TCATT": 308436, "TCATC": 37539, "TCATG": 107663, "TCACA": 17959, "TCACT": 58057, "TCACC": 29852, "TCACG": 309375, "TCAGA": 79253, "TCAGT": 111191, "TCAGC": 7808, "TCAGG": 48182, "TCTAA": 154915, "TCTAT": 247213, "TCTAC": 23608, "TCTAG": 91283, "TCTTA": 193858, "TCTTT": 858787, "TCTTC": 730261, "TCTTG": 141012, "TCTCA": 43694, "TCTCT": 765046, "TCTCC": 1180001, "TCTCG": 22176, "TCTGA": 378785, "TCTGT": 141164, "TCTGC": 10212, "TCTGG": 333879, "TCCAA": 30822, "TCCAT": 23627, "TCCAC": 15662, "TCCAG": 307280, "TCCTA": 31798, "TCCTT": 522587, "TCCTC": 1004343, "TCCTG": 20076, "TCCCA": 59032, "TCCCT": 647660, "TCCCC": 1273023, "TCCCG": 7286, "TCCGA": 16600, "TCCGT": 19858, "TCCGC": 5111, "TCCGG": 8008, "TCGAA": 870853, "TCGAT": 1288176, "TCGAC": 67418, "TCGAG": 649969, "TCGTA": 1132833, "TCGTT": 1789933, "TCGTC": 119996, "TCGTG": 665005, "TCGCA": 7264, "TCGCT": 11100, "TCGCC": 5839, "TCGCG": 178988, "TCGGA": 643285, "TCGGT": 845023, "TCGGC": 45878, "TCGGG": 454629, "TGAAA": 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40525, "CAGCA": 6233, "CAGCT": 6056, "CAGCC": 4861, "CAGCG": 3239, "CAGGA": 26393, "CAGGT": 39680, "CAGGC": 3724, "CAGGG": 22201, "CTAAA": 124497, "CTAAT": 107785, "CTAAC": 18111, "CTAAG": 37226, "CTATA": 139720, "CTATT": 178675, "CTATC": 23054, "CTATG": 68231, "CTACA": 12962, "CTACT": 22848, "CTACC": 11248, "CTACG": 5929, "CTAGA": 50458, "CTAGT": 60607, "CTAGC": 4394, "CTAGG": 34175, "CTTAA": 124112, "CTTAT": 147723, "CTTAC": 20574, "CTTAG": 53338, "CTTTA": 180108, "CTTTT": 808619, "CTTTC": 437384, "CTTTG": 129089, "CTTCA": 62542, "CTTCT": 427803, "CTTCC": 695948, "CTTCG": 16961, "CTTGA": 75560, "CTTGT": 117989, "CTTGC": 10720, "CTTGG": 45053, "CTCAA": 49907, "CTCAT": 28812, "CTCAC": 23800, "CTCAG": 18106, "CTCTA": 31824, "CTCTT": 467887, "CTCTC": 793005, "CTCTG": 15669, "CTCCA": 313145, "CTCCT": 577048, "CTCCC": 1021616, "CTCCG": 5860, "CTCGA": 11602, "CTCGT": 15837, "CTCGC": 3827, "CTCGG": 6419, "CTGAA": 358490, "CTGAT": 60120, "CTGAC": 9513, "CTGAG": 24246, "CTGTA": 74485, "CTGTT": 111672, "CTGTC": 18268, "CTGTG": 45600, "CTGCA": 6567, "CTGCT": 9672, "CTGCC": 4922, "CTGCG": 2972, "CTGGA": 30276, "CTGGT": 40068, "CTGGC": 4111, "CTGGG": 308666, "CCAAA": 59707, "CCAAT": 19590, "CCAAC": 28608, "CCAAG": 10729, "CCATA": 12442, "CCATT": 27645, "CCATC": 14900, "CCATG": 7896, "CCACA": 11064, "CCACT": 25853, "CCACC": 20156, "CCACG": 4872, "CCAGA": 27644, "CCAGT": 302873, "CCAGC": 5052, "CCAGG": 6505, "CCTAA": 20520, "CCTAT": 18543, "CCTAC": 11545, "CCTAG": 8529, "CCTTA": 21490, "CCTTT": 326686, "CCTTC": 451376, "CCTTG": 16886, "CCTCA": 34681, "CCTCT": 545297, "CCTCC": 841028, "CCTCG": 4689, "CCTGA": 10903, "CCTGT": 15176, "CCTGC": 4796, "CCTGG": 7302, "CCCAA": 44798, "CCCAT": 19121, "CCCAC": 24378, "CCCAG": 27350, "CCCTA": 15816, "CCCTT": 268141, "CCCTC": 588919, "CCCTG": 8213, "CCCCA": 50530, "CCCCT": 356022, "CCCCC": 636804, "CCCCG": 5567, "CCCGA": 6731, "CCCGT": 6587, "CCCGC": 2501, "CCCGG": 3141, "CCGAA": 11590, "CCGAT": 11834, "CCGAC": 3420, "CCGAG": 4365, "CCGTA": 6358, "CCGTT": 16287, "CCGTC": 7524, "CCGTG": 5880, "CCGCA": 2055, "CCGCT": 2705, "CCGCC": 2665, "CCGCG": 1807, "CCGGA": 5551, "CCGGT": 6303, "CCGGC": 1629, "CCGGG": 3957, "CGAAA": 687021, "CGAAT": 693297, "CGAAC": 36703, "CGAAG": 321653, "CGATA": 914478, "CGATT": 1109391, "CGATC": 381692, "CGATG": 401990, "CGACA": 5327, "CGACT": 8337, "CGACC": 5233, "CGACG": 138279, "CGAGA": 432721, "CGAGT": 519262, "CGAGC": 30262, "CGAGG": 229390, "CGTAA": 805417, "CGTAT": 1098591, "CGTAC": 75954, "CGTAG": 392996, "CGTTA": 1189863, "CGTTT": 1705471, "CGTTC": 129427, "CGTTG": 478719, "CGTCA": 13948, "CGTCT": 317800, "CGTCC": 14846, "CGTCG": 204234, "CGTGA": 372438, "CGTGT": 656135, "CGTGC": 33140, "CGTGG": 224281, "CGCAA": 5186, "CGCAT": 6244, "CGCAC": 2561, "CGCAG": 2986, "CGCTA": 4802, "CGCTT": 10332, "CGCTC": 5860, "CGCTG": 3489, "CGCCA": 1997, "CGCCT": 4785, "CGCCC": 5345, "CGCCG": 1839, "CGCGA": 110732, "CGCGT": 152086, "CGCGC": 16131, "CGCGG": 77723, "CGGAA": 425219, "CGGAT": 589136, "CGGAC": 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output/13-wgbs/fastp/Qui4.fastp.html " }, "Qui5 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 67333024, "total_bases": 10167286624, "q20_bases": 10045682745, "q30_bases": 9745491234, "q20_rate": 0.98804, "q30_rate": 0.958514, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.214439 }, "after_filtering": { "total_reads": 66298690, "total_bases": 9142705206, "q20_bases": 9065193982, "q30_bases": 8833877464, "q20_rate": 0.991522, "q30_rate": 0.966221, "read1_mean_length": 137, "read2_mean_length": 137, "gc_content": 0.189484 } }, "filtering_result": { "passed_filter_reads": 66298690, "low_quality_reads": 527506, "too_many_N_reads": 1374, "too_short_reads": 505454, "too_long_reads": 0 }, "duplication": { "rate": 0.0512097 }, "insert_size": { "peak": 189, "unknown": 650941, "histogram": [ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 2318, 0, 0, 0, 0, 0, 0, 0, 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30811, "ACCCT": 110349, "ACCCC": 138056, "ACCCG": 12452, "ACCGA": 8209, "ACCGT": 14210, "ACCGC": 3499, "ACCGG": 9633, "ACGAA": 582803, "ACGAT": 794575, "ACGAC": 69421, "ACGAG": 337527, "ACGTA": 832936, "ACGTT": 1197707, "ACGTC": 2087371, "ACGTG": 410941, "ACGCA": 6877, "ACGCT": 14962, "ACGCC": 7308, "ACGCG": 101015, "ACGGA": 470771, "ACGGT": 469200, "ACGGC": 29848, "ACGGG": 221247, "AGAAA": 15107542, "AGAAT": 14000559, "AGAAC": 142773, "AGAAG": 6023319, "AGATA": 16022335, "AGATT": 17502343, "AGATC": 3588391, "AGATG": 7454817, "AGACA": 51183, "AGACT": 54470, "AGACC": 27256, "AGACG": 369932, "AGAGA": 10099344, "AGAGT": 7393510, "AGAGC": 2646454, "AGAGG": 3842679, "AGTAA": 12893347, "AGTAT": 18412427, "AGTAC": 185848, "AGTAG": 7019151, "AGTTA": 16499156, "AGTTT": 27498399, "AGTTC": 402362, "AGTTG": 9949227, "AGTCA": 1380133, "AGTCT": 152736, "AGTCC": 112874, "AGTCG": 449404, "AGTGA": 8359409, "AGTGT": 11827024, "AGTGC": 96410, "AGTGG": 4117054, "AGCAA": 49591, "AGCAT": 56002, "AGCAC": 2394177, "AGCAG": 27155, "AGCTA": 35526, "AGCTT": 75548, "AGCTC": 48797, "AGCTG": 21681, "AGCCA": 13438, "AGCCT": 40815, "AGCCC": 50454, "AGCCG": 3366, "AGCGA": 335769, "AGCGT": 391540, "AGCGC": 27315, "AGCGG": 190570, "AGGAA": 6563441, "AGGAT": 6574392, "AGGAC": 81433, "AGGAG": 3325598, "AGGTA": 7398172, "AGGTT": 10723888, "AGGTC": 177390, "AGGTG": 4846914, "AGGCA": 26790, "AGGCT": 38744, "AGGCC": 28482, "AGGCG": 168435, "AGGGA": 3344314, "AGGGT": 4864489, "AGGGC": 80242, "AGGGG": 3635971, "TAAAA": 37994766, "TAAAT": 44271862, "TAAAC": 400308, "TAAAG": 15504301, "TAATA": 37503097, "TAATT": 47836503, "TAATC": 618369, "TAATG": 19961541, "TAACA": 119213, "TAACT": 163058, "TAACC": 64352, "TAACG": 723674, "TAAGA": 12351712, "TAAGT": 16150060, "TAAGC": 159442, "TAAGG": 7110600, "TATAA": 39410004, "TATAT": 67638211, "TATAC": 572533, "TATAG": 22445311, "TATTA": 50225159, "TATTT": 88008614, "TATTC": 1081297, "TATTG": 31547707, "TATCA": 202626, "TATCT": 1373077, "TATCC": 320099, "TATCG": 1397002, "TATGA": 19411754, "TATGT": 32332734, "TATGC": 274508, "TATGG": 10976852, "TACAA": 125465, "TACAT": 192394, "TACAC": 25091, "TACAG": 74996, "TACTA": 113402, "TACTT": 236137, "TACTC": 85797, "TACTG": 68735, "TACCA": 30272, "TACCT": 68487, "TACCC": 85242, "TACCG": 8218, "TACGA": 848189, "TACGT": 1204341, "TACGC": 58467, "TACGG": 546033, "TAGAA": 15552697, "TAGAT": 20353482, "TAGAC": 233217, "TAGAG": 8610526, "TAGTA": 18261846, "TAGTT": 24966581, "TAGTC": 360296, "TAGTG": 11828302, "TAGCA": 52178, "TAGCT": 75459, "TAGCC": 39666, "TAGCG": 435474, "TAGGA": 7196557, "TAGGT": 10581373, "TAGGC": 112832, "TAGGG": 5509915, "TTAAA": 45386610, "TTAAT": 47529758, "TTAAC": 476293, "TTAAG": 16520173, "TTATA": 55904384, "TTATT": 78047623, "TTATC": 1081360, "TTATG": 26569898, "TTACA": 175679, "TTACT": 246213, "TTACC": 92680, "TTACG": 1209154, "TTAGA": 19152907, "TTAGT": 24336016, "TTAGC": 262945, "TTAGG": 10263242, "TTTAA": 55870384, "TTTAT": 82233183, "TTTAC": 884934, "TTTAG": 26931985, "TTTTA": 87943962, "TTTTT": 164085054, "TTTTC": 3294999, "TTTTG": 52741332, "TTTCA": 693345, "TTTCT": 1589739, "TTTCC": 1138433, "TTTCG": 2480555, "TTTGA": 33546315, "TTTGT": 49005985, "TTTGC": 464385, "TTTGG": 18178957, "TTCAA": 444012, "TTCAT": 419907, "TTCAC": 105482, "TTCAG": 253955, "TTCTA": 353257, "TTCTT": 1426625, "TTCTC": 1051309, "TTCTG": 270524, "TTCCA": 247104, "TTCCT": 877553, "TTCCC": 1272297, "TTCCG": 111101, "TTCGA": 1527111, "TTCGT": 2001082, "TTCGC": 104667, "TTCGG": 1074326, "TTGAA": 24306141, "TTGAT": 31094207, "TTGAC": 304587, "TTGAG": 10157525, "TTGTA": 31493087, "TTGTT": 46484892, "TTGTC": 638203, "TTGTG": 17291921, "TTGCA": 101380, "TTGCT": 184643, "TTGCC": 66181, "TTGCG": 520321, "TTGGA": 11434146, "TTGGT": 16202603, "TTGGC": 163462, "TTGGG": 7080827, "TCAAA": 331916, "TCAAT": 259292, "TCAAC": 87030, "TCAAG": 100524, "TCATA": 219084, "TCATT": 391141, "TCATC": 66599, "TCATG": 129802, "TCACA": 33675, "TCACT": 1125374, "TCACC": 1295430, "TCACG": 18084, "TCAGA": 629709, "TCAGT": 149612, "TCAGC": 16542, "TCAGG": 60869, "TCTAA": 188232, "TCTAT": 288138, "TCTAC": 37750, "TCTAG": 654862, "TCTTA": 258118, "TCTTT": 1369689, "TCTTC": 1126066, "TCTTG": 190550, "TCTCA": 191302, "TCTCT": 912941, "TCTCC": 1310193, "TCTCG": 29561, "TCTGA": 1911253, "TCTGT": 202160, "TCTGC": 58213, "TCTGG": 567895, "TCCAA": 54835, "TCCAT": 43232, "TCCAC": 32567, "TCCAG": 2098336, "TCCTA": 160602, "TCCTT": 652745, "TCCTC": 1152698, "TCCTG": 32878, "TCCCA": 811971, "TCCCT": 738450, "TCCCC": 1334925, "TCCCG": 14700, "TCCGA": 88689, "TCCGT": 32512, "TCCGC": 10592, "TCCGG": 12275, "TCGAA": 923815, "TCGAT": 1353122, "TCGAC": 74501, "TCGAG": 682868, "TCGTA": 1186029, "TCGTT": 2004481, "TCGTC": 231453, "TCGTG": 701718, "TCGCA": 10290, "TCGCT": 15339, "TCGCC": 8345, "TCGCG": 189361, "TCGGA": 3684678, "TCGGT": 906939, "TCGGC": 58532, "TCGGG": 491028, "TGAAA": 21898286, "TGAAT": 19958330, "TGAAC": 1876501, "TGAAG": 8382398, "TGATA": 22028702, "TGATT": 28511228, "TGATC": 423916, "TGATG": 12254423, "TGACA": 65779, "TGACT": 105610, "TGACC": 50101, "TGACG": 483810, "TGAGA": 7554284, "TGAGT": 8927714, "TGAGC": 111454, "TGAGG": 4072217, "TGTAA": 22283763, "TGTAT": 35548821, "TGTAC": 321587, "TGTAG": 11737028, "TGTTA": 28390385, "TGTTT": 47283674, "TGTTC": 661667, "TGTTG": 17120620, "TGTCA": 1247843, "TGTCT": 297980, "TGTCC": 183186, "TGTCG": 830191, "TGTGA": 11669250, "TGTGT": 20167556, "TGTGC": 199881, "TGTGG": 6535317, "TGCAA": 72000, "TGCAT": 94499, "TGCAC": 12275, "TGCAG": 41178, "TGCTA": 64201, "TGCTT": 200399, "TGCTC": 60370, "TGCTG": 43700, "TGCCA": 20916, "TGCCT": 54077, "TGCCC": 60973, "TGCCG": 