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"TCCCC":930960, "TCCCG":8092, "TCCGA":18137, "TCCGT":21528, "TCCGC":5007, "TCCGG":8532, "TCGAA":955105, "TCGAT":1413363, "TCGAC":71882, "TCGAG":708683, "TCGTA":1255171, "TCGTT":1982104, "TCGTC":156239, "TCGTG":706878, "TCGCA":7644, "TCGCT":11363, "TCGCC":5014, "TCGCG":191526, "TCGGA":706087, "TCGGT":912483, "TCGGC":50093, "TCGGG":482324, "TGAAA":24117986, "TGAAT":21504462, "TGAAC":563305, "TGAAG":9062701, "TGATA":23944706, "TGATT":29770646, "TGATC":351412, "TGATG":12993264, "TGACA":54262, "TGACT":78335, "TGACC":28525, "TGACG":523168, "TGAGA":8239318, "TGAGT":9793055, "TGAGC":104196, "TGAGG":4217806, "TGTAA":24243161, "TGTAT":37974162, "TGTAC":320932, "TGTAG":12345713, "TGTTA":30107592, "TGTTT":48835570, "TGTTC":590224, "TGTTG":17426664, "TGTCA":126263, "TGTCT":238568, "TGTCC":128191, "TGTCG":723267, "TGTGA":12344269, "TGTGT":21061138, "TGTGC":150377, "TGTGG":6549117, "TGCAA":58038, "TGCAT":77737, "TGCAC":6718, "TGCAG":30508, "TGCTA":57496, "TGCTT":137443, "TGCTC":44570, "TGCTG":34422, 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"CACCA":26873, "CACCT":14722, "CACCC":27398, "CACCG":3771, "CACGA":8835, "CACGT":394067, "CACGC":3418, "CACGG":4784, "CAGAA":59679, "CAGAT":73646, "CAGAC":8388, "CAGAG":29339, "CAGTA":56688, "CAGTT":102193, "CAGTC":387284, "CAGTG":40350, "CAGCA":5930, "CAGCT":7911, "CAGCC":4569, "CAGCG":3033, "CAGGA":26503, "CAGGT":40924, "CAGGC":3413, "CAGGG":21829, "CTAAA":135828, "CTAAT":117657, "CTAAC":21007, "CTAAG":40313, "CTATA":152767, "CTATT":187088, "CTATC":395307, "CTATG":70711, "CTACA":14616, "CTACT":26434, "CTACC":13319, "CTACG":6557, "CTAGA":53597, "CTAGT":165220, "CTAGC":4853, "CTAGG":117710, "CTTAA":134122, "CTTAT":158381, "CTTAC":23531, "CTTAG":54599, "CTTTA":196054, "CTTTT":949609, "CTTTC":404885, "CTTTG":139637, "CTTCA":72421, "CTTCT":459475, "CTTCC":573301, "CTTCG":17169, "CTTGA":80551, "CTTGT":124553, "CTTGC":11148, "CTTGG":46275, "CTCAA":58671, "CTCAT":33367, "CTCAC":27550, "CTCAG":21148, "CTCTA":37140, "CTCTT":415656, "CTCTC":680362, "CTCTG":17570, "CTCCA":392318, "CTCCT":501248, "CTCCC":784241, "CTCCG":5891, "CTCGA":12569, "CTCGT":17768, "CTCGC":4091, "CTCGG":6583, "CTGAA":444815, "CTGAT":62765, "CTGAC":10050, "CTGAG":402032, "CTGTA":76632, "CTGTT":127524, "CTGTC":19741, "CTGTG":45714, "CTGCA":6430, "CTGCT":21268, "CTGCC":5035, "CTGCG":2996, "CTGGA":31282, "CTGGT":116442, "CTGGC":3509, "CTGGG":132566, "CCAAA":68199, "CCAAT":22932, "CCAAC":33725, "CCAAG":12305, "CCATA":14747, "CCATT":31156, "CCATC":19512, "CCATG":