Bismark report for: output/13-wgbs/trimmed/Qui2_R1.fq.gz and output/13-wgbs/trimmed/Qui2_R2.fq.gz (version: v0.25.1) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-ostrea-chil/data/13-wgbs-genome/ with the specified options: -q --score-min L,0,-0.6 -p 2 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 36627727 Number of paired-end alignments with a unique best hit: 20881563 Mapping efficiency: 57.0% Sequence pairs with no alignments under any condition: 4060145 Sequence pairs did not map uniquely: 11686019 Sequence pairs which were discarded because genomic sequence could not be extracted: 3 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 10451564 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 10429996 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 908365821 Total methylated C's in CpG context: 24045384 Total methylated C's in CHG context: 1137125 Total methylated C's in CHH context: 5126664 Total methylated C's in Unknown context: 122314 Total unmethylated C's in CpG context: 86907051 Total unmethylated C's in CHG context: 153351251 Total unmethylated C's in CHH context: 637798346 Total unmethylated C's in Unknown context: 882366 C methylated in CpG context: 21.7% C methylated in CHG context: 0.7% C methylated in CHH context: 0.8% C methylated in Unknown context (CN or CHN): 12.2% Bismark completed in 0d 0h 52m 17s