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"CTGAC":5842, "CTGAG":235155, "CTGTA":44678, "CTGTT":74303, "CTGTC":11507, "CTGTG":26655, "CTGCA":3759, "CTGCT":12432, "CTGCC":2947, "CTGCG":1737, "CTGGA":18210, "CTGGT":67919, "CTGGC":2112, "CTGGG":77311, "CCAAA":39881, "CCAAT":13472, "CCAAC":19675, "CCAAG":7224, "CCATA":8585, "CCATT":18285, "CCATC":11318, "CCATG":4713, "CCACA":7332, "CCACT":17722, "CCACC":13667, "CCACG":3043, "CCAGA":14613, "CCAGT":224915, "CCAGC":2567, "CCAGG":4262, "CCTAA":13627, "CCTAT":12670, "CCTAC":8253, "CCTAG":4888, "CCTTA":14806, "CCTTT":187185, "CCTTC":231299, "CCTTG":10739, "CCTCA":22609, "CCTCT":270941, "CCTCC":391058, "CCTCG":2689, "CCTGA":7006, "CCTGT":9576, "CCTGC":2631, "CCTGG":4077, "CCCAA":29011, "CCCAT":12889, "CCCAC":16516, "CCCAG":15736, "CCCTA":10441, "CCCTT":143836, "CCCTC":276852, "CCCTG":4853, "CCCCA":30050, "CCCCT":169472, "CCCCC":289216, "CCCCG":3268, "CCCGA":3931, "CCCGT":4402, "CCCGC":1464, "CCCGG":1706, "CCGAA":6897, "CCGAT":7579, "CCGAC":2098, "CCGAG":2724, "CCGTA":4158, "CCGTT":14801, "CCGTC":13241, "CCGTG":3678, "CCGCA":1143, "CCGCT":1892, "CCGCC":1390, "CCGCG":995, "CCGGA":3132, "CCGGT":4177, "CCGGC":1403, "CCGGG":2338, "CGAAA":448190, "CGAAT":447155, "CGAAC":23622, "CGAAG":208800, "CGATA":600259, "CGATT":708723, "CGATC":55756, "CGATG":254189, "CGACA":3280, "CGACT":4948, "CGACC":2492, "CGACG":87952, "CGAGA":283901, "CGAGT":329371, "CGAGC":19032, "CGAGG":146657, "CGTAA":528826, "CGTAT":714838, "CGTAC":48930, "CGTAG":254131, "CGTTA":772258, "CGTTT":1139301, "CGTTC":80408, "CGTTG":301763, "CGTCA":8686, "CGTCT":284058, "CGTCC":8630, "CGTCG":126096, "CGTGA":235115, "CGTGT":408717, "CGTGC":20661, "CGTGG":136802, "CGCAA":3127, "CGCAT":3907, "CGCAC":1667, "CGCAG":1665, "CGCTA":3235, "CGCTT":6814, "CGCTC":3400, "CGCTG":1810, "CGCCA":938, "CGCCT":2742, "CGCCC":2717, "CGCCG":901, "CGCGA":70119, "CGCGT":97004, "CGCGC":10415, "CGCGG":48425, "CGGAA":279221, "CGGAT":376674, "CGGAC":38906, "CGGAG":141684, "CGGTA":329437, "CGGTT":462676, "CGGTC":38448, "CGGTG":174836, 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"GAGTC":75252, "GAGTG":3215528, "GAGCA":96772, "GAGCT":17220, "GAGCC":8136, "GAGCG":123966, "GAGGA":2334794, "GAGGT":2704525, "GAGGC":30028, "GAGGG":1367093, "GTAAA":11356117, "GTAAT":12001286, "GTAAC":116852, "GTAAG":4390413, "GTATA":15279384, "GTATT":18419721, "GTATC":240987, "GTATG":7514966, "GTACA":34234, "GTACT":259688, "GTACC":13476, "GTACG":327591, "GTAGA":5343005, "GTAGT":6388149, "GTAGC":63615, "GTAGG":2772237, "GTTAA":11650136, "GTTAT":17843161, "GTTAC":181464, "GTTAG":5995233, "GTTTA":16599911, "GTTTT":29057134, "GTTTC":423275, "GTTTG":10303676, "GTTCA":69235, "GTTCT":148558, "GTTCC":80009, "GTTCG":506248, "GTTGA":6865685, "GTTGT":9714144, "GTTGC":81577, "GTTGG":3461865, "GTCAA":51849, "GTCAT":62257, "GTCAC":227504, "GTCAG":23015, "GTCTA":48512, "GTCTT":165436, "GTCTC":72750, "GTCTG":247652, "GTCCA":5325, "GTCCT":66590, "GTCCC":79412, "GTCCG":3926, "GTCGA":320628, "GTCGT":468593, "GTCGC":23022, "GTCGG":246357, "GTGAA":5601742, "GTGAT":7405742, "GTGAC":91352, "GTGAG":2649950, "GTGTA":8167719, "GTGTT":10083701, "GTGTC":164951, "GTGTG":5761411, "GTGCA":18385, "GTGCT":26305, "GTGCC":17793, "GTGCG":133564, "GTGGA":2814684, "GTGGT":3850457, "GTGGC":71891, "GTGGG":1792773, "GCAAA":27539, "GCAAT":27010, "GCAAC":3915, "GCAAG":10844, "GCATA":29061, "GCATT":40815, "GCATC":5916, "GCATG":16802, "GCACA":228842, "GCACT":3823, "GCACC":3040, "GCACG":1848, "GCAGA":12466, "GCAGT":17623, "GCAGC":2672, "GCAGG":7716, "GCTAA":20778, "GCTAT":31595, "GCTAC":3016, "GCTAG":10994, "GCTTA":24660, "GCTTT":85984, "GCTTC":24852, "GCTTG":17261, "GCTCA":3250, "GCTCT":23623, "GCTCC":27962, "GCTCG":2334, "GCTGA":11674, "GCTGT":18442, "GCTGC":1815, "GCTGG":6707, "GCCAA":3168, "GCCAT":4070, "GCCAC":2154, "GCCAG":1706, "GCCTA":2571, "GCCTT":18793, "GCCTC":26257, "GCCTG":2162, "GCCCA":2332, "GCCCT":21223, "GCCCC":30892, "GCCCG":779, "GCCGA":1447, "GCCGT":14632, "GCCGC":697, "GCCGG":2221, "GCGAA":186560, "GCGAT":287258, "GCGAC":13978, "GCGAG":150032, "GCGTA":269811, "GCGTT":389177, "GCGTC":50777, 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"GGGTC":87946, "GGGTG":1835551, "GGGCA":11895, "GGGCT":13333, "GGGCC":9195, "GGGCG":146535, "GGGGA":1579399, "GGGGT":2494266, "GGGGC":87287, "GGGGG":1974687 }, "overrepresented_sequences": { } }, "read2_after_filtering": { "total_reads": 22411610, "total_bases": 3103986879, "q20_bases": 3075020329, "q30_bases": 2986593824, "q40_bases": 2986593824, "total_cycles": 141, "quality_curves": { 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"CTGAG":9179, "CTGTA":19058, "CTGTT":14888, "CTGTC":9351, "CTGTG":2304, "CTGCA":9077, "CTGCT":6385, "CTGCC":4268, "CTGCG":973, "CTGGA":14045, "CTGGT":744, "CTGGC":670, "CTGGG":31793, "CCAAA":10967515, "CCAAT":6490437, "CCAAC":3631742, "CCAAG":10171, "CCATA":6797644, "CCATT":5022826, "CCATC":2673356, "CCATG":13679, "CCACA":3928460, "CCACT":2476964, "CCACC":2183542, "CCACG":136639, "CCAGA":15297, "CCAGT":7774, "CCAGC":4303, "CCAGG":1038, "CCTAA":6116234, "CCTAT":5120276, "CCTAC":2744161, "CCTAG":7119, "CCTTA":4414723, "CCTTT":4172209, "CCTTC":1981432, "CCTTG":7168, "CCTCA":2431297, "CCTCT":2270647, "CCTCC":1401331, "CCTCG":140233, "CCTGA":11366, "CCTGT":8054, "CCTGC":4215, "CCTGG":1064, "CCCAA":4222462, "CCCAT":2600957, "CCCAC":1887834, "CCCAG":9845, "CCCTA":3081402, "CCCTT":2040772, "CCCTC":1377970, "CCCTG":6376, "CCCCA":2213669, "CCCCT":1687479, "CCCCC":3139842, "CCCCG":129303, "CCCGA":266376, "CCCGT":129289, "CCCGC":97207, "CCCGG":1222, "CCGAA":601046, "CCGAT":426075, "CCGAC":245115, "CCGAG":1220, "CCGTA":537938, "CCGTT":210870, "CCGTC":172940, "CCGTG":1713, "CCGCA":122153, "CCGCT":120691, "CCGCC":107679, "CCGCG":47832, "CCGGA":1618, "CCGGT":990, "CCGGC":653, "CCGGG":546, "CGAAA":1407909, "CGAAT":790101, "CGAAC":501766, "CGAAG":3433, "CGATA":850296, "CGATT":530338, "CGATC":399986, "CGATG":2155, "CGACA":376737, "CGACT":276041, "CGACC":217340, "CGACG":124886, "CGAGA":2514, "CGAGT":1462, "CGAGC":1014, "CGAGG":1175, "CGTAA":741188, "CGTAT":774012, "CGTAC":321246, "CGTAG":1669, "CGTTA":452303, "CGTTT":394719, "CGTTC":176684, "CGTTG":1736, "CGTCA":306031, "CGTCT":224599, "CGTCC":139721, "CGTCG":303490, "CGTGA":2125, "CGTGT":221939, "CGTGC":1162, "CGTGG":1558, "CGCAA":311097, "CGCAT":209807, "CGCAC":128933, "CGCAG":1374, "CGCTA":263541, "CGCTT":192653, "CGCTC":118131, "CGCTG":808, "CGCCA":162990, "CGCCT":101086, "CGCCC":93228, "CGCCG":255167, "CGCGA":110195, "CGCGT":61921, "CGCGC":48392, "CGCGG":521, "CGGAA":16873, "CGGAT":1367, "CGGAC":1088, "CGGAG":1264, "CGGTA":1328, "CGGTT":1641, "CGGTC":867, "CGGTG":216017, "CGGCA":813, "CGGCT":707, "CGGCC":756, "CGGCG":697, "CGGGA":3956, "CGGGT":431, "CGGGC":430, "CGGGG":1617, "GAAAA":1405958, "GAAAT":767927, "GAAAC":400078, "GAAAG":417205, "GAATA":713717, "GAATT":521812, "GAATC":298893, "GAATG":10048, "GAACA":367188, "GAACT":233606, "GAACC":166525, "GAACG":75534, "GAAGA":414676, "GAAGT":32291, "GAAGC":27229, "GAAGG":199680, "GATAA":724030, "GATAT":557731, "GATAC":289801, "GATAG":12055, "GATTA":449735, "GATTT":425006, "GATTC":193272, "GATTG":1632, "GATCA":267229, "GATCT":454750, "GATCC":112976, "GATCG":63868, "GATGA":12011, "GATGT":2470, "GATGC":1434, "GATGG":10408, "GACAA":563289, "GACAT":219675, "GACAC":127794, "GACAG":1791, "GACTA":238643, "GACTT":167149, "GACTC":84423, "GACTG":976, "GACCA":151683, "GACCT":93130, "GACCC":79145, "GACCG":37235, "GACGA":67945, "GACGT":45799, "GACGC":27473, "GACGG":1103, "GAGAA":464980, "GAGAT":226669, "GAGAC":95002, "GAGAG":299254, "GAGTA":44972, "GAGTT":31268, "GAGTC":17827, "GAGTG":217155, "GAGCA":35625, "GAGCT":27490, "GAGCC":16132, "GAGCG":80921, "GAGGA":346277, "GAGGT":28486, "GAGGC":22928, "GAGGG":187264, "GTAAA":472548, "GTAAT":279886, "GTAAC":174570, "GTAAG":5142, "GTATA":328378, "GTATT":253175, "GTATC":340993, "GTATG":2002, "GTACA":180347, "GTACT":104628, "GTACC":73053, "GTACG":45418, "GTAGA":202807, "GTAGT":2174, "GTAGC":999, "GTAGG":218911, "GTTAA":250036, "GTTAT":213195, "GTTAC":114241, "GTTAG":1476, "GTTTA":216933, "GTTTT":196263, "GTTTC":107280, "GTTTG":2459, "GTTCA":110768, "GTTCT":75124, "GTTCC":53495, "GTTCG":19582, "GTTGA":1711, "GTTGT":3505, "GTTGC":6149, "GTTGG":