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32275858, "TGATC": 388429, "TGATG": 14102845, "TGACA": 64413, "TGACT": 94177, "TGACC": 35896, "TGACG": 571825, "TGAGA": 9012591, "TGAGT": 11411199, "TGAGC": 115349, "TGAGG": 4604423, "TGTAA": 26256973, "TGTAT": 41208928, "TGTAC": 353177, "TGTAG": 13401588, "TGTTA": 32548719, "TGTTT": 52959214, "TGTTC": 654264, "TGTTG": 18893138, "TGTCA": 150337, "TGTCT": 282226, "TGTCC": 144674, "TGTCG": 882426, "TGTGA": 13403418, "TGTGT": 22991932, "TGTGC": 196599, "TGTGG": 7185177, "TGCAA": 72288, "TGCAT": 90499, "TGCAC": 12057, "TGCAG": 37803, "TGCTA": 67458, "TGCTT": 192064, "TGCTC": 51610, "TGCTG": 42886, "TGCCA": 16355, "TGCCT": 46294, "TGCCC": 50729, "TGCCG": 61472, "TGCGA": 421771, "TGCGT": 553195, "TGCGC": 35902, "TGCGG": 236269, "TGGAA": 10592356, "TGGAT": 12214287, "TGGAC": 121069, "TGGAG": 5267354, "TGGTA": 12318974, "TGGTT": 17064134, "TGGTC": 243324, "TGGTG": 8016681, "TGGCA": 40357, "TGGCT": 52918, "TGGCC": 22678, "TGGCG": 431156, "TGGGA": 5305469, "TGGGT": 6805981, "TGGGC": 85301, 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32196, "CAGGT": 52548, "CAGGC": 5133, "CAGGG": 27890, "CTAAA": 164531, "CTAAT": 144961, "CTAAC": 35902, "CTAAG": 50705, "CTATA": 184465, "CTATT": 232303, "CTATC": 1061463, "CTATG": 85540, "CTACA": 25494, "CTACT": 39747, "CTACC": 24530, "CTACG": 9251, "CTAGA": 410550, "CTAGT": 395885, "CTAGC": 8467, "CTAGG": 232037, "CTTAA": 164239, "CTTAT": 196734, "CTTAC": 37555, "CTTAG": 116502, "CTTTA": 250647, "CTTTT": 1268133, "CTTTC": 481826, "CTTTG": 314895, "CTTCA": 158824, "CTTCT": 639015, "CTTCC": 735342, "CTTCG": 25220, "CTTGA": 105438, "CTTGT": 156992, "CTTGC": 16966, "CTTGG": 76859, "CTCAA": 82058, "CTCAT": 51969, "CTCAC": 44073, "CTCAG": 344954, "CTCTA": 118808, "CTCTT": 563045, "CTCTC": 756591, "CTCTG": 28763, "CTCCA": 1858440, "CTCCT": 566515, "CTCCC": 892007, "CTCCG": 11605, "CTCGA": 19315, "CTCGT": 25187, "CTCGC": 6880, "CTCGG": 12429, "CTGAA": 1731697, "CTGAT": 80838, "CTGAC": 19124, "CTGAG": 1229881, "CTGTA": 91163, "CTGTT": 171007, "CTGTC": 29109, "CTGTG": 55414, "CTGCA": 12603, "CTGCT": 43707, "CTGCC": 10370, "CTGCG": 5788, "CTGGA": 50793, "CTGGT": 288974, "CTGGC": 6264, "CTGGG": 286799, "CCAAA": 104261, "CCAAT": 39193, "CCAAC": 53721, "CCAAG": 22616, "CCATA": 26673, "CCATT": 49763, "CCATC": 39362, "CCATG": 11966, "CCACA": 25996, "CCACT": 48212, "CCACC": 40974, "CCACG": 13031, "CCAGA": 1790273, "CCAGT": 1384093, "CCAGC": 10961, "CCAGG": 12920, "CCTAA": 38336, "CCTAT": 36144, "CCTAC": 25370, "CCTAG": 271333, "CCTTA": 73944, "CCTTT": 399903, "CCTTC": 457229, "CCTTG": 28544, "CCTCA": 261906, "CCTCT": 527335, "CCTCC": 758169, "CCTCG": 8901, "CCTGA": 23738, "CCTGT": 24250, "CCTGC": 8317, "CCTGG": 10961, "CCCAA": 87449, "CCCAT": 40067, "CCCAC": 49437, "CCCAG": 1318580, "CCCTA": 208913, "CCCTT": 301057, "CCCTC": 542484, "CCCTG": 16283, "CCCCA": 834004, "CCCCT": 352788, "CCCCC": 621786, "CCCCG": 14649, "CCCGA": 16675, "CCCGT": 16257, "CCCGC": 6253, "CCCGG": 5163, "CCGAA": 20201, "CCGAT": 93616, "CCGAC": 7056, "CCGAG": 10917, "CCGTA": 11973, "CCGTT": 46429, "CCGTC": 65961, "CCGTG": 9361, "CCGCA": 6438, "CCGCT": 7403, "CCGCC": 6090, "CCGCG": 2926, "CCGGA": 11588, "CCGGT": 11625, "CCGGC": 5646, "CCGGG": 6108, "CGAAA": 847382, "CGAAT": 834041, "CGAAC": 51149, "CGAAG": 397565, "CGATA": 1119107, "CGATT": 1325382, "CGATC": 184941, "CGATG": 475352, "CGACA": 8263, "CGACT": 12330, "CGACC": 7428, "CGACG": 167191, "CGAGA": 535784, "CGAGT": 617687, "CGAGC": 37937, "CGAGG": 277036, "CGTAA": 989500, "CGTAT": 1335630, "CGTAC": 95115, "CGTAG": 474574, "CGTTA": 1439552, "CGTTT": 2250355, "CGTTC": 158486, "CGTTG": 567124, "CGTCA": 23358, "CGTCT": 2041760, "CGTCC": 27703, "CGTCG": 239776, "CGTGA": 442773, "CGTGT": 780362, "CGTGC": 39825, "CGTGG": 257293, "CGCAA": 7979, "CGCAT": 9538, "CGCAC": 7114, "CGCAG": 6372, "CGCTA": 7981, "CGCTT": 18066, "CGCTC": 12465, "CGCTG": 6218, "CGCCA": 3944, "CGCCT": 9128, "CGCCC": 8219, "CGCCG": 2631, "CGCGA": 132114, "CGCGT": 182188, "CGCGC": 20780, "CGCGG": 91161, "CGGAA": 3003347, "CGGAT": 704417, "CGGAC": 75309, "CGGAG": 272324, "CGGTA": 614004, "CGGTT": 892809, "CGGTC": 75489, "CGGTG": 326899, "CGGCA": 5727, "CGGCT": 39050, "CGGCC": 4890, "CGGCG": 95299, "CGGGA": 275810, "CGGGT": 419584, "CGGGC": 32897, "CGGGG": 264493, "GAAAA": 22498117, "GAAAT": 24667128, "GAAAC": 226224, "GAAAG": 8879545, "GAATA": 17722457, "GAATT": 23216320, "GAATC": 293686, "GAATG": 8912218, "GAACA": 58183, "GAACT": 1640461, "GAACC": 39458, "GAACG": 334350, "GAAGA": 10781622, "GAAGT": 9631999, "GAAGC": 108917, "GAAGG": 3863439, "GATAA": 18432100, "GATAT": 27593923, "GATAC": 255285, "GATAG": 10079156, "GATTA": 19313344, "GATTT": 34019599, "GATTC": 489355, "GATTG": 11967425, "GATCA": 69042, "GATCT": 230772, "GATCC": 115396, "GATCG": 3300979, "GATGA": 9324701, "GATGT": 13906425, "GATGC": 108463, "GATGG": 5021077, "GACAA": 49564, "GACAT": 60005, "GACAC": 11426, "GACAG": 26995, "GACTA": 39899, "GACTT": 91773, "GACTC": 37460, "GACTG": 26273, "GACCA": 14984, "GACCT": 25939, "GACCC": 32729, "GACCG": 5913, "GACGA": 435043, "GACGT": 652338, "GACGC": 27821, "GACGG": 336357, "GAGAA": 8322681, "GAGAT": 9309811, "GAGAC": 108377, "GAGAG": 10211718, "GAGTA": 8729241, "GAGTT": 10760706, "GAGTC": 148791, "GAGTG": 6070294, "GAGCA": 2203357, "GAGCT": 35618, "GAGCC": 23128, "GAGCG": 233763, "GAGGA": 4401151, "GAGGT": 5088651, "GAGGC": 63658, "GAGGG": 2794482, "GTAAA": 21109279, "GTAAT": 22333658, "GTAAC": 221787, "GTAAG": 8193736, "GTATA": 28489164, "GTATT": 34299867, "GTATC": 453953, "GTATG": 14043490, "GTACA": 69333, "GTACT": 1184204, "GTACC": 28080, "GTACG": 614064, "GTAGA": 9976132, "GTAGT": 11911479, "GTAGC": 119996, "GTAGG": 5194352, "GTTAA": 21671072, "GTTAT": 33189074, "GTTAC": 343192, "GTTAG": 11174317, "GTTTA": 30820007, "GTTTT": 54160486, "GTTTC": 813353, "GTTTG": 19294950, "GTTCA": 137287, "GTTCT": 298335, "GTTCC": 157340, "GTTCG": 949068, "GTTGA": 12783768, "GTTGT": 18097915, "GTTGC": 157076, "GTTGG": 6489354, "GTCAA": 104366, "GTCAT": 128987, "GTCAC": 1309565, "GTCAG": 49292, "GTCTA": 105856, "GTCTT": 472988, "GTCTC": 144061, "GTCTG": 1790892, "GTCCA": 16411, "GTCCT": 138885, "GTCCC": 158402, "GTCCG": 10287, "GTCGA": 601999, "GTCGT": 985201, "GTCGC": 43982, "GTCGG": 472552, "GTGAA": 10463092, "GTGAT": 13840977, "GTGAC": 174850, "GTGAG": 4985782, "GTGTA": 15263418, "GTGTT": 18863421, "GTGTC": 406625, "GTGTG": 11038119, "GTGCA": 37895, "GTGCT": 55156, "GTGCC": 65106, "GTGCG": 254134, "GTGGA": 5265078, "GTGGT": 7220013, "GTGGC": 213877, "GTGGG": 3471331, "GCAAA": 60999, "GCAAT": 55403, "GCAAC": 12082, "GCAAG": 23758, "GCATA": 60060, "GCATT": 84858, "GCATC": 17308, "GCATG": 34395, "GCACA": 2070876, "GCACT": 12918, "GCACC": 14324, "GCACG": 5301, "GCAGA": 31120, "GCAGT": 37663, "GCAGC": 7301, "GCAGG": 17399, "GCTAA": 42272, "GCTAT": 64650, "GCTAC": 7283, "GCTAG": 23590, "GCTTA": 51586, "GCTTT": 216246, "GCTTC": 61072, "GCTTG": 49836, "GCTCA": 9706, "GCTCT": 52404, "GCTCC": 57184, "GCTCG": 9299, "GCTGA": 25962, "GCTGT": 40547, "GCTGC": 4726, "GCTGG": 15254, "GCCAA": 8129, "GCCAT": 13854, "GCCAC": 8888, "GCCAG": 8793, "GCCTA": 8120, "GCCTT": 43353, "GCCTC": 53677, "GCCTG": 6919, "GCCCA": 15285, "GCCCT": 44039, "GCCCC": 64015, "GCCCG": 2682, "GCCGA": 3667, "GCCGT": 71574, "GCCGC": 2298, "GCCGG": 8623, "GCGAA": 350917, "GCGAT": 537297, "GCGAC": 26687, "GCGAG": 282915, "GCGTA": 506179, "GCGTT": 783423, "GCGTC": 149200, "GCGTG": 262371, "GCGCA": 6471, "GCGCT": 6511, "GCGCC": 3700, "GCGCG": 96654, "GCGGA": 238787, "GCGGT": 353080, "GCGGC": 45683, "GCGGG": 201455, "GGAAA": 10050426, "GGAAT": 9087641, "GGAAC": 92094, "GGAAG": 6875026, "GGATA": 9260370, "GGATT": 11695025, "GGATC": 178551, "GGATG": 4974048, "GGACA": 27405, "GGACT": 37568, "GGACC": 15585, "GGACG": 280856, "GGAGA": 4587647, "GGAGT": 4648466, "GGAGC": 55251, "GGAGG": 2842806, "GGTAA": 8993373, "GGTAT": 12877749, "GGTAC": 138530, "GGTAG": 4956322, "GGTTA": 13038738, "GGTTT": 18625151, "GGTTC": 327229, "GGTTG": 6731116, "GGTCA": 63032, "GGTCT": 102164, "GGTCC": 63689, "GGTCG": 465513, "GGTGA": 5467493, "GGTGT": 7888861, "GGTGC": 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433880, "CGTTA": 1322349, "CGTTT": 1951666, "CGTTC": 137520, "CGTTG": 516095, "CGTCA": 14700, "CGTCT": 485302, "CGTCC": 14660, "CGTCG": 215960, "CGTGA": 402749, "CGTGT": 700713, "CGTGC": 35048, "CGTGG": 234041, "CGCAA": 5438, "CGCAT": 6707, "CGCAC": 2898, "CGCAG": 2856, "CGCTA": 5508, "CGCTT": 11564, "CGCTC": 5847, "CGCTG": 3066, "CGCCA": 1631, "CGCCT": 4662, "CGCCC": 4640, "CGCCG": 1507, "CGCGA": 120242, "CGCGT": 165647, "CGCGC": 17681, "CGCGG": 83027, "CGGAA": 478881, "CGGAT": 644094, "CGGAC": 66960, "CGGAG": 242974, "CGGTA": 562909, "CGGTT": 792960, "CGGTC": 65945, "CGGTG": 298422, "CGGCA": 2565, "CGGCT": 19296, "CGGCC": 2729, "CGGCG": 86156, "CGGGA": 249085, "CGGGT": 374881, "CGGGC": 29135, "CGGGG": 235438, "GAAAA": 20378762, "GAAAT": 22745502, "GAAAC": 198438, "GAAAG": 8017296, "GAATA": 16301607, "GAATT": 21379820, "GAATC": 263731, "GAATG": 8179684, "GAACA": 45710, "GAACT": 440954, "GAACC": 24610, "GAACG": 304639, "GAAGA": 7724479, "GAAGT": 8821391, "GAAGC": 96106, "GAAGG": 3439946, "GATAA": 16939283, "GATAT": 25391700, "GATAC": 232330, "GATAG": 9235589, "GATTA": 17745793, "GATTT": 31325359, "GATTC": 443269, "GATTG": 10975068, "GATCA": 56480, "GATCT": 141824, "GATCC": 99292, "GATCG": 567305, "GATGA": 8567837, "GATGT": 12750513, "GATGC": 96742, "GATGG": 4591425, "GACAA": 41498, "GACAT": 50862, "GACAC": 5467, "GACAG": 22253, "GACTA": 33361, "GACTT": 76391, "GACTC": 28296, "GACTG": 21520, "GACCA": 10054, "GACCT": 19827, "GACCC": 25750, "GACCG": 2892, "GACGA": 396523, "GACGT": 594168, "GACGC": 22172, "GACGG": 304667, "GAGAA": 7568692, "GAGAT": 8503450, "GAGAC": 94918, "GAGAG": 9291071, "GAGTA": 7331082, "GAGTT": 9854492, "GAGTC": 128466, "GAGTG": 5505762, "GAGCA": 165557, "GAGCT": 29446, "GAGCC": 13814, "GAGCG": 212029, "GAGGA": 3997203, "GAGGT": 4632489, "GAGGC": 51276, "GAGGG": 2342566, "GTAAA": 19447199, "GTAAT": 20548049, "GTAAC": 199930, "GTAAG": 