Variant sites (alt >= 25% at informative depth >= 4 in any oyster): 36 seen in >= 2 oysters: 9 differ between Qui vs Rio (pooled alt-frequency difference >= 0.5): 4 [CpG-destroying: 3] 32453163 G>A Pum=1.0 Qui=0.875 Rio=0.261 32453168 G>A Pum=1.0 Qui=0.875 Rio=0.25 32453285 G>A Pum=1.0 Qui=0.833 Rio=0.333 32453676 T>A Pum=1.0 Qui=0.786 Rio=0.111 10 CpGs in target; 5 testable for SNPs (>= 8 pooled genotype reads at C and G in each of Qui, Rio) sample pop meth% calls CtoT_snp depth Pum1 Pum 79.6 49 0 16.5 Qui1 Qui 71.4 21 0 4.1 Qui2 Qui 29.4 34 0 12.6 Qui3 Qui 34.9 63 0 11.6 Qui4 Qui 31.4 35 0 9.8 Rio1 Rio 87.9 33 0 7.8 Rio2 Rio 48.7 39 0 8.8 Rio3 Rio 64.3 14 0 6.3 Rio4 Rio 86.2 29 0 7.2