87580, "TGCGA": 360604, "TGCGT": 486412, "TGCGC": 30411, "TGCGG": 205052, "TGGAA": 8978119, "TGGAT": 10715497, "TGGAC": 114368, "TGGAG": 4544329, "TGGTA": 10871514, "TGGTT": 15482328, "TGGTC": 261803, "TGGTG": 7418671, "TGGCA": 42535, "TGGCT": 62035, "TGGCC": 35401, "TGGCG": 391940, "TGGGA": 4654947, "TGGGT": 6159768, "TGGGC": 88596, "TGGGG": 3884319, "CAAAA": 257655, "CAAAT": 205310, "CAAAC": 81986, "CAAAG": 81341, "CAATA": 144594, "CAATT": 215938, "CAATC": 36909, "CAATG": 80385, "CAACA": 31732, "CAACT": 72605, "CAACC": 52199, "CAACG": 16994, "CAAGA": 60302, "CAAGT": 82818, "CAAGC": 10186, "CAAGG": 38866, "CATAA": 134412, "CATAT": 218323, "CATAC": 23061, "CATAG": 73320, "CATTA": 167695, "CATTT": 375804, "CATTC": 55956, "CATTG": 112003, "CATCA": 35281, "CATCT": 68931, "CATCC": 38168, "CATCG": 12267, "CATGA": 73075, "CATGT": 129727, "CATGC": 9174, "CATGG": 46104, "CACAA": 31984, "CACAT": 37467, "CACAC": 2247915, "CACAG": 18814, "CACTA": 1035557, "CACTT": 88873, "CACTC": 49514, "CACTG": 16191, "CACCA": 41871, "CACCT": 1240537, "CACCC": 51701, "CACCG": 9103, "CACGA": 12194, "CACGT": 2084616, "CACGC": 10612, "CACGG": 6602, "CAGAA": 79752, "CAGAT": 2757018, "CAGAC": 16057, "CAGAG": 40685, "CAGTA": 70668, "CAGTT": 127499, "CAGTC": 1369669, "CAGTG": 51861, "CAGCA": 11465, "CAGCT": 18459, "CAGCC": 12425, "CAGCG": 4375, "CAGGA": 34658, "CAGGT": 52619, "CAGGC": 7617, "CAGGG": 30183, "CTAAA": 150910, "CTAAT": 131403, "CTAAC": 34020, "CTAAG": 47195, "CTATA": 166292, "CTATT": 224544, "CTATC": 993241, "CTATG": 82386, "CTACA": 23508, "CTACT": 36706, "CTACC": 23096, "CTACG": 8083, "CTAGA": 486172, "CTAGT": 396038, "CTAGC": 8176, "CTAGG": 145995, "CTTAA": 154176, "CTTAT": 183899, "CTTAC": 35250, "CTTAG": 128148, "CTTTA": 250307, "CTTTT": 1237338, "CTTTC": 548244, "CTTTG": 312579, "CTTCA": 170354, "CTTCT": 749747, "CTTCC": 875717, "CTTCG": 23936, "CTTGA": 100680, "CTTGT": 150412, "CTTGC": 17159, "CTTGG": 81193, "CTCAA": 74053, "CTCAT": 48990, "CTCAC": 42051, "CTCAG": 422698, "CTCTA": 131837, "CTCTT": 639727, "CTCTC": 935347, "CTCTG": 31130, "CTCCA": 2007022, "CTCCT": 695693, "CTCCC": 1114817, "CTCCG": 13027, "CTCGA": 18039, "CTCGT": 21308, "CTCGC": 5822, "CTCGG": 12766, "CTGAA": 1819472, "CTGAT": 79389, "CTGAC": 20993, "CTGAG": 37444, "CTGTA": 88301, "CTGTT": 171027, "CTGTC": 1149761, "CTGTG": 56426, "CTGCA": 13923, "CTGCT": 52601, "CTGCC": 13442, "CTGCG": 4409, "CTGGA": 48066, "CTGGT": 347695, "CTGGC": 8397, "CTGGG": 218140, "CCAAA": 99021, "CCAAT": 36140, "CCAAC": 51099, "CCAAG": 20844, "CCATA": 24404, "CCATT": 51315, "CCATC": 41495, "CCATG": 13250, "CCACA": 25788, "CCACT": 45594, "CCACC": 42998, "CCACG": 12276, "CCAGA": 2261990, "CCAGT": 1394529, "CCAGC": 14194, "CCAGG": 13277, "CCTAA": 36013, "CCTAT": 33877, "CCTAC": 23384, "CCTAG": 336926, "CCTTA": 80655, "CCTTT": 435806, "CCTTC": 555689, "CCTTG": 28491, "CCTCA": 315270, "CCTCT": 653333, "CCTCC": 946841, "CCTCG": 8578, "CCTGA": 21945, "CCTGT": 1173717, "CCTGC": 10347, "CCTGG": 13426, "CCCAA": 87302, "CCCAT": 38813, "CCCAC": 48014, "CCCAG": 1673066, "CCCTA": 254101, "CCCTT": 347768, "CCCTC": 672680, "CCCTG": 17356, "CCCCA": 1049746, "CCCCT": 423327, "CCCCC": 746973, "CCCCG": 13969, "CCCGA": 17052, "CCCGT": 15057, "CCCGC": 5683, "CCCGG": 5286, "CCGAA": 20439, "CCGAT": 80124, "CCGAC": 6265, "CCGAG": 10744, "CCGTA": 10724, "CCGTT": 48683, "CCGTC": 87810, "CCGTG": 8807, "CCGCA": 6164, "CCGCT": 6945, "CCGCC": 6523, "CCGCG": 2627, "CCGGA": 12029, "CCGGT": 11040, "CCGGC": 6194, "CCGGG": 6248, "CGAAA": 718612, "CGAAT": 712848, "CGAAC": 45576, "CGAAG": 337039, "CGATA": 947943, "CGATT": 1160334, "CGATC": 162754, "CGATG": 413703, "CGACA": 7569, "CGACT": 11699, "CGACC": 7748, "CGACG": 147177, "CGAGA": 450409, "CGAGT": 541866, "CGAGC": 34400, "CGAGG": 243077, "CGTAA": 831125, "CGTAT": 1132197, "CGTAC": 81876, "CGTAG": 412281, "CGTTA": 1239274, "CGTTT": 2022530, "CGTTC": 150218, "CGTTG": 510986, "CGTCA": 23806, "CGTCT": 2203789, "CGTCC": 26781, "CGTCG": 221563, "CGTGA": 383210, "CGTGT": 693570, "CGTGC": 36973, "CGTGG": 234822, "CGCAA": 7309, "CGCAT": 8535, "CGCAC": 5752, "CGCAG": 6949, "CGCTA": 7025, "CGCTT": 16413, "CGCTC": 11731, "CGCTG": 7466, "CGCCA": 4349, "CGCCT": 9870, "CGCCC": 8482, "CGCCG": 3067, "CGCGA": 116546, "CGCGT": 160586, "CGCGC": 17930, "CGCGG": 82213, "CGGAA": 3346497, "CGGAT": 617171, "CGGAC": 65526, "CGGAG": 241104, "CGGTA": 529063, "CGGTT": 794273, "CGGTC": 71000, "CGGTG": 288325, "CGGCA": 5740, "CGGCT": 32944, "CGGCC": 6005, "CGGCG": 86566, "CGGGA": 240520, "CGGGT": 373038, "CGGGC": 30337, "CGGGG": 243764, "GAAAA": 18164340, "GAAAT": 20370375, "GAAAC": 194165, "GAAAG": 7230000, "GAATA": 14667969, "GAATT": 19928456, "GAATC": 287247, "GAATG": 7542051, "GAACA": 58323, "GAACT": 1708288, "GAACC": 40421, "GAACG": 285493, "GAAGA": 9678038, "GAAGT": 8167305, "GAAGC": 90836, "GAAGG": 3295508, "GATAA": 15048988, "GATAT": 23243666, "GATAC": 226111, "GATAG": 8384350, "GATTA": 16575225, "GATTT": 29939423, "GATTC": 497674, "GATTG": 10521165, "GATCA": 73755, "GATCT": 253456, "GATCC": 170559, "GATCG": 3748713, "GATGA": 7941075, "GATGT": 12034939, "GATGC": 98893, "GATGG": 4426924, "GACAA": 48576, "GACAT": 60859, "GACAC": 13698, "GACAG": 28934, "GACTA": 38712, "GACTT": 95828, "GACTC": 49791, "GACTG": 26479, "GACCA": 14673, "GACCT": 35560, "GACCC": 46752, "GACCG": 6268, "GACGA": 366964, "GACGT": 564372, "GACGC": 24937, "GACGG": 289666, "GAGAA": 6821402, "GAGAT": 7744719, "GAGAC": 100516, "GAGAG": 7240233, "GAGTA": 6423290, "GAGTT": 9465225, "GAGTC": 143170, "GAGTG": 4936979, "GAGCA": 2488989, "GAGCT": 36429, "GAGCC": 28148, "GAGCG": 204260, "GAGGA": 3679250, "GAGGT": 4493606, "GAGGC": 60007, "GAGGG": 2374057, "GTAAA": 17657337, "GTAAT": 18922305, "GTAAC": 193347, "GTAAG": 6932008, "GTATA": 23850997, "GTATT": 29985980, "GTATC": 437429, "GTATG": 12210607, "GTACA": 69393, "GTACT": 81371, "GTACC": 32493, "GTACG": 531330, "GTAGA": 8419377, "GTAGT": 10458504, "GTAGC": 111922, "GTAGG": 4573316, "GTTAA": 18590723, "GTTAT": 28991626, "GTTAC": 318378, "GTTAG": 9892948, "GTTTA": 27029584, "GTTTT": 48385936, "GTTTC": 799661, "GTTTG": 17375305, "GTTCA": 141905, "GTTCT": 315334, "GTTCC": 201463, "GTTCG": 872642, "GTTGA": 11309888, "GTTGT": 16449528, "GTTGC": 149129, "GTTGG": 5892605, "GTCAA": 105066, "GTCAT": 138180, "GTCAC": 2357864, "GTCAG": 50773, "GTCTA": 97249, "GTCTT": 506875, "GTCTC": 183023, "GTCTG": 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211526, "TAGAG": 7875027, "TAGTA": 16755375, "TAGTT": 22946498, "TAGTC": 327929, "TAGTG": 10714049, "TAGCA": 44949, "TAGCT": 66141, "TAGCC": 36425, "TAGCG": 398227, "TAGGA": 6576917, "TAGGT": 9697195, "TAGGC": 99275, "TAGGG": 4977355, "TTAAA": 42034865, "TTAAT": 44061521, "TTAAC": 428002, "TTAAG": 15270685, "TTATA": 51896629, "TTATT": 72401795, "TTATC": 993671, "TTATG": 24599337, "TTACA": 151868, "TTACT": 212139, "TTACC": 76686, "TTACG": 1112347, "TTAGA": 17617435, "TTAGT": 22528113, "TTAGC": 240553, "TTAGG": 9457304, "TTTAA": 51939186, "TTTAT": 76598397, "TTTAC": 803901, "TTTAG": 24988561, "TTTTA": 82067896, "TTTTT": 152319211, "TTTTC": 2924061, "TTTTG": 48676557, "TTTCA": 586851, "TTTCT": 1368921, "TTTCC": 1029011, "TTTCG": 2280474, "TTTGA": 30768090, "TTTGT": 45438685, "TTTGC": 403609, "TTTGG": 16622085, "TTCAA": 378880, "TTCAT": 369531, "TTCAC": 76937, "TTCAG": 144478, "TTCTA": 291465, "TTCTT": 1127788, "TTCTC": 976020, "TTCTG": 198673, "TTCCA": 54176, "TTCCT": 794953, "TTCCC": 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"CGTTT": 1751023, "CGTTC": 131387, "CGTTG": 463310, "CGTCA": 14249, "CGTCT": 447104, "CGTCC": 16131, "CGTCG": 198430, "CGTGA": 349223, "CGTGT": 624817, "CGTGC": 32777, "CGTGG": 213854, "CGCAA": 5025, "CGCAT": 6259, "CGCAC": 2618, "CGCAG": 3130, "CGCTA": 4663, "CGCTT": 10772, "CGCTC": 5864, "CGCTG": 3366, "CGCCA": 2102, "CGCCT": 5302, "CGCCC": 5462, "CGCCG": 1809, "CGCGA": 106541, "CGCGT": 146276, "CGCGC": 15543, "CGCGG": 74833, "CGGAA": 402378, "CGGAT": 564357, "CGGAC": 58122, "CGGAG": 213345, "CGGTA": 485017, "CGGTT": 709720, "CGGTC": 62730, "CGGTG": 263511, "CGGCA": 2810, "CGGCT": 17366, "CGGCC": 4185, "CGGCG": 78397, "CGGGA": 217907, "CGGGT": 335708, "CGGGC": 27132, "CGGGG": 217232, "GAAAA": 16421952, "GAAAT": 18805197, "GAAAC": 172154, "GAAAG": 6529854, "GAATA": 13511188, "GAATT": 18368343, "GAATC": 260629, "GAATG": 6930284, "GAACA": 45929, "GAACT": 393867, "GAACC": 28156, "GAACG": 260506, "GAAGA": 6373770, "GAAGT": 7496526, "GAAGC": 80508, "GAAGG": 2940653, "GATAA": 13841688, 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3393198, "GGATA": 7172979, "GGATT": 9468037, "GGATC": 164277, "GGATG": 3963069, "GGACA": 23377, "GGACT": 34642, "GGACC": 17102, "GGACG": 219249, "GGAGA": 3402207, "GGAGT": 3730193, "GGAGC": 43300, "GGAGG": 2126157, "GGTAA": 6993615, "GGTAT": 10318519, "GGTAC": 113957, "GGTAG": 3971848, "GGTTA": 10575576, "GGTTT": 15318648, "GGTTC": 295826, "GGTTG": 5626537, "GGTCA": 53019, "GGTCT": 101560, "GGTCC": 82533, "GGTCG": 359192, "GGTGA": 4413407, "GGTGT": 6452433, "GGTGC": 54748, "GGTGG": 3275608, "GGCAA": 20897, "GGCAT": 33254, "GGCAC": 4057, "GGCAG": 16969, "GGCTA": 20594, "GGCTT": 61904, "GGCTC": 31120, "GGCTG": 14521, "GGCCA": 5889, "GGCCT": 28515, "GGCCC": 33016, "GGCCG": 9065, "GGCGA": 194167, "GGCGT": 324532, "GGCGC": 21571, "GGCGG": 190519, "GGGAA": 3433996, "GGGAT": 4349405, "GGGAC": 62817, "GGGAG": 1942536, "GGGTA": 4150992, "GGGTT": 7100517, "GGGTC": 145860, "GGGTG": 2837635, "GGGCA": 19727, "GGGCT": 27725, "GGGCC": 22195, "GGGCG": 210352, "GGGGA": 2317110, "GGGGT": 3847073, 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"GGGGG": 1123317 }, "overrepresented_sequences": {} }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/Qui5 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/Qui5 (paired)_R2.fastq.gz -o output/13-wgbs/trimmed/Qui5_R1.fq.gz -O output/13-wgbs/trimmed/Qui5_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs/fastp/Qui5.fastp.json --html output/13-wgbs/fastp/Qui5.fastp.html " }, "RP1 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 84335388, "total_bases": 12734643588, "q20_bases": 12585490360, "q30_bases": 12188219019, "q20_rate": 0.988288, "q30_rate": 0.957091, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.210136 }, "after_filtering": { "total_reads": 83039660, "total_bases": 11528495410, "q20_bases": 11428900502, "q30_bases": 11108538555, "q20_rate": 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16134, "TGGCT": 15615, "TGGCC": 12228, "TGGCG": 7611, "TGGGA": 108449, "TGGGT": 21197, "TGGGC": 14011, "TGGGG": 169384, "CAAAA": 61418957, "CAAAT": 36239357, "CAAAC": 20261290, "CAAAG": 189736, "CAATA": 37200830, "CAATT": 23887240, "CAATC": 12435423, "CAATG": 