1764, "GTCAA":162965, "GTCAT":152609, "GTCAC":92689, "GTCAG":1150, "GTCTA":131214, "GTCTT":86565, "GTCTC":63501, "GTCTG":1238, "GTCCA":62703, "GTCCT":48443, "GTCCC":41793, "GTCCG":34153, "GTCGA":58057, "GTCGT":256808, "GTCGC":226484, "GTCGG":4032, "GTGAA":6179, "GTGAT":2202, "GTGAC":217950, "GTGAG":4453, "GTGTA":410014, "GTGTT":4370, "GTGTC":23211, "GTGTG":222156, "GTGCA":1171, "GTGCT":6407, "GTGCC":1082, "GTGCG":569, "GTGGA":5125, "GTGGT":221452, "GTGGC":1220, "GTGGG":8225, "GCAAA":226856, "GCAAT":140798, "GCAAC":80845, "GCAAG":1584, "GCATA":142556, "GCATT":101799, "GCATC":58950, "GCATG":986, "GCACA":76189, "GCACT":54252, "GCACC":32848, "GCACG":20213, "GCAGA":1985, "GCAGT":1613, "GCAGC":952, "GCAGG":1295, "GCTAA":160556, "GCTAT":135627, "GCTAC":68366, "GCTAG":572, "GCTTA":101432, "GCTTT":98546, "GCTTC":61638, "GCTTG":714, "GCTCA":66358, "GCTCT":60408, "GCTCC":29806, "GCTCG":18901, "GCTGA":1009, "GCTGT":794, "GCTGC":661, "GCTGG":784, "GCCAA":92612, "GCCAT":86619, "GCCAC":43841, "GCCAG":865, "GCCTA":64316, "GCCTT":44349, "GCCTC":29254, "GCCTG":843, "GCCCA":43816, "GCCCT":33241, "GCCCC":36231, "GCCCG":16894, "GCCGA":28866, "GCCGT":224946, "GCCGC":10535, "GCCGG":517, "GCGAA":55906, "GCGAT":39798, "GCGAC":22702, "GCGAG":1069, "GCGTA":33427, "GCGTT":20819, "GCGTC":231846, "GCGTG":1256, "GCGCA":17649, "GCGCT":13559, "GCGCC":13122, "GCGCG":9293, "GCGGA":1247, "GCGGT":450, "GCGGC":508, "GCGGG":1302, "GGAAA":599152, "GGAAT":208872, "GGAAC":89751, "GGAAG":233983, "GGATA":212486, "GGATT":155179, "GGATC":82404, "GGATG":10115, "GGACA":86695, "GGACT":59962, "GGACC":37580, "GGACG":4527, "GGAGA":330048, "GGAGT":28440, "GGAGC":22868, "GGAGG":200466, "GGTAA":37621, "GGTAT":25457, "GGTAC":15597, "GGTAG":4723, "GGTTA":25795, "GGTTT":21737, "GGTTC":11796, "GGTTG":7482, "GGTCA":18208, "GGTCT":9446, "GGTCC":7046, "GGTCG":214348, "GGTGA":4425, "GGTGT":2294, "GGTGC":935, "GGTGG":223127, "GGCAA":31029, "GGCAT":19469, "GGCAC":10982, "GGCAG":1367, "GGCTA":25865, "GGCTT":16069, "GGCTC":9643, "GGCTG":768, "GGCCA":14243, "GGCCT":8236, "GGCCC":7585, "GGCCG":1277, "GGCGA":2398, "GGCGT":1428, "GGCGC":1093, "GGCGG":1396, "GGGAA":458888, "GGGAT":118141, "GGGAC":45930, "GGGAG":190233, "GGGTA":22625, "GGGTT":16749, "GGGTC":9022, "GGGTG":7814, "GGGCA":15711, "GGGCT":13298, "GGGCC":7739, "GGGCG":2018, "GGGGA":200228, "GGGGT":18582, "GGGGC":10146, "GGGGG":731697 }, "overrepresented_sequences": { } }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/PUM5 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/PUM5 (paired)_R2.fastq.gz -o output/13-wgbs/trimmed/Pum5_R1.fq.gz -O output/13-wgbs/trimmed/Pum5_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs/fastp/Pum5.fastp.json --html output/13-wgbs/fastp/Pum5.fastp.html " }