7514441, "GTATA": 26166205, "GTATT": 31550060, "GTATC": 413548, "GTATG": 12866510, "GTACA": 58595, "GTACT": 444063, "GTACC": 23073, "GTACG": 561267, "GTAGA": 9152051, "GTAGT": 10934113, "GTAGC": 109047, "GTAGG": 4745082, "GTTAA": 19948196, "GTTAT": 30558104, "GTTAC": 311290, "GTTAG": 10266109, "GTTTA": 28417750, "GTTTT": 49760808, "GTTTC": 724501, "GTTTG": 17649011, "GTTCA": 118468, "GTTCT": 254842, "GTTCC": 136997, "GTTCG": 866654, "GTTGA": 11755961, "GTTGT": 16630478, "GTTGC": 139467, "GTTGG": 5926730, "GTCAA": 88178, "GTCAT": 106793, "GTCAC": 388694, "GTCAG": 39187, "GTCTA": 83301, "GTCTT": 283473, "GTCTC": 123773, "GTCTG": 423040, "GTCCA": 9097, "GTCCT": 113495, "GTCCC": 135860, "GTCCG": 6725, "GTCGA": 547488, "GTCGT": 801746, "GTCGC": 39172, "GTCGG": 421404, "GTGAA": 9594759, "GTGAT": 12681701, "GTGAC": 156556, "GTGAG": 4540737, "GTGTA": 13985656, "GTGTT": 17272098, "GTGTC": 282041, "GTGTG": 9875139, "GTGCA": 31437, "GTGCT": 45234, "GTGCC": 30393, "GTGCG": 227703, "GTGGA": 4824816, "GTGGT": 6591274, "GTGGC": 123211, "GTGGG": 3071843, "GCAAA": 46933, "GCAAT": 46524, "GCAAC": 6665, "GCAAG": 18522, "GCATA": 49724, "GCATT": 69865, "GCATC": 10241, "GCATG": 28781, "GCACA": 390937, "GCACT": 6598, "GCACC": 5171, "GCACG": 3177, "GCAGA": 21511, "GCAGT": 30055, "GCAGC": 4469, "GCAGG": 13141, "GCTAA": 35624, "GCTAT": 53713, "GCTAC": 5157, "GCTAG": 18741, "GCTTA": 42432, "GCTTT": 147204, "GCTTC": 42317, "GCTTG": 29600, "GCTCA": 5511, "GCTCT": 40320, "GCTCC": 47769, "GCTCG": 4017, "GCTGA": 19772, "GCTGT": 31543, "GCTGC": 3151, "GCTGG": 11568, "GCCAA": 5369, "GCCAT": 6951, "GCCAC": 3635, "GCCAG": 2912, "GCCTA": 4319, "GCCTT": 32033, "GCCTC": 44905, "GCCTG": 3621, "GCCCA": 3919, "GCCCT": 36164, "GCCCC": 52813, "GCCCG": 1306, "GCCGA": 2497, "GCCGT": 25031, "GCCGC": 1227, "GCCGG": 3795, "GCGAA": 319539, "GCGAT": 491207, "GCGAC": 23869, "GCGAG": 256772, "GCGTA": 461926, "GCGTT": 665156, "GCGTC": 87079, "GCGTG": 229976, "GCGCA": 3695, "GCGCT": 4771, "GCGCC": 2231, "GCGCG": 87430, "GCGGA": 216569, "GCGGT": 304817, "GCGGC": 29815, "GCGGG": 175072, "GGAAA": 9063491, "GGAAT": 8326411, 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"GGGGT": 4270281, "GGGGC": 149130, "GGGGG": 3385375 }, "overrepresented_sequences": {} }, "read2_after_filtering": { "total_reads": 38376265, "total_bases": 5315163692, "q20_bases": 5265592874, "q30_bases": 5114235027, "q40_bases": 5114235027, "total_cycles": 141, "quality_curves": { "A": [ 39.6779, 39.6924, 39.6828, 39.6373, 39.7031, 39.6398, 39.6194, 39.6225, 39.6346, 39.677, 39.553, 39.6297, 39.6335, 39.6169, 39.5721, 39.6345, 39.6343, 39.6765, 39.6244, 39.5844, 39.6376, 39.5827, 39.6339, 39.5984, 39.5774, 39.6018, 39.519, 39.6492, 39.6026, 39.452, 39.5553, 39.5136, 39.5554, 39.5647, 39.6159, 39.4952, 39.5608, 39.4525, 39.6031, 39.5475, 39.5273, 39.5859, 39.5794, 39.5747, 39.5571, 39.5372, 39.5299, 39.5565, 39.5563, 39.5212, 39.563, 39.5018, 39.4776, 39.491, 39.4086, 39.5032, 39.4213, 39.2645, 39.4027, 39.352, 39.1854, 39.3798, 39.3216, 39.4055, 39.235, 39.0966, 39.3671, 39.3542, 39.4246, 39.3827, 39.3573, 39.3824, 39.3263, 39.2283, 39.3106, 39.3752, 39.2968, 39.3922, 39.308, 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19223759, "TTCAC": 9623885, "TTCAG": 19872, "TTCTA": 17108199, "TTCTT": 14156276, "TTCTC": 7389214, "TTCTG": 28611, "TTCCA": 9515458, "TTCCT": 7098602, "TTCCC": 3945380, "TTCCG": 526358, "TTCGA": 924193, "TTCGT": 623002, "TTCGC": 309428, "TTCGG": 2096, "TTGAA": 85390, "TTGAT": 60873, "TTGAC": 30998, "TTGAG": 3273, "TTGTA": 47534, "TTGTT": 47821, "TTGTC": 25420, "TTGTG": 4448, "TTGCA": 24596, "TTGCT": 17992, "TTGCC": 19196, "TTGCG": 2189, "TTGGA": 3333, "TTGGT": 2945, "TTGGC": 1321, "TTGGG": 5298, "TCAAA": 35472998, "TCAAT": 22626133, "TCAAC": 11878885, "TCAAG": 23841, "TCATA": 21004512, "TCATT": 18524940, "TCATC": 8525510, "TCATG": 33137, "TCACA": 12462381, "TCACT": 9261530, "TCACC": 5048664, "TCACG": 386887, "TCAGA": 24759, "TCAGT": 29715, "TCAGC": 9147, "TCAGG": 2347, "TCTAA": 20509291, "TCTAT": 18674333, "TCTAC": 9151308, "TCTAG": 32413, "TCTTA": 13523134, "TCTTT": 13982148, "TCTTC": 7472538, "TCTTG": 20625, "TCTCA": 8130341, "TCTCT": 11664407, "TCTCC": 4033562, "TCTCG": 826545, 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37406, "TGTCT": 27259, "TGTCC": 19472, "TGTCG": 41737, "TGTGA": 375310, "TGTGT": 5774, "TGTGC": 6807, "TGTGG": 7776, "TGCAA": 36609, "TGCAT": 31008, "TGCAC": 17158, "TGCAG": 1898, "TGCTA": 22516, "TGCTT": 19873, "TGCTC": 27424, "TGCTG": 1429, "TGCCA": 15061, "TGCCT": 10526, "TGCCC": 9410, "TGCCG": 9774, "TGCGA": 2542, "TGCGT": 2122, "TGCGC": 2443, "TGCGG": 901, "TGGAA": 19494, "TGGAT": 4737, "TGGAC": 2251, "TGGAG": 30896, "TGGTA": 2318, "TGGTT": 12774, "TGGTC": 367596, "TGGTG": 3371, "TGGCA": 2315, "TGGCT": 1633, "TGGCC": 1564, "TGGCG": 957, "TGGGA": 36932, "TGGGT": 3429, "TGGGC": 1767, "TGGGG": 48777, "CAAAA": 55124188, "CAAAT": 32557816, "CAAAC": 18095251, "CAAAG": 52063, "CAATA": 33930422, "CAATT": 21376302, "CAATC": 11083669, "CAATG": 56327, "CAACA": 17869181, "CAACT": 10455727, "CAACC": 6421350, "CAACG": 509086, "CAAGA": 38282, "CAAGT": 45419, "CAAGC": 13882, "CAAGG": 3300, "CATAA": 28477285, "CATAT": 26184084, "CATAC": 13012899, "CATAG": 28272, "CATTA": 22194712, "CATTT": 21563723, "CATTC": 8219217, "CATTG": 30679, "CATCA": 13068387, "CATCT": 8024023, "CATCC": 4518760, "CATCG": 427092, "CATGA": 50439, "CATGT": 40283, "CATGC": 18223, "CATGG": 1796, "CACAA": 17960918, "CACAT": 12609867, "CACAC": 10071160, "CACAG": 17367, "CACTA": 12589225, "CACTT": 8154582, "CACTC": 5508181, "CACTG": 20740, "CACCA": 7449710, "CACCT": 5028980, "CACCC": 3268083, "CACCG": 313768, "CACGA": 675451, "CACGT": 427546, "CACGC": 218794, "CACGG": 1521, "CAGAA": 36165, "CAGAT": 36907, "CAGAC": 15983, "CAGAG": 3487, "CAGTA": 22009, "CAGTT": 19817, "CAGTC": 13930, "CAGTG": 16441, "CAGCA": 13467, "CAGCT": 9655, "CAGCC": 8579, "CAGCG": 1373, "CAGGA": 3658, "CAGGT": 1604, "CAGGC": 1464, "CAGGG": 2597, "CTAAA": 28512813, "CTAAT": 18362547, "CTAAC": 10383328, "CTAAG": 22645, "CTATA": 24707070, "CTATT": 15763165, "CTATC": 9096536, "CTATG": 36554, "CTACA": 12469398, "CTACT": 7586101, "CTACC": 4515468, "CTACG": 421926, "CTAGA": 27227, "CTAGT": 17342, "CTAGC": 7593, "CTAGG": 12677, "CTTAA": 17692243, "CTTAT": 13440581, "CTTAC": 7486539, "CTTAG": 14218, "CTTTA": 17078626, "CTTTT": 14516995, "CTTTC": 7918628, "CTTTG": 24066, "CTTCA": 9041034, "CTTCT": 6328823, "CTTCC": 3974658, "CTTCG": 348535, "CTTGA": 27872, "CTTGT": 17864, "CTTGC": 8721, "CTTGG": 1805, "CTCAA": 10673683, "CTCAT": 6636155, "CTCAC": 4587816, "CTCAG": 15597, "CTCTA": 9271123, "CTCTT": 5953036, "CTCTC": 8498427, "CTCTG": 20326, "CTCCA": 4718435, "CTCCT": 3347591, "CTCCC": 2345828, "CTCCG": 236127, "CTCGA": 687481, "CTCGT": 357525, "CTCGC": 244160, "CTCGG": 371139, "CTGAA": 49949, "CTGAT": 33132, "CTGAC": 18074, "CTGAG": 15732, "CTGTA": 32842, "CTGTT": 25474, "CTGTC": 16064, "CTGTG": 3860, "CTGCA": 15585, "CTGCT": 10913, "CTGCC": 7258, "CTGCG": 1628, "CTGGA": 23995, "CTGGT": 1261, "CTGGC": 1143, "CTGGG": 54623, "CCAAA": 18777536, "CCAAT": 11118593, "CCAAC": 6223406, "CCAAG": 17325, "CCATA": 11640900, "CCATT": 8604934, "CCATC": 4575047, "CCATG": 23358, "CCACA": 6728660, "CCACT": 4244278, "CCACC": 3740583, 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258231, "AGAAA": 17649038, "AGAAT": 16358268, "AGAAC": 166773, "AGAAG": 7034442, "AGATA": 18716926, "AGATT": 20442751, "AGATC": 4191377, "AGATG": 8708364, "AGACA": 59719, "AGACT": 63609, "AGACC": 31916, "AGACG": 431995, "AGAGA": 11798677, "AGAGT": 8637616, "AGAGC": 3090832, "AGAGG": 4488639, "AGTAA": 15056242, "AGTAT": 21506860, "AGTAC": 217100, "AGTAG": 8200217, "AGTTA": 19271774, "AGTTT": 32124360, "AGTTC": 470760, "AGTTG": 11620352, "AGTCA": 1611522, "AGTCT": 178442, "AGTCC": 131868, "AGTCG": 524572, "AGTGA": 9761383, "AGTGT": 13816566, "AGTGC": 112588, "AGTGG": 4809201, "AGCAA": 58046, "AGCAT": 65359, "AGCAC": 2796326, "AGCAG": 31726, "AGCTA": 41411, "AGCTT": 88003, "AGCTC": 57039, "AGCTG": 25421, "AGCCA": 15730, "AGCCT": 47631, "AGCCC": 59121, "AGCCG": 3948, "AGCGA": 392154, "AGCGT": 457629, "AGCGC": 31886, "AGCGG": 222612, "AGGAA": 7666472, "AGGAT": 7680602, "AGGAC": 94975, "AGGAG": 3883159, "AGGTA": 8642458, "AGGTT": 12526222, "AGGTC": 207226, "AGGTG": 5659528, "AGGCA": 31240, 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23773743, "TAGAC": 272530, "TAGAG": 10057560, "TAGTA": 21328594, "TAGTT": 29166877, "TAGTC": 420562, "TAGTG": 13817564, "TAGCA": 61025, "TAGCT": 87996, "TAGCC": 46358, "TAGCG": 508927, "TAGGA": 8406887, "TAGGT": 12358654, "TAGGC": 131770, "TAGGG": 6437516, "TTAAA": 53018803, "TTAAT": 55518372, "TTAAC": 556158, "TTAAG": 19297339, "TTATA": 65306514, "TTATT": 91162798, "TTATC": 1263043, "TTATG": 31033529, "TTACA": 205173, "TTACT": 287242, "TTACC": 108275, "TTACG": 1411783, "TTAGA": 22372979, "TTAGT": 28427155, "TTAGC": 307158, "TTAGG": 11989329, "TTTAA": 65262550, "TTTAT": 96055973, "TTTAC": 1033132, "TTTAG": 31459264, "TTTTA": 102724105, "TTTTT": 191656566, "TTTTC": 3849075, "TTTTG": 61606626, "TTTCA": 810307, "TTTCT": 1856530, "TTTCC": 1329996, "TTTCG": 2896229, "TTTGA": 39184086, "TTTGT": 57245340, "TTTGC": 542707, "TTTGG": 21234850, "TTCAA": 518905, "TTCAT": 490345, "TTCAC": 123498, "TTCAG": 296638, "TTCTA": 412314, "TTCTT": 1666621, "TTCTC": 1227883, "TTCTG": 315806, "TTCCA": 288702, 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"CTTTA": 292203, "CTTTT": 1446280, "CTTTC": 640277, "CTTTG": 365379, "CTTCA": 199033, "CTTCT": 875508, "CTTCC": 1022873, "CTTCG": 27904, "CTTGA": 117818, "CTTGT": 175819, "CTTGC": 19992, "CTTGG": 94771, "CTCAA": 86377, "CTCAT": 57201, "CTCAC": 48992, "CTCAG": 493469, "CTCTA": 153825, "CTCTT": 747624, "CTCTC": 1092514, "CTCTG": 36449, "CTCCA": 2343642, "CTCCT": 811951, "CTCCC": 1301778, "CTCCG": 15219, "CTCGA": 21037, "CTCGT": 24960, "CTCGC": 6775, "CTCGG": 14943, "CTGAA": 2124869, "CTGAT": 92807, "CTGAC": 24503, "CTGAG": 44042, "CTGTA": 102987, "CTGTT": 199375, "CTGTC": 1342663, "CTGTG": 65796, "CTGCA": 