8013, "CCACA":12435, "CCACT":30360, "CCACC":23429, "CCACG":5179, "CCAGA":25183, "CCAGT":384342, "CCAGC":4397, "CCAGG":7220, "CCTAA":23296, "CCTAT":21636, "CCTAC":14128, "CCTAG":8323, "CCTTA":25075, "CCTTT":320895, "CCTTC":395571, "CCTTG":18315, "CCTCA":39134, "CCTCT":464000, "CCTCC":667908, "CCTCG":4642, "CCTGA":12122, "CCTGT":16568, "CCTGC":4446, "CCTGG":7054, "CCCAA":49878, "CCCAT":22090, "CCCAC":28192, "CCCAG":27008, "CCCTA":17788, "CCCTT":245929, "CCCTC":473650, "CCCTG":8408, "CCCCA":51415, "CCCCT":290172, "CCCCC":494651, "CCCCG":5664, "CCCGA":6658, "CCCGT":7566, "CCCGC":2524, "CCCGG":2984, "CCGAA":11686, "CCGAT":12858, "CCGAC":3612, "CCGAG":4572, "CCGTA":7193, "CCGTT":25328, "CCGTC":22679, "CCGTG":6269, "CCGCA":1988, "CCGCT":3267, "CCGCC":2394, "CCGCG":1736, "CCGGA":5487, "CCGGT":7186, "CCGGC":2416, "CCGGG":4024, "CGAAA":766436, "CGAAT":765369, "CGAAC":40739, "CGAAG":358009, "CGATA":1027003, "CGATT":1214300, "CGATC":95835, "CGATG":435465, "CGACA":5742, "CGACT":8481, "CGACC":4353, "CGACG":151030, "CGAGA":484982, "CGAGT":565483, "CGAGC":32657, "CGAGG":251152, "CGTAA":907283, "CGTAT":1224503, "CGTAC":83808, "CGTAG":433880, "CGTTA":1322349, "CGTTT":1951666, "CGTTC":137520, "CGTTG":516095, "CGTCA":14700, "CGTCT":485302, "CGTCC":14660, "CGTCG":215960, "CGTGA":402749, "CGTGT":700713, "CGTGC":35048, "CGTGG":234041, "CGCAA":5438, "CGCAT":6707, "CGCAC":2898, "CGCAG":2856, "CGCTA":5508, "CGCTT":11564, "CGCTC":5847, "CGCTG":3066, "CGCCA":1631, "CGCCT":4662, "CGCCC":4640, "CGCCG":1507, "CGCGA":120242, "CGCGT":165647, "CGCGC":17681, "CGCGG":83027, "CGGAA":478881, "CGGAT":644094, "CGGAC":66960, "CGGAG":242974, "CGGTA":562909, "CGGTT":792960, "CGGTC":65945, "CGGTG":298422, "CGGCA":2565, "CGGCT":19296, "CGGCC":2729, "CGGCG":86156, "CGGGA":249085, "CGGGT":374881, "CGGGC":29135, "CGGGG":235438, "GAAAA":20378762, "GAAAT":22745502, "GAAAC":198438, "GAAAG":8017296, "GAATA":16301607, "GAATT":21379820, "GAATC":263731, "GAATG":8179684, "GAACA":45710, "GAACT":440954, "GAACC":24610, "GAACG":304639, "GAAGA":7724479, "GAAGT":8821391, "GAAGC":96106, "GAAGG":3439946, "GATAA":16939283, "GATAT":25391700, "GATAC":232330, "GATAG":9235589, "GATTA":17745793, "GATTT":31325359, "GATTC":443269, "GATTG":10975068, "GATCA":56480, "GATCT":141824, "GATCC":99292, "GATCG":567305, "GATGA":8567837, "GATGT":12750513, "GATGC":96742, "GATGG":4591425, "GACAA":41498, "GACAT":50862, "GACAC":5467, "GACAG":22253, "GACTA":33361, "GACTT":76391, "GACTC":28296, "GACTG":21520, "GACCA":10054, "GACCT":19827, "GACCC":25750, "GACCG":2892, "GACGA":396523, "GACGT":594