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"TCCGA":14990, "TCCGT":17397, "TCCGC":4250, "TCCGG":6915, "TCGAA":750967, "TCGAT":1113169, "TCGAC":57068, "TCGAG":559159, "TCGTA":985845, "TCGTT":1574391, "TCGTC":127668, "TCGTG":562440, "TCGCA":6094, "TCGCT":9178, "TCGCC":4267, "TCGCG":151824, "TCGGA":556338, "TCGGT":725719, "TCGGC":40345, "TCGGG":384717, "TGAAA":18747452, "TGAAT":16865242, "TGAAC":446595, "TGAAG":7092027, "TGATA":18741611, "TGATT":23531272, "TGATC":295736, "TGATG":10228681, "TGACA":44825, "TGACT":66627, "TGACC":26592, "TGACG":409534, "TGAGA":6427506, "TGAGT":7639022, "TGAGC":84813, "TGAGG":3336301, "TGTAA":18970065, "TGTAT":29833160, "TGTAC":255942, "TGTAG":9738561, "TGTTA":23713623, "TGTTT":38704055, "TGTTC":485447, "TGTTG":13867911, "TGTCA":181371, "TGTCT":199898, "TGTCC":114022, "TGTCG":578515, "TGTGA":9720026, "TGTGT":16617316, "TGTGC":122092, "TGTGG":5215943, "TGCAA":47846, "TGCAT":64668, "TGCAC":5801, "TGCAG":25897, "TGCTA":46618, "TGCTT":113664, "TGCTC":38510, "TGCTG":28494, "TGCCA":7935, "TGCCT":32650, 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"CACCC":22820, "CACCG":3141, "CACGA":6925, "CACGT":311306, "CACGC":2931, "CACGG":3872, "CAGAA":49643, "CAGAT":61661, "CAGAC":7124, "CAGAG":24762, "CAGTA":47454, "CAGTT":85036, "CAGTC":306220, "CAGTG":33947, "CAGCA":5466, "CAGCT":7071, "CAGCC":4487, "CAGCG":2486, "CAGGA":22411, "CAGGT":33928, "CAGGC":3442, "CAGGG":18510, "CTAAA":108388, "CTAAT":94346, "CTAAC":16806, "CTAAG":32485, "CTATA":121579, "CTATT":152169, "CTATC":312791, "CTATG":57508, "CTACA":11830, "CTACT":21141, "CTACC":10712, "CTACG":5213, "CTAGA":43149, "CTAGT":133893, "CTAGC":3959, "CTAGG":87590, "CTTAA":108019, "CTTAT":127692, "CTTAC":19059, "CTTAG":44408, "CTTTA":159722, "CTTTT":774847, "CTTTC":348114, "CTTTG":114092, "CTTCA":58271, "CTTCT":400618, "CTTCC":508451, "CTTCG":13887, "CTTGA":65291, "CTTGT":101007, "CTTGC":9275, "CTTGG":38221, "CTCAA":46489, "CTCAT":26929, "CTCAC":22132, "CTCAG":17927, "CTCTA":29481, "CTCTT":363349, "CTCTC":600994, "CTCTG":14932, "CTCCA":310972, "CTCCT":442489, "CTCCC":698717, "CTCCG":4857, "CTCGA":9823, "CTCGT":13922, "CTCGC":3211, "CTCGG":5326, "CTGAA":352321, "CTGAT":51670, "CTGAC":8508, "CTGAG":241381, "CTGTA":62129, "CTGTT":104262, "CTGTC":92836, "CTGTG":37611, "CTGCA":5799, "CTGCT":18571, "CTGCC":4833, "CTGCG":2422, "CTGGA":25678, "CTGGT":99721, "CTGGC":3446, "CTGGG":102555, "CCAAA":54738, "CCAAT":18509, "CCAAC":27282, "CCAAG":10127, "CCATA":11721, "CCATT":26253, "CCATC":16396, "CCATG":6922, "CCACA":10415, "CCACT":24599, "CCACC":19519, "CCACG":4223, "CCAGA":21688, "CCAGT":303748, "CCAGC":4335, "CCAGG":6360