88604, "CAACA": 20115225, "CAACT": 11752988, "CAACC": 7344698, "CAACG": 615447, "CAAGA": 218951, "CAAGT": 50046, "CAAGC": 31126, "CAAGG": 21085, "CATAA": 31482713, "CATAT": 29130011, "CATAC": 14425387, "CATAG": 167905, "CATTA": 24791344, "CATTT": 24243551, "CATTC": 9311266, "CATTG": 55308, "CATCA": 14651788, "CATCT": 9065456, "CATCC": 5081414, "CATCG": 513710, "CATGA": 83760, "CATGT": 127896, "CATGC": 34799, "CATGG": 16142, "CACAA": 20243661, "CACAT": 14000561, "CACAC": 11539739, "CACAG": 81432, "CACTA": 14166025, "CACTT": 9229156, "CACTC": 6344602, "CACTG": 43063, "CACCA": 8604316, "CACCT": 5800283, "CACCC": 3988926, "CACCG": 367178, "CACGA": 820320, "CACGT": 505878, "CACGC": 271349, "CACGG": 6430, "CAGAA": 85032, "CAGAT": 471836, "CAGAC": 55384, "CAGAG": 31636, "CAGTA": 43869, "CAGTT": 39324, "CAGTC": 27261, "CAGTG": 30851, "CAGCA": 35458, "CAGCT": 27126, "CAGCC": 28664, "CAGCG": 6163, "CAGGA": 28067, "CAGGT": 13749, "CAGGC": 15053, "CAGGG": 22514, "CTAAA": 31616285, "CTAAT": 20292909, "CTAAC": 11555094, "CTAAG": 144601, "CTATA": 27435063, "CTATT": 17562261, "CTATC": 10331655, "CTATG": 59055, "CTACA": 13944947, "CTACT": 8460975, "CTACC": 5121176, "CTACG": 494595, "CTAGA": 271182, "CTAGT": 30200, "CTAGC": 16824, "CTAGG": 23434, "CTTAA": 19629608, "CTTAT": 15024440, "CTTAC": 8358268, "CTTAG": 108827, "CTTTA": 18940471, "CTTTT": 16274727, "CTTTC": 8969181, "CTTTG": 111517, "CTTCA": 10233133, "CTTCT": 7128589, "CTTCC": 4651746, "CTTCG": 420170, "CTTGA": 55649, "CTTGT": 38105, "CTTGC": 21313, "CTTGG": 999060, "CTCAA": 11937485, "CTCAT": 7463256, "CTCAC": 5307055, "CTCAG": 81571, "CTCTA": 10571254, "CTCTT": 6836312, "CTCTC": 9743294, "CTCTG": 50783, "CTCCA": 5368279, "CTCCT": 3896575, "CTCCC": 2926461, 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22577, "GTTCA": 354312, "GTTCT": 239366, "GTTCC": 154094, "GTTCG": 48481, "GTTGA": 16049, "GTTGT": 31949, "GTTGC": 23459, "GTTGG": 22454, "GTCAA": 603924, "GTCAT": 461974, "GTCAC": 292452, "GTCAG": 15436, "GTCTA": 365682, "GTCTT": 1343193, "GTCTC": 202269, "GTCTG": 14652, "GTCCA": 239657, "GTCCT": 189055, "GTCCC": 137909, "GTCCG": 81598, "GTCGA": 158209, "GTCGT": 1899792, "GTCGC": 713917, "GTCGG": 31633, "GTGAA": 40929, "GTGAT": 17971, "GTGAC": 11128, "GTGAG": 50481, "GTGTA": 2367072, "GTGTT": 36471, "GTGTC": 1232920, "GTGTG": 126700, "GTGCA": 15144, "GTGCT": 124340, "GTGCC": 11365, "GTGCG": 5476, "GTGGA": 78206, "GTGGT": 758406, "GTGGC": 15613, "GTGGG": 119916, "GCAAA": 794141, "GCAAT": 467148, "GCAAC": 275454, "GCAAG": 19829, "GCATA": 446175, "GCATT": 315581, "GCATC": 185972, "GCATG": 12423, "GCACA": 267579, "GCACT": 193476, "GCACC": 124443, "GCACG": 50985, "GCAGA": 34660, "GCAGT": 17873, "GCAGC": 12209, "GCAGG": 22192, "GCTAA": 511335, "GCTAT": 447539, "GCTAC": 221570, "GCTAG": 8923, "GCTTA": 307667, "GCTTT": 302633, "GCTTC": 171500, "GCTTG": 10839, "GCTCA": 245858, "GCTCT": 324073, "GCTCC": 115001, "GCTCG": 54721, "GCTGA": 15181, "GCTGT": 11449, "GCTGC": 10200, "GCTGG": 15970, "GCCAA": 379107, "GCCAT": 320068, "GCCAC": 168373, "GCCAG": 15082, "GCCTA": 218451, "GCCTT": 167550, "GCCTC": 113929, "GCCTG": 14963, "GCCCA": 188933, "GCCCT": 149699, "GCCCC": 142810, "GCCCG": 48372, "GCCGA": 81244, "GCCGT": 672395, "GCCGC": 30973, "GCCGG": 5544, "GCGAA": 152214, "GCGAT": 96586, "GCGAC": 59762, "GCGAG": 14435, "GCGTA": 85123, "GCGTT": 52721, "GCGTC": 1946415, "GCGTG": 12815, "GCGCA": 46165, "GCGCT": 35938, "GCGCC": 36639, "GCGCG": 29356, "GCGGA": 36365, "GCGGT": 5125, "GCGGC": 5738, "GCGGG": 19384, "GGAAA": 10523426, "GGAAT": 3258237, "GGAAC": 2539712, "GGAAG": 10802725, "GGATA": 3020863, "GGATT": 2019185, "GGATC": 1697251, "GGATG": 73956, "GGACA": 1466773, "GGACT": 918723, "GGACC": 756621, "GGACG": 89706, "GGAGA": 14431524, "GGAGT": 771972, "GGAGC": 660279, "GGAGG": 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456684, "AGAGA": 13761704, "AGAGT": 8944799, "AGAGC": 221862, "AGAGG": 4467098, "AGTAA": 15675270, "AGTAT": 21544971, "AGTAC": 217045, "AGTAG": 8235253, "AGTTA": 19073338, "AGTTT": 30968228, "AGTTC": 431476, "AGTTG": 11239852, "AGTCA": 318002, "AGTCT": 136395, "AGTCC": 88101, "AGTCG": 539008, "AGTGA": 10150164, "AGTGT": 13556795, "AGTGC": 115651, "AGTGG": 4638666, "AGCAA": 54886, "AGCAT": 63036, "AGCAC": 263767, "AGCAG": 35007, "AGCTA": 44129, "AGCTT": 76889, "AGCTC": 41002, "AGCTG": 30287, "AGCCA": 16060, "AGCCT": 40501, "AGCCC": 40487, "AGCCG": 4754, "AGCGA": 416988, "AGCGT": 475769, "AGCGC": 32582, "AGCGG": 232615, "AGGAA": 7994625, "AGGAT": 7647176, "AGGAC": 96046, "AGGAG": 3902119, "AGGTA": 8613743, "AGGTT": 12050515, "AGGTC": 182113, "AGGTG": 5528891, "AGGCA": 32978, "AGGCT": 40582, "AGGCC": 23288, "AGGCG": 202956, "AGGGA": 4012244, "AGGGT": 5191457, "AGGGC": 77070, "AGGGG": 3305116, "TAAAA": 47143407, "TAAAT": 53417730, "TAAAC": 475926, "TAAAG": 18816825, "TAATA": 45297280, 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51346327, "TTGTC": 664629, "TTGTG": 19241076, "TTGCA": 111478, "TTGCT": 173626, "TTGCC": 57223, "TTGCG": 622009, "TTGGA": 12871127, "TTGGT": 17708604, "TTGGC": 179156, "TTGGG": 7782873, "TCAAA": 353866, "TCAAT": 272846, "TCAAC": 82377, "TCAAG": 111284, "TCATA": 232002, "TCATT": 392035, "TCATC": 59408, "TCATG": 139415, "TCACA": 33768, "TCACT": 321385, "TCACC": 45151, "TCACG": 19195, "TCAGA": 108847, "TCAGT": 143643, "TCAGC": 19010, "TCAGG": 67608, "TCTAA": 202161, "TCTAT": 312324, "TCTAC": 35941, "TCTAG": 138909, "TCTTA": 247871, "TCTTT": 1092680, "TCTTC": 793037, "TCTTG": 182799, "TCTCA": 67729, "TCTCT": 758254, "TCTCC": 1023922, "TCTCG": 30196, "TCTGA": 353519, "TCTGT": 198741, "TCTGC": 49814, "TCTGG": 278021, "TCCAA": 49216, "TCCAT": 48536, "TCCAC": 28503, "TCCAG": 263531, "TCCTA": 45479, "TCCTT": 535184, "TCCTC": 907214, "TCCTG": 37318, "TCCCA": 82662, "TCCCT": 579166, "TCCCC": 1006249, "TCCCG": 10944, "TCCGA": 21527, "TCCGT": 26520, "TCCGC": 7730, "TCCGG": 11226, "TCGAA": 1124520, "TCGAT": 1623829, "TCGAC": 84493, "TCGAG": 816849, "TCGTA": 1425687, "TCGTT": 2244064, "TCGTC": 184703, "TCGTG": 1059464, "TCGCA": 10163, "TCGCT": 14711, "TCGCC": 7084, "TCGCG": 228778, "TCGGA": 818259, "TCGGT": 1049193, "TCGGC": 60693, "TCGGG": 562722, "TGAAA": 26366720, "TGAAT": 23752313, "TGAAC": 465164, "TGAAG": 9934082, "TGATA": 26008833, "TGATT": 32187914, "TGATC": 434740, "TGATG": 14120630, "TGACA": 75612, "TGACT": 104435, "TGACC": 44793, "TGACG": 583381, "TGAGA": 8983485, "TGAGT": 10164628, "TGAGC": 130535, "TGAGG": 4593960, "TGTAA": 26240661, "TGTAT": 41061790, "TGTAC": 378604, "TGTAG": 13389064, "TGTTA": 32357949, "TGTTT": 52490562, "TGTTC": 676931, "TGTTG": 18948487, "TGTCA": 160290, "TGTCT": 276581, "TGTCC": 141893, "TGTCG": 1082879, "TGTGA": 13371338, "TGTGT": 22863215, "TGTGC": 184114, "TGTGG": 7131555, "TGCAA": 82206, "TGCAT": 106240, "TGCAC": 15307, "TGCAG": 49759, "TGCTA": 74731, "TGCTT": 181883, "TGCTC": 54470, "TGCTG": 52602, "TGCCA": 22258, "TGCCT": 51482, "TGCCC": 52320, "TGCCG": 47748, "TGCGA": 445855, "TGCGT": 581023, "TGCGC": 38060, "TGCGG": 243340, "TGGAA": 10457598, "TGGAT": 12132512, "TGGAC": 134316, "TGGAG": 5190632, "TGGTA": 12319049, "TGGTT": 16875166, "TGGTC": 265322, "TGGTG": 7954551, "TGGCA": 43637, "TGGCT": 62170, "TGGCC": 33110, "TGGCG": 348469, "TGGGA": 5298436, "TGGGT": 6750665, "TGGGC": 91372, "TGGGG": 4115717, "CAAAA": 278994, "CAAAT": 217467, "CAAAC": 75622, "CAAAG": 92036, "CAATA": 157618, "CAATT": 228035, "CAATC": 32718, "CAATG": 89816, "CAACA": 34630, "CAACT": 67965, "CAACC": 42330, "CAACG": 14531, "CAAGA": 67926, "CAAGT": 88513, "CAAGC": 13542, "CAAGG": 44850, "CATAA": 150203, "CATAT": 233470, "CATAC": 21269, "CATAG": 79263, "CATTA": 175160, "CATTT": 375016, "CATTC": 59891, "CATTG": 120151, "CATCA": 36811, "CATCT": 52167, "CATCC": 29335, "CATCG": 12996, "CATGA": 83093, "CATGT": 135283, "CATGC": 13842, "CATGG": 53665, "CACAA": 32222, "CACAT": 32289, "CACAC": 275009, "CACAG": 19344, "CACTA": 21736, "CACTT": 79254, "CACTC": 37551, "CACTG": 263234, "CACCA": 39739, "CACCT": 25877, "CACCC": 36642, "CACCG": 6419, "CACGA": 11973, "CACGT": 260958, "CACGC": 6221, "CACGG": 7855, "CAGAA": 87362, "CAGAT": 95689, "CAGAC": 16430, "CAGAG": 49230, "CAGTA": 77631, "CAGTT": 130583, "CAGTC": 258771, "CAGTG": 60578, "CAGCA": 18571, "CAGCT": 18997, "CAGCC": 17039, "CAGCG": 5650, "CAGGA": 44244, "CAGGT": 56469, "CAGGC": 15141, "CAGGG": 34315, "CTAAA": 167915, "CTAAT": 142710, "CTAAC": 26931, "CTAAG": 52195, "CTATA": 181336, "CTATT": 227020, "CTATC": 34514, "CTATG": 88858, "CTACA": 23847, "CTACT": 35472, "CTACC": 18376, "CTACG": 8548, "CTAGA": 68687, "CTAGT": 96263, "CTAGC": 9507, "CTAGG": 48958, "CTTAA": 164430, "CTTAT": 189792, "CTTAC": 30936, "CTTAG": 69559, "CTTTA": 249516, "CTTTT": 1015504, "CTTTC": 453697, "CTTTG": 174909, "CTTCA": 87558, "CTTCT": 550416, "CTTCC": 638964, "CTTCG": 21298, "CTTGA": 105745, "CTTGT": 150004, "CTTGC": 19834, "CTTGG": 63781, "CTCAA": 72621, "CTCAT": 44887, "CTCAC": 39270, "CTCAG": 33408, "CTCTA": 47677, "CTCTT": 469744, "CTCTC": 754408, "CTCTG": 33796, "CTCCA": 272473, "CTCCT": 556765, "CTCCC": 857768, "CTCCG": 8379, "CTCGA": 16050, "CTCGT": 20846, "CTCGC": 6036, "CTCGG": 10165, "CTGAA": 330911, "CTGAT": 81671, "CTGAC": 17994, "CTGAG": 40974, "CTGTA": 99873, "CTGTT": 158540, "CTGTC": 271845, "CTGTG": 65090, "CTGCA": 19563, "CTGCT": 49042, "CTGCC": 17105, "CTGCG": 5264, "CTGGA": 47152, "CTGGT": 163604, "CTGGC": 13192, "CTGGG": 142897, "CCAAA": 86139, "CCAAT": 31633, "CCAAC": 41017, "CCAAG": 22062, "CCATA": 22422, "CCATT": 54010, "CCATC": 31997, "CCATG": 18536, "CCACA": 24184, "CCACT": 42312, "CCACC": 35510, "CCACG": 8066, "CCAGA": 35827, "CCAGT": 257706, "CCAGC": 17200, "CCAGG": 19868, "CCTAA": 31111, "CCTAT": 28917, "CCTAC": 18769, "CCTAG": 14157, "CCTTA": 33183, "CCTTT": 357766, "CCTTC": 442579, "CCTTG": 28446, "CCTCA": 52229, "CCTCT": 518737, "CCTCC": 736002, "CCTCG": 7442, "CCTGA": 23487, "CCTGT": 28091, "CCTGC": 14870, "CCTGG": 20712, "CCCAA": 61551, "CCCAT": 31102, "CCCAC": 38299, "CCCAG": 40690, "CCCTA": 23045, "CCCTT": 274543, "CCCTC": 521614, "CCCTG": 19222, "CCCCA": 63192, "CCCCT": 317532, "CCCCC": 535030, "CCCCG": 8305, "CCCGA": 8698, "CCCGT": 9811, "CCCGC": 5102, "CCCGG": 6275, "CCGAA": 14509, "CCGAT": 15648, "CCGAC": 5107, "CCGAG": 7689, "CCGTA": 9121, "CCGTT": 31850, "CCGTC": 46220, "CCGTG": 9150, "CCGCA": 4067, "CCGCT": 5549, "CCGCC": 6034, "CCGCG": 3041, "CCGGA": 7516, "CCGGT": 9237, "CCGGC": 5434, "CCGGG": 7457, "CGAAA": 909136, "CGAAT": 888932, "CGAAC": 48331, "CGAAG": 412420, "CGATA": 1186273, "CGATT": 1399306, "CGATC": 113249, "CGATG": 504243, "CGACA": 7925, "CGACT": 10795, "CGACC": 6261, "CGACG": 178197, "CGAGA": 554657, "CGAGT": 654916, "CGAGC": 40862, "CGAGG": 291431, "CGTAA": 