168, "GACGC":22172, "GACGG":304667, "GAGAA":7568692, "GAGAT":8503450, "GAGAC":94918, "GAGAG":9291071, "GAGTA":7331082, "GAGTT":9854492, "GAGTC":128466, "GAGTG":5505762, "GAGCA":165557, "GAGCT":29446, "GAGCC":13814, "GAGCG":212029, "GAGGA":3997203, "GAGGT":4632489, "GAGGC":51276, "GAGGG":2342566, "GTAAA":19447199, "GTAAT":20548049, "GTAAC":199930, "GTAAG":7514441, "GTATA":26166205, "GTATT":31550060, "GTATC":413548, "GTATG":12866510, "GTACA":58595, "GTACT":444063, "GTACC":23073, "GTACG":561267, "GTAGA":9152051, "GTAGT":10934113, "GTAGC":109047, "GTAGG":4745082, "GTTAA":19948196, "GTTAT":30558104, "GTTAC":311290, "GTTAG":10266109, "GTTTA":28417750, "GTTTT":49760808, "GTTTC":724501, "GTTTG":17649011, "GTTCA":118468, "GTTCT":254842, "GTTCC":136997, "GTTCG":866654, "GTTGA":11755961, "GTTGT":16630478, "GTTGC":139467, "GTTGG":5926730, "GTCAA":88178, "GTCAT":106793, "GTCAC":388694, "GTCAG":39187, "GTCTA":83301, "GTCTT":283473, "GTCTC":123773, "GTCTG":423040, "GTCCA":9097, "GTCCT":113495, "GTCCC":135860, "GTCCG":6725, "GTCGA":547488, "GTCGT":801746, "GTCGC":39172, "GTCGG":421404, "GTGAA":9594759, "GTGAT":12681701, "GTGAC":156556, "GTGAG":4540737, "GTGTA":13985656, "GTGTT":17272098, "GTGTC":282041, "GTGTG":9875139, "GTGCA":31437, "GTGCT":45234, "GTGCC":30393, "GTGCG":227703, "GTGGA":4824816, "GTGGT":6591274, "GTGGC":123211, "GTGGG":3071843, "GCAAA":46933, "GCAAT":46524, "GCAAC":6665, "GCAAG":18522, "GCATA":49724, "GCATT":69865, "GCATC":10241, "GCATG":28781, "GCACA":390937, "GCACT":6598, "GCACC":5171, "GCACG":3177, "GCAGA":21511, "GCAGT":30055, "GCAGC":4469, "GCAGG":13141, "GCTAA":35624, "GCTAT":53713, "GCTAC":5157, "GCTAG":18741, "GCTTA":42432, "GCTTT":147204, "GCTTC":42317, "GCTTG":29600, "GCTCA":5511, "GCTCT":40320, "GCTCC":47769, "GCTCG":4017, "GCTGA":19772, "GCTGT":31543, "GCTGC":3151, "GCTGG":11568, "GCCAA":5369, "GCCAT":6951, "GCCAC":3635, "GCCAG":2912, "GCCTA":4319, "GCCTT":32033, "GCCTC":44905, "GCCTG":3621, "GCCCA":3919, "GCCCT":36164, "GCCCC":52813, "GCCCG":1306, "GCCGA":2497, "GCCGT":25031, "GCCGC":1227, "GCCGG":3795, "GCGAA":319539, "GCGAT":491207, "GCGAC":23869, "GCGAG":256772, "GCGTA":461926, "GCGTT":665156, "GCGTC":87079, "GCGTG":229976, "GCGCA":3695, "GCGCT":4771, "GCGCC":2231, "GCGCG":87430, "GCGGA":216569, "GCGGT":304817, "GCGGC":29815, "GCGGG":175072, "GGAAA":9063491, "GGAAT":8326411, "GGAAC":79407, "GGAAG":4029446, "GGATA":8481352, "GGATT":10688232, "GGATC":157414, "GGATG":4538288, "GGACA":22398, "GGACT":30524, "GGACC":11538, "GGACG":252884, "GGAGA":4139130, "GGAGT":4240282, "GGAGC":45995, "GGAGG":2478672, "GGTAA":8230509, "GGTAT":11785765, "GGTAC":125052, "GGTAG":4522782, "GGTTA":11918995, "GGTTT":16964217, "GGTTC":288723, "GGTTG":6134540, "GGTCA":49979, "GGTCT":79937, "GGTCC":51213, "GGTCG":399587, "GGTGA":4992122, "GGTGT":7091673, "GGTGC":56976, "GGTGG":3566350, "GGCAA":19852, "GGCAT":31328, "GGCAC":2909, "GGCAG":16280, "GGCTA":19924, "GGCTT":59411, "GGCTC":18431, "GGCTG":12513, "GGCCA":4089, "GGCCT":17860, "GGCCC":19224, "GGCCG":8283, "GGCGA":224211, "GGCGT":363662, "GGCGC":22715, "GGCGG":224448, "GGGAA":4082405, "GGGAT":4939744, "GGGAC":67702, "GGGAG":2318877, "GGGTA":4735180, "GGGTT":7694623, "GGGTC":151146, "GGGTG":3140587, "GGGCA":20358, "GGGCT":22847, "GGGCC":15786, "GGGCG":250550, "GGGGA":2708029, "GGGGT":4270281, "GGGGC":149130, "GGGGG":3385375 }, "overrepresented_sequences": { } }, "read2_after_filtering": { "total_reads": 38376265, "total_bases": 5315163692, "q20_bases": 5265592874, "q30_bases": 5114235027, "q40_bases": 5114235027, "total_cycles": 141, "quality_curves": { 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"CTCCC":2345828, "CTCCG":236127, "CTCGA":687481, "CTCGT":357525, "CTCGC":244160, "CTCGG":371139, "CTGAA":49949, "CTGAT":33132, "CTGAC":18074, "CTGAG":15732, "CTGTA":32842, "CTGTT":25474, "CTGTC":16064, "CTGTG":3860, "CTGCA":15585, "CTGCT":10913, "CTGCC":7258, "CTGCG":1628, "CTGGA":23995, "CTGGT":1261, "CTGGC":1143, "CTGGG":54623, "CCAAA":18777536, "CCAAT":11118593, "CCAAC":6223406, "CCAAG":17325, "CCATA":11640900, "CCATT":8604934, "CCATC":4575047, "CCATG":23358, "CCACA":6728660, "CCACT":4244278, "CCACC":3740583, "CCACG":233751, "CCAGA":26322, "CCAGT":13231, "CCAGC":7368, "CCAGG":1778, "CCTAA":10475548, "CCTAT":8769868, "CCTAC":4700534, "CCTAG":12102, "CCTTA":7559808, "CCTTT":7142834, "CCTTC":3392523, "CCTTG":12280, "CCTCA":4166282, "CCTCT":3887591, "CCTCC":2401292, "CCTCG":240517, "CCTGA":19433, "CCTGT":13974, "CCTGC":7192, "CCTGG":1779, "CCCAA":7231022, "CCCAT":4454907, "CCCAC":3233829, "CCCAG":16769, "CCCTA":5272832, "CCCTT":3494494, "CCCTC":2361689, "CCCTG":10922, "CCCCA":3791514, "CCCCT":2887518, "CCCCC":5370817, "CCCCG":221434, "CCCGA":457257, "CCCGT":222036, "CCCGC":166465, "CCCGG":2061, "CCGAA":1031871, "CCGAT":729016, "CCGAC":420191, "CCGAG":2107, "CCGTA":919650, "CCGTT":360770, "CCGTC":296633, "CCGTG":2914, "CCGCA":209449, "CCGCT":207237, "CCGCC":183766, "CCGCG":81499, "CCGGA":2680, "CCGGT":1739, "CCGGC":1127, "CCGGG":931, "CGAAA":2411456, "CGAAT":1353562, "CGAAC":860089, "CGAAG":5861, "CGATA":1453833, "CGATT":907985, "CGATC":684770, "CGATG":3551, "CGACA":645470, "CGACT":471892, "CGACC":372666, "CGACG":213956, "CGAGA":4289, "CGAGT":2503, "CGAGC":1731, "CGAGG":2006, "CGTAA":1268893, "CGTAT":1325831, "CGTAC":551195, "CGTAG":2842, "CGTTA":775340, "CGTTT":676331, "CGTTC":302612, "CGTTG":2986, "CGTCA":522699, "CGTCT":385463, "CGTCC":239973, "CGTCG":518870, "CGTGA":3690, "CGTGT":379188, "CGTGC":1940, "CGTGG":2637, "CGCAA":531918, "CGCAT":358069, "CGCAC":220965, "CGCAG":2303, "CGCTA":451622, "CGCTT":329778, "CGCTC":202064, "CGCTG":1430, "CGCCA":279082, "CGCCT":172891, "CGCCC":159523, "CGCCG":436101, "CGCGA":187850, "CGCGT":105838, "CGCGC":82965, "CGCGG":916, "CGGAA":28945, "CGGAT":2307, "CGGAC":1880, "CGGAG":2140, "CGGTA":2251, "CGGTT":2732, "CGGTC":1551, "CGGTG":369239, "CGGCA":1396, "CGGCT":1185, "CGGCC":1300, "CGGCG":1179, "CGGGA":6755, "CGGGT":721, "CGGGC":706, "CGGGG":2785, "GAAAA":2408381, "GAAAT":1314939, "GAAAC":683696, "GAAAG":712838, "GAATA":1221958, "GAATT":892973, "GAATC":512131, "GAATG":17124, "GAACA":627610, "GAACT":400857, "GAACC":285614, "GAACG":129404, "GAAGA":709666, "GAAGT":55424, "GAAGC":46440, "GAAGG":342021, "GATAA":1239102, "GATAT":955238, "GATAC":494701, "GATAG":20755, "GATTA":770600, "GATTT":727583, "GATTC":330998, "GATTG":2819, "GATCA":458289, "GATCT":777148, "GATCC":193518, "GATCG":109825, "GATGA":20439, "GATGT":4193, "GATGC":2447, "GATGG":17811, "GACAA":963523, "GACAT":376132, "GACAC":218944, "GACAG":3073, "GACTA":408089, "GACTT":285899, "GACTC":145041, "GACTG":1652, "GACCA":259012, "GACCT":159916, "GACCC":135897, "GACCG":64108, "GACGA":116532, "GACGT":78451, "GACGC":46977, "GACGG":1869, "GAGAA":795336, "GAGAT":388948, "GAGAC":162596, "GAGAG":512622, "GAGTA":77341, "GAGTT":53421, "GAGTC":30604, "GAGTG":371040, "GAGCA":60812, "GAGCT":47105, "GAGCC":27599, "GAGCG":138561, "GAGGA":593337, "GAGGT":48794, "GAGGC":39034, "GAGGG":320419, "GTAAA":809435, "GTAAT":478433, "GTAAC":298729, "GTAAG":8695, "GTATA":563419, "GTATT":433321, "GTATC":583094, "GTATG":3363, "GTACA":308652, "GTACT":179709, "GTACC":125517, "GTACG":77976, "GTAGA":346659, "GTAGT":3733, "GTAGC":1735, "GTAGG":374056, "GTTAA":429169, "GTTAT":365425, "GTTAC":195422, "GTTAG":2512, "GTTTA":371661, "GTTTT":336737, "GTTTC":183876, "GTTTG":4260, "GTTCA":189304, "GTTCT":128452, "GTTCC":91457, "GTTCG":33754, "GTTGA":2918, "GTTGT":6070, "GTTGC":10630, "GTTGG":3098, "GTCAA":278340, "GTCAT":260295, "GTCAC":158721, "GTCAG":1987, "GTCTA":224887, "GTCTT":148467, "GTCTC":109014, "GTCTG":2130, "GTCCA":107600, "GTCCT":83070, "GTCCC":71634, "GTCCG":58654, "GTCGA":98733, "GTCGT":439320, "GTCGC":