, "CCTAA":18889, "CCTAT":17390, "CCTAC":11305, "CCTAG":7158, "CCTTA":20374, "CCTTT":271427, "CCTTC":348442, "CCTTG":15048, "CCTCA":31806, "CCTCT":411075, "CCTCC":595178, "CCTCG":3726, "CCTGA":10117, "CCTGT":90270, "CCTGC":4154, "CCTGG":6326, "CCCAA":40496, "CCCAT":18188, "CCCAC":22903, "CCCAG":23078, "CCCTA":14528, "CCCTT":213278, "CCCTC":419881, "CCCTG":7219, "CCCCA":42837, "CCCCT":254964, "CCCCC":435755, "CCCCG":4591, "CCCGA":5525, "CCCGT":6038, "CCCGC":2106, "CCCGG":2450, "CCGAA":9627, "CCGAT":10698, "CCGAC":2864, "CCGAG":3713, "CCGTA":5636, "CCGTT":20643, "CCGTC":20128, "CCGTG":5122, "CCGCA":1573, "CCGCT":2680, "CCGCC":2138, "CCGCG":1462, "CCGGA":4427, "CCGGT":5853, "CCGGC":2096, "CCGGG":3367, "CGAAA":599983, "CGAAT":598985, "CGAAC":31759, "CGAAG":279119, "CGATA":803662, "CGATT":956571, "CGATC":76206, "CGATG":342495, "CGACA":4503, "CGACT":6955, "CGACC":3658, "CGACG":118868, "CGAGA":378758, "CGAGT":445255, "CGAGC":25987, "CGAGG":197871, "CGTAA":707182, "CGTAT":956596, "CGTAC":66260, "CGTAG":342193, "CGTTA":1038282, "CGTTT":1548264, "CGTTC":110711, "CGTTG":410046, "CGTCA":12060, "CGTCT":388521, "CGTCC":12440, "CGTCG":172551, "CGTGA":316555, "CGTGT":554768, "CGTGC":28258, "CGTGG":187027, "CGCAA":4299, "CGCAT":5344, "CGCAC":2326, "CGCAG":2375, "CGCTA":4312, "CGCTT":9368, "CGCTC":4750, "CGCTG":2592, "CGCCA":1438, "CGCCT":4024, "CGCCC":4027, "CGCCG":1296, "CGCGA":94799, "CGCGT":131232, "CGCGC":14153, "CGCGG":65841, "CGGAA":372779, "CGGAT":508279, "CGGAC":52483, "CGGAG":191363, "CGGTA":442401, "CGGTT":628084, "CGGTC":53215, "CGGTG":236392, "CGGCA":2143, "CGGCT":15442, "CGGCC":2533, "CGGCG":68392, "CGGGA":196949, "CGGGT":297176, "CGGGC":23414, "CGGGG":187574, "GAAAA":15738014, "GAAAT":17667401, "GAAAC":156415, "GAAAG":6209975, "GAATA":12682384, "GAATT":16773685, "GAATC":214359, "GAATG":6397404, "GAACA":37576, "GAACT":350214, "GAACC":20894, "GAACG":238628, "GAAGA":6002231, "GAAGT":6903204, "GAAGC":74554, "GAAGG":2694967, "GATAA":13122832, "GATAT":19832532, "GATAC":184013, "GATAG":7189625, "GATTA":13927001, "GATTT":24733566, "GATTC":364695, "GATTG":8664790, "GATCA":47342, "GATCT":124476, "GATCC":95062, "GATCG":447226, "GATGA":6706259, "GATGT":10029131, "GATGC":77302, "GATGG":3626883, "GACAA":33697, "GACAT":41887, "GACAC":4642, "GACAG":18635, "GACTA":27018, "GACTT":63783, "GACTC":25979, "GACTG":17714, "GACCA":8159, "GACCT":18420, "GACCC":24478, "GACCG":2306, "GACGA":309661, "GACGT":466943, "GACGC":17483, "GACGG":239393, "GAGAA":5869777, "GAGAT":6621962, "GAGAC":76124, "GAGAG":6966457, "GAGTA":5660440, "GAGTT":7781605, "GAGTC":105385, "GAGTG":4268875, "GAGCA":130930, "GAGCT":24505, "GAGCC":12690, "GAGCG":167317, "GAGGA":3118295, "GAGGT":3662767, "GAGGC":41678, "GAGGG":1836236, "GTAAA":15157381, "GTAAT":16068433, "GTAAC":157503, "GTAAG":5877971, "GTATA":20402103, "GTATT":24859342, "GTATC":334696, "GTATG":10121425, "GTACA":48075, "GTACT":276321, "GTACC":20242, "GTACG":440702, "GTAGA":7149959, "GTAGT":8630947, "GTAGC":87405, "GTAGG":3747310, "GTTAA":15647398, "GTTAT":24079093, "GTTAC":248923, "GTTAG":8119074, "GTTTA":22416165, "GTTTT":39441255, "GTTTC":591364, "GTTTG":14020103, "GTTCA":98015, "GTTCT":213501, "GTTCC":122750, "GTTCG":692006, "GTTGA":9292876, "GTTGT":13243971, "GTTGC":112688, "GTTGG":4720100, "GTCAA":72462, "GTCAT":89634, "GTCAC":383512, "GTCAG":32755, "GTCTA":66431, "GTCTT":238149, "GTCTC":111683, "GTCTG":335135, "GTCCA":7708, "GTCCT":101608, "GTCCC":123247, "GTCCG":5428, "GTCGA":434433, "GTCGT":640822, "GTCGC":31386, "GTCGG":333298, "GTGAA":7485244, "GTGAT":9998408, "GTGAC":124438, "GTGAG":3558784, "GTGTA":10978419, "GTGTT":13695952, "GTGTC":230828, "GTGTG":7758343, "GTGCA":26045, "GTGCT":37227, "GTGCC":26833, "GTGCG":179957, "GTGGA":3797435, "GTGGT":5272764, "GTGGC":99987, "GTGGG":2441547, "GCAAA":38633, "GCAAT":38383, "GCAAC":5548, "GCAAG":15296, "GCATA":40827, "GCATT":58434, "GCATC":8372, "GCATG":24220, "GCACA":309366, "GCACT":5598, "GCACC":4532, "GCACG":2582, "GCAGA":18076, "GCAGT":25075, "GCAGC":3969, "GCAGG":11322, "GCTAA":28923, "GCTAT":43764, "GCTAC":4272, "GCTAG":15153, "GCTTA":34333, "GCTTT":120348, "GCTTC":36676, "GCTTG":24464, "GCTCA":4857, "GCTCT":35592, "GCTCC":42791, "GCTCG":3167, "GCTGA":16771, "GCTGT":25981, "GCTGC":2919, "GCTGG":9872, "GCCAA":4715, "GCCAT":6344, "GCCAC":3427, "GCCAG":2857, "GCCTA":3738, "GCCTT":28062, "GCCTC":40646, "GCCTG":3586, "GCCCA":3717, "GCCCT":32595, "GCCCC":47444, "GCCCG":1158, "GCCGA":2013, "GCCGT":22373, "GCCGC":1101, "GCCGG":3248, "GCGAA":248921, "GCGAT":386493, "GCGAC":19124, "GCGAG":201483, "GCGTA":362094, "GCGTT":526897, "GCGTC":70322, "GCGTG":181008, "GCGCA":2965, "GCGCT":3870, "GCGCC":1973, "GCGCG":69399, "GCGGA":169366, "GCGGT":239500, "GCGGC":23487, "GCGGG":137416, "GGAAA":7027244, "GGAAT":6521210, "GGAAC":63435, "GGAAG":3145804, "GGATA":6629248, "GGATT":8453039, "GGATC":130548, "GGATG":3574595, "GGACA":18527, "GGACT":25840, "GGACC":10751, "GGACG":198224, "GGAGA":3212468, "GGAGT":3349249, "GGAGC":36904, "GGAGG":1943675, "GGTAA":6441459, "GGTAT":9287989, "GGTAC":99028, "GGTAG":3570644, "GGTTA":9435409, "GGTTT":13483856, "GGTTC":237656, "GGTTG":4897210, "GGTCA":41709, "GGTCT":70557, "GGTCC":49488, "GGTCG":316921, "GGTGA":3945305, "GGTGT":5649640, "GGTGC":46196, "GGTGG":2849146, "GGCAA":16518, "GGCAT":26002, "GGCAC":2628, "GGCAG":13561, "GGCTA":16383, "GGCTT":49219, "GGCTC":18074, "GGCTG":10741, "GGCCA":3752, "GGCCT":17310, "GGCCC":19025, "GGCCG":6900, "GGCGA":176013, "GGCGT":288242, "GGCGC":18275, "GGCGG":175498, "GGGAA":3189170, "GGGAT":3897589, "GGGAC":53985, "GGGAG":1808257, "GGGTA":3733504, "GGGTT":6156099, "GGGTC":122154, "GGGTG":2497910, "GGGCA":16500, "GGGCT":19733, "GGGCC":14416, "GGGCG":195677, "GGGGA":2119794, "GGGGT":3393885, "GGGGC":114777, "GGGGG":2635413 }, "overrepresented_sequences": { } }, "read2_after_filtering": { "total_reads": 30156298, "total_bases": 4172169560, "q20_bases": 4134068418, "q30_bases": 4018297310, "q40_bases": 4018297310, "total_cycles": 141, "quality_curves": { 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"CGCGG":840, "CGGAA":23579, "CGGAT":2075, "CGGAC":1608, "CGGAG":1821, "CGGTA":1884, "CGGTT":2328, "CGGTC":1325, "CGGTG":291020, "CGGCA":1197, "CGGCT":1075, "CGGCC":1202, "CGGCG":1030, "CGGGA":5697, "CGGGT":695, "CGGGC":726, "CGGGG":2409, "GAAAA":1892455, "GAAAT":1034086, "GAAAC":544005, "GAAAG":552823, "GAATA":953046, "GAATT":695094, "GAATC":408995, "GAATG":14541, "GAACA":499438, "GAACT":315645, "GAACC":231427, "GAACG":104200, "GAAGA":540042, "GAAGT":43345, "GAAGC":36200, "GAAGG":259384, "GATAA":973830, "GATAT":742022, "GATAC":390263, "GATAG":16151, "GATTA":602930, "GATTT":560090, "GATTC":260454, "GATTG":2837, "GATCA":370857, "GATCT":613326, "GATCC":156550, "GATCG":87051, "GATGA":16545, "GATGT":4009, "GATGC":2333, "GATGG":14042, "GACAA":690576, "GACAT":297793, "GACAC":175562, "GACAG":3066, "GACTA":320041, "GACTT":221392, "GACTC":115013, "GACTG":1927, "GACCA":211472, "GACCT":129168, "GACCC":109577, "GACCG":51518, "GACGA":93477, "GACGT":62502, "GACGC":38123, "GACGG":1651, "GAGAA":603592, "GAGAT":296898, "GAGAC":127064, "GAGAG":387226, "GAGTA":58984, "GAGTT":41648, "GAGTC":25289, "GAGTG":292811, "GAGCA":46633, "GAGCT":35839, "GAGCC":23366, "GAGCG":108034, "GAGGA":449470, "GAGGT":37941, "GAGGC":30584, "GAGGG":244180, "GTAAA":640376, "GTAAT":445352, "GTAAC":239510, "GTAAG":7251, "GTATA":443709, "GTATT":338729, "GTATC":458697, "GTATG":3067, "GTACA":244693, "GTACT":142094, "GTACC":99420, "GTACG":62036, "GTAGA":271055, "GTAGT":3257, "GTAGC":1670, "GTAGG":294971, "GTTAA":339189, "GTTAT":287766, "GTTAC":152703, "GTTAG":2363, "GTTTA":290050, "GTTTT":263421, "GTTTC":143936, "GTTTG":3947, "GTTCA":150025, "GTTCT":101994, "GTTCC":72168, "GTTCG":26367, "GTTGA":2881, "GTTGT":5406, "GTTGC":8566, "GTTGG":2869, "GTCAA":224003, "GTCAT":212202, "GTCAC":125763, "GTCAG":2056, "GTCTA":178648, "GTCTT":117334, "GTCTC":86423, "GTCTG":2207, "GTCCA":86609