1032350, "CGTAT": 1378099, "CGTAC": 99722, "CGTAG": 490269, "CGTTA": 1489027, "CGTTT": 2181454, "CGTTC": 161159, "CGTTG": 595543, "CGTCA": 18397, "CGTCT": 367937, "CGTCC": 17143, "CGTCG": 255250, "CGTGA": 698178, "CGTGT": 802024, "CGTGC": 42715, "CGTGG": 275483, "CGCAA": 7752, "CGCAT": 9221, "CGCAC": 4678, "CGCAG": 5305, "CGCTA": 7131, "CGCTT": 15116, "CGCTC": 8022, "CGCTG": 5654, "CGCCA": 4617, "CGCCT": 7853, "CGCCC": 7646, "CGCCG": 3215, "CGCGA": 142926, "CGCGT": 195810, "CGCGC": 22649, "CGCGG": 101283, "CGGAA": 543725, "CGGAT": 753575, "CGGAC": 81722, "CGGAG": 290265, "CGGTA": 645815, "CGGTT": 890002, "CGGTC": 79327, "CGGTG": 338829, "CGGCA": 5044, "CGGCT": 15206, "CGGCC": 5890, "CGGCG": 103572, "CGGGA": 290526, "CGGGT": 424273, "CGGGC": 37271, "CGGGG": 278655, "GAAAA": 22473922, "GAAAT": 24838618, "GAAAC": 242106, "GAAAG": 8790824, "GAATA": 17863715, "GAATT": 23330373, "GAATC": 550272, "GAATG": 9008384, "GAACA": 63658, "GAACT": 323583, "GAACC": 32841, "GAACG": 356106, "GAAGA": 8462522, "GAAGT": 9647689, "GAAGC": 107911, "GAAGG": 3786738, "GATAA": 18367088, "GATAT": 27280158, "GATAC": 270682, "GATAG": 10120195, "GATTA": 19301124, "GATTT": 33788997, "GATTC": 528904, "GATTG": 11918932, "GATCA": 77830, "GATCT": 184199, "GATCC": 137763, "GATCG": 653250, "GATGA": 9472312, "GATGT": 13744502, "GATGC": 119874, "GATGG": 5068133, "GACAA": 57178, "GACAT": 68288, "GACAC": 11890, "GACAG": 36670, "GACTA": 44747, "GACTT": 98230, "GACTC": 42545, "GACTG": 31543, "GACCA": 16922, "GACCT": 32695, "GACCC": 40280, "GACCG": 4562, "GACGA": 461894, "GACGT": 671723, "GACGC": 27143, "GACGG": 354427, "GAGAA": 8355472, "GAGAT": 9227956, "GAGAC": 118684, "GAGAG": 10176776, "GAGTA": 7538064, "GAGTT": 10662185, "GAGTC": 151659, "GAGTG": 6042255, "GAGCA": 142170, "GAGCT": 41230, "GAGCC": 23262, "GAGCG": 249702, "GAGGA": 4405051, "GAGGT": 5034263, "GAGGC": 67779, "GAGGG": 2567338, "GTAAA": 21288476, "GTAAT": 22194180, "GTAAC": 232112, "GTAAG": 8157298, "GTATA": 28367920, "GTATT": 34027892, "GTATC": 481183, "GTATG": 13900606, "GTACA": 77102, "GTACT": 84976, "GTACC": 31408, "GTACG": 647401, "GTAGA": 9874341, "GTAGT": 11892932, "GTAGC": 127514, "GTAGG": 5169787, "GTTAA": 21735822, "GTTAT": 32721524, "GTTAC": 364357, "GTTAG": 11067199, "GTTTA": 30703789, "GTTTT": 53389362, "GTTTC": 822619, "GTTTG": 19038885, "GTTCA": 149803, "GTTCT": 286058, "GTTCC": 155759, "GTTCG": 1008737, "GTTGA": 12810859, "GTTGT": 18014770, "GTTGC": 168221, "GTTGG": 6451238, "GTCAA": 111364, "GTCAT": 136892, "GTCAC": 258929, "GTCAG": 53310, "GTCTA": 100220, "GTCTT": 327624, "GTCTC": 144286, "GTCTG": 301164, "GTCCA": 16296, "GTCCT": 129552, "GTCCC": 151877, "GTCCG": 8609, "GTCGA": 641317, "GTCGT": 1154120, "GTCGC": 48519, "GTCGG": 483310, "GTGAA": 10716690, "GTGAT": 13808907, "GTGAC": 180615, "GTGAG": 5005806, "GTGTA": 15215394, "GTGTT": 18692089, "GTGTC": 314944, "GTGTG": 10783715, "GTGCA": 45218, "GTGCT": 58846, "GTGCC": 48642, "GTGCG": 262761, "GTGGA": 5244979, "GTGGT": 7283519, "GTGGC": 123911, "GTGGG": 3347633, "GCAAA": 68107, "GCAAT": 65378, "GCAAC": 12667, "GCAAG": 29153, "GCATA": 66262, "GCATT": 94571, "GCATC": 15016, "GCATG": 42349, "GCACA": 262614, "GCACT": 14485, "GCACC": 11121, "GCACG": 5355, "GCAGA": 36053, "GCAGT": 44101, 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11750, "CCGAA": 1059410, "CCGAT": 735765, "CCGAC": 427541, "CCGAG": 8094, "CCGTA": 1248581, "CCGTT": 363475, "CCGTC": 319599, "CCGTG": 14744, "CCGCA": 217744, "CCGCT": 213171, "CCGCC": 195710, "CCGCG": 83770, "CCGGA": 8362, "CCGGT": 8091, "CCGGC": 2390, "CCGGG": 6164, "CGAAA": 2463167, "CGAAT": 1366866, "CGAAC": 877376, "CGAAG": 29227, "CGATA": 1445303, "CGATT": 917444, "CGATC": 795847, "CGATG": 5461, "CGACA": 662131, "CGACT": 475001, "CGACC": 391110, "CGACG": 215143, "CGAGA": 18105, "CGAGT": 6663, "CGAGC": 5643, "CGAGG": 9891, "CGTAA": 1259459, "CGTAT": 1647692, "CGTAC": 556740, "CGTAG": 286035, "CGTTA": 765694, "CGTTT": 679379, "CGTTC": 306963, "CGTTG": 12782, "CGTCA": 518522, "CGTCT": 411082, "CGTCC": 245638, "CGTCG": 2169485, "CGTGA": 9193, "CGTGT": 1908874, "CGTGC": 4999, "CGTGG": 50003, "CGCAA": 540180, "CGCAT": 360745, "CGCAC": 231016, "CGCAG": 8851, "CGCTA": 454008, "CGCTT": 329216, "CGCTC": 208839, "CGCTG": 2251, "CGCCA": 344059, "CGCCT": 179836, "CGCCC": 174089, "CGCCG": 773294, "CGCGA": 189681, "CGCGT": 109527, "CGCGC": 83824, "CGCGG": 3387, "CGGAA": 2653621, "CGGAT": 5576, "CGGAC": 5530, "CGGAG": 20234, "CGGTA": 6127, "CGGTT": 12407, "CGGTC": 3592, "CGGTG": 841633, "CGGCA": 4723, "CGGCT": 2305, "CGGCC": 3154, "CGGCG": 3630, "CGGGA": 37033, "CGGGT": 4441, "CGGGC": 2625, "CGGGG": 19725, "GAAAA": 7722331, "GAAAT": 3294744, "GAAAC": 1708021, "GAAAG": 4931525, "GAATA": 3067990, "GAATT": 2092739, "GAATC": 1320616, "GAATG": 35626, "GAACA": 1613103, "GAACT": 928783, "GAACC": 627014, "GAACG": 183556, "GAAGA": 9237807, "GAAGT": 320324, "GAAGC": 284191, "GAAGG": 4770913, "GATAA": 2838955, "GATAT": 2007152, "GATAC": 1147688, "GATAG": 46195, "GATTA": 1821620, "GATTT": 1489282, "GATTC": 797392, "GATTG": 10988, "GATCA": 1338386, "GATCT": 1736913, "GATCC": 485066, "GATCG": 2941011, "GATGA": 38776, "GATGT": 10874, "GATGC": 5099, "GATGG": 38663, "GACAA": 1503283, "GACAT": 901977, "GACAC": 522644, "GACAG": 14067, "GACTA": 903328, "GACTT": 582491, "GACTC": 347610, 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output/13-wgbs/fastp/Rio2.fastp.html " }, "RP3 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 66760720, "total_bases": 10080868720, "q20_bases": 9947606339, "q30_bases": 9610873513, "q20_rate": 0.986781, "q30_rate": 0.953378, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.216155 }, "after_filtering": { "total_reads": 65546394, "total_bases": 9034155486, "q20_bases": 8952579332, "q30_bases": 8697272677, "q20_rate": 0.99097, "q30_rate": 0.96271, "read1_mean_length": 137, "read2_mean_length": 137, "gc_content": 0.189349 } }, "filtering_result": { "passed_filter_reads": 65546394, "low_quality_reads": 506754, "too_many_N_reads": 1136, "too_short_reads": 706436, "too_long_reads": 0 }, "duplication": { "rate": 0.0391718 }, "insert_size": { "peak": 184, "unknown": 663253, "histogram": [ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 2302, 2, 0, 0, 0, 0, 0, 0, 0, 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232783, "TTATA": 38245070, "TTATT": 32080430, "TTATC": 14701363, "TTATG": 91693, "TTACA": 21207434, "TTACT": 12863248, "TTACC": 7115281, "TTACG": 825133, "TTAGA": 514895, "TTAGT": 52464, "TTAGC": 19817, "TTAGG": 14927, "TTTAA": 43093432, "TTTAT": 34925786, "TTTAC": 17101695, "TTTAG": 243048, "TTTTA": 37653017, "TTTTT": 36096400, "TTTTC": 18182149, "TTTTG": 93279, "TTTCA": 21158532, "TTTCT": 15422399, "TTTCC": 8087080, "TTTCG": 735535, "TTTGA": 105143, "TTTGT": 77536, "TTTGC": 30468, "TTTGG": 17844, "TTCAA": 22946217, "TTCAT": 17172159, "TTCAC": 8626396, "TTCAG": 92273, "TTCTA": 15248789, "TTCTT": 12809542, "TTCTC": 6875347, "TTCTG": 41129, "TTCCA": 8427317, "TTCCT": 6421509, "TTCCC": 3662092, "TTCCG": 535952, "TTCGA": 896293, "TTCGT": 619333, "TTCGC": 300315, "TTCGG": 11165, "TTGAA": 119447, "TTGAT": 88415, "TTGAC": 42399, "TTGAG": 8628, "TTGTA": 66702, "TTGTT": 90919, "TTGTC": 81499, "TTGTG": 19327, "TTGCA": 32618, "TTGCT": 25593, "TTGCC": 118435, "TTGCG": 4347, "TTGGA": 14065, 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388320, "TCGTG": 2151216, "TCGCA": 372417, "TCGCT": 344616, "TCGCC": 838849, "TCGCG": 115021, "TCGGA": 3037548, "TCGGT": 677241, "TCGGC": 5282, "TCGGG": 36961, "TGAAA": 209259, "TGAAT": 109754, "TGAAC": 57821, "TGAAG": 26991, "TGATA": 121799, "TGATT": 90579, "TGATC": 43419, "TGATG": 1180409, "TGACA": 66382, "TGACT": 36182, "TGACC": 32295, "TGACG": 11801, "TGAGA": 32202, "TGAGT": 8250, "TGAGC": 3382, "TGAGG": 48044, "TGTAA": 176838, "TGTAT": 95135, "TGTAC": 41159, "TGTAG": 3450640, "TGTTA": 81020, "TGTTT": 110871, "TGTTC": 49052, "TGTTG": 36222, "TGTCA": 113348, "TGTCT": 46454, "TGTCC": 32084, "TGTCG": 468753, "TGTGA": 1244259, "TGTGT": 1335075, "TGTGC": 74889, "TGTGG": 140268, "TGCAA": 47458, "TGCAT": 39489, "TGCAC": 22164, "TGCAG": 6621, "TGCTA": 29295, "TGCTT": 28538, "TGCTC": 109456, "TGCTG": 4296, "TGCCA": 49933, "TGCCT": 15231, "TGCCC": 18280, "TGCCG": 70644, "TGCGA": 4788, "TGCGT": 4787, "TGCGC": 3459, "TGCGG": 3086, "TGGAA": 43537, "TGGAT": 11205, "TGGAC": 4726, "TGGAG": 75903, 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4247325, "CTCCT": 3167148, "CTCCC": 2365792, "CTCCG": 322688, "CTCGA": 646668, "CTCGT": 349972, "CTCGC": 236736, "CTCGG": 681897, "CTGAA": 68489, "CTGAT": 43809, "CTGAC": 23835, "CTGAG": 17154, "CTGTA": 49631, "CTGTT": 48215, "CTGTC": 23808, "CTGTG": 75812, "CTGCA": 21459, "CTGCT": 15042, "CTGCC": 10582, "CTGCG": 2682, "CTGGA": 22579, "CTGGT": 2833, "CTGGC": 1876, "CTGGG": 49667, "CCAAA": 16412159, "CCAAT": 9715072, "CCAAC": 5588832, "CCAAG": 68789, "CCATA": 10379906, "CCATT": 7683800, "CCATC": 4203159, "CCATG": 33001, "CCACA": 6072717, "CCACT": 3847198, "CCACC": 3558588, "CCACG": 230558, "CCAGA": 161511, "CCAGT": 21218, "CCAGC": 12524, "CCAGG": 5108, "CCTAA": 9051553, "CCTAT": 7843889, "CCTAC": 4179358, "CCTAG": 76632, "CCTTA": 6642449, "CCTTT": 6478852, "CCTTC": 3240019, "CCTTG": 19554, "CCTCA": 3796323, "CCTCT": 3636953, "CCTCC": 2349767, "CCTCG": 284976, "CCTGA": 27293, "CCTGT": 23146, "CCTGC": 11045, "CCTGG": 3606, "CCCAA": 6468222, "CCCAT": 3940273, "CCCAC": 3118968, "CCCAG": 69480, "CCCTA": 4724247, "CCCTT": 3198988, "CCCTC": 2422278, "CCCTG": 20598, "CCCCA": 3597477, "CCCCT": 2744072, "CCCCC": 5928255, "CCCCG": 235815, "CCCGA": 442565, "CCCGT": 224626, "CCCGC": 164809, "CCCGG": 20908, "CCGAA": 994488, "CCGAT": 707942, "CCGAC": 397779, "CCGAG": 8580, "CCGTA": 952858, "CCGTT": 373207, "CCGTC": 357415, "CCGTG": 71114, "CCGCA": 204826, "CCGCT": 194339, "CCGCC": 181617, "CCGCG": 80948, "CCGGA": 8985, "CCGGT": 15811, "CCGGC": 2472, "CCGGG": 8830, "CGAAA": 2278237, "CGAAT": 1280440, "CGAAC": 807306, "CGAAG": 29953, "CGATA": 1358969, "CGATT": 884451, "CGATC": 905123, "CGATG": 6500, "CGACA": 622866, "CGACT": 442646, "CGACC": 395901, "CGACG": 205584, "CGAGA": 18370, "CGAGT": 7271, "CGAGC": 5990, "CGAGG": 9778, "CGTAA": 1180785, "CGTAT": 1331785, "CGTAC": 540043, "CGTAG": 21695, "CGTTA": 741356, "CGTTT": 663712, "CGTTC": 296068, "CGTTG": 29034, "CGTCA": 482079, "CGTCT": 441417, "CGTCC": 242169, "CGTCG": 2360533, "CGTGA": 9897, "CGTGT": 2097116, "CGTGC": 5534, "CGTGG": 107049, "CGCAA": 509683, "CGCAT": 344804, "CGCAC": 216925, "CGCAG": 7382, "CGCTA": 430049, "CGCTT": 309419, "CGCTC": 203076, "CGCTG": 2383, "CGCCA": 460929, "CGCCT": 168017, "CGCCC": 173377, "CGCCG": 485929, "CGCGA": 182326, "CGCGT": 107655, "CGCGC": 80824, "CGCGG": 