16223, "CTGCT": 61395, "CTGCC": 15676, "CTGCG": 5137, "CTGGA": 56142, "CTGGT": 406011, "CTGGC": 9807, "CTGGG": 254864, "CCAAA": 115557, "CCAAT": 42278, "CCAAC": 59695, "CCAAG": 24359, "CCATA": 28542, "CCATT": 59912, "CCATC": 48349, "CCATG": 15476, "CCACA": 30150, "CCACT": 53140, "CCACC": 50268, "CCACG": 14292, "CCAGA": 2642650, "CCAGT": 1628289, "CCAGC": 16562, "CCAGG": 15386, "CCTAA": 41945, "CCTAT": 39303, "CCTAC": 27329, "CCTAG": 393290, "CCTTA": 94390, "CCTTT": 509171, "CCTTC": 649098, "CCTTG": 33070, "CCTCA": 368322, "CCTCT": 763303, "CCTCC": 1105385, "CCTCG": 9991, "CCTGA": 25673, "CCTGT": 1370529, "CCTGC": 12050, "CCTGG": 15640, "CCCAA": 102217, "CCCAT": 45264, "CCCAC": 55905, "CCCAG": 1954072, "CCCTA": 296525, "CCCTT": 406492, "CCCTC": 786077, "CCCTG": 20308, "CCCCA": 1226986, "CCCCT": 494732, "CCCCC": 873393, "CCCCG": 16442, "CCCGA": 19953, "CCCGT": 17670, "CCCGC": 6628, "CCCGG": 6162, "CCGAA": 23847, "CCGAT": 93874, "CCGAC": 7330, "CCGAG": 12520, "CCGTA": 12505, "CCGTT": 56888, "CCGTC": 102677, "CCGTG": 10303, "CCGCA": 7125, "CCGCT": 8140, "CCGCC": 7599, "CCGCG": 3048, "CCGGA": 14016, "CCGGT": 12905, "CCGGC": 7219, "CCGGG": 7221, "CGAAA": 839287, "CGAAT": 831897, "CGAAC": 53233, "CGAAG": 393914, "CGATA": 1108415, "CGATT": 1355434, "CGATC": 190352, "CGATG": 482711, "CGACA": 8845, "CGACT": 13785, "CGACC": 8987, "CGACG": 171902, "CGAGA": 526053, "CGAGT": 632403, "CGAGC": 40084, "CGAGG": 283800, "CGTAA": 971437, "CGTAT": 1323246, "CGTAC": 95532, "CGTAG": 481735, "CGTTA": 1448049, "CGTTT": 2362517, "CGTTC": 175588, "CGTTG": 597095, "CGTCA": 27872, "CGTCT": 2573909, "CGTCC": 31341, "CGTCG": 259023, "CGTGA": 447143, "CGTGT": 810180, "CGTGC": 43078, "CGTGG": 274428, "CGCAA": 8553, "CGCAT": 9873, "CGCAC": 6739, "CGCAG": 8036, "CGCTA": 8213, "CGCTT": 19184, "CGCTC": 13663, "CGCTG": 8682, "CGCCA": 5100, "CGCCT": 11509, "CGCCC": 9939, "CGCCG": 3569, "CGCGA": 135953, "CGCGT": 187469, "CGCGC": 20977, "CGCGG": 95960, "CGGAA": 3908619, "CGGAT": 720255, "CGGAC": 76613, "CGGAG": 281676, "CGGTA": 617361, "CGGTT": 927432, "CGGTC": 82670, "CGGTG": 336575, "CGGCA": 6628, "CGGCT": 38551, "CGGCC": 6972, "CGGCG": 101123, "CGGGA": 281178, "CGGGT": 435565, "CGGGC": 35419, "CGGGG": 284497, "GAAAA": 21217372, "GAAAT": 23795068, "GAAAC": 227029, "GAAAG": 8446896, "GAATA": 17129615, "GAATT": 23280729, "GAATC": 335440, "GAATG": 8810455, "GAACA": 68077, "GAACT": 1994483, "GAACC": 47121, "GAACG": 333553, "GAAGA": 11305323, "GAAGT": 9536083, "GAAGC": 106123, "GAAGG": 3849425, "GATAA": 17580808, "GATAT": 27154873, "GATAC": 263992, "GATAG": 9792823, "GATTA": 19359075, "GATTT": 34970001, "GATTC": 580691, "GATTG": 12288580, "GATCA": 86011, "GATCT": 296567, "GATCC": 199553, "GATCG": 4378359, "GATGA": 9275942, "GATGT": 14058541, "GATGC": 115414, "GATGG": 5170860, "GACAA": 56632, "GACAT": 71091, "GACAC": 15992, "GACAG": 33791, "GACTA": 45177, "GACTT": 111954, "GACTC": 58008, "GACTG": 31036, "GACCA": 17211, "GACCT": 41535, "GACCC": 54548, "GACCG": 7293, "GACGA": 428174, "GACGT": 659614, "GACGC": 29068, "GACGG": 338020, "GAGAA": 7967884, "GAGAT": 9047177, "GAGAC": 117120, "GAGAG": 8458692, "GAGTA": 7504354, "GAGTT": 11057212, "GAGTC": 167496, "GAGTG": 5765815, "GAGCA": 2906998, "GAGCT": 42497, "GAGCC": 33013, "GAGCG": 238405, "GAGGA": 4296500, "GAGGT": 5248011, "GAGGC": 70155, "GAGGG": 2774423, "GTAAA": 20622704, "GTAAT": 22099994, "GTAAC": 225833, "GTAAG": 8095810, "GTATA": 27860624, "GTATT": 35026542, "GTATC": 510878, "GTATG": 14259850, "GTACA": 81153, "GTACT": 95024, "GTACC": 37967, "GTACG": 620593, "GTAGA": 9835843, "GTAGT": 12218911, "GTAGC": 131081, "GTAGG": 5342271, "GTTAA": 21716534, "GTTAT": 33867096, "GTTAC": 371746, "GTTAG": 11557367, "GTTTA": 31573497, "GTTTT": 56519020, "GTTTC": 934227, "GTTTG": 20296331, "GTTCA": 165872, "GTTCT": 368518, "GTTCC": 235384, "GTTCG": 1018904, "GTTGA": 13213085, "GTTGT": 19213321, "GTTGC": 174197, "GTTGG": 6883045, "GTCAA": 122619, "GTCAT": 161283, "GTCAC": 2753328, "GTCAG": 59203, "GTCTA": 113624, "GTCTT": 592011, "GTCTC": 213863, "GTCTG": 2233669, "GTCCA": 20154, "GTCCT": 198376, "GTCCC": 243149, "GTCCG": 11472, "GTCGA": 626995, "GTCGT": 1074079, "GTCGC": 48406, "GTCGG": 486832, "GTGAA": 10245704, "GTGAT": 14127298, "GTGAC": 183926, "GTGAG": 4972456, "GTGTA": 15295842, "GTGTT": 19711225, "GTGTC": 474405, "GTGTG": 11161832, "GTGCA": 44998, "GTGCT": 65725, "GTGCC": 95844, "GTGCG": 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11496189, "TGGAC": 120097, "TGGAG": 4828087, "TGGTA": 11668755, "TGGTT": 16584975, "TGGTC": 277996, "TGGTG": 7788785, "TGGCA": 38139, "TGGCT": 61637, "TGGCC": 37013, "TGGCG": 337661, "TGGGA": 4919998, "TGGGT": 6579813, "TGGGC": 85426, "TGGGG": 3965680, "CAAAA": 243826, "CAAAT": 197021, "CAAAC": 66734, "CAAAG": 77466, "CAATA": 143920, "CAATT": 215305, "CAATC": 26649, "CAATG": 81187, "CAACA": 25304, "CAACT": 61231, "CAACC": 38679, "CAACG": 12447, "CAAGA": 56037, "CAAGT": 78915, "CAAGC": 8127, "CAAGG": 36882, "CATAA": 136141, "CATAT": 219730, "CATAC": 15645, "CATAG": 72284, "CATTA": 168191, "CATTT": 369652, "CATTC": 46206, "CATTG": 113879, "CATCA": 27699, "CATCT": 45012, "CATCC": 25897, "CATCG": 10414, "CATGA": 74532, "CATGT": 127960, "CATGC": 7878, "CATGG": 46718, "CACAA": 22907, "CACAT": 23205, "CACAC": 410563, "CACAG": 10823, "CACTA": 397893, "CACTT": 72424, "CACTC": 31823, "CACTG": 14078, "CACCA": 30753, "CACCT": 401821, "CACCC": 32741, "CACCG": 4305, "CACGA": 9346, "CACGT": 403153, 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61130, "GACAC": 7423, "GACAG": 28799, "GACTA": 38494, "GACTT": 95199, "GACTC": 47042, "GACTG": 26028, "GACCA": 11877, "GACCT": 34860, "GACCC": 46924, "GACCG": 3504, "GACGA": 390416, "GACGT": 599773, "GACGC": 23094, "GACGG": 306430, "GAGAA": 7258590, "GAGAT": 8277526, "GAGAC": 102450, "GAGAG": 7669116, "GAGTA": 6893382, "GAGTT": 10143476, "GAGTC": 149083, "GAGTG": 5266792, "GAGCA": 171093, "GAGCT": 36651, "GAGCC": 22665, "GAGCG": 217317, "GAGGA": 3906955, "GAGGT": 4786913, "GAGGC": 58388, "GAGGG": 2341923, "GTAAA": 18998947, "GTAAT": 20340538, "GTAAC": 203958, "GTAAG": 7424819, "GTATA": 25616280, "GTATT": 32211716, "GTATC": 470250, "GTATG": 13027666, "GTACA": 69168, "GTACT": 83861, "GTACC": 33806, "GTACG": 566313, "GTAGA": 9025006, "GTAGT": 11219394, "GTAGC": 119613, "GTAGG": 4875669, "GTTAA": 19977051, "GTTAT": 31175078, "GTTAC": 336647, "GTTAG": 10613351, "GTTTA": 29097398, "GTTTT": 51921658, "GTTTC": 841704, "GTTTG": 18568951, "GTTCA": 144268, "GTTCT": 325276, "GTTCC": 213478, 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7410, "TTGTA": 70407, "TTGTT": 64461, "TTGTC": 32178, "TTGTG": 8656, "TTGCA": 37739, "TTGCT": 27237, "TTGCC": 23595, "TTGCG": 3033, "TTGGA": 7723, "TTGGT": 6220, "TTGGC": 3949, "TTGGG": 8879, "TCAAA": 36403785, "TCAAT": 22618563, "TCAAC": 12324662, "TCAAG": 35705, "TCATA": 21014033, "TCATT": 17876727, "TCATC": 8532989, "TCATG": 48043, "TCACA": 12732392, "TCACT": 9009935, "TCACC": 5247625, "TCACG": 394232, "TCAGA": 38229, "TCAGT": 130988, "TCAGC": 14360, "TCAGG": 6261, "TCTAA": 20587603, "TCTAT": 17592170, "TCTAC": 9081428, "TCTAG": 39874, "TCTTA": 13154169, "TCTTT": 13047745, "TCTTC": 7347085, "TCTTG": 30506, "TCTCA": 8059544, "TCTCT": 10266048, "TCTCC": 3939555, "TCTCG": 834059, "TCTGA": 71164, "TCTGT": 45822, "TCTGC": 20070, "TCTGG": 80898, "TCCAA": 12256753, "TCCAT": 8812559, "TCCAC": 4993346, "TCCAG": 21569, "TCCTA": 7544971, "TCCTT": 5816821, "TCCTC": 3770387, "TCCTG": 21382, "TCCCA": 4882775, "TCCCT": 3396045, "TCCCC": 2715809, "TCCCG": 246733, "TCCGA": 792393, "TCCGT": 491979, 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output/13-wgbs-hapa/trimmed/Qui1_R1.fq.gz -O output/13-wgbs-hapa/trimmed/Qui1_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs-hapa/fastp/Qui1.fastp.json --html output/13-wgbs-hapa/fastp/Qui1.fastp.html " }, "Qui2 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 74497804, "total_bases": 11249168404, "q20_bases": 11099839912, "q30_bases": 10711151271, "q20_rate": 0.986725, "q30_rate": 0.952173, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.213081 }, "after_filtering": { "total_reads": 73255454, "total_bases": 10133522387, "q20_bases": 10035578092, "q30_bases": 9728707541, "q20_rate": 0.990335, "q30_rate": 0.960052, "read1_mean_length": 138, "read2_mean_length": 138, "gc_content": 0.187922 } }, "filtering_result": { "passed_filter_reads": 73255454, "low_quality_reads": 644014, 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3396808, "CCCAG": 88233, "CCCTA": 5202275, "CCCTT": 3628471, "CCCTC": 2632031, "CCCTG": 24585, "CCCCA": 4056306, "CCCCT": 3110390, "CCCCC": 6650651, "CCCCG": 259988, "CCCGA": 504737, "CCCGT": 247263, "CCCGC": 198576, "CCCGG": 15681, "CCGAA": 1135380, "CCGAT": 829147, "CCGAC": 470838, "CCGAG": 13123, "CCGTA": 1291917, "CCGTT": 412797, "CCGTC": 346031, "CCGTG": 19899, "CCGCA": 238651, "CCGCT": 244872, "CCGCC": 217964, "CCGCG": 95155, "CCGGA": 11749, "CCGGT": 8778, "CCGGC": 11519, "CCGGG": 8065, "CGAAA": 2626105, "CGAAT": 1478870, "CGAAC": 919320, "CGAAG": 34948, "CGATA": 1545539, "CGATT": 1035447, "CGATC": 805337, "CGATG": 8581, "CGACA": 701053, "CGACT": 521343, "CGACC": 419965, "CGACG": 238006, "CGAGA": 23892, "CGAGT": 10687, "CGAGC": 8362, "CGAGG": 13534, "CGTAA": 1360673, "CGTAT": 1748093, "CGTAC": 603479, "CGTAG": 18091, "CGTTA": 854074, "CGTTT": 786436, "CGTTC": 344886, "CGTTG": 13119, "CGTCA": 569167, "CGTCT": 447239, "CGTCC": 272941, "CGTCG": 2115480, "CGTGA": 11337, "CGTGT": 1832880, "CGTGC": 8750, "CGTGG": 51669, "CGCAA": 581962, "CGCAT": 395624, "CGCAC": 244590, "CGCAG": 12345, "CGCTA": 491479, "CGCTT": 375721, "CGCTC": 230741, "CGCTG": 5822, "CGCCA": 331551, "CGCCT": 199246, "CGCCC": 189377, "CGCCG": 786178, "CGCGA": 212294, "CGCGT": 126529, "CGCGC": 95799, "CGCGG": 5028, "CGGAA": 2687239, "CGGAT": 15076, "CGGAC": 8228, "CGGAG": 24100, "CGGTA": 7783, "CGGTT": 12872, "CGGTC": 5496, "CGGTG": 810976, "CGGCA": 7630, "CGGCT": 1019314, "CGGCC": 12351, "CGGCG": 7830, "CGGGA": 39140, "CGGGT": 7695, "CGGGC": 5010, "CGGGG": 24029, "GAAAA": 7734332, "GAAAT": 