Bismark report for: output/13-wgbs/trimmed/Pum3_R1.fq.gz and output/13-wgbs/trimmed/Pum3_R2.fq.gz (version: v0.25.1) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-ostrea-chil/data/13-wgbs-genome/ with the specified options: -q --score-min L,0,-0.6 -p 2 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 32017056 Number of paired-end alignments with a unique best hit: 17918749 Mapping efficiency: 56.0% Sequence pairs with no alignments under any condition: 4101413 Sequence pairs did not map uniquely: 9996894 Sequence pairs which were discarded because genomic sequence could not be extracted: 2 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8969977 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 8948770 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 791587296 Total methylated C's in CpG context: 18757275 Total methylated C's in CHG context: 810304 Total methylated C's in CHH context: 3592465 Total methylated C's in Unknown context: 94984 Total unmethylated C's in CpG context: 77563134 Total unmethylated C's in CHG context: 134340963 Total unmethylated C's in CHH context: 556523155 Total unmethylated C's in Unknown context: 856576 C methylated in CpG context: 19.5% C methylated in CHG context: 0.6% C methylated in CHH context: 0.6% C methylated in Unknown context (CN or CHN): 10.0% Bismark completed in 0d 0h 43m 44s