User specifified dedup report: output/13-wgbs/dedup/Pum1_pe.deduplication_report.txt A specific output filename was specified: Pum1_bismark_report.html. Using that one instead of deriving the filename Writing Bismark HTML report to >> output/13-wgbs/reports/Pum1_bismark_report.html << ============================================================================================================== Using the following alignment report: > output/13-wgbs/bismark/Pum1_PE_report.txt < Processing alignment report output/13-wgbs/bismark/Pum1_PE_report.txt ... Complete Using the following deduplication report: > output/13-wgbs/dedup/Pum1_pe.deduplication_report.txt < Processing deduplication report output/13-wgbs/dedup/Pum1_pe.deduplication_report.txt ... Complete Using the following splitting report: > output/13-wgbs/methylation/Pum1_pe.deduplicated_splitting_report.txt < Processing splitting report output/13-wgbs/methylation/Pum1_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > output/13-wgbs/methylation/Pum1_pe.deduplicated.M-bias.txt < Processing M-bias report output/13-wgbs/methylation/Pum1_pe.deduplicated.M-bias.txt ... Complete Using the following nucleotide coverage report: > output/13-wgbs/dedup/Pum1_pe.deduplicated.nucleotide_stats.txt < Processing nucleotide coverage report 'output/13-wgbs/dedup/Pum1_pe.deduplicated.nucleotide_stats.txt' ... Complete ============================================================================================================== No Bismark/Bowtie2 single-end BAM files detected Found Bismark/Bowtie2 paired-end files No Bismark/HISAT2 single-end BAM files detected No Bismark/HISAT2 paired-end BAM files detected Generating Bismark summary report from 1 Bismark BAM file(s)... >> Reading from Bismark report: Pum1_R1_bismark_bt2_PE_report.txt No deduplication report present, skipping... No methylation extractor report present, skipping... Excluding sample >Pum1_R1_bismark_bt2_pe.bam< for plotting as there were no calls in CpG context Wrote Bismark project summary to >> ../reports/bismark_summary_report.html << /// MultiQC v1.30 version_check | MultiQC Version v1.35 now available! file_search | Search path: /mmfs1/gscratch/scrubbed/sr320/github/project-ostrea-chil/output/13-wgbs/fastp file_search | Search path: /mmfs1/gscratch/scrubbed/sr320/github/project-ostrea-chil/output/13-wgbs/bismark file_search | Search path: /mmfs1/gscratch/scrubbed/sr320/github/project-ostrea-chil/output/13-wgbs/dedup file_search | Search path: /mmfs1/gscratch/scrubbed/sr320/github/project-ostrea-chil/output/13-wgbs/methylation bismark | Error calculating percentage for Pum1_R1_bismark_bt2_PE_report.txt - ignoring sample. bismark | Found 1 alignment reports bismark | Found 1 dedup reports bismark | Found 1 methextract reports bismark | Found 1 bismark bam2nuc reports fastp | Found 1 reports write_results | Data : output/13-wgbs/reports/multiqc_13-wgbs_data write_results | Report : output/13-wgbs/reports/multiqc_13-wgbs.html multiqc | MultiQC complete sample pairs_analysed mapping_pct dup_pct CpG_meth_pct CHG_meth_pct CHH_meth_pct Pum1 38376265 56.0 6.99 19.5