3338387, "GAAAC": 1774501, "GAAAG": 5074019, "GAATA": 3001120, "GAATT": 2169717, "GAATC": 1461710, "GAATG": 68230, "GAACA": 1648053, "GAACT": 1012232, "GAACC": 733345, "GAACG": 198942, "GAAGA": 9580860, "GAAGT": 344578, "GAAGC": 298689, "GAAGG": 5051943, "GATAA": 2828918, "GATAT": 1997191, "GATAC": 1165418, "GATAG": 78295, "GATTA": 1937684, "GATTT": 1556270, "GATTC": 878490, "GATTG": 16952, "GATCA": 1529908, 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"GGGTC": 22041, "GGGTG": 18574, "GGGCA": 28357, "GGGCT": 24728, "GGGCC": 19683, "GGGCG": 5368, "GGGGA": 372691, "GGGGT": 38191, "GGGGC": 21196, "GGGGG": 1181578 }, "overrepresented_sequences": {} }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/Qui2 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/Qui2 (paired)_R2.fastq.gz -o output/13-wgbs-hapa/trimmed/Qui2_R1.fq.gz -O output/13-wgbs-hapa/trimmed/Qui2_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs-hapa/fastp/Qui2.fastp.json --html output/13-wgbs-hapa/fastp/Qui2.fastp.html " }, "Qui3 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 84275732, "total_bases": 12725635532, "q20_bases": 12567504402, "q30_bases": 12168953674, "q20_rate": 0.987574, "q30_rate": 0.956255, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.212825 }, "after_filtering": { "total_reads": 82892660, "total_bases": 11447336864, "q20_bases": 11346828076, "q30_bases": 11038498745, "q20_rate": 0.99122, "q30_rate": 0.964285, "read1_mean_length": 138, "read2_mean_length": 138, "gc_content": 0.187414 } }, "filtering_result": { "passed_filter_reads": 82892660, "low_quality_reads": 706846, "too_many_N_reads": 1614, "too_short_reads": 674612, "too_long_reads": 0 }, "duplication": { "rate": 0.0601871 }, "insert_size": { "peak": 183, "unknown": 930966, "histogram": [ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3239, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 393, 395, 347, 441, 374, 366, 400, 342, 378, 388, 350, 362, 356, 389, 373, 390, 387, 408, 411, 444, 415, 450, 455, 449, 544, 531, 559, 498, 587, 521, 624, 588, 633, 633, 666, 710, 686, 726, 771, 804, 805, 834, 881, 917, 995, 1032, 1040, 1052, 1106, 1191, 1228, 1290, 1335, 1374, 1434, 1593, 1600, 1671, 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11687, "TTGTA": 71686, "TTGTT": 80441, "TTGTC": 46649, "TTGTG": 24613, "TTGCA": 36998, "TTGCT": 27995, "TTGCC": 44700, "TTGCG": 5137, "TTGGA": 16336, "TTGGT": 14073, "TTGGC": 4890, "TTGGG": 25137, "TCAAA": 39338527, "TCAAT": 26126709, "TCAAC": 13303608, "TCAAG": 102996, "TCATA": 23224286, "TCATT": 20921467, "TCATC": 9493174, "TCATG": 1114212, "TCACA": 13571051, "TCACT": 10054902, "TCACC": 5696329, "TCACG": 482021, "TCAGA": 252099, "TCAGT": 44324, "TCAGC": 17415, "TCAGG": 10792, "TCTAA": 22726831, "TCTAT": 20450757, "TCTAC": 10028579, "TCTAG": 158912, "TCTTA": 15057024, "TCTTT": 15661033, "TCTTC": 8519412, "TCTTG": 34236, "TCTCA": 8976304, "TCTCT": 12900444, "TCTCC": 4639833, "TCTCG": 1516358, "TCTGA": 77414, "TCTGT": 59830, "TCTGC": 21795, "TCTGG": 101894, "TCCAA": 13318330, "TCCAT": 9868702, "TCCAC": 5475352, "TCCAG": 70491, "TCCTA": 8420189, "TCCTT": 6803144, "TCCTC": 4375646, "TCCTG": 71877, "TCCCA": 5364446, "TCCCT": 3939613, "TCCCC": 3318015, "TCCCG": 298546, "TCCGA": 966988, 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18099, "TGCCC": 20678, "TGCCG": 27704, "TGCGA": 5567, "TGCGT": 5243, "TGCGC": 5379, "TGCGG": 4137, "TGGAA": 72226, "TGGAT": 13651, "TGGAC": 7316, "TGGAG": 117175, "TGGTA": 8673, "TGGTT": 56744, "TGGTC": 897163, "TGGTG": 1132374, "TGGCA": 7472, "TGGCT": 5444, "TGGCC": 5639, "TGGCG": 6317, "TGGGA": 110260, "TGGGT": 18611, "TGGGC": 7272, "TGGGG": 186813, "CAAAA": 60931391, "CAAAT": 35817591, "CAAAC": 19924598, "CAAAG": 208262, "CAATA": 36616649, "CAATT": 23736616, "CAATC": 13302553, "CAATG": 77190, "CAACA": 19636956, "CAACT": 11560935, "CAACC": 7382765, "CAACG": 599882, "CAAGA": 262295, "CAAGT": 41290, "CAAGC": 27042, "CAAGG": 16140, "CATAA": 31110991, "CATAT": 28726793, "CATAC": 13989274, "CATAG": 187735, "CATTA": 24484582, "CATTT": 24177852, "CATTC": 9118278, "CATTG": 52171, "CATCA": 14282677, "CATCT": 8918987, "CATCC": 5019559, "CATCG": 507713, "CATGA": 72041, "CATGT": 64493, "CATGC": 27458, "CATGG": 1119358, "CACAA": 19633192, "CACAT": 13653376, "CACAC": 10488329, "CACAG": 81538, 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4725104, "CCTCT": 4443227, "CCTCC": 2976369, "CCTCG": 300005, "CCTGA": 32757, "CCTGT": 29603, "CCTGC": 14451, "CCTGG": 67314, "CCCAA": 8324024, "CCCAT": 5009097, "CCCAC": 3917822, "CCCAG": 91986, "CCCTA": 5969382, "CCCTT": 4067249, "CCCTC": 2938256, "CCCTG": 40735, "CCCCA": 4665943, "CCCCT": 3538936, "CCCCC": 8110823, "CCCCG": 298578, "CCCGA": 562239, "CCCGT": 267815, "CCCGC": 211160, "CCCGG": 27406, "CCGAA": 1256413, "CCGAT": 898275, "CCGAC": 501948, "CCGAG": 11124, "CCGTA": 1443784, "CCGTT": 435225, "CCGTC": 374774, "CCGTG": 14766, "CCGCA": 256648, "CCGCT": 250215, "CCGCC": 236774, "CCGCG": 101207, "CCGGA": 11304, "CCGGT": 21425, "CCGGC": 4952, "CCGGG": 8244, "CGAAA": 2904768, "CGAAT": 1618268, "CGAAC": 1014087, "CGAAG": 37948, "CGATA": 1691481, "CGATT": 1087168, "CGATC": 898104, "CGATG": 7383, "CGACA": 766229, "CGACT": 557160, "CGACC": 456998, "CGACG": 261326, "CGAGA": 24159, "CGAGT": 8165, "CGAGC": 7764, "CGAGG": 13060, "CGTAA": 1480651, "CGTAT": 1927885, "CGTAC": 651500, "CGTAG": 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8255, "TTCAA": 28215856, "TTCAT": 20908316, "TTCAC": 10289627, "TTCAG": 26984, "TTCTA": 18707712, "TTCTT": 15585392, "TTCTC": 8100293, "TTCTG": 39067, "TTCCA": 10358351, "TTCCT": 7779816, "TTCCC": 4318582, "TTCCG": 616879, "TTCGA": 1092449, "TTCGT": 724014, "TTCGC": 360288, "TTCGG": 3021, "TTGAA": 114984, "TTGAT": 82573, "TTGAC": 40820, "TTGAG": 5346, "TTGTA": 64167, "TTGTT": 64195, "TTGTC": 32231, "TTGTG": 8729, "TTGCA": 32720, "TTGCT": 24250, "TTGCC": 22295, "TTGCG": 3172, "TTGGA": 5646, "TTGGT": 5553, "TTGGC": 2707, "TTGGG": 7406, "TCAAA": 38180017, "TCAAT": 24666085, "TCAAC": 12809327, "TCAAG": 31208, "TCATA": 22628486, "TCATT": 20064168, "TCATC": 9218048, "TCATG": 405757, "TCACA": 13159480, "TCACT": 9706222, "TCACC": 5441672, "TCACG": 444638, "TCAGA": 33752, "TCAGT": 27451, "TCAGC": 12131, "TCAGG": 4501, "TCTAA": 22206037, "TCTAT": 19991803, "TCTAC": 9774901, "TCTAG": 40901, "TCTTA": 14704492, "TCTTT": 15280466, "TCTTC": 8176916, "TCTTG": 27607, "TCTCA": 8677827, "TCTCT": 12435068, "TCTCC": 4379293, "TCTCG": 941054, "TCTGA": 67257, "TCTGT": 42396, "TCTGC": 17117, "TCTGG": 93318, "TCCAA": 12889646, "TCCAT": 9566946, "TCCAC": 5225495, "TCCAG": 19746, "TCCTA": 8157531, "TCCTT": 6532725, "TCCTC": 4134532, "TCCTG": 36618, "TCCCA": 5104552, "TCCCT": 3712322, "TCCCC": 2881667, "TCCCG": 274545, "TCCGA": 897844, "TCCGT": 564383, "TCCGC": 245976, "TCCGG": 2234, "TCGAA": 1792891, "TCGAT": 1179722, "TCGAC": 620525, "TCGAG": 3393, "TCGTA": 1036177, "TCGTT": 683051, "TCGTC": 442453, "TCGTG": 421913, "TCGCA": 431368, "TCGCT": 400802, "TCGCC": 659040, "TCGCG": 137524, "TCGGA": 33791, "TCGGT": 411501, "TCGGC": 2327, "TCGGG": 8443, "TGAAA": 204888, "TGAAT": 112691, "TGAAC": 58185, "TGAAG": 17873, "TGATA": 125757, "TGATT": 86297, "TGATC": 43764, "TGATG": 5118, "TGACA": 67492, "TGACT": 37344, "TGACC": 33200, "TGACG": 5028, "TGAGA": 24881, "TGAGT": 6518, "TGAGC": 3481, "TGAGG": 28351, "TGTAA": 98314, "TGTAT": 94032, "TGTAC": 38882, "TGTAG": 782942, "TGTTA": 65452, "TGTTT": 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"GAGGT": 59634, "GAGGC": 46711, "GAGGG": 382965, "GTAAA": 923231, "GTAAT": 548032, "GTAAC": 341684, "GTAAG": 11927, "GTATA": 648511, "GTATT": 495635, "GTATC": 649077, "GTATG": 4747, "GTACA": 347459, "GTACT": 210366, "GTACC": 141171, "GTACG": 91877, "GTAGA": 383878, "GTAGT": 5334, "GTAGC": 2629, "GTAGG": 418080, "GTTAA": 499822, "GTTAT": 431994, "GTTAC": 215814, "GTTAG": 3756, "GTTTA": 422431, "GTTTT": 399336, "GTTTC": 217006, "GTTTG": 5976, "GTTCA": 227457, "GTTCT": 154236, "GTTCC": 107675, "GTTCG": 39108, "GTTGA": 4590, "GTTGT": 9071, "GTTGC": 11510, "GTTGG": 4478, "GTCAA": 721792, "GTCAT": 303623, "GTCAC": 186079, "GTCAG": 3531, "GTCTA": 265829, "GTCTT": 173514, "GTCTC": 130221, "GTCTG": 3639, "GTCCA": 152590, "GTCCT": 102095, "GTCCC": 83279, "GTCCG": 72431, "GTCGA": 116751, "GTCGT": 496358, "GTCGC": 431952, "GTCGG": 9188, "GTGAA": 12043, "GTGAT": 5924, "GTGAC": 3636, "GTGAG": 10876, "GTGTA": 790129, "GTGTT": 9241, "GTGTC": 465948, "GTGTG": 12630, "GTGCA": 3689, "GTGCT": 15363, "GTGCC": 2845, "GTGCG": 1522, "GTGGA": 11868, "GTGGT": 421621, "GTGGC": 3138, "GTGGG": 17958, "GCAAA": 429362, "GCAAT": 275296, "GCAAC": 155394, "GCAAG": 4409, "GCATA": 267670, "GCATT": 193371, "GCATC": 112464, "GCATG": 3518, "GCACA": 144660, "GCACT": 105876, "GCACC": 63365, "GCACG": 37845, "GCAGA": 5590, "GCAGT": 4531, "GCAGC": 3208, "GCAGG": 4237, "GCTAA": 309043, "GCTAT": 271174, "GCTAC": 132060, "GCTAG": 1855, "GCTTA": 191484, "GCTTT": 192768, "GCTTC": 118630, "GCTTG": 2672, "GCTCA": 134960, "GCTCT": 127206, "GCTCC": 59850, "GCTCG": 38679, "GCTGA": 3748, "GCTGT": 3022, "GCTGC": 2726, "GCTGG": 3472, "GCCAA": 192488, "GCCAT": 182627, "GCCAC": 93272, "GCCAG": 3143, "GCCTA": 129386, "GCCTT": 92391, "GCCTC": 60430, "GCCTG": 3409, "GCCCA": 94160, "GCCCT": 71929, "GCCCC": 74953, "GCCCG": 35136, "GCCGA": 58210, "GCCGT": 426220, "GCCGC": 21705, "GCCGG": 1618, "GCGAA": 111737, "GCGAT": 78481, "GCGAC": 45006, "GCGAG": 2518, "GCGTA": 66119, "GCGTT": 41283, "GCGTC": 443265, "GCGTG": 2806, "GCGCA": 34286, "GCGCT": 28085, "GCGCC": 27003, "GCGCG": 19861, "GCGGA": 3134, "GCGGT": 1442, "GCGGC": 1733, "GCGGG": 3162, "GGAAA": 1144165, "GGAAT": 388593, "GGAAC": 180040, "GGAAG": 465166, "GGATA": 392605, "GGATT": 296705, "GGATC": 189931, "GGATG": 23518, "GGACA": 168527, "GGACT": 115544, "GGACC": 92521, "GGACG": 9615, "GGAGA": 663695, "GGAGT": 59289, "GGAGC": 46191, "GGAGG": 409842, "GGTAA": 73440, "GGTAT": 43535, "GGTAC": 30755, "GGTAG": 10874, "GGTTA": 54216, "GGTTT": 41646, "GGTTC": 26523, "GGTTG": 14411, "GGTCA": 47109, "GGTCT": 21089, "GGTCC": 18739, "GGTCG": 