3412, "CGGAA": 3007382, "CGGAT": 6077, "CGGAC": 5408, "CGGAG": 19875, "CGGTA": 6870, "CGGTT": 29871, "CGGTC": 4557, "CGGTG": 650635, "CGGCA": 4794, "CGGCT": 2563, "CGGCC": 3280, "CGGCG": 3811, "CGGGA": 36678, "CGGGT": 5518, "CGGGC": 2757, "CGGGG": 24030, "GAAAA": 6514635, "GAAAT": 2858598, "GAAAC": 1477337, "GAAAG": 4675486, "GAATA": 2553823, "GAATT": 1831151, "GAATC": 1457990, "GAATG": 33587, "GAACA": 1378160, "GAACT": 825135, "GAACC": 618953, "GAACG": 165809, "GAAGA": 8859006, "GAAGT": 340717, "GAAGC": 261411, "GAAGG": 4272814, "GATAA": 2535796, "GATAT": 1669761, "GATAC": 1084148, "GATAG": 40843, "GATTA": 1775044, "GATTT": 1242595, "GATTC": 802228, "GATTG": 13537, "GATCA": 1766669, "GATCT": 1580114, "GATCC": 681061, "GATCG": 3374745, "GATGA": 34151, "GATGT": 1163036, "GATGC": 5473, "GATGG": 33597, "GACAA": 1312626, "GACAT": 771364, "GACAC": 476799, "GACAG": 15037, "GACTA": 824886, "GACTT": 500541, "GACTC": 337609, "GACTG": 4144, "GACCA": 663630, "GACCT": 423128, "GACCC": 312414, "GACCG": 96124, "GACGA": 159660, "GACGT": 144173, "GACGC": 59429, "GACGG": 9050, "GAGAA": 7321294, "GAGAT": 2676072, "GAGAC": 1108258, "GAGAG": 7846854, "GAGTA": 430687, "GAGTT": 306679, "GAGTC": 340801, "GAGTG": 1401898, "GAGCA": 317662, "GAGCT": 267703, "GAGCC": 232096, "GAGCG": 2470940, "GAGGA": 9459762, "GAGGT": 596652, "GAGGC": 460374, "GAGGG": 5924289, "GTAAA": 1091818, "GTAAT": 641292, "GTAAC": 385898, "GTAAG": 35239, "GTATA": 683101, "GTATT": 513445, "GTATC": 670932, "GTATG": 8555, "GTACA": 444411, "GTACT": 254994, "GTACC": 177473, "GTACG": 83515, "GTAGA": 610836, "GTAGT": 1034758, "GTAGC": 5243, "GTAGG": 1792203, "GTTAA": 599688, "GTTAT": 504893, "GTTAC": 264408, "GTTAG": 17240, "GTTTA": 444601, "GTTTT": 423193, "GTTTC": 254007, "GTTTG": 24138, "GTTCA": 292403, "GTTCT": 199201, "GTTCC": 133455, "GTTCG": 40222, "GTTGA": 8857, "GTTGT": 85663, "GTTGC": 109889, "GTTGG": 16168, "GTCAA": 624958, "GTCAT": 422736, "GTCAC": 273769, "GTCAG": 8757, "GTCTA": 297745, "GTCTT": 208758, "GTCTC": 243098, "GTCTG": 7236, "GTCCA": 217492, "GTCCT": 168672, "GTCCC": 131325, "GTCCG": 66020, "GTCGA": 412996, "GTCGT": 2304590, "GTCGC": 663959, "GTCGG": 41476, "GTGAA": 22958, "GTGAT": 1206634, "GTGAC": 9954, "GTGAG": 36060, "GTGTA": 2539080, "GTGTT": 43682, "GTGTC": 444199, "GTGTG": 2738111, "GTGCA": 6222, "GTGCT": 95956, "GTGCC": 5266, "GTGCG": 4038, "GTGGA": 59720, "GTGGT": 850353, "GTGGC": 8592, "GTGGG": 107246, "GCAAA": 595580, "GCAAT": 358299, "GCAAC": 209025, "GCAAG": 10083, "GCATA": 333598, "GCATT": 237625, "GCATC": 141196, "GCATG": 3403, "GCACA": 200376, "GCACT": 145829, "GCACC": 90083, "GCACG": 36659, "GCAGA": 21015, "GCAGT": 6135, "GCAGC": 4016, "GCAGG": 10162, "GCTAA": 391888, "GCTAT": 358775, "GCTAC": 171934, "GCTAG": 4263, "GCTTA": 232341, "GCTTT": 235444, "GCTTC": 129450, "GCTTG": 2862, "GCTCA": 187040, "GCTCT": 260961, "GCTCC": 87862, "GCTCG": 45210, "GCTGA": 3772, "GCTGT": 4870, "GCTGC": 2076, "GCTGG": 2667, "GCCAA": 309618, "GCCAT": 462604, "GCCAC": 133325, "GCCAG": 5437, "GCCTA": 164937, "GCCTT": 129877, "GCCTC": 82517, "GCCTG": 2313, "GCCCA": 148333, "GCCCT": 133878, "GCCCC": 115357, "GCCCG": 38726, "GCCGA": 62801, "GCCGT": 499175, "GCCGC": 24229, "GCCGG": 3124, "GCGAA": 117985, "GCGAT": 75281, "GCGAC": 45826, "GCGAG": 11007, "GCGTA": 69368, "GCGTT": 43914, "GCGTC": 2312155, "GCGTG": 13079, "GCGCA": 34804, "GCGCT": 25795, "GCGCC": 26807, "GCGCG": 23147, "GCGGA": 29148, "GCGGT": 3834, "GCGGC": 3650, "GCGGG": 15901, "GGAAA": 8512027, "GGAAT": 2549314, "GGAAC": 1211400, "GGAAG": 9329430, "GGATA": 2270402, "GGATT": 1637119, "GGATC": 1633361, "GGATG": 38366, "GGACA": 1116716, "GGACT": 692464, "GGACC": 606612, "GGACG": 69354, "GGAGA": 11141137, "GGAGT": 654276, "GGAGC": 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--trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs/fastp/Rio3.fastp.json --html output/13-wgbs/fastp/Rio3.fastp.html " }, "RP4 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 81176642, "total_bases": 12257672942, "q20_bases": 12113072958, "q30_bases": 11721362139, "q20_rate": 0.988203, "q30_rate": 0.956247, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.211639 }, "after_filtering": { "total_reads": 79945874, "total_bases": 11077433848, "q20_bases": 10982648499, "q30_bases": 10671919288, "q20_rate": 0.991443, "q30_rate": 0.963393, "read1_mean_length": 138, "read2_mean_length": 138, "gc_content": 0.188384 } }, "filtering_result": { "passed_filter_reads": 79945874, "low_quality_reads": 614450, "too_many_N_reads": 1462, "too_short_reads": 614856, "too_long_reads": 0 }, "duplication": { "rate": 0.043348 }, "insert_size": { 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35646011, "TACAT": 29192458, "TACAC": 15000888, "TACAG": 130634, "TACTA": 21200679, "TACTT": 16157825, "TACTC": 7439404, "TACTG": 60095, "TACCA": 12360839, "TACCT": 8337412, "TACCC": 5045030, "TACCG": 631316, "TACGA": 1385245, "TACGT": 1002089, "TACGC": 522709, "TACGG": 5495, "TAGAA": 135129, "TAGAT": 3025114, "TAGAC": 63053, "TAGAG": 64311, "TAGTA": 75534, "TAGTT": 71234, "TAGTC": 45974, "TAGTG": 19468, "TAGCA": 44062, "TAGCT": 34860, "TAGCC": 26190, "TAGCG": 5687, "TAGGA": 58205, "TAGGT": 21754, "TAGGC": 10126, "TAGGG": 1603189, "TTAAA": 64548777, "TTAAT": 40938042, "TTAAC": 21124583, "TTAAG": 245006, "TTATA": 46220196, "TTATT": 38175684, "TTATC": 17680094, "TTATG": 118547, "TTACA": 25812156, "TTACT": 15289771, "TTACC": 8714359, "TTACG": 1000427, "TTAGA": 519203, "TTAGT": 60669, "TTAGC": 31634, "TTAGG": 20167, "TTTAA": 52752641, "TTTAT": 41617658, "TTTAC": 20671166, "TTTAG": 259857, "TTTTA": 45246894, "TTTTT": 42546522, "TTTTC": 21624675, "TTTTG": 155427, "TTTCA": 25499406, "TTTCT": 18257087, "TTTCC": 9681628, "TTTCG": 874817, "TTTGA": 143551, "TTTGT": 132134, "TTTGC": 46905, "TTTGG": 35701, "TTCAA": 27828270, "TTCAT": 20630496, "TTCAC": 10304295, "TTCAG": 109763, "TTCTA": 18354692, "TTCTT": 15251030, "TTCTC": 8091168, "TTCTG": 66482, "TTCCA": 10284391, "TTCCT": 7716074, "TTCCC": 4434787, "TTCCG": 638595, "TTCGA": 1099789, "TTCGT": 725345, "TTCGC": 359655, "TTCGG": 9536, "TTGAA": 170177, "TTGAT": 116528, "TTGAC": 59541, "TTGAG": 22975, "TTGTA": 114467, "TTGTT": 120445, "TTGTC": 76651, "TTGTG": 906371, "TTGCA": 54345, "TTGCT": 42398, "TTGCC": 47416, "TTGCG": 6186, "TTGGA": 27177, "TTGGT": 23353, "TTGGC": 12898, "TTGGG": 32589, "TCAAA": 38135739, "TCAAT": 24186317, "TCAAC": 12907054, "TCAAG": 103788, "TCATA": 22468896, "TCATT": 20150791, "TCATC": 9253060, "TCATG": 65663, "TCACA": 13424507, "TCACT": 9916428, "TCACC": 5588071, "TCACG": 449909, "TCAGA": 234770, "TCAGT": 54747, "TCAGC": 26395, "TCAGG": 18600, "TCTAA": 21994309, "TCTAT": 19769111, "TCTAC": 9777135, "TCTAG": 145570, "TCTTA": 14484718, "TCTTT": 14952831, "TCTTC": 8177906, "TCTTG": 48191, "TCTCA": 8766745, "TCTCT": 12353448, "TCTCC": 4519484, "TCTCG": 1289055, "TCTGA": 91662, "TCTGT": 78099, "TCTGC": 32866, "TCTGG": 95861, "TCCAA": 12958900, "TCCAT": 9571737, "TCCAC": 5358108, "TCCAG": 73398, "TCCTA": 8172530, "TCCTT": 6568951, "TCCTC": 4269577, "TCCTG": 36285, "TCCCA": 5242109, "TCCCT": 3820537, "TCCCC": 3180399, "TCCCG": 289031, "TCCGA": 920478, "TCCGT": 578132, "TCCGC": 250885, "TCCGG": 5439, "TCGAA": 1817080, "TCGAT": 1291779, "TCGAC": 633010, "TCGAG": 11264, "TCGTA": 1057055, "TCGTT": 690987, "TCGTC": 461880, "TCGTG": 1937030, "TCGCA": 447240, "TCGCT": 417267, "TCGCC": 913000, "TCGCG": 140295, "TCGGA": 2857653, "TCGGT": 754356, "TCGGC": 8798, "TCGGG": 37493, "TGAAA": 294123, "TGAAT": 159432, "TGAAC": 85701, "TGAAG": 49424, "TGATA": 170188, "TGATT": 123965, "TGATC": 63210, "TGATG": 18313, "TGACA": 97272, "TGACT": 55755, "TGACC": 48835, "TGACG": 8629, "TGAGA": 61168, "TGAGT": 20451, "TGAGC": 14681, "TGAGG": 70329, "TGTAA": 158793, "TGTAT": 131215, "TGTAC": 58460, "TGTAG": 2430962, "TGTTA": 94246, "TGTTT": 147065, "TGTTC": 61079, "TGTTG": 42161, "TGTCA": 75715, "TGTCT": 56618, "TGTCC": 36736, "TGTCG": 157981, "TGTGA": 23204, "TGTGT": 913816, "TGTGC": 95904, "TGTGG": 1200766, "TGCAA": 74262, "TGCAT": 62964, "TGCAC": 37332, "TGCAG": 21542, "TGCTA": 46076, "TGCTT": 47514, "TGCTC": 142238, "TGCTG": 16813, "TGCCA": 36821, "TGCCT": 30166, "TGCCC": 25242, "TGCCG": 23691, "TGCGA": 6983, "TGCGT": 6946, "TGCGC": 5772, "TGCGG": 5331, "TGGAA": 83206, "TGGAT": 24113, "TGGAC": 12622, "TGGAG": 110283, "TGGTA": 1059023, "TGGTT": 49893, "TGGTC": 764669, "TGGTG": 35280, "TGGCA": 17840, "TGGCT": 16766, "TGGCC": 13534, "TGGCG": 7914, "TGGGA": 105047, "TGGGT": 23166, "TGGGC": 15924, "TGGGG": 170276, "CAAAA": 59377835, "CAAAT": 34902371, "CAAAC": 19616323, "CAAAG": 212495, "CAATA": 35654477, "CAATT": 22940016, "CAATC": 11978196, "CAATG": 99142, "CAACA": 19452256, "CAACT": 11328830, "CAACC": 7137610, "CAACG": 596397, "CAAGA": 251709, "CAAGT": 54633, "CAAGC": 32365, "CAAGG": 22771, "CATAA": 30202686, "CATAT": 27885486, "CATAC": 13786034, "CATAG": 184507, "CATTA": 23752686, "CATTT": 23300564, "CATTC": 8942317, "CATTG": 63063, "CATCA": 14007535, "CATCT": 8685580, "CATCC": 4900906, "CATCG": 500917, "CATGA": 96521, "CATGT": 77366, "CATGC": 38483, "CATGG": 16240, "CACAA": 19561366, "CACAT": 13455638, "CACAC": 10658475, "CACAG": 93014, "CACTA": 13391579, "CACTT": 8845720, "CACTC": 5757059, "CACTG": 48752, "CACCA": 8285548, "CACCT": 5547232, "CACCC": 3867054, "CACCG": 352166, "CACGA": 790173, "CACGT": 494178, "CACGC": 260412, "CACGG": 6488, "CAGAA": 95016, "CAGAT": 549939, "CAGAC": 58042, "CAGAG": 36927, "CAGTA": 49697, "CAGTT": 42998, "CAGTC": 29291, "CAGTG": 34976, "CAGCA": 38530, "CAGCT": 28895, "CAGCC": 29739, "CAGCG": 5741, "CAGGA": 30566, "CAGGT": 14859, "CAGGC": 16049, "CAGGG": 26282, "CTAAA": 30467827, "CTAAT": 19427050, "CTAAC": 11122713, "CTAAG": 158930, "CTATA": 25976143, "CTATT": 16847444, "CTATC": 9890883, "CTATG": 65699, "CTACA": 13374840, "CTACT": 8173662, "CTACC": 4926861, "CTACG": 478932, "CTAGA": 299826, "CTAGT": 32309, "CTAGC": 18448, "CTAGG": 23260, "CTTAA": 18785060, "CTTAT": 14291441, "CTTAC": 8057736, "CTTAG": 118422, "CTTTA": 18174765, "CTTTT": 15643839, "CTTTC": 8607401, "CTTTG": 59187, "CTTCA": 9761966, "CTTCT": 6861446, "CTTCC": 4469357, "CTTCG": 405871, "CTTGA": 60183, "CTTGT": 41679, "CTTGC": 22864, "CTTGG": 15596, "CTCAA": 11494595, "CTCAT": 7170227, "CTCAC": 4980636, "CTCAG": 92151, "CTCTA": 9997398, "CTCTT": 6494248, "CTCTC": 8983606, "CTCTG": 57278, "CTCCA": 5207791, "CTCCT": 3793423, "CTCCC": 2802428, "CTCCG": 294494, "CTCGA": 796585, "CTCGT": 422440, "CTCGC": 289845, "CTCGG": 766125, "CTGAA": 101224, "CTGAT": 66301, "CTGAC": 38544, "CTGAG": 37257, "CTGTA": 70043, "CTGTT": 58314, "CTGTC": 36153, "CTGTG": 36560, "CTGCA": 41440, "CTGCT": 31089, "CTGCC": 22966, "CTGCG": 5223, "CTGGA": 41698, "CTGGT": 