386830, "GTCGG":6869, "GTGAA":10489, "GTGAT":3781, "GTGAC":372288, "GTGAG":7725, "GTGTA":700629, "GTGTT":7495, "GTGTC":39579, "GTGTG":379576, "GTGCA":2017, "GTGCT":10895, "GTGCC":1789, "GTGCG":959, "GTGGA":8737, "GTGGT":378319, "GTGGC":2087, "GTGGG":14256, "GCAAA":387743, "GCAAT":241243, "GCAAC":138621, "GCAAG":2715, "GCATA":243627, "GCATT":174160, "GCATC":100603, "GCATG":1741, "GCACA":130492, "GCACT":93153, "GCACC":56340, "GCACG":34375, "GCAGA":3323, "GCAGT":2726, "GCAGC":1641, "GCAGG":2220, "GCTAA":274725, "GCTAT":232765, "GCTAC":117351, "GCTAG":957, "GCTTA":173955, "GCTTT":168285, "GCTTC":105666, "GCTTG":1236, "GCTCA":113385, "GCTCT":103218, "GCTCC":51110, "GCTCG":32453, "GCTGA":1786, "GCTGT":1414, "GCTGC":1086, "GCTGG":1343, "GCCAA":158555, "GCCAT":148451, "GCCAC":74948, "GCCAG":1485, "GCCTA":110146, "GCCTT":75719, "GCCTC":50209, "GCCTG":1405, "GCCCA":74838, "GCCCT":57015, "GCCCC":61815, "GCCCG":28818, "GCCGA":49737, "GCCGT":384068, "GCCGC":18058, "GCCGG":889, "GCGAA":95074, "GCGAT":68146, "GCGAC":38515, "GCGAG":1823, "GCGTA":57359, "GCGTT":35449, "GCGTC":396175, "GCGTG":2164, "GCGCA":30261, "GCGCT":23138, "GCGCC":22540, "GCGCG":15903, "GCGGA":2153, "GCGGT":813, "GCGGC":868, "GCGGG":2211, "GGAAA":1025745, "GGAAT":357148, "GGAAC":153673, "GGAAG":400619, "GGATA":363495, "GGATT":265910, "GGATC":141542, "GGATG":17384, "GGACA":148307, "GGACT":103125, "GGACC":64144, "GGACG":7805, "GGAGA":565400, "GGAGT":48935, "GGAGC":39263, "GGAGG":342358, "GGTAA":64267, "GGTAT":43400, "GGTAC":26699, "GGTAG":8098, "GGTTA":43916, "GGTTT":37186, "GGTTC":20098, "GGTTG":12938, "GGTCA":31197, "GGTCT":16110, "GGTCC":11957, "GGTCG":366147, "GGTGA":7545, "GGTGT":3972, "GGTGC":1547, "GGTGG":381378, "GGCAA":53672, "GGCAT":33326, "GGCAC":18990, "GGCAG":2256, "GGCTA":44173, "GGCTT":27286, "GGCTC":16410, "GGCTG":1313, "GGCCA":24484, "GGCCT":14070, "GGCCC":12923, "GGCCG":2202, "GGCGA":4065, "GGCGT":2476, "GGCGC":1848, "GGCGG":2330, "GGGAA":785484, "GGGAT":202695, "GGGAC":78633, "GGGAG":325249, "GGGTA":38740, "GGGTT":28433, "GGGTC":15486, "GGGTG":13364, "GGGCA":27276, "GGGCT":22567, "GGGCC":13440, "GGGCG":3464, "GGGGA":342880, "GGGGT":31733, "GGGGC":17484, "GGGGG":1255594 }, "overrepresented_sequences": { } }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/PUM1 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/PUM1 (paired)_R2.fastq.gz -o output/13-wgbs/trimmed/Pum1_R1.fq.gz -O output/13-wgbs/trimmed/Pum1_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs/fastp/Pum1.fastp.json --html output/13-wgbs/fastp/Pum1.fastp.html " }