408776, "GGTGA": 10654, "GGTGT": 418393, "GGTGC": 2877, "GGTGG": 424744, "GGCAA": 55190, "GGCAT": 32415, "GGCAC": 20986, "GGCAG": 4392, "GGCTA": 49628, "GGCTT": 29066, "GGCTC": 21641, "GGCTG": 3405, "GGCCA": 36870, "GGCCT": 19700, "GGCCC": 20426, "GGCCG": 3926, "GGCGA": 4905, "GGCGT": 3242, "GGCGC": 2659, "GGCGG": 3553, "GGGAA": 895975, "GGGAT": 237645, "GGGAC": 95849, "GGGAG": 388463, "GGGTA": 44055, "GGGTT": 34804, "GGGTC": 23516, "GGGTG": 18946, "GGGCA": 29109, "GGGCT": 26599, "GGGCC": 20717, "GGGCG": 4502, "GGGGA": 407042, "GGGGT": 39818, "GGGGC": 21751, "GGGGG": 1414489 }, "overrepresented_sequences": {} }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/Qui3 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/Qui3 (paired)_R2.fastq.gz -o output/13-wgbs-hapa/trimmed/Qui3_R1.fq.gz -O output/13-wgbs-hapa/trimmed/Qui3_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs-hapa/fastp/Qui3.fastp.json --html output/13-wgbs-hapa/fastp/Qui3.fastp.html " }, "Qui4 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 70096496, "total_bases": 10584570896, "q20_bases": 10450858325, "q30_bases": 10097793159, "q20_rate": 0.987367, "q30_rate": 0.954011, "read1_mean_length": 151, "read2_mean_length": 151, 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36257, "GAGCC": 27089, "GAGCG": 214872, "GAGGA": 3935776, "GAGGT": 4615958, "GAGGC": 56220, "GAGGG": 2717372, "GTAAA": 18659718, "GTAAT": 19769160, "GTAAC": 199403, "GTAAG": 7332786, "GTATA": 25261854, "GTATT": 30856044, "GTATC": 454608, "GTATG": 12730246, "GTACA": 1271710, "GTACT": 81543, "GTACC": 35033, "GTACG": 556387, "GTAGA": 8893288, "GTAGT": 10934360, "GTAGC": 119117, "GTAGG": 4710236, "GTTAA": 19417286, "GTTAT": 29831273, "GTTAC": 328544, "GTTAG": 10183863, "GTTTA": 27797724, "GTTTT": 49385988, "GTTTC": 805009, "GTTTG": 17846909, "GTTCA": 133614, "GTTCT": 314970, "GTTCC": 203426, "GTTCG": 891072, "GTTGA": 11712518, "GTTGT": 16851090, "GTTGC": 153422, "GTTGG": 6092261, "GTCAA": 99112, "GTCAT": 120640, "GTCAC": 1409858, "GTCAG": 47257, "GTCTA": 100193, "GTCTT": 245583, "GTCTC": 183848, "GTCTG": 2035469, "GTCCA": 16700, "GTCCT": 162445, "GTCCC": 219388, "GTCCG": 9755, "GTCGA": 564483, "GTCGT": 758755, "GTCGC": 42538, "GTCGG": 431084, "GTGAA": 9340892, "GTGAT": 12733251, "GTGAC": 162847, "GTGAG": 4583986, "GTGTA": 13951826, "GTGTT": 17554770, "GTGTC": 269224, "GTGTG": 10289879, "GTGCA": 35750, "GTGCT": 55649, "GTGCC": 22011, "GTGCG": 232615, "GTGGA": 4803547, "GTGGT": 6923622, "GTGGC": 90681, "GTGGG": 3254865, "GCAAA": 57112, "GCAAT": 54306, "GCAAC": 11485, "GCAAG": 22963, "GCATA": 57901, "GCATT": 79282, "GCATC": 8482, "GCATG": 34141, "GCACA": 2435840, "GCACT": 10789, "GCACC": 13141, "GCACG": 4627, "GCAGA": 31519, "GCAGT": 34489, "GCAGC": 4755, "GCAGG": 17784, "GCTAA": 40157, "GCTAT": 61419, "GCTAC": 6418, "GCTAG": 21786, "GCTTA": 49163, "GCTTT": 152843, "GCTTC": 52264, "GCTTG": 37170, "GCTCA": 8621, "GCTCT": 59681, "GCTCC": 74456, "GCTCG": 9533, "GCTGA": 26946, "GCTGT": 39368, "GCTGC": 4999, "GCTGG": 16560, "GCCAA": 7775, "GCCAT": 6080, "GCCAC": 8551, "GCCAG": 7161, "GCCTA": 7773, "GCCTT": 44263, "GCCTC": 66601, "GCCTG": 6882, "GCCCA": 15234, "GCCCT": 53948, "GCCCC": 86244, "GCCCG": 2650, "GCCGA": 3756, "GCCGT": 5004, "GCCGC": 2181, "GCCGG": 3311, "GCGAA": 316902, "GCGAT": 491586, "GCGAC": 25266, "GCGAG": 257708, "GCGTA": 454465, "GCGTT": 630604, "GCGTC": 54948, "GCGTG": 244263, "GCGCA": 5382, "GCGCT": 5786, "GCGCC": 3365, "GCGCG": 88181, "GCGGA": 219962, "GCGGT": 310481, "GCGGC": 23449, "GCGGG": 198326, "GGAAA": 8833527, "GGAAT": 8118765, "GGAAC": 83539, "GGAAG": 6955423, "GGATA": 8160884, "GGATT": 10630785, "GGATC": 186046, "GGATG": 4536223, "GGACA": 25504, "GGACT": 39969, "GGACC": 19991, "GGACG": 251983, "GGAGA": 4064482, "GGAGT": 4210584, "GGAGC": 52138, "GGAGG": 2769335, "GGTAA": 8024377, "GGTAT": 11630631, "GGTAC": 1361936, "GGTAG": 4539023, "GGTTA": 11841167, "GGTTT": 17458123, "GGTTC": 333643, "GGTTG": 6320171, "GGTCA": 56815, "GGTCT": 115970, "GGTCC": 91500, "GGTCG": 397522, "GGTGA": 4997431, "GGTGT": 7399976, "GGTGC": 62692, "GGTGG": 3847448, "GGCAA": 24758, "GGCAT": 29576, "GGCAC": 7073, "GGCAG": 19992, "GGCTA": 23330, "GGCTT": 68945, "GGCTC": 33678, "GGCTG": 17690, "GGCCA": 5939, "GGCCT": 30539, "GGCCC": 38236, "GGCCG": 3759, 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56781, "CACTC": 23806, "CACTG": 9440, "CACCA": 22634, "CACCT": 13489, "CACCC": 24610, "CACCG": 3452, "CACGA": 303434, "CACGT": 311299, "CACGC": 3359, "CACGG": 300692, "CAGAA": 57091, "CAGAT": 74528, "CAGAC": 8180, "CAGAG": 29709, "CAGTA": 56466, "CAGTT": 98780, "CAGTC": 305763, "CAGTG": 40525, "CAGCA": 6233, "CAGCT": 6056, "CAGCC": 4861, "CAGCG": 3239, "CAGGA": 26393, "CAGGT": 39680, "CAGGC": 3724, "CAGGG": 22201, "CTAAA": 124497, "CTAAT": 107785, "CTAAC": 18111, "CTAAG": 37226, "CTATA": 139720, "CTATT": 178675, "CTATC": 23054, "CTATG": 68231, "CTACA": 12962, "CTACT": 22848, "CTACC": 11248, "CTACG": 5929, "CTAGA": 50458, "CTAGT": 60607, "CTAGC": 4394, "CTAGG": 34175, "CTTAA": 124112, "CTTAT": 147723, "CTTAC": 20574, "CTTAG": 53338, "CTTTA": 180108, "CTTTT": 808619, "CTTTC": 437384, "CTTTG": 129089, "CTTCA": 62542, "CTTCT": 427803, "CTTCC": 695948, "CTTCG": 16961, "CTTGA": 75560, "CTTGT": 117989, "CTTGC": 10720, "CTTGG": 45053, "CTCAA": 49907, "CTCAT": 28812, "CTCAC": 23800, "CTCAG": 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45133093, "GTTTC": 725401, "GTTTG": 16215031, "GTTCA": 115444, "GTTCT": 276431, "GTTCC": 184507, "GTTCG": 812151, "GTTGA": 10747366, "GTTGT": 15440499, "GTTGC": 137312, "GTTGG": 5514399, "GTCAA": 85087, "GTCAT": 104974, "GTCAC": 307132, "GTCAG": 38618, "GTCTA": 78970, "GTCTT": 204958, "GTCTC": 165515, "GTCTG": 340489, "GTCCA": 8869, "GTCCT": 146010, "GTCCC": 197138, "GTCCG": 6449, "GTCGA": 510733, "GTCGT": 687477, "GTCGC": 37922, "GTCGG": 388490, "GTGAA": 8557274, "GTGAT": 11662445, "GTGAC": 147389, "GTGAG": 4177625, "GTGTA": 12793035, "GTGTT": 16003943, "GTGTC": 237319, "GTGTG": 9359659, "GTGCA": 30712, "GTGCT": 47058, "GTGCC": 19307, "GTGCG": 209562, "GTGGA": 4389593, "GTGGT": 6286259, "GTGGC": 75185, "GTGGG": 2865972, "GCAAA": 45862, "GCAAT": 46704, "GCAAC": 6149, "GCAAG": 18523, "GCATA": 48350, "GCATT": 68223, "GCATC": 6086, "GCATG": 29260, "GCACA": 309719, "GCACT": 6269, "GCACC": 5334, "GCACG": 2858, "GCAGA": 21877, "GCAGT": 29085, "GCAGC": 3411, "GCAGG": 13473, "GCTAA": 34288, 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24651, "AGGGC": 17846, "AGGGG": 147230, "TAAAA": 84772964, "TAAAT": 48877405, "TAAAC": 25990450, "TAAAG": 82001, "TAATA": 48177643, "TAATT": 34415639, "TAATC": 15809155, "TAATG": 90103, "TAACA": 27587014, "TAACT": 15743479, "TAACC": 10897748, "TAACG": 1183684, "TAAGA": 60227, "TAAGT": 45671, "TAAGC": 20059, "TAAGG": 11167, "TATAA": 53878548, "TATAT": 48128345, "TATAC": 23423195, "TATAG": 61999, "TATTA": 36237139, "TATTT": 38338501, "TATTC": 14258130, "TATTG": 64445, "TATCA": 21679579, "TATCT": 15421025, "TATCC": 7395188, "TATCG": 894236, "TATGA": 103934, "TATGT": 79328, "TATGC": 30843, "TATGG": 4069, "TACAA": 30486341, "TACAT": 24718728, "TACAC": 13153895, "TACAG": 28346, "TACTA": 17706296, "TACTT": 13230872, "TACTC": 6156464, "TACTG": 33662, "TACCA": 10608307, "TACCT": 6999938, "TACCC": 4247974, "TACCG": 505246, "TACGA": 1112368, "TACGT": 807636, "TACGC": 417521, "TACGG": 2308, "TAGAA": 70283, "TAGAT": 304798, "TAGAC": 26817, "TAGAG": 12336, "TAGTA": 48533, "TAGTT": 49619, "TAGTC": 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2386024, "TCCCG": 218302, "TCCGA": 702600, "TCCGT": 437628, "TCCGC": 196450, "TCCGG": 1683, "TCGAA": 1421696, "TCGAT": 924227, "TCGAC": 500570, "TCGAG": 3204, "TCGTA": 814184, "TCGTT": 517914, "TCGTC": 350929, "TCGTG": 298407, "TCGCA": 376928, "TCGCT": 318831, "TCGCC": 486760, "TCGCG": 109159, "TCGGA": 22373, "TCGGT": 289266, "TCGGC": 1910, "TCGGG": 6380, "TGAAA": 167113, "TGAAT": 91832, "TGAAC": 48875, "TGAAG": 9502, "TGATA": 102918, "TGATT": 68933, "TGATC": 36519, "TGATG": 3645, "TGACA": 56762, "TGACT": 30137, "TGACC": 27378, "TGACG": 4207, "TGAGA": 11900, "TGAGT": 3407, "TGAGC": 2319, "TGAGG": 16247, "TGTAA": 83751, "TGTAT": 75803, "TGTAC": 32283, "TGTAG": 804263, "TGTTA": 52356, "TGTTT": 67015, "TGTTC": 38769, "TGTTG": 5226, "TGTCA": 45259, "TGTCT": 28241, "TGTCC": 20006, "TGTCG": 73387, "TGTGA": 3677, "TGTGT": 6553, "TGTGC": 5903, "TGTGG": 7949, "TGCAA": 39634, "TGCAT": 33380, "TGCAC": 18222, "TGCAG": 3055, "TGCTA": 24292, "TGCTT": 21039, "TGCTC": 25142, "TGCTG": 2541, "TGCCA": 15918, "TGCCT": 11138, "TGCCC": 9225, "TGCCG": 9064, "TGCGA": 3030, "TGCGT": 2599, "TGCGC": 2048, "TGCGG": 1452, "TGGAA": 15229, "TGGAT": 3768, "TGGAC": 2346, "TGGAG": 24222, "TGGTA": 2595, "TGGTT": 12636, "TGGTC": 287901, "TGGTG": 4568, "TGGCA": 2648, "TGGCT": 2389, "TGGCC": 2110, "TGGCG": 1672, "TGGGA": 29849, "TGGGT": 3777, "TGGGC": 2364, "TGGGG": 43841, "CAAAA": 50561412, "CAAAT": 29678472, "CAAAC": 16830528, "CAAAG": 57590, "CAATA": 30472958, "CAATT": 19225290, "CAATC": 10175371, "CAATG": 61206, "CAACA": 16785525, "CAACT": 9641194, "CAACC": 6048721, "CAACG": 492656, "CAAGA": 42469, "CAAGT": 30456, "CAAGC": 14866, "CAAGG": 4279, "CATAA": 25872139, "CATAT": 23483831, "CATAC": 11940496, "CATAG": 31078, "CATTA": 19624145, "CATTT": 19185682, "CATTC": 7390686, "CATTG": 33935, "CATCA": 12084133, "CATCT": 7241910, "CATCC": 4158824, "CATCG": 398827, "CATGA": 57185, "CATGT": 48164, "CATGC": 19766, "CATGG": 2915, "CACAA": 16921542, "CACAT": 11664279, "CACAC": 9776307, "CACAG": 21314, 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"CGTTC": 277300, "CGTTG": 3238, "CGTCA": 477493, "CGTCT": 358711, "CGTCC": 223443, "CGTCG": 431773, "CGTGA": 3766, "CGTGT": 585410, "CGTGC": 2017, "CGTGG": 3022, "CGCAA": 503927, "CGCAT": 331546, "CGCAC": 209012, "CGCAG": 29813, "CGCTA": 419058, "CGCTT": 293761, "CGCTC": 191246, "CGCTG": 2003, "CGCCA": 266607, "CGCCT": 160715, "CGCCC": 155180, "CGCCG": 351455, "CGCGA": 178440, "CGCGT": 379118, "CGCGC": 82101, "CGCGG": 1545, "CGGAA": 25313, "CGGAT": 2735, "CGGAC": 2242, "CGGAG": 2642, "CGGTA": 