11743, "CTGGC": 12408, "CTGGG": 78537, "CCAAA": 20613697, "CCAAT": 12062204, "CCAAC": 6992835, "CCAAG": 85142, "CCATA": 12586935, "CCATT": 9360420, "CCATC": 5101822, "CCATG": 49386, "CCACA": 7498320, "CCACT": 4709649, "CCACC": 4448367, "CCACG": 284183, "CCAGA": 175751, "CCAGT": 29707, "CCAGC": 28684, "CCAGG": 21210, "CCTAA": 11201899, "CCTAT": 9494143, "CCTAC": 5116260, "CCTAG": 83035, "CCTTA": 8069547, "CCTTT": 7780791, "CCTTC": 3840837, "CCTTG": 31522, "CCTCA": 4618824, "CCTCT": 4358301, "CCTCC": 2873148, "CCTCG": 298757, "CCTGA": 45773, "CCTGT": 36957, "CCTGC": 23074, "CCTGG": 18655, "CCCAA": 8178835, "CCCAT": 4875490, "CCCAC": 3864968, "CCCAG": 85486, "CCCTA": 5716954, "CCCTT": 3928042, "CCCTC": 2841476, "CCCTG": 30237, "CCCCA": 4538093, "CCCCT": 3394776, "CCCCC": 7364449, "CCCCG": 280109, "CCCGA": 553902, "CCCGT": 266214, "CCCGC": 202687, "CCCGG": 19685, "CCGAA": 1233906, "CCGAT": 862075, "CCGAC": 486849, "CCGAG": 15452, "CCGTA": 1252874, "CCGTT": 428272, "CCGTC": 366233, "CCGTG": 13519, "CCGCA": 248861, "CCGCT": 235174, "CCGCC": 227005, "CCGCG": 101731, "CCGGA": 11080, "CCGGT": 11388, "CCGGC": 4934, "CCGGG": 13758, "CGAAA": 2840424, "CGAAT": 1577750, "CGAAC": 1009772, "CGAAG": 34664, "CGATA": 1662637, "CGATT": 1104923, "CGATC": 866768, "CGATG": 9153, "CGACA": 765250, "CGACT": 538207, "CGACC": 443958, "CGACG": 257423, "CGAGA": 25243, "CGAGT": 13493, "CGAGC": 7747, "CGAGG": 15225, "CGTAA": 1448205, "CGTAT": 1705536, "CGTAC": 639808, "CGTAG": 19002, "CGTTA": 888096, "CGTTT": 781571, "CGTTC": 357240, "CGTTG": 14297, "CGTCA": 582920, "CGTCT": 467808, "CGTCC": 290195, "CGTCG": 2227436, "CGTGA": 11160, "CGTGT": 1885613, "CGTGC": 7570, "CGTGG": 56036, "CGCAA": 621894, "CGCAT": 415358, "CGCAC": 261532, "CGCAG": 12121, "CGCTA": 527834, "CGCTT": 370942, "CGCTC": 251260, "CGCTG": 4695, "CGCCA": 357450, "CGCCT": 205472, "CGCCC": 204567, "CGCCG": 709243, "CGCGA": 226639, "CGCGT": 129379, "CGCGC": 101117, "CGCGG": 5038, "CGGAA": 2847076, "CGGAT": 8940, "CGGAC": 7608, "CGGAG": 23055, "CGGTA": 7678, "CGGTT": 14637, "CGGTC": 5021, "CGGTG": 738406, "CGGCA": 7092, "CGGCT": 6522, "CGGCC": 5770, "CGGCG": 5063, "CGGGA": 37962, "CGGGT": 10583, "CGGGC": 5659, "CGGGG": 28810, "GAAAA": 8407219, "GAAAT": 3620633, "GAAAC": 1956304, "GAAAG": 5338049, "GAATA": 3286390, "GAATT": 2269697, "GAATC": 1592854, "GAATG": 67904, "GAACA": 1798254, "GAACT": 1079008, "GAACC": 797837, "GAACG": 217277, "GAAGA": 10235676, "GAAGT": 390510, "GAAGC": 337099, "GAAGG": 5356605, "GATAA": 3193219, "GATAT": 2128248, "GATAC": 1355939, "GATAG": 65467, "GATTA": 2112766, "GATTT": 1582135, "GATTC": 937771, "GATTG": 938853, "GATCA": 1718296, "GATCT": 1763515, "GATCC": 655133, "GATCG": 3203629, "GATGA": 64414, "GATGT": 25621, "GATGC": 13596, "GATGG": 62041, "GACAA": 1677693, "GACAT": 992694, "GACAC": 601193, "GACAG": 32039, "GACTA": 1051656, "GACTT": 650389, "GACTC": 422947, "GACTG": 23600, "GACCA": 825359, "GACCT": 467615, "GACCC": 371324, "GACCG": 121734, "GACGA": 203890, "GACGT": 168797, "GACGC": 77070, "GACGG": 13295, "GAGAA": 9353218, "GAGAT": 3100123, "GAGAC": 1430852, "GAGAG": 9846867, "GAGTA": 537175, "GAGTT": 366200, "GAGTC": 319360, "GAGTG": 1255843, "GAGCA": 423830, "GAGCT": 339605, "GAGCC": 284357, "GAGCG": 2309671, "GAGGA": 11792649, "GAGGT": 664657, "GAGGC": 587709, "GAGGG": 7271759, "GTAAA": 1314617, "GTAAT": 781222, "GTAAC": 482514, "GTAAG": 41386, "GTATA": 837261, "GTATT": 628221, "GTATC": 925804, "GTATG": 16174, "GTACA": 543867, "GTACT": 316532, "GTACC": 207505, "GTACG": 102272, "GTAGA": 1771700, "GTAGT": 20598, "GTAGC": 11201, "GTAGG": 1645520, "GTTAA": 711268, "GTTAT": 591755, "GTTAC": 315350, "GTTAG": 16464, "GTTTA": 547037, "GTTTT": 520611, "GTTTC": 293962, "GTTTG": 27322, "GTTCA": 338525, "GTTCT": 233892, "GTTCC": 152273, "GTTCG": 47932, "GTTGA": 17464, "GTTGT": 59888, "GTTGC": 33712, "GTTGG": 23701, "GTCAA": 566260, "GTCAT": 447251, "GTCAC": 282977, "GTCAG": 17248, "GTCTA": 356519, "GTCTT": 248606, "GTCTC": 195229, "GTCTG": 14596, "GTCCA": 225546, "GTCCT": 169123, "GTCCC": 127303, "GTCCG": 79949, "GTCGA": 247274, "GTCGT": 2090646, "GTCGC": 704572, "GTCGG": 43149, "GTGAA": 42214, "GTGAT": 19671, "GTGAC": 12136, "GTGAG": 49029, "GTGTA": 2497622, "GTGTT": 41378, "GTGTC": 155981, "GTGTG": 1301910, "GTGCA": 16647, "GTGCT": 123085, "GTGCC": 11348, "GTGCG": 5920, "GTGGA": 81497, "GTGGT": 1892417, "GTGGC": 17703, "GTGGG": 131247, "GCAAA": 777287, "GCAAT": 459187, "GCAAC": 272253, "GCAAG": 21489, "GCATA": 426415, "GCATT": 305554, "GCATC": 179251, "GCATG": 13707, "GCACA": 258149, "GCACT": 184992, "GCACC": 118121, "GCACG": 47561, "GCAGA": 37737, "GCAGT": 19497, "GCAGC": 13029, "GCAGG": 24133, "GCTAA": 500912, "GCTAT": 443964, "GCTAC": 220017, "GCTAG": 9880, "GCTTA": 295042, "GCTTT": 299654, "GCTTC": 168469, "GCTTG": 11629, "GCTCA": 236256, "GCTCT": 315166, "GCTCC": 111241, "GCTCG": 57576, "GCTGA": 16723, "GCTGT": 13258, "GCTGC": 10739, "GCTGG": 17173, "GCCAA": 375065, "GCCAT": 331627, "GCCAC": 168033, "GCCAG": 17109, "GCCTA": 214044, "GCCTT": 169008, "GCCTC": 113331, "GCCTG": 16128, "GCCCA": 181178, "GCCCT": 143449, "GCCCC": 136359, "GCCCG": 52895, "GCCGA": 80494, "GCCGT": 657739, "GCCGC": 30371, "GCCGG": 5778, "GCGAA": 150213, "GCGAT": 94117, "GCGAC": 57130, "GCGAG": 15709, "GCGTA": 83951, "GCGTT": 52744, "GCGTC": 2152004, "GCGTG": 12605, "GCGCA": 43978, "GCGCT": 33544, "GCGCC": 34328, "GCGCG": 27904, "GCGGA": 37133, "GCGGT": 5222, "GCGGC": 5617, "GCGGG": 20784, "GGAAA": 10246744, "GGAAT": 3091724, "GGAAC": 1586015, "GGAAG": 10716687, "GGATA": 2877147, "GGATT": 1960200, "GGATC": 1584688, "GGATG": 70494, "GGACA": 1432568, "GGACT": 892278, "GGACC": 751933, "GGACG": 89304, "GGAGA": 13830221, "GGAGT": 735578, "GGAGC": 637982, "GGAGG": 10442526, "GGTAA": 556998, "GGTAT": 281895, "GGTAC": 250083, "GGTAG": 1051936, "GGTTA": 350638, "GGTTT": 221723, "GGTTC": 167428, "GGTTG": 61642, "GGTCA": 353424, "GGTCT": 149671, "GGTCC": 136961, "GGTCG": 756771, "GGTGA": 44512, "GGTGT": 59144, "GGTGC": 29497, "GGTGG": 838827, "GGCAA": 462498, "GGCAT": 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666006, "GACAT": 424454, "GACAC": 253832, "GACAG": 15562, "GACTA": 438233, "GACTT": 300711, "GACTC": 166588, "GACTG": 12353, "GACCA": 315592, "GACCT": 195853, "GACCC": 167241, "GACCG": 77701, "GACGA": 134888, "GACGT": 91716, "GACGC": 54468, "GACGG": 3992, "GAGAA": 748081, "GAGAT": 397169, "GAGAC": 176276, "GAGAG": 471945, "GAGTA": 83215, "GAGTT": 65191, "GAGTC": 45945, "GAGTG": 274861, "GAGCA": 64535, "GAGCT": 51932, "GAGCC": 44203, "GAGCG": 120924, "GAGGA": 548132, "GAGGT": 59403, "GAGGC": 52190, "GAGGG": 286135, "GTAAA": 922541, "GTAAT": 556586, "GTAAC": 346279, "GTAAG": 14432, "GTATA": 643600, "GTATT": 499087, "GTATC": 501378, "GTATG": 10619, "GTACA": 360323, "GTACT": 213465, "GTACC": 145512, "GTACG": 91285, "GTAGA": 480013, "GTAGT": 10601, "GTAGC": 6880, "GTAGG": 269327, "GTTAA": 500782, "GTTAT": 429737, "GTTAC": 222499, "GTTAG": 8249, "GTTTA": 419442, "GTTTT": 409576, "GTTTC": 222297, "GTTTG": 16060, "GTTCA": 223038, "GTTCT": 159579, "GTTCC": 107603, "GTTCG": 40403, "GTTGA": 12327, "GTTGT": 16857, "GTTGC": 13382, "GTTGG": 10913, "GTCAA": 329169, "GTCAT": 292660, "GTCAC": 185511, "GTCAG": 10603, "GTCTA": 268532, "GTCTT": 179619, "GTCTC": 130854, "GTCTG": 10692, "GTCCA": 135328, "GTCCT": 103844, "GTCCC": 85265, "GTCCG": 71819, "GTCGA": 132427, "GTCGT": 342218, "GTCGC": 284856, "GTCGG": 8587, "GTGAA": 20648, "GTGAT": 14330, "GTGAC": 9198, "GTGAG": 18303, "GTGTA": 489995, "GTGTT": 18078, "GTGTC": 50097, "GTGTG": 283472, "GTGCA": 11265, "GTGCT": 19442, "GTGCC": 8023, "GTGCG": 2602, "GTGGA": 18763, "GTGGT": 540348, "GTGGC": 10755, "GTGGG": 24075, "GCAAA": 439875, "GCAAT": 272843, "GCAAC": 162114, "GCAAG": 11874, "GCATA": 272861, "GCATT": 202184, "GCATC": 113527, "GCATG": 11444, "GCACA": 156230, "GCACT": 112695, "GCACC": 67777, "GCACG": 40060, "GCAGA": 16330, "GCAGT": 13118, "GCAGC": 9794, "GCAGG": 13711, "GCTAA": 309358, "GCTAT": 270072, "GCTAC": 134516, "GCTAG": 6181, "GCTTA": 190477, "GCTTT": 199298, "GCTTC": 110825, "GCTTG": 9421, "GCTCA": 139305, "GCTCT": 125287, "GCTCC": 63551, "GCTCG": 42258, "GCTGA": 13828, "GCTGT": 10950, "GCTGC": 9416, "GCTGG": 15043, "GCCAA": 192193, "GCCAT": 184964, "GCCAC": 98013, "GCCAG": 12209, "GCCTA": 127680, "GCCTT": 95000, "GCCTC": 67235, "GCCTG": 14671, "GCCCA": 100000, "GCCCT": 76113, "GCCCC": 76868, "GCCCG": 36643, "GCCGA": 60988, "GCCGT": 278137, "GCCGC": 22404, "GCCGG": 3073, "GCGAA": 112503, "GCGAT": 79112, "GCGAC": 45135, "GCGAG": 3724, "GCGTA": 65347, "GCGTT": 42314, "GCGTC": 290849, "GCGTG": 4237, "GCGCA": 34602, "GCGCT": 27886, "GCGCC": 27406, "GCGCG": 19790, "GCGGA": 4362, "GCGGT": 2391, "GCGGC": 2552, "GCGGG": 4871, "GGAAA": 873384, "GGAAT": 350792, "GGAAC": 160101, "GGAAG": 379953, "GGATA": 350752, "GGATT": 264195, "GGATC": 175803, "GGATG": 22368, "GGACA": 153675, "GGACT": 101356, "GGACC": 84511, "GGACG": 9709, "GGAGA": 522957, "GGAGT": 59179, "GGAGC": 44920, "GGAGG": 314000, "GGTAA": 74428, "GGTAT": 44501, "GGTAC": 34124, "GGTAG": 255042, "GGTTA": 53769, "GGTTT": 44987, "GGTTC": 28252, "GGTTG": 17125, "GGTCA": 49312, "GGTCT": 25160, "GGTCC": 20496, "GGTCG": 278815, "GGTGA": 16713, "GGTGT": 11994, "GGTGC": 7607, "GGTGG": 282672, "GGCAA": 57636, "GGCAT": 36473, "GGCAC": 25973, "GGCAG": 15504, "GGCTA": 46947, "GGCTT": 30827, "GGCTC": 25469, "GGCTG": 15401, "GGCCA": 41643, "GGCCT": 24744, "GGCCC": 23134, "GGCCG": 5560, "GGCGA": 6008, "GGCGT": 4407, "GGCGC": 3869, "GGCGG": 5754, "GGGAA": 638632, "GGGAT": 199755, "GGGAC": 82864, "GGGAG": 297509, "GGGTA": 42220, "GGGTT": 36107, "GGGTC": 24211, "GGGTG": 22589, "GGGCA": 33259, "GGGCT": 28139, "GGGCC": 22356, "GGGCG": 6243, "GGGGA": 308863, "GGGGT": 38228, "GGGGC": 22542, "GGGGG": 1189550 }, "overrepresented_sequences": {} }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/RP4 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/RP4 (paired)_R2.fastq.gz -o output/13-wgbs/trimmed/Rio4_R1.fq.gz -O output/13-wgbs/trimmed/Rio4_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs/fastp/Rio4.fastp.json --html output/13-wgbs/fastp/Rio4.fastp.html " }, "RP5 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 66760720, "total_bases": 10080868720, "q20_bases": 9947606339, "q30_bases": 9610873513, "q20_rate": 0.986781, "q30_rate": 0.953378, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.216155 }, "after_filtering": { "total_reads": 65546394, "total_bases": 9034155486, "q20_bases": 8952579332, "q30_bases": 8697272677, "q20_rate": 0.99097, "q30_rate": 0.96271, "read1_mean_length": 137, "read2_mean_length": 137, "gc_content": 0.189349 } }, "filtering_result": { "passed_filter_reads": 65546394, "low_quality_reads": 