, "GTCCT":66202, "GTCCC":56905, "GTCCG":46950, "GTCGA":81384, "GTCGT":347240, "GTCGC":305493, "GTCGG":5683, "GTGAA":8555, "GTGAT":3646, "GTGAC":218872, "GTGAG":6591, "GTGTA":616441, "GTGTT":6159, "GTGTC":42414, "GTGTG":224515, "GTGCA":2127, "GTGCT":9077, "GTGCC":1773, "GTGCG":861, "GTGGA":7344, "GTGGT":298884, "GTGGC":2043, "GTGGG":11540, "GCAAA":307599, "GCAAT":189989, "GCAAC":110345, "GCAAG":2663, "GCATA":191112, "GCATT":136577, "GCATC":79133, "GCATG":1885, "GCACA":103771, "GCACT":73514, "GCACC":45062, "GCACG":27762, "GCAGA":3405, "GCAGT":2656, "GCAGC":1774, "GCAGG":2443, "GCTAA":216600, "GCTAT":183169, "GCTAC":92787, "GCTAG":1043, "GCTTA":135071, "GCTTT":132068, "GCTTC":80504, "GCTTG":1482, "GCTCA":91408, "GCTCT":82094, "GCTCC":40337, "GCTCG":25964, "GCTGA":2141, "GCTGT":1685, "GCTGC":1385, "GCTGG":1911, "GCCAA":128237, "GCCAT":120083, "GCCAC":61170, "GCCAG":1804, "GCCTA":87183, "GCCTT":60549, "GCCTC":40661, "GCCTG":1844, "GCCCA":61782, "GCCCT":46294, "GCCCC":50263, "GCCCG":23431, "GCCGA":40079, "GCCGT":303022, "GCCGC":14476, "GCCGG":841, "GCGAA":76397, "GCGAT":53685, "GCGAC":30999, "GCGAG":1556, "GCGTA":45146, "GCGTT":27907, "GCGTC":312716, "GCGTG":1740, "GCGCA":23646, "GCGCT":18741, "GCGCC":18054, "GCGCG":12795, "GCGGA":1814, "GCGGT":727, "GCGGC":847, "GCGGG":1965, "GGAAA":789894, "GGAAT":271403, "GGAAC":119968, "GGAAG":304796, "GGATA":276501, "GGATT":201842, "GGATC":113693, "GGATG":13707, "GGACA":115680, "GGACT":78364, "GGACC":53195, "GGACG":6092, "GGAGA":428832, "GGAGT":37777, "GGAGC":30331, "GGAGG":261857, "GGTAA":49707, "GGTAT":32843, "GGTAC":21082, "GGTAG":6538, "GGTTA":34570, "GGTTT":28297, "GGTTC":16019, "GGTTG":10488, "GGTCA":26246, "GGTCT":13152, "GGTCC":10275, "GGTCG":288949, "GGTGA":6435, "GGTGT":3753, "GGTGC":1646, "GGTGG":300909, "GGCAA":40825, "GGCAT":25176, "GGCAC":14695, "GGCAG":2592, "GGCTA":33808, "GGCTT":20826, "GGCTC":13263, "GGCTG":1890, "GGCCA":20775, "GGCCT":11783, "GGCCC":11195, "GGCCG":1977, "GGCGA":3301, "GGCGT":2011, "GGCGC":1564, "GGCGG":2109, "GGGAA":607686, "GGGAT":154091, "GGGAC":61406, "GGGAG":249001, "GGGTA":29850, "GGGTT":22138, "GGGTC":12774, "GGGTG":10877, "GGGCA":20846, "GGGCT":17625, "GGGCC":11176, "GGGCG":2883, "GGGGA":262798, "GGGGT":24943, "GGGGC":13829, "GGGGG":991550 }, "overrepresented_sequences": { } }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/PUM4 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/PUM4 (paired)_R2.fastq.gz -o output/13-wgbs/trimmed/Pum4_R1.fq.gz -O output/13-wgbs/trimmed/Pum4_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs/fastp/Pum4.fastp.json --html output/13-wgbs/fastp/Pum4.fastp.html " }