2378, "CGGTT": 3558, "CGGTC": 2174, "CGGTG": 287950, "CGGCA": 1835, "CGGCT": 1416, "CGGCC": 1569, "CGGCG": 1920, "CGGGA": 6731, "CGGGT": 1226, "CGGGC": 1080, "CGGGG": 3336, "GAAAA": 1935329, "GAAAT": 1070179, "GAAAC": 593578, "GAAAG": 468923, "GAATA": 964934, "GAATT": 705654, "GAATC": 446207, "GAATG": 12161, "GAACA": 543125, "GAACT": 340300, "GAACC": 262787, "GAACG": 123153, "GAAGA": 411837, "GAAGT": 37724, "GAAGC": 32182, "GAAGG": 172079, "GATAA": 1022347, "GATAT": 759450, "GATAC": 419672, "GATAG": 10350, "GATTA": 617232, "GATTT": 555533, "GATTC": 273479, "GATTG": 3736, "GATCA": 416883, "GATCT": 630909, "GATCC": 174676, "GATCG": 98966, "GATGA": 11422, "GATGT": 4578, "GATGC": 2652, "GATGG": 9580, "GACAA": 521392, "GACAT": 321450, "GACAC": 199836, "GACAG": 4019, "GACTA": 351359, "GACTT": 238436, "GACTC": 124462, "GACTG": 2411, "GACCA": 247778, "GACCT": 145644, "GACCC": 126523, "GACCG": 61469, "GACGA": 111089, "GACGT": 72119, "GACGC": 45231, "GACGG": 2133, "GAGAA": 422566, "GAGAT": 229546, "GAGAC": 109680, "GAGAG": 250360, "GAGTA": 49158, "GAGTT": 35139, "GAGTC": 26198, "GAGTG": 292406, "GAGCA": 38321, "GAGCT": 32127, "GAGCC": 23901, "GAGCG": 134232, "GAGGA": 307171, "GAGGT": 30178, "GAGGC": 25174, "GAGGG": 181623, "GTAAA": 726584, "GTAAT": 427056, "GTAAC": 281332, "GTAAG": 6478, "GTATA": 499630, "GTATT": 379479, "GTATC": 477010, "GTATG": 3649, "GTACA": 283099, "GTACT": 163540, "GTACC": 114686, "GTACG": 73113, "GTAGA": 262570, "GTAGT": 7921, "GTAGC": 259905, "GTAGG": 293027, "GTTAA": 384444, "GTTAT": 324748, "GTTAC": 166896, "GTTAG": 2572, "GTTTA": 322205, "GTTTT": 290993, "GTTTC": 162121, "GTTTG": 4746, "GTTCA": 187765, "GTTCT": 119861, "GTTCC": 84248, "GTTCG": 30733, "GTTGA": 3714, "GTTGT": 7564, "GTTGC": 10036, "GTTGG": 4016, "GTCAA": 270222, "GTCAT": 249698, "GTCAC": 151503, "GTCAG": 2606, "GTCTA": 210797, "GTCTT": 135479, "GTCTC": 103211, "GTCTG": 2653, "GTCCA": 103463, "GTCCT": 80522, "GTCCC": 65364, "GTCCG": 56633, "GTCGA": 94310, "GTCGT": 359196, "GTCGC": 336313, "GTCGG": 7089, "GTGAA": 6797, "GTGAT": 3648, "GTGAC": 2411, "GTGAG": 6026, "GTGTA": 807788, "GTGTT": 6472, "GTGTC": 74101, "GTGTG": 8186, "GTGCA": 2559, "GTGCT": 9251, "GTGCC": 1800, "GTGCG": 1356, "GTGGA": 6909, "GTGGT": 297322, "GTGGC": 2272, "GTGGG": 13340, "GCAAA": 345962, "GCAAT": 215251, "GCAAC": 140340, "GCAAG": 3645, "GCATA": 208779, "GCATT": 147752, "GCATC": 90279, "GCATG": 6662, "GCACA": 120529, "GCACT": 83787, "GCACC": 51874, "GCACG": 37386, "GCAGA": 4486, "GCAGT": 3644, 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/mmfs1/gscratch/scrubbed/sr320/chil-wgbs/Qui4 (paired)_R2.fastq.gz -o output/13-wgbs-hapa/trimmed/Qui4_R1.fq.gz -O output/13-wgbs-hapa/trimmed/Qui4_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs-hapa/fastp/Qui4.fastp.json --html output/13-wgbs-hapa/fastp/Qui4.fastp.html " }, "RP1 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 84335388, "total_bases": 12734643588, "q20_bases": 12585490360, "q30_bases": 12188219019, "q20_rate": 0.988288, "q30_rate": 0.957091, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.210136 }, "after_filtering": { "total_reads": 83039660, "total_bases": 11528495410, "q20_bases": 11428900502, "q30_bases": 11108538555, "q20_rate": 0.991361, "q30_rate": 0.963572, "read1_mean_length": 138, "read2_mean_length": 138, "gc_content": 0.187954 } }, "filtering_result": { 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295235, "CCTCT": 593884, "CCTCC": 835728, "CCTCG": 11889, "CCTGA": 35200, "CCTGT": 38673, "CCTGC": 19645, "CCTGG": 26641, "CCCAA": 101475, "CCCAT": 49225, "CCCAC": 60612, "CCCAG": 1430825, "CCCTA": 227751, "CCCTT": 334745, "CCCTC": 597225, "CCCTG": 28287, "CCCCA": 907541, "CCCCT": 384246, "CCCCC": 665191, "CCCCG": 17131, "CCCGA": 18298, "CCCGT": 18798, "CCCGC": 8712, "CCCGG": 8794, "CCGAA": 23462, "CCGAT": 74603, "CCGAC": 8505, "CCGAG": 13728, "CCGTA": 14179, "CCGTT": 65568, "CCGTC": 170076, "CCGTG": 13138, "CCGCA": 8359, "CCGCT": 11091, "CCGCC": 10594, "CCGCG": 4181, "CCGGA": 13952, "CCGGT": 14083, "CCGGC": 10143, "CCGGG": 9867, "CGAAA": 1002630, "CGAAT": 968066, "CGAAC": 60102, "CGAAG": 457133, "CGATA": 1291414, "CGATT": 1527054, "CGATC": 181681, "CGATG": 550203, "CGACA": 10806, "CGACT": 14994, "CGACC": 9397, "CGACG": 197219, "CGAGA": 611533, "CGAGT": 714074, "CGAGC": 46222, "CGAGG": 320611, "CGTAA": 1126287, "CGTAT": 1504734, "CGTAC": 111102, "CGTAG": 536400, "CGTTA": 1623797, 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6419, "CACGA": 11973, "CACGT": 260958, "CACGC": 6221, "CACGG": 7855, "CAGAA": 87362, "CAGAT": 95689, "CAGAC": 16430, "CAGAG": 49230, "CAGTA": 77631, "CAGTT": 130583, "CAGTC": 258771, "CAGTG": 60578, "CAGCA": 18571, "CAGCT": 18997, "CAGCC": 17039, "CAGCG": 5650, "CAGGA": 44244, "CAGGT": 56469, "CAGGC": 15141, "CAGGG": 34315, "CTAAA": 167915, "CTAAT": 142710, "CTAAC": 26931, "CTAAG": 52195, "CTATA": 181336, "CTATT": 227020, "CTATC": 34514, "CTATG": 88858, "CTACA": 23847, "CTACT": 35472, "CTACC": 18376, "CTACG": 8548, "CTAGA": 68687, "CTAGT": 96263, "CTAGC": 9507, "CTAGG": 48958, "CTTAA": 164430, "CTTAT": 189792, "CTTAC": 30936, "CTTAG": 69559, "CTTTA": 249516, "CTTTT": 1015504, "CTTTC": 453697, "CTTTG": 174909, "CTTCA": 87558, "CTTCT": 550416, "CTTCC": 638964, "CTTCG": 21298, "CTTGA": 105745, "CTTGT": 150004, "CTTGC": 19834, "CTTGG": 63781, "CTCAA": 72621, "CTCAT": 44887, "CTCAC": 39270, "CTCAG": 33408, "CTCTA": 47677, "CTCTT": 469744, "CTCTC": 754408, "CTCTG": 33796, "CTCCA": 272473, 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5068133, "GACAA": 57178, "GACAT": 68288, "GACAC": 11890, "GACAG": 36670, "GACTA": 44747, "GACTT": 98230, "GACTC": 42545, "GACTG": 31543, "GACCA": 16922, "GACCT": 32695, "GACCC": 40280, "GACCG": 4562, "GACGA": 461894, "GACGT": 671723, "GACGC": 27143, "GACGG": 354427, "GAGAA": 8355472, "GAGAT": 9227956, "GAGAC": 118684, "GAGAG": 10176776, "GAGTA": 7538064, "GAGTT": 10662185, "GAGTC": 151659, "GAGTG": 6042255, "GAGCA": 142170, "GAGCT": 41230, "GAGCC": 23262, "GAGCG": 249702, "GAGGA": 4405051, "GAGGT": 5034263, "GAGGC": 67779, "GAGGG": 2567338, "GTAAA": 21288476, "GTAAT": 22194180, "GTAAC": 232112, "GTAAG": 8157298, "GTATA": 28367920, "GTATT": 34027892, "GTATC": 481183, "GTATG": 13900606, "GTACA": 77102, "GTACT": 84976, "GTACC": 31408, "GTACG": 647401, "GTAGA": 9874341, "GTAGT": 11892932, "GTAGC": 127514, "GTAGG": 5169787, "GTTAA": 21735822, "GTTAT": 32721524, "GTTAC": 364357, "GTTAG": 11067199, "GTTTA": 30703789, "GTTTT": 53389362, "GTTTC": 822619, "GTTTG": 19038885, "GTTCA": 149803, "GTTCT": 286058, "GTTCC": 155759, "GTTCG": 1008737, "GTTGA": 12810859, "GTTGT": 18014770, "GTTGC": 168221, "GTTGG": 6451238, "GTCAA": 111364, "GTCAT": 136892, "GTCAC": 258929, "GTCAG": 53310, "GTCTA": 100220, "GTCTT": 327624, "GTCTC": 144286, "GTCTG": 301164, "GTCCA": 16296, "GTCCT": 129552, "GTCCC": 151877, "GTCCG": 8609, "GTCGA": 641317, "GTCGT": 1154120, "GTCGC": 48519, "GTCGG": 483310, "GTGAA": 10716690, "GTGAT": 13808907, "GTGAC": 180615, "GTGAG": 5005806, "GTGTA": 15215394, "GTGTT": 18692089, "GTGTC": 314944, "GTGTG": 10783715, "GTGCA": 45218, "GTGCT": 58846, "GTGCC": 48642, "GTGCG": 262761, "GTGGA": 5244979, "GTGGT": 7283519, "GTGGC": 123911, "GTGGG": 3347633, "GCAAA": 68107, "GCAAT": 65378, "GCAAC": 12667, "GCAAG": 29153, "GCATA": 66262, "GCATT": 94571, "GCATC": 15016, "GCATG": 42349, "GCACA": 262614, "GCACT": 14485, "GCACC": 11121, "GCACG": 5355, "GCAGA": 36053, "GCAGT": 44101, "GCAGC": 11409, "GCAGG": 23809, "GCTAA": 48892, "GCTAT": 69445, "GCTAC": 10027, "GCTAG": 25802, 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"GGTAT": 12748452, "GGTAC": 147516, "GGTAG": 4938349, "GGTTA": 12916239, "GGTTT": 18249432, "GGTTC": 342680, "GGTTG": 6636900, "GGTCA": 65162, "GGTCT": 101024, "GGTCC": 68557, "GGTCG": 449163, "GGTGA": 5478185, "GGTGT": 7771730, "GGTGC": 73856, "GGTGG": 3944824, "GGCAA": 31195, "GGCAT": 40262, "GGCAC": 9172, "GGCAG": 25495, "GGCTA": 28789, "GGCTT": 63912, "GGCTC": 31625, "GGCTG": 25247, "GGCCA": 12429, "GGCCT": 30228, "GGCCC": 30709, "GGCCG": 9353, "GGCGA": 260271, "GGCGT": 359837, "GGCGC": 29992, "GGCGG": 227161, "GGGAA": 4515776, "GGGAT": 5426195, "GGGAC": 81545, "GGGAG": 2544182, "GGGTA": 5138310, "GGGTT": 8301127, "GGGTC": 160815, "GGGTG": 3438492, "GGGCA": 24582, "GGGCT": 32852, "GGGCC": 22025, "GGGCG": 221888, "GGGGA": 2954498, "GGGGT": 4661424, "GGGGC": 95990, "GGGGG": 3378881 }, "overrepresented_sequences": {} }, "read2_after_filtering": { "total_reads": 41519830, "total_bases": 5764308050, "q20_bases": 5713048128, "q30_bases": 5550154721, "q40_bases": 5550154721, "total_cycles": 141, "quality_curves": { "A": [ 39.6857, 39.6979, 39.6839, 39.6422, 39.708, 39.6369, 39.6165, 39.6164, 39.6302, 39.6685, 39.6045, 39.6233, 39.6458, 39.6112, 39.5813, 39.6266, 39.6313, 39.6771, 39.6122, 39.5749, 39.6276, 39.5769, 39.6253, 39.5896, 39.6171, 39.5985, 39.5936, 39.6571, 39.6105, 39.5978, 39.5648, 39.5513, 39.5503, 39.5622, 39.6241, 39.581, 39.5495, 39.4702, 39.5941, 39.5392, 39.5131, 39.575, 39.5715, 39.5629, 39.5457, 39.5288, 39.5185, 39.5454, 39.5458, 39.5099, 39.5551, 39.4905, 39.4729, 39.4867, 39.4889, 39.4969, 39.4421, 39.3421, 39.3971, 39.3786, 39.2875, 39.3921, 39.3663, 39.4382, 39.314, 39.2238, 39.398, 39.3898, 39.4274, 39.3791, 39.3516, 39.3769, 39.3211, 39.2163, 39.2989, 39.3725, 39.2869, 39.3891, 39.3052, 39.2832, 39.2426, 39.273, 39.2906, 39.2593, 39.2386, 39.247, 39.2526, 39.2187, 39.1859, 39.2881, 39.2794, 39.1944, 39.2097, 39.2479, 39.1659, 39.235, 39.1258, 38.9298, 39.2121, 39.1554, 39.1389, 39.1243, 39.0848, 39.045, 39.0224, 39.0966, 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"TCCTG": 29737, "TCCCA": 5211055, "TCCCT": 3796976, "TCCCC": 2900999, "TCCCG": 277492, "TCCGA": 887002, "TCCGT": 570478, "TCCGC": 246999, "TCCGG": 3206, "TCGAA": 1792515, "TCGAT": 1156893, "TCGAC": 621029, "TCGAG": 5220, "TCGTA": 1046374, "TCGTT": 686107, "TCGTC": 448380, "TCGTG": 243869, "TCGCA": 441216, "TCGCT": 408766, "TCGCC": 486118, "TCGCG": 140904, "TCGGA": 30197, "TCGGT": 238176, "TCGGC": 3919, "TCGGG": 6943, "TGAAA": 