506754, "too_many_N_reads": 1136, "too_short_reads": 706436, "too_long_reads": 0 }, "duplication": { "rate": 0.0391718 }, "insert_size": { "peak": 176, "unknown": 664530, "histogram": [ 0, 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1743451, "TTAAA": 51994145, "TTAAT": 33532294, "TTAAC": 17207835, "TTAAG": 232783, "TTATA": 38245070, "TTATT": 32080430, "TTATC": 14701363, "TTATG": 91693, "TTACA": 21207434, "TTACT": 12863248, "TTACC": 7115281, "TTACG": 825133, "TTAGA": 514895, "TTAGT": 52464, "TTAGC": 19817, "TTAGG": 14927, "TTTAA": 43093432, "TTTAT": 34925786, "TTTAC": 17101695, "TTTAG": 243048, "TTTTA": 37653017, "TTTTT": 36096400, "TTTTC": 18182149, "TTTTG": 93279, "TTTCA": 21158532, "TTTCT": 15422399, "TTTCC": 8087080, "TTTCG": 735535, "TTTGA": 105143, "TTTGT": 77536, "TTTGC": 30468, "TTTGG": 17844, "TTCAA": 22946217, "TTCAT": 17172159, "TTCAC": 8626396, "TTCAG": 92273, "TTCTA": 15248789, "TTCTT": 12809542, "TTCTC": 6875347, "TTCTG": 41129, "TTCCA": 8427317, "TTCCT": 6421509, "TTCCC": 3662092, "TTCCG": 535952, "TTCGA": 896293, "TTCGT": 619333, "TTCGC": 300315, "TTCGG": 11165, "TTGAA": 119447, "TTGAT": 88415, "TTGAC": 42399, "TTGAG": 8628, "TTGTA": 66702, "TTGTT": 90919, "TTGTC": 81499, "TTGTG": 19327, "TTGCA": 32618, "TTGCT": 25593, "TTGCC": 118435, "TTGCG": 4347, "TTGGA": 14065, "TTGGT": 14511, "TTGGC": 4476, "TTGGG": 19329, "TCAAA": 31187061, "TCAAT": 20017828, "TCAAC": 10520849, "TCAAG": 84756, "TCATA": 18395677, "TCATT": 16810280, "TCATC": 7709055, "TCATG": 48153, "TCACA": 11114860, "TCACT": 8686092, "TCACC": 4585049, "TCACG": 371382, "TCAGA": 219561, "TCAGT": 84998, "TCAGC": 13597, "TCAGG": 6706, "TCTAA": 17902317, "TCTAT": 16920494, "TCTAC": 8113605, "TCTAG": 135746, "TCTTA": 12036628, "TCTTT": 12685529, "TCTTC": 6815509, "TCTTG": 31108, "TCTCA": 7388540, "TCTCT": 11598561, "TCTCC": 3941350, "TCTCG": 1065720, "TCTGA": 61556, "TCTGT": 129535, "TCTGC": 17910, "TCTGG": 67908, "TCCAA": 10478458, "TCCAT": 7904645, "TCCAC": 4381055, "TCCAG": 56142, "TCCTA": 6743636, "TCCTT": 5586820, "TCCTC": 3552787, "TCCTG": 18873, "TCCCA": 4258799, "TCCCT": 3308111, "TCCCC": 2598761, "TCCCG": 239633, "TCCGA": 755026, "TCCGT": 567424, "TCCGC": 207049, "TCCGG": 4456, "TCGAA": 1476602, "TCGAT": 1235888, 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4545832, "CACCC": 3101962, "CACCG": 295758, "CACGA": 648785, "CACGT": 409896, "CACGC": 214324, "CACGG": 3552, "CAGAA": 58974, "CAGAT": 559209, "CAGAC": 39542, "CAGAG": 15346, "CAGTA": 30282, "CAGTT": 32594, "CAGTC": 18458, "CAGTG": 64402, "CAGCA": 19108, "CAGCT": 14101, "CAGCC": 14288, "CAGCG": 3719, "CAGGA": 12009, "CAGGT": 3862, "CAGGC": 3219, "CAGGG": 12373, "CTAAA": 24515507, "CTAAT": 15914456, "CTAAC": 9015773, "CTAAG": 154597, "CTATA": 21943241, "CTATT": 14149208, "CTATC": 8329739, "CTATG": 46173, "CTACA": 10961976, "CTACT": 6792858, "CTACC": 4002643, "CTACG": 389556, "CTAGA": 301658, "CTAGT": 22525, "CTAGC": 10892, "CTAGG": 13237, "CTTAA": 15272974, "CTTAT": 11864012, "CTTAC": 6680515, "CTTAG": 114892, "CTTTA": 15087096, "CTTTT": 13207961, "CTTTC": 7242484, "CTTTG": 35834, "CTTCA": 8169169, "CTTCT": 5692922, "CTTCC": 3672338, "CTTCG": 335333, "CTTGA": 38251, "CTTGT": 26287, "CTTGC": 12102, "CTTGG": 6649, "CTCAA": 9421585, "CTCAT": 5936359, "CTCAC": 4256811, "CTCAG": 122574, 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"CCTGG": 3606, "CCCAA": 6468222, "CCCAT": 3940273, "CCCAC": 3118968, "CCCAG": 69480, "CCCTA": 4724247, "CCCTT": 3198988, "CCCTC": 2422278, "CCCTG": 20598, "CCCCA": 3597477, "CCCCT": 2744072, "CCCCC": 5928255, "CCCCG": 235815, "CCCGA": 442565, "CCCGT": 224626, "CCCGC": 164809, "CCCGG": 20908, "CCGAA": 994488, "CCGAT": 707942, "CCGAC": 397779, "CCGAG": 8580, "CCGTA": 952858, "CCGTT": 373207, "CCGTC": 357415, "CCGTG": 71114, "CCGCA": 204826, "CCGCT": 194339, "CCGCC": 181617, "CCGCG": 80948, "CCGGA": 8985, "CCGGT": 15811, "CCGGC": 2472, "CCGGG": 8830, "CGAAA": 2278237, "CGAAT": 1280440, "CGAAC": 807306, "CGAAG": 29953, "CGATA": 1358969, "CGATT": 884451, "CGATC": 905123, "CGATG": 6500, "CGACA": 622866, "CGACT": 442646, "CGACC": 395901, "CGACG": 205584, "CGAGA": 18370, "CGAGT": 7271, "CGAGC": 5990, "CGAGG": 9778, "CGTAA": 1180785, "CGTAT": 1331785, "CGTAC": 540043, "CGTAG": 21695, "CGTTA": 741356, "CGTTT": 663712, "CGTTC": 296068, "CGTTG": 29034, "CGTCA": 482079, "CGTCT": 441417, "CGTCC": 242169, "CGTCG": 2360533, "CGTGA": 9897, "CGTGT": 2097116, "CGTGC": 5534, "CGTGG": 107049, "CGCAA": 509683, "CGCAT": 344804, "CGCAC": 216925, "CGCAG": 7382, "CGCTA": 430049, "CGCTT": 309419, "CGCTC": 203076, "CGCTG": 2383, "CGCCA": 460929, "CGCCT": 168017, "CGCCC": 173377, "CGCCG": 485929, "CGCGA": 182326, "CGCGT": 107655, "CGCGC": 80824, "CGCGG": 3412, "CGGAA": 3007382, "CGGAT": 6077, "CGGAC": 5408, "CGGAG": 19875, "CGGTA": 6870, "CGGTT": 29871, "CGGTC": 4557, "CGGTG": 650635, "CGGCA": 4794, "CGGCT": 2563, "CGGCC": 3280, "CGGCG": 3811, "CGGGA": 36678, "CGGGT": 5518, "CGGGC": 2757, "CGGGG": 24030, "GAAAA": 6514635, "GAAAT": 2858598, "GAAAC": 1477337, "GAAAG": 4675486, "GAATA": 2553823, "GAATT": 1831151, "GAATC": 1457990, "GAATG": 33587, "GAACA": 1378160, "GAACT": 825135, "GAACC": 618953, "GAACG": 165809, "GAAGA": 8859006, "GAAGT": 340717, "GAAGC": 261411, "GAAGG": 4272814, "GATAA": 2535796, "GATAT": 1669761, "GATAC": 1084148, "GATAG": 40843, "GATTA": 1775044, "GATTT": 1242595, "GATTC": 802228, "GATTG": 13537, "GATCA": 1766669, "GATCT": 1580114, "GATCC": 681061, "GATCG": 3374745, "GATGA": 34151, "GATGT": 1163036, "GATGC": 5473, "GATGG": 33597, "GACAA": 1312626, "GACAT": 771364, "GACAC": 476799, "GACAG": 15037, "GACTA": 824886, "GACTT": 500541, "GACTC": 337609, "GACTG": 4144, "GACCA": 663630, "GACCT": 423128, "GACCC": 312414, "GACCG": 96124, "GACGA": 159660, "GACGT": 144173, "GACGC": 59429, "GACGG": 9050, "GAGAA": 7321294, "GAGAT": 2676072, "GAGAC": 1108258, "GAGAG": 7846854, "GAGTA": 430687, "GAGTT": 306679, "GAGTC": 340801, "GAGTG": 1401898, "GAGCA": 317662, "GAGCT": 267703, "GAGCC": 232096, "GAGCG": 2470940, "GAGGA": 9459762, "GAGGT": 596652, "GAGGC": 460374, "GAGGG": 5924289, "GTAAA": 1091818, "GTAAT": 641292, "GTAAC": 385898, "GTAAG": 35239, "GTATA": 683101, "GTATT": 513445, "GTATC": 670932, "GTATG": 8555, "GTACA": 444411, "GTACT": 254994, "GTACC": 177473, "GTACG": 83515, "GTAGA": 610836, "GTAGT": 1034758, "GTAGC": 5243, "GTAGG": 1792203, "GTTAA": 599688, "GTTAT": 504893, "GTTAC": 264408, "GTTAG": 17240, "GTTTA": 444601, "GTTTT": 423193, "GTTTC": 254007, "GTTTG": 24138, "GTTCA": 292403, "GTTCT": 199201, "GTTCC": 133455, "GTTCG": 40222, "GTTGA": 8857, "GTTGT": 85663, "GTTGC": 109889, "GTTGG": 16168, "GTCAA": 624958, "GTCAT": 422736, "GTCAC": 273769, "GTCAG": 8757, "GTCTA": 297745, "GTCTT": 208758, "GTCTC": 243098, "GTCTG": 7236, "GTCCA": 217492, "GTCCT": 168672, "GTCCC": 131325, "GTCCG": 66020, "GTCGA": 412996, "GTCGT": 2304590, "GTCGC": 663959, "GTCGG": 41476, "GTGAA": 22958, "GTGAT": 1206634, "GTGAC": 9954, "GTGAG": 36060, "GTGTA": 2539080, "GTGTT": 43682, "GTGTC": 444199, "GTGTG": 2738111, "GTGCA": 6222, "GTGCT": 95956, "GTGCC": 5266, "GTGCG": 4038, "GTGGA": 59720, "GTGGT": 850353, "GTGGC": 8592, "GTGGG": 107246, "GCAAA": 595580, "GCAAT": 358299, "GCAAC": 209025, "GCAAG": 10083, "GCATA": 333598, "GCATT": 237625, "GCATC": 141196, "GCATG": 3403, "GCACA": 200376, "GCACT": 145829, "GCACC": 90083, "GCACG": 36659, "GCAGA": 21015, "GCAGT": 6135, "GCAGC": 4016, "GCAGG": 10162, "GCTAA": 391888, "GCTAT": 358775, "GCTAC": 171934, "GCTAG": 4263, "GCTTA": 232341, "GCTTT": 235444, "GCTTC": 129450, "GCTTG": 2862, "GCTCA": 187040, "GCTCT": 260961, "GCTCC": 87862, "GCTCG": 45210, "GCTGA": 3772, "GCTGT": 4870, "GCTGC": 2076, "GCTGG": 2667, "GCCAA": 309618, "GCCAT": 462604, "GCCAC": 133325, "GCCAG": 5437, "GCCTA": 164937, "GCCTT": 129877, "GCCTC": 82517, "GCCTG": 2313, "GCCCA": 148333, "GCCCT": 133878, "GCCCC": 115357, "GCCCG": 38726, "GCCGA": 62801, "GCCGT": 499175, "GCCGC": 24229, "GCCGG": 3124, "GCGAA": 117985, "GCGAT": 75281, "GCGAC": 45826, "GCGAG": 11007, "GCGTA": 69368, "GCGTT": 43914, "GCGTC": 2312155, "GCGTG": 13079, "GCGCA": 34804, "GCGCT": 25795, "GCGCC": 26807, "GCGCG": 23147, "GCGGA": 29148, "GCGGT": 3834, "GCGGC": 3650, "GCGGG": 15901, "GGAAA": 8512027, "GGAAT": 2549314, "GGAAC": 1211400, "GGAAG": 9329430, "GGATA": 2270402, "GGATT": 1637119, "GGATC": 1633361, "GGATG": 38366, "GGACA": 1116716, "GGACT": 692464, "GGACC": 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495000, "TCCGC": 195431, "TCCGG": 1613, "TCGAA": 1412126, "TCGAT": 1027950, "TCGAC": 506213, "TCGAG": 2725, "TCGTA": 844351, "TCGTT": 566289, "TCGTC": 363973, "TCGTG": 331035, "TCGCA": 354655, "TCGCT": 329945, "TCGCC": 445266, "TCGCG": 109460, "TCGGA": 23980, "TCGGT": 227359, "TCGGC": 1439, "TCGGG": 6597, "TGAAA": 182244, "TGAAT": 102536, "TGAAC": 52599, "TGAAG": 8271, "TGATA": 115448, "TGATT": 80322, "TGATC": 39215, "TGATG": 307939, "TGACA": 61686, "TGACT": 33202, "TGACC": 28776, "TGACG": 5059, "TGAGA": 10820, "TGAGT": 2749, "TGAGC": 1404, "TGAGG": 14816, "TGTAA": 106338, "TGTAT": 87552, "TGTAC": 36180, "TGTAG": 728916, "TGTTA": 62817, "TGTTT": 80571, "TGTTC": 39097, "TGTTG": 10839, "TGTCA": 62932, "TGTCT": 34386, "TGTCC": 21825, "TGTCG": 166894, "TGTGA": 309645, "TGTGT": 314988, "TGTGC": 5378, "TGTGG": 28975, "TGCAA": 41717, "TGCAT": 36457, "TGCAC": 18805, "TGCAG": 1836, "TGCTA": 26603, "TGCTT": 23812, "TGCTC": 24921, "TGCTG": 1555, "TGCCA": 26587, "TGCCT": 11569, "TGCCC": 10165, "TGCCG": 23858, "TGCGA": 2833, "TGCGT": 2576, "TGCGC": 2061, "TGCGG": 844, "TGGAA": 12994, "TGGAT": 3290, "TGGAC": 1611, "TGGAG": 21607, "TGGTA": 2071, "TGGTT": 40752, "TGGTC": 252078, "TGGTG": 3965, "TGGCA": 1626, "TGGCT": 1561, "TGGCC": 1256, "TGGCG": 984, "TGGGA": 26514, "TGGGT": 3085, "TGGGC": 1690, "TGGGG": 38322, "CAAAA": 46058838, "CAAAT": 27343350, "CAAAC": 15193400, "CAAAG": 56703, "CAATA": 28228845, "CAATT": 18323597, "CAATC": 9471274, "CAATG": 64351, "CAACA": 15146574, "CAACT": 8862799, "CAACC": 5428233, "CAACG": 465405, "CAAGA": 43679, "CAAGT": 31259, "CAAGC": 14262, "CAAGG": 3048, "CATAA": 23763622, "CATAT": 22374265, "CATAC": 10979641, "CATAG": 31276, "CATTA": 18764478, "CATTT": 18891315, "CATTC": 7262192, "CATTG": 37211, "CATCA": 11223741, "CATCT": 6985671, "CATCC": 3823891, "CATCG": 398905, "CATGA": 61812, "CATGT": 47535, "CATGC": 20311, "CATGG": 1880, "CACAA": 15303652, "CACAT": 10797312, "CACAC": 8936121, "CACAG": 20138, "CACTA": 10942888, "CACTT": 7133886, "CACTC": 5026892, "CACTG": 25855, "CACCA": 6376613, "CACCT": 4367972, "CACCC": 2794738, "CACCG": 279554, "CACGA": 627254, "CACGT": 396042, "CACGC": 204231, "CACGG": 1727, "CAGAA": 41429, "CAGAT": 40152, "CAGAC": 16009, "CAGAG": 3344, "CAGTA": 