227298, "TGAAT": 128907, "TGAAC": 79875, "TGAAG": 24448, "TGATA": 139559, "TGATT": 99330, "TGATC": 51256, "TGATG": 14737, "TGACA": 78435, "TGACT": 45329, "TGACC": 39335, "TGACG": 6549, "TGAGA": 31834, "TGAGT": 13162, "TGAGC": 11094, "TGAGG": 33524, "TGTAA": 109495, "TGTAT": 107241, "TGTAC": 46509, "TGTAG": 462353, "TGTTA": 75569, "TGTTT": 104157, "TGTTC": 50943, "TGTTG": 15390, "TGTCA": 55562, "TGTCT": 284371, "TGTCC": 30641, "TGTCG": 26153, "TGTGA": 15686, "TGTGT": 22863, "TGTGC": 14809, "TGTGG": 17830, "TGCAA": 58967, "TGCAT": 50693, "TGCAC": 29100, "TGCAG": 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"CTTCT": 6904131, "CTTCC": 4350420, "CTTCG": 403642, "CTTGA": 48483, "CTTGT": 31232, "CTTGC": 18213, "CTTGG": 237310, "CTCAA": 11539145, "CTCAT": 7233996, "CTCAC": 5058256, "CTCAG": 29194, "CTCTA": 10209490, "CTCTT": 6497257, "CTCTC": 9289916, "CTCTG": 38543, "CTCCA": 5135898, "CTCCT": 3690174, "CTCCC": 2566575, "CTCCG": 284291, "CTCGA": 792602, "CTCGT": 415889, "CTCGC": 283900, "CTCGG": 239654, "CTGAA": 80276, "CTGAT": 53505, "CTGAC": 30646, "CTGAG": 30566, "CTGTA": 55169, "CTGTT": 44598, "CTGTC": 29779, "CTGTG": 15511, "CTGCA": 32535, "CTGCT": 24916, "CTGCC": 18264, "CTGCG": 3891, "CTGGA": 38170, "CTGGT": 9376, "CTGGC": 10165, "CTGGG": 72363, "CCAAA": 20460540, "CCAAT": 12126856, "CCAAC": 6821163, "CCAAG": 31093, "CCATA": 12636156, "CCATT": 9433531, "CCATC": 5074748, "CCATG": 39912, "CCACA": 7396316, "CCACT": 4666148, "CCACC": 4185728, "CCACG": 277846, "CCAGA": 41895, "CCAGT": 22663, "CCAGC": 21449, "CCAGG": 15069, "CCTAA": 11308664, "CCTAT": 9620878, "CCTAC": 5138591, "CCTAG": 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"overrepresented_sequences": {} }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/RP1 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/RP1 (paired)_R2.fastq.gz -o output/13-wgbs-hapa/trimmed/Rio1_R1.fq.gz -O output/13-wgbs-hapa/trimmed/Rio1_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs-hapa/fastp/Rio1.fastp.json --html output/13-wgbs-hapa/fastp/Rio1.fastp.html " }, "RP2 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 72867540, "total_bases": 11002998540, "q20_bases": 10863709254, "q30_bases": 10504969712, "q20_rate": 0.987341, "q30_rate": 0.954737, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.213688 }, "after_filtering": { "total_reads": 71563070, "total_bases": 9897659364, "q20_bases": 9809433916, "q30_bases": 9532551151, "q20_rate": 0.991086, 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9748904, "TAGTA": 20133584, "TAGTT": 26425658, "TAGTC": 364055, "TAGTG": 12747096, "TAGCA": 52574, "TAGCT": 71649, "TAGCC": 31197, "TAGCG": 487732, "TAGGA": 7833358, "TAGGT": 11221290, "TAGGC": 114453, "TAGGG": 5629447, "TTAAA": 50048984, "TTAAT": 51289659, "TTAAC": 515051, "TTAAG": 18006274, "TTATA": 60435909, "TTATT": 82360273, "TTATC": 1100704, "TTATG": 28351234, "TTACA": 178851, "TTACT": 245729, "TTACC": 84075, "TTACG": 1341206, "TTAGA": 20784743, "TTAGT": 25862353, "TTAGC": 275894, "TTAGG": 10719859, "TTTAA": 60401964, "TTTAT": 86973241, "TTTAC": 950880, "TTTAG": 28448590, "TTTTA": 93164642, "TTTTT": 171537072, "TTTTC": 3180706, "TTTTG": 55002154, "TTTCA": 673988, "TTTCT": 1392956, "TTTCC": 998093, "TTTCG": 2678801, "TTTGA": 35273567, "TTTGT": 50972924, "TTTGC": 460621, "TTTGG": 19110477, "TTCAA": 440181, "TTCAT": 406443, "TTCAC": 105618, "TTCAG": 230794, "TTCTA": 360133, "TTCTT": 1132052, "TTCTC": 902008, "TTCTG": 198948, "TTCCA": 214587, "TTCCT": 765404, "TTCCC": 1089894, 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33883, "CATCG": 13144, "CATGA": 72133, "CATGT": 124936, "CATGC": 7626, "CATGG": 43664, "CACAA": 32011, "CACAT": 36005, "CACAC": 2082024, "CACAG": 15394, "CACTA": 23486, "CACTT": 84275, "CACTC": 45893, "CACTG": 13083, "CACCA": 39997, "CACCT": 27880, "CACCC": 46897, "CACCG": 9244, "CACGA": 13728, "CACGT": 1954775, "CACGC": 9601, "CACGG": 1288099, "CAGAA": 76977, "CAGAT": 2386729, "CAGAC": 13939, "CAGAG": 39597, "CAGTA": 69092, "CAGTT": 124223, "CAGTC": 1400443, "CAGTG": 48145, "CAGCA": 8357, "CAGCT": 13026, "CAGCC": 8539, "CAGCG": 4283, "CAGGA": 32702, "CAGGT": 51958, "CAGGC": 4422, "CAGGG": 28791, "CTAAA": 160415, "CTAAT": 134920, "CTAAC": 34461, "CTAAG": 47943, "CTATA": 177146, "CTATT": 223431, "CTATC": 1203535, "CTATG": 82292, "CTACA": 25066, "CTACT": 37634, "CTACC": 23873, "CTACG": 8812, "CTAGA": 446083, "CTAGT": 77617, "CTAGC": 7885, "CTAGG": 43392, "CTTAA": 158205, "CTTAT": 188319, "CTTAC": 36286, "CTTAG": 121513, "CTTTA": 236068, "CTTTT": 1039963, "CTTTC": 480882, "CTTTG": 178737, 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"CCTTT": 390302, "CCTTC": 478109, "CCTTG": 24157, "CCTCA": 279268, "CCTCT": 549146, "CCTCC": 814024, "CCTCG": 8896, "CCTGA": 21182, "CCTGT": 22281, "CCTGC": 7212, "CCTGG": 9368, "CCCAA": 82498, "CCCAT": 37398, "CCCAC": 45754, "CCCAG": 1444047, "CCCTA": 233487, "CCCTT": 306885, "CCCTC": 580570, "CCCTG": 13633, "CCCCA": 917400, "CCCCT": 373578, "CCCCC": 657640, "CCCCG": 13988, "CCCGA": 16134, "CCCGT": 15102, "CCCGC": 5853, "CCCGG": 6681, "CCGAA": 20778, "CCGAT": 67420, "CCGAC": 6906, "CCGAG": 10092, "CCGTA": 11632, "CCGTT": 24455, "CCGTC": 19116, "CCGTG": 8025, "CCGCA": 6031, "CCGCT": 5785, "CCGCC": 5565, "CCGCG": 2494, "CCGGA": 11722, "CCGGT": 9251, "CCGGC": 3807, "CCGGG": 5907, "CGAAA": 845455, "CGAAT": 840177, "CGAAC": 49979, "CGAAG": 389216, "CGATA": 1086964, "CGATT": 1309825, "CGATC": 1206001, "CGATG": 468843, "CGACA": 8060, "CGACT": 12541, "CGACC": 7991, "CGACG": 158995, "CGAGA": 519651, "CGAGT": 603307, "CGAGC": 33757, "CGAGG": 270875, "CGTAA": 949514, "CGTAT": 1281598, "CGTAC": 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105412, "GAAGG": 3692051, "GATAA": 17055207, "GATAT": 25532485, "GATAC": 253091, "GATAG": 9536567, "GATTA": 18022547, "GATTT": 31645923, "GATTC": 492615, "GATTG": 11193435, "GATCA": 72766, "GATCT": 1250521, "GATCC": 129350, "GATCG": 3405107, "GATGA": 8809230, "GATGT": 12986112, "GATGC": 105398, "GATGG": 4713009, "GACAA": 50041, "GACAT": 60027, "GACAC": 11047, "GACAG": 27838, "GACTA": 40740, "GACTT": 92153, "GACTC": 39248, "GACTG": 26586, "GACCA": 13796, "GACCT": 28028, "GACCC": 36334, "GACCG": 6604, "GACGA": 417536, "GACGT": 623822, "GACGC": 26217, "GACGG": 320119, "GAGAA": 7806710, "GAGAT": 8661236, "GAGAC": 106381, "GAGAG": 9595707, "GAGTA": 7047094, "GAGTT": 10032309, "GAGTC": 140104, "GAGTG": 5732506, "GAGCA": 2264438, "GAGCT": 34976, "GAGCC": 21983, "GAGCG": 224004, "GAGGA": 4144654, "GAGGT": 4735039, "GAGGC": 59307, "GAGGG": 2821360, "GTAAA": 19669592, "GTAAT": 20750013, "GTAAC": 212663, "GTAAG": 7673561, "GTATA": 26628598, "GTATT": 31975662, "GTATC": 449823, "GTATG": 13016502, "GTACA": 72176, "GTACT": 80068, "GTACC": 28521, "GTACG": 596693, "GTAGA": 9248587, "GTAGT": 11234282, "GTAGC": 116915, "GTAGG": 4823662, "GTTAA": 20241276, "GTTAT": 30863651, "GTTAC": 337565, "GTTAG": 10450744, "GTTTA": 28767742, "GTTTT": 50691920, "GTTTC": 792928, "GTTTG": 18124944, "GTTCA": 140777, "GTTCT": 297652, "GTTCC": 162137, "GTTCG": 932191, "GTTGA": 11983896, "GTTGT": 17013814, "GTTGC": 154310, "GTTGG": 6087103, "GTCAA": 104238, "GTCAT": 123336, "GTCAC": 1345101, "GTCAG": 47886, "GTCTA": 102397, "GTCTT": 246219, "GTCTC": 146742, "GTCTG": 1829719, "GTCCA": 14257, "GTCCT": 133926, "GTCCC": 164126, "GTCCG": 9610, "GTCGA": 597295, "GTCGT": 786969, "GTCGC": 42378, "GTCGG": 448401, "GTGAA": 9816840, "GTGAT": 13029240, "GTGAC": 166121, "GTGAG": 4676249, "GTGTA": 14367444, "GTGTT": 17886864, "GTGTC": 260954, "GTGTG": 10188307, "GTGCA": 37417, "GTGCT": 55573, "GTGCC": 19073, "GTGCG": 244565, "GTGGA": 4885896, "GTGGT": 6847961, "GTGGC": 92421, "GTGGG": 3220149, "GCAAA": 60847, "GCAAT": 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317587, "TCATC": 38421, "TCATG": 109303, "TCACA": 17518, "TCACT": 58614, "TCACC": 29853, "TCACG": 347998, "TCAGA": 81571, "TCAGT": 113651, "TCAGC": 7200, "TCAGG": 48779, "TCTAA": 161641, "TCTAT": 255758, "TCTAC": 24655, "TCTAG": 89333, "TCTTA": 201255, "TCTTT": 851948, "TCTTC": 639201, "TCTTG": 143330, "TCTCA": 44101, "TCTCT": 693686, "TCTCC": 987573, "TCTCG": 23836, "TCTGA": 417230, "TCTGT": 143973, "TCTGC": 9463, "TCTGG": 378932, "TCCAA": 32121, "TCCAT": 22451, "TCCAC": 15263, "TCCAG": 341868, "TCCTA": 32791, "TCCTT": 485065, "TCCTC": 867884, "TCCTG": 19708, "TCCCA": 60259, "TCCCT": 555230, "TCCCC": 1001731, "TCCCG": 7342, "TCCGA": 16766, "TCCGT": 19159, "TCCGC": 4362, "TCCGG": 7986, "TCGAA": 956920, "TCGAT": 1708706, "TCGAC": 68743, "TCGAG": 692935, "TCGTA": 1210032, "TCGTT": 1881270, "TCGTC": 119485, "TCGTG": 690531, "TCGCA": 7682, "TCGCT": 11121, "TCGCC": 5010, "TCGCG": 184300, "TCGGA": 687686, "TCGGT": 885663, "TCGGC": 46407, "TCGGG": 470675, "TGAAA": 22420257, "TGAAT": 20122309, 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44854, "CTGCA": 5696, "CTGCT": 8667, "CTGCC": 3749, "CTGCG": 2835, "CTGGA": 29917, "CTGGT": 40585, "CTGGC": 2895, "CTGGG": 351827, "CCAAA": 60940, "CCAAT": 20150, "CCAAC": 29204, "CCAAG": 10990, "CCATA": 13210, "CCATT": 26728, "CCATC": 14644, "CCATG": 6793, "CCACA": 11053, "CCACT": 25478, "CCACC": 19918, "CCACG": 4775, "CCAGA": 23560, "CCAGT": 340668, "CCAGC": 3624, "CCAGG": 5995, "CCTAA": 20910, "CCTAT": 19387, "CCTAC": 11931, "CCTAG": 8052, "CCTTA": 22185, "CCTTT": 310882, "CCTTC": 410672, "CCTTG": 15982, "CCTCA": 33917, "CCTCT": 481375, "CCTCC": 709555, "CCTCG": 4343, "CCTGA": 10751, "CCTGT": 14851, "CCTGC": 3750, "CCTGG": 6062, "CCCAA": 44255, "CCCAT": 19333, "CCCAC": 24509, "CCCAG": 24982, "CCCTA": 16198, "CCCTT": 248822, "CCCTC": 500256, "CCCTG": 7224, "CCCCA": 47492, "CCCCT": 303348, "CCCCC": 522941, "CCCCG": 5314, "CCCGA": 6178, "CCCGT": 6586, "CCCGC": 2253, "CCCGG": 2753, "CCGAA": 11420, "CCGAT": 11784, "CCGAC": 3265, "CCGAG": 4196, "CCGTA": 6801, "CCGTT": 16499, "CCGTC": 7016, "CCGTG": 5578, "CCGCA": 1735, "CCGCT": 2464, "CCGCC": 2017, "CCGCG": 1533, "CCGGA": 5308, "CCGGT": 6148, "CCGGC": 1208, "CCGGG": 3754, "CGAAA": 762993, "CGAAT": 764015, "CGAAC": 38975, "CGAAG": 349181, "CGATA": 994277, "CGATT": 1191824, "CGATC": 