25002, "CAGTT": 24267, "CAGTC": 15006, "CAGTG": 22590, "CAGCA": 13921, "CAGCT": 10664, "CAGCC": 8191, "CAGCG": 1272, "CAGGA": 3169, "CAGGT": 1659, "CAGGC": 1360, "CAGGG": 2463, "CTAAA": 23872178, "CTAAT": 15557827, "CTAAC": 8782780, "CTAAG": 24488, "CTATA": 21300792, "CTATT": 13814028, "CTATC": 8085939, "CTATG": 42167, "CTACA": 10638960, "CTACT": 6601217, "CTACC": 3862078, "CTACG": 379266, "CTAGA": 28972, "CTAGT": 19831, "CTAGC": 8414, "CTAGG": 10620, "CTTAA": 14888693, "CTTAT": 11602683, "CTTAC": 6508260, "CTTAG": 16103, "CTTTA": 14734749, "CTTTT": 12876118, "CTTTC": 6997467, "CTTTG": 29410, "CTTCA": 7860796, "CTTCT": 5512063, "CTTCC": 3429245, "CTTCG": 322832, "CTTGA": 34147, "CTTGT": 20826, "CTTGC": 9844, "CTTGG": 1867, "CTCAA": 9038418, "CTCAT": 5738870, "CTCAC": 4054911, "CTCAG": 33460, "CTCTA": 8253293, "CTCTT": 5232637, "CTCTC": 8294097, "CTCTG": 48476, "CTCCA": 4051996, "CTCCT": 2981899, "CTCCC": 2060999, "CTCCG": 261146, "CTCGA": 624917, "CTCGT": 333680, "CTCGC": 227413, "CTCGG": 226691, "CTGAA": 60984, "CTGAT": 41355, "CTGAC": 21622, "CTGAG": 12874, "CTGTA": 42275, "CTGTT": 34994, "CTGTC": 19034, "CTGTG": 24706, "CTGCA": 17977, "CTGCT": 13024, "CTGCC": 7517, "CTGCG": 1696, "CTGGA": 19751, "CTGGT": 1280, "CTGGC": 1045, "CTGGG": 44984, "CCAAA": 15742619, "CCAAT": 9407064, "CCAAC": 5281259, "CCAAG": 18942, "CCATA": 9909228, "CCATT": 7436587, "CCATC": 4000790, "CCATG": 27640, "CCACA": 5774782, "CCACT": 3650158, "CCACC": 3225320, "CCACG": 218867, "CCAGA": 27105, "CCAGT": 14595, "CCAGC": 7409, "CCAGG": 1787, "CCTAA": 8754422, "CCTAT": 7592498, "CCTAC": 4018181, "CCTAG": 12782, "CCTTA": 6397034, "CCTTT": 6223620, "CCTTC": 2978506, "CCTTG": 14180, "CCTCA": 3563631, "CCTCT": 3428519, "CCTCC": 2059069, "CCTCG": 239387, "CCTGA": 23687, "CCTGT": 17094, "CCTGC": 7817, "CCTGG": 1712, "CCCAA": 6104614, "CCCAT": 3779248, "CCCAC": 2794180, "CCCAG": 18131, "CCCTA": 4506877, "CCCTT": 2976579, "CCCTC": 2081158, "CCCTG": 13214, "CCCCA": 3205468, "CCCCT": 2433742, "CCCCC": 4304637, "CCCCG": 207895, "CCCGA": 420054, "CCCGT": 206396, "CCCGC": 150787, "CCCGG": 4884, "CCGAA": 950443, "CCGAT": 662857, "CCGAC": 379600, "CCGAG": 2118, "CCGTA": 671342, "CCGTT": 346464, "CCGTC": 303501, "CCGTG": 28098, "CCGCA": 191730, "CCGCT": 183315, "CCGCC": 165601, "CCGCG": 76342, "CCGGA": 3013, "CCGGT": 4724, "CCGGC": 1018, "CCGGG": 945, "CGAAA": 2183355, "CGAAT": 1239555, "CGAAC": 777057, "CGAAG": 5748, "CGATA": 1315784, "CGATT": 854967, "CGATC": 697316, "CGATG": 4399, "CGACA": 596420, "CGACT": 425646, "CGACC": 355409, "CGACG": 194877, "CGAGA": 4488, "CGAGT": 2607, "CGAGC": 1625, "CGAGG": 1774, "CGTAA": 1144250, "CGTAT": 1049515, "CGTAC": 511899, "CGTAG": 3618, "CGTTA": 718219, "CGTTT": 638791, "CGTTC": 284526, "CGTTG": 9490, "CGTCA": 460865, "CGTCT": 387945, "CGTCC": 225902, "CGTCG": 443967, "CGTGA": 3847, "CGTGT": 333166, "CGTGC": 1939, "CGTGG": 26437, "CGCAA": 488204, "CGCAT": 330937, "CGCAC": 204388, "CGCAG": 1647, "CGCTA": 415705, "CGCTT": 297657, "CGCTC": 189749, "CGCTG": 1515, "CGCCA": 331406, "CGCCT": 156090, "CGCCC": 152608, "CGCCG": 228690, "CGCGA": 174700, "CGCGT": 98461, "CGCGC": 76282, "CGCGG": 894, "CGGAA": 27045, "CGGAT": 2633, "CGGAC": 1959, "CGGAG": 1938, "CGGTA": 2466, "CGGTT": 10997, "CGGTC": 1910, "CGGTG": 220030, "CGGCA": 1192, "CGGCT": 1202, "CGGCC": 1129, "CGGCG": 1048, "CGGGA": 6840, "CGGGT": 763, "CGGGC": 612, "CGGGG": 2799, "GAAAA": 1965864, "GAAAT": 1114542, "GAAAC": 572446, "GAAAG": 530618, "GAATA": 987864, "GAATT": 765477, "GAATC": 497909, "GAATG": 10461, "GAACA": 527294, "GAACT": 339609, "GAACC": 259030, "GAACG": 113793, "GAAGA": 519222, "GAAGT": 48756, "GAAGC": 34539, "GAAGG": 210303, "GATAA": 1052133, "GATAT": 795975, "GATAC": 439823, "GATAG": 10121, "GATTA": 688883, "GATTT": 614184, "GATTC": 311815, "GATTG": 3240, "GATCA": 513388, "GATCT": 603227, "GATCC": 223042, "GATCG": 104555, "GATGA": 11074, "GATGT": 309435, "GATGC": 2042, "GATGG": 8199, "GACAA": 512878, "GACAT": 326653, "GACAC": 196927, "GACAG": 2789, "GACTA": 341401, "GACTT": 232699, "GACTC": 127102, "GACTG": 1797, "GACCA": 244507, "GACCT": 173489, "GACCC": 133528, "GACCG": 61038, "GACGA": 105340, "GACGT": 73277, "GACGC": 41611, "GACGG": 1560, "GAGAA": 518980, "GAGAT": 304859, "GAGAC": 121266, "GAGAG": 317494, "GAGTA": 58748, "GAGTT": 44561, "GAGTC": 41038, "GAGTG": 306593, "GAGCA": 39931, "GAGCT": 33040, "GAGCC": 28872, "GAGCG": 146328, "GAGGA": 375313, "GAGGT": 40700, "GAGGC": 28425, "GAGGG": 189478, "GTAAA": 743351, "GTAAT": 446982, "GTAAC": 273968, "GTAAG": 7132, "GTATA": 520127, "GTATT": 406978, "GTATC": 358180, "GTATG": 3438, "GTACA": 289900, "GTACT": 169831, "GTACC": 119182, "GTACG": 74296, "GTAGA": 151464, "GTAGT": 282858, "GTAGC": 1575, "GTAGG": 306905, "GTTAA": 406748, "GTTAT": 354832, "GTTAC": 180999, "GTTAG": 3727, "GTTTA": 337434, "GTTTT": 327281, "GTTTC": 182635, "GTTTG": 5287, "GTTCA": 184913, "GTTCT": 129264, "GTTCC": 89233, "GTTCG": 32331, "GTTGA": 3130, "GTTGT": 27102, "GTTGC": 36389, "GTTGG": 3231, "GTCAA": 306397, "GTCAT": 251397, "GTCAC": 157954, "GTCAG": 1982, "GTCTA": 217768, "GTCTT": 145408, "GTCTC": 139897, "GTCTG": 2194, "GTCCA": 113175, "GTCCT": 89105, "GTCCC": 74929, "GTCCG": 57058, "GTCGA": 189437, "GTCGT": 392466, "GTCGC": 264317, "GTCGG": 7324, "GTGAA": 5964, "GTGAT": 308273, "GTGAC": 2826, "GTGAG": 5379, "GTGTA": 464536, "GTGTT": 9001, "GTGTC": 153910, "GTGTG": 622372, "GTGCA": 1802, "GTGCT": 8553, "GTGCC": 1437, "GTGCG": 949, "GTGGA": 5803, "GTGGT": 290417, "GTGGC": 1439, "GTGGG": 11814, "GCAAA": 337310, "GCAAT": 213683, "GCAAC": 124204, "GCAAG": 2567, "GCATA": 212772, "GCATT": 156348, "GCATC": 87571, "GCATG": 1713, "GCACA": 119228, "GCACT": 86409, "GCACC": 49344, "GCACG": 30418, "GCAGA": 3021, "GCAGT": 2046, "GCAGC": 1380, "GCAGG": 2051, "GCTAA": 238839, "GCTAT": 216194, "GCTAC": 102704, "GCTAG": 992, "GCTTA": 149079, "GCTTT": 156763, "GCTTC": 83998, "GCTTG": 1306, "GCTCA": 106752, "GCTCT": 97463, "GCTCC": 47092, "GCTCG": 29985, "GCTGA": 1777, "GCTGT": 1815, "GCTGC": 1081, "GCTGG": 1274, "GCCAA": 149586, "GCCAT": 231077, "GCCAC": 72047, "GCCAG": 1485, "GCCTA": 97770, "GCCTT": 70915, "GCCTC": 46175, "GCCTG": 1455, "GCCCA": 75786, "GCCCT": 62721, "GCCCC": 59226, "GCCCG": 27504, "GCCGA": 46232, "GCCGT": 197100, "GCCGC": 16830, "GCCGG": 840, "GCGAA": 88338, "GCGAT": 63111, "GCGAC": 35280, "GCGAG": 1621, "GCGTA": 52593, "GCGTT": 33836, "GCGTC": 328736, "GCGTG": 1911, "GCGCA": 27341, "GCGCT": 21164, "GCGCC": 20070, "GCGCG": 15217, "GCGGA": 1943, "GCGGT": 857, "GCGGC": 711, "GCGGG": 2065, "GGAAA": 725730, "GGAAT": 254355, "GGAAC": 109592, "GGAAG": 277517, "GGATA": 253217, "GGATT": 199080, "GGATC": 165165, "GGATG": 7868, "GGACA": 106082, "GGACT": 69025, "GGACC": 60884, "GGACG": 6282, "GGAGA": 358878, "GGAGT": 40131, "GGAGC": 28010, "GGAGG": 204424, "GGTAA": 49865, "GGTAT": 29253, "GGTAC": 23430, "GGTAG": 4829, "GGTTA": 41242, "GGTTT": 29837, "GGTTC": 21102, "GGTTG": 44747, "GGTCA": 49651, "GGTCT": 25235, "GGTCC": 20902, "GGTCG": 235969, "GGTGA": 4337, "GGTGT": 7118, "GGTGC": 1330, "GGTGG": 228731, "GGCAA": 34849, "GGCAT": 20186, "GGCAC": 14428, "GGCAG": 2004, "GGCTA": 31381, "GGCTT": 16972, "GGCTC": 14465, "GGCTG": 1356, "GGCCA": 25742, "GGCCT": 11797, "GGCCC": 15914, "GGCCG": 2456, "GGCGA": 3112, "GGCGT": 2113, "GGCGC": 1666, "GGCGG": 2072, "GGGAA": 561643, "GGGAT": 148881, "GGGAC": 55098, "GGGAG": 195583, "GGGTA": 27169, "GGGTT": 23724, "GGGTC": 19904, "GGGTG": 10696, "GGGCA": 17149, "GGGCT": 15169, "GGGCC": 12900, "GGGCG": 3019, "GGGGA": 211745, "GGGGT": 25577, "GGGGC": 11794, "GGGGG": 907026 }, "overrepresented_sequences": {} }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/RP5 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/RP5 (paired)_R2.fastq.gz -o output/13-wgbs/trimmed/Rio5_R1.fq.gz -O output/13-wgbs/trimmed/Rio5_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs/fastp/Rio5.fastp.json --html output/13-wgbs/fastp/Rio5.fastp.html " } }, "multiqc_general_stats": { "Pum1_pe": { "bismark-percent_cpg_meth": 19.4, "bismark-total_c": 706.674834, "bismark-C_coverage": 3.65, "bismark-dup_reads_percent": 6.99, "bismark-dedup_reads": 19.975663 }, "Pum2_pe": { "bismark-percent_cpg_meth": 19.4, "bismark-total_c": 682.454947, "bismark-C_coverage": 3.525, "bismark-dup_reads_percent": 6.76, "bismark-dedup_reads": 19.287059 }, "Pum3_pe": { "bismark-percent_cpg_meth": 19.4, "bismark-total_c": 597.033542, "bismark-C_coverage": 3.082, "bismark-dup_reads_percent": 5.9, "bismark-dedup_reads": 16.861297999999998 }, "Pum4_pe": { "bismark-percent_cpg_meth": 19.2, "bismark-total_c": 571.028422, "bismark-C_coverage": 3.021, "bismark-dup_reads_percent": 3.5, "bismark-dedup_reads": 16.368714999999998 }, "Pum5_pe": { "bismark-percent_cpg_meth": 19.4, "bismark-total_c": 425.933672, "bismark-C_coverage": 2.196, "bismark-dup_reads_percent": 4.21, "bismark-dedup_reads": 12.012233 }, "Qui1_pe": { "bismark-percent_cpg_meth": 18.9, "bismark-total_c": 681.607898, "bismark-C_coverage": 3.886, "bismark-dup_reads_percent": 7.04, "bismark-dedup_reads": 20.553335 }, "Qui2_pe": { "bismark-percent_cpg_meth": 21.6, "bismark-total_c": 652.716162, "bismark-C_coverage": 3.565, "bismark-dup_reads_percent": 6.38, "bismark-dedup_reads": 19.548927 }, "Qui3_pe": { "bismark-percent_cpg_meth": 20.5, "bismark-total_c": 740.428889, "bismark-C_coverage": 4.082, "bismark-dup_reads_percent": 7.12, "bismark-dedup_reads": 22.108613 }, "Qui4_pe": { "bismark-percent_cpg_meth": 19.3, "bismark-total_c": 611.533652, "bismark-C_coverage": 3.484, "bismark-dup_reads_percent": 5.3, "bismark-dedup_reads": 18.677678 }, "Qui5_pe": { "bismark-percent_cpg_meth": 18.9, "bismark-total_c": 589.998557, "bismark-C_coverage": 3.362, "bismark-dup_reads_percent": 6.08, "bismark-dedup_reads": 17.777774 }, "Rio1_pe": { "bismark-percent_cpg_meth": 20.3, "bismark-total_c": 788.03128, "bismark-C_coverage": 4.247, "bismark-dup_reads_percent": 6.42, "bismark-dedup_reads": 22.712055 }, "Rio2_pe": { "bismark-percent_cpg_meth": 20.1, "bismark-total_c": 662.136143, "bismark-C_coverage": 3.594, "bismark-dup_reads_percent": 6.19, "bismark-dedup_reads": 19.268973 }, "Rio3_pe": { "bismark-percent_cpg_meth": 21.1, "bismark-total_c": 574.283093, "bismark-C_coverage": 3.269, "bismark-dup_reads_percent": 4.82, "bismark-dedup_reads": 17.988678999999998 }, "Rio4_pe": { "bismark-percent_cpg_meth": 20.5, "bismark-total_c": 743.967875, "bismark-C_coverage": 4.109, "bismark-dup_reads_percent": 5.01, "bismark-dedup_reads": 22.017336999999998 }, "Rio5_pe": { "bismark-percent_cpg_meth": 21.1, "bismark-total_c": 574.283124, "bismark-C_coverage": 3.269, "bismark-dup_reads_percent": 4.82, 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