417344, "CGATG": 426981, "CGACA": 5435, "CGACT": 8113, "CGACC": 4324, "CGACG": 143683, "CGAGA": 469360, "CGAGT": 553523, "CGAGC": 29107, "CGAGG": 244865, "CGTAA": 870160, "CGTAT": 1173401, "CGTAC": 81270, "CGTAG": 420043, "CGTTA": 1266085, "CGTTT": 1801607, "CGTTC": 130332, "CGTTG": 501451, "CGTCA": 13712, "CGTCT": 355297, "CGTCC": 12994, "CGTCG": 206629, "CGTGA": 390382, "CGTGT": 680077, "CGTGC": 33388, "CGTGG": 231435, "CGCAA": 5300, "CGCAT": 6724, "CGCAC": 2613, "CGCAG": 2678, "CGCTA": 5262, "CGCTT": 10703, "CGCTC": 5658, "CGCTG": 3036, "CGCCA": 1469, "CGCCT": 4216, "CGCCC": 4594, "CGCCG": 1222, "CGCGA": 115227, "CGCGT": 158111, "CGCGC": 16498, "CGCGG": 80548, "CGGAA": 457447, "CGGAT": 628420, "CGGAC": 63944, "CGGAG": 240287, "CGGTA": 538519, "CGGTT": 760454, "CGGTC": 63463, "CGGTG": 291541, "CGGCA": 2446, "CGGCT": 340623, "CGGCC": 2390, "CGGCG": 82662, "CGGGA": 240530, "CGGGT": 364665, "CGGGC": 28658, "CGGGG": 232447, "GAAAA": 18935477, "GAAAT": 21169400, "GAAAC": 195113, "GAAAG": 7472667, "GAATA": 15189385, "GAATT": 20008657, "GAATC": 267405, "GAATG": 7696699, "GAACA": 46400, "GAACT": 396491, "GAACC": 24418, "GAACG": 296616, "GAAGA": 7195297, "GAAGT": 8277910, "GAAGC": 93366, "GAAGG": 3231481, "GATAA": 15666691, "GATAT": 23485866, "GATAC": 229933, "GATAG": 8736938, "GATTA": 16548168, "GATTT": 29125488, "GATTC": 446918, "GATTG": 10259814, "GATCA": 60108, "GATCT": 475845, "GATCC": 113584, "GATCG": 546238, "GATGA": 8089418, "GATGT": 11903353, "GATGC": 95345, "GATGG": 4310021, "GACAA": 41989, "GACAT": 51488, "GACAC": 4876, "GACAG": 23315, "GACTA": 34026, "GACTT": 77027, "GACTC": 30069, "GACTG": 22096, "GACCA": 8977, "GACCT": 21508, "GACCC": 29302, "GACCG": 3024, "GACGA": 380138, "GACGT": 569859, "GACGC": 21099, 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24893, "TCATA": 19600965, "TCATT": 17282878, "TCATC": 8053568, "TCATG": 36701, "TCACA": 11677271, "TCACT": 8784200, "TCACC": 4751158, "TCACG": 375891, "TCAGA": 25328, "TCAGT": 24877, "TCAGC": 9031, "TCAGG": 1839, "TCTAA": 19055907, "TCTAT": 17361907, "TCTAC": 8489485, "TCTAG": 30508, "TCTTA": 12794937, "TCTTT": 12859352, "TCTTC": 7033413, "TCTTG": 21501, "TCTCA": 7629362, "TCTCT": 10826360, "TCTCC": 3785662, "TCTCG": 761886, "TCTGA": 51910, "TCTGT": 37015, "TCTGC": 13175, "TCTGG": 66129, "TCCAA": 11098002, "TCCAT": 8221867, "TCCAC": 4535889, "TCCAG": 14263, "TCCTA": 7005569, "TCCTT": 5610838, "TCCTC": 3559369, "TCCTG": 13980, "TCCCA": 4433695, "TCCCT": 3238211, "TCCCC": 2495676, "TCCCG": 229431, "TCCGA": 736574, "TCCGT": 466951, "TCCGC": 206275, "TCCGG": 1363, "TCGAA": 1516927, "TCGAT": 1016695, "TCGAC": 526055, "TCGAG": 2442, "TCGTA": 978160, "TCGTT": 571996, "TCGTC": 381241, "TCGTG": 355386, "TCGCA": 369774, "TCGCT": 341974, "TCGCC": 528175, "TCGCG": 112382, "TCGGA": 24092, "TCGGT": 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372221, "ACGTA": 905154, "ACGTT": 1238350, "ACGTC": 2156880, "ACGTG": 434245, "ACGCA": 6833, "ACGCT": 14104, "ACGCC": 6326, "ACGCG": 109383, "ACGGA": 519926, "ACGGT": 494690, "ACGGC": 32552, "ACGGG": 228322, "AGAAA": 16890338, "AGAAT": 14740367, "AGAAC": 157403, "AGAAG": 6327012, "AGATA": 17167316, "AGATT": 17303322, "AGATC": 3615862, "AGATG": 7586808, "AGACA": 53021, "AGACT": 54633, "AGACC": 27364, "AGACG": 404742, "AGAGA": 13100422, "AGAGT": 7954161, "AGAGC": 2706215, "AGAGG": 4027660, "AGTAA": 13749968, "AGTAT": 18437008, "AGTAC": 196778, "AGTAG": 7199197, "AGTTA": 16307813, "AGTTT": 26068981, "AGTTC": 398349, "AGTTG": 9524282, "AGTCA": 1497967, "AGTCT": 145676, "AGTCC": 105944, "AGTCG": 471183, "AGTGA": 8981907, "AGTGT": 12004151, "AGTGC": 101727, "AGTGG": 3964790, "AGCAA": 51669, "AGCAT": 55512, "AGCAC": 2446941, "AGCAG": 25569, "AGCTA": 36243, "AGCTT": 73499, "AGCTC": 46295, "AGCTG": 19907, "AGCCA": 12038, "AGCCT": 36648, "AGCCC": 47750, "AGCCG": 3011, "AGCGA": 370436, "AGCGT": 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"TCTCA": 180231, "TCTCT": 886723, "TCTCC": 1297639, "TCTCG": 29085, "TCTGA": 1985457, "TCTGT": 182239, "TCTGC": 27263, "TCTGG": 985117, "TCCAA": 53000, "TCCAT": 35159, "TCCAC": 27738, "TCCAG": 2200555, "TCCTA": 156514, "TCCTT": 614889, "TCCTC": 1133078, "TCCTG": 27524, "TCCCA": 808466, "TCCCT": 731209, "TCCCC": 1339232, "TCCCG": 13727, "TCCGA": 72498, "TCCGT": 29235, "TCCGC": 8762, "TCCGG": 11530, "TCGAA": 998366, "TCGAT": 1403308, "TCGAC": 75666, "TCGAG": 704070, "TCGTA": 1227767, "TCGTT": 1940156, "TCGTC": 159582, "TCGTG": 718635, "TCGCA": 10691, "TCGCT": 14766, "TCGCC": 7102, "TCGCG": 194435, "TCGGA": 3743781, "TCGGT": 910939, "TCGGC": 56459, "TCGGG": 492815, "TGAAA": 22898885, "TGAAT": 19955894, "TGAAC": 1979090, "TGAAG": 8529623, "TGATA": 22017163, "TGATT": 26991402, "TGATC": 470847, "TGATG": 12048200, "TGACA": 65490, "TGACT": 111567, "TGACC": 61465, "TGACG": 507708, "TGAGA": 7791129, "TGAGT": 8638273, "TGAGC": 110712, "TGAGG": 3870998, "TGTAA": 22374590, "TGTAT": 34737186, 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"CTACC": 18552, "CTACG": 7770, "CTAGA": 508640, "CTAGT": 101518, "CTAGC": 6650, "CTAGG": 160311, "CTTAA": 145800, "CTTAT": 174205, "CTTAC": 29692, "CTTAG": 126818, "CTTTA": 223445, "CTTTT": 1089786, "CTTTC": 517462, "CTTTG": 221235, "CTTCA": 152666, "CTTCT": 579474, "CTTCC": 843026, "CTTCG": 22534, "CTTGA": 95505, "CTTGT": 145889, "CTTGC": 14618, "CTTGG": 65003, "CTCAA": 64996, "CTCAT": 39470, "CTCAC": 33698, "CTCAG": 420381, "CTCTA": 127658, "CTCTT": 597672, "CTCTC": 931350, "CTCTG": 24615, "CTCCA": 2112197, "CTCCT": 688022, "CTCCC": 1134305, "CTCCG": 12484, "CTCGA": 17396, "CTCGT": 21011, "CTCGC": 5303, "CTCGG": 11472, "CTGAA": 1906036, "CTGAT": 75654, "CTGAC": 17426, "CTGAG": 32914, "CTGTA": 87760, "CTGTT": 152027, "CTGTC": 23684, "CTGTG": 53573, "CTGCA": 10526, "CTGCT": 22729, "CTGCC": 7850, "CTGCG": 3973, "CTGGA": 46191, "CTGGT": 154471, "CTGGC": 5280, "CTGGG": 818545, "CCAAA": 91083, "CCAAT": 32840, "CCAAC": 45231, "CCAAG": 23234, "CCATA": 21539, "CCATT": 38833, "CCATC": 28700, "CCATG": 9674, "CCACA": 28227, "CCACT": 36021, "CCACC": 33073, "CCACG": 12729, "CCAGA": 2306995, "CCAGT": 1508619, "CCAGC": 11734, "CCAGG": 14304, "CCTAA": 30742, "CCTAT": 27823, "CCTAC": 18787, "CCTAG": 355158, "CCTTA": 75369, "CCTTT": 400925, "CCTTC": 540867, "CCTTG": 22301, "CCTCA": 314702, "CCTCT": 640617, "CCTCC": 956200, "CCTCG": 7353, "CCTGA": 19336, "CCTGT": 19522, "CCTGC": 6228, "CCTGG": 9383, "CCCAA": 79990, "CCCAT": 31694, "CCCAC": 41146, "CCCAG": 1718242, "CCCTA": 264374, "CCCTT": 331484, "CCCTC": 674426, "CCCTG": 12137, "CCCCA": 1083396, "CCCCT": 419288, "CCCCC": 761074, "CCCCG": 12899, "CCCGA": 15665, "CCCGT": 13347, "CCCGC": 5007, "CCCGG": 4324, "CCGAA": 19593, "CCGAT": 64755, "CCGAC": 5264, "CCGAG": 10267, "CCGTA": 10639, "CCGTT": 28775, "CCGTC": 31079, "CCGTG": 7922, "CCGCA": 5770, "CCGCT": 5437, "CCGCC": 4894, "CCGCG": 3298, "CCGGA": 11963, "CCGGT": 10054, "CCGGC": 3543, "CCGGG": 5721, "CGAAA": 822272, "CGAAT": 773908, "CGAAC": 49984, "CGAAG": 366983, "CGATA": 1030194, "CGATT": 1206347, "CGATC": 153204, "CGATG": 441286, "CGACA": 8431, "CGACT": 11661, "CGACC": 7560, "CGACG": 152432, "CGAGA": 491537, "CGAGT": 563760, "CGAGC": 34637, "CGAGG": 253082, "CGTAA": 902160, "CGTAT": 1191413, "CGTAC": 87175, "CGTAG": 422975, "CGTTA": 1269856, "CGTTT": 1973852, "CGTTC": 141263, "CGTTG": 515218, "CGTCA": 23993, "CGTCT": 2118686, "CGTCC": 23444, "CGTCG": 219056, "CGTGA": 405791, "CGTGT": 733508, "CGTGC": 35447, "CGTGG": 238229, "CGCAA": 7707, "CGCAT": 8671, "CGCAC": 5652, "CGCAG": 6603, "CGCTA": 7000, "CGCTT": 15670, "CGCTC": 10222, "CGCTG": 6964, "CGCCA": 3548, "CGCCT": 8309, "CGCCC": 7176, "CGCCG": 1986, "CGCGA": 122486, "CGCGT": 166436, "CGCGC": 18979, "CGCGG": 86585, "CGGAA": 3417705, "CGGAT": 649187, "CGGAC": 70972, "CGGAG": 263126, "CGGTA": 556495, "CGGTT": 823909, "CGGTC": 73430, "CGGTG": 295017, "CGGCA": 1221802, "CGGCT": 35506, "CGGCC": 6116, "CGGCG": 86684, "CGGGA": 252828, "CGGGT": 395210, "CGGGC": 31015, "CGGGG": 245916, "GAAAA": 19969017, "GAAAT": 21411840, "GAAAC": 222772, "GAAAG": 7878706, "GAATA": 15394465, "GAATT": 19754643, "GAATC": 308027, "GAATG": 7804627, "GAACA": 60882, "GAACT": 1803494, "GAACC": 48245, "GAACG": 312803, "GAAGA": 10120429, "GAAGT": 8289452, "GAAGC": 95527, "GAAGG": 3370157, "GATAA": 15845773, "GATAT": 23193670, "GATAC": 238089, "GATAG": 8867290, "GATTA": 16333409, "GATTT": 28243518, "GATTC": 512348, "GATTG": 10156061, "GATCA": 72740, "GATCT": 264815, "GATCC": 209394, "GATCG": 3790086, "GATGA": 8132128, "GATGT": 11771231, "GATGC": 102482, "GATGG": 4328900, "GACAA": 51025, "GACAT": 60803, "GACAC": 12629, "GACAG": 29130, "GACTA": 38950, "GACTT": 96737, "GACTC": 57843, "GACTG": 25705, "GACCA": 12438, "GACCT": 42368, "GACCC": 57974, "GACCG": 6428, "GACGA": 406278, "GACGT": 581820, "GACGC": 24768, "GACGG": 310397, "GAGAA": 7465640, "GAGAT": 7964813, "GAGAC": 105520, "GAGAG": 9975203, "GAGTA": 6517846, "GAGTT": 9019104, "GAGTC": 143993, "GAGTG": 5478211, "GAGCA": 2539267, "GAGCT": 38026, "GAGCC": 29152, "GAGCG": 217673, "GAGGA": 3853083, "GAGGT": 4253821, "GAGGC": 60699, "GAGGG": 2504113, "GTAAA": 18382904, "GTAAT": 19026768, "GTAAC": 211054, "GTAAG": 7093833, "GTATA": 24429619, "GTATT": 28581509, "GTATC": 453551, "GTATG": 11773245, "GTACA": 72986, "GTACT": 82231, "GTACC": 33901, "GTACG": 563287, "GTAGA": 8570628, "GTAGT": 10182869, "GTAGC": 111866, "GTAGG": 4394212, "GTTAA": 18382757, "GTTAT": 27580764, "GTTAC": 323537, "GTTAG": 9399189, "GTTTA": 25760979, "GTTTT": 44985398, "GTTTC": 757700, "GTTTG": 16187648, "GTTCA": 137654, "GTTCT": 300233, "GTTCC": 178986, "GTTCG": 855565, "GTTGA": 10826236, "GTTGT": 15243023, "GTTGC": 150373, "GTTGG": 5488695, "GTCAA": 101570, "GTCAT": 125838, "GTCAC": 1418761, "GTCAG": 48949, "GTCTA": 98833, "GTCTT": 342611, "GTCTC": 167754, "GTCTG": 1992075, "GTCCA": 16380, "GTCCT": 152598, "GTCCC": 192563, "GTCCG": 9462, "GTCGA": 556733, "GTCGT": 823390, "GTCGC": 41071, "GTCGG": 436539, "GTGAA": 9093431, "GTGAT": 11963866, "GTGAC": 162909, "GTGAG": 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42821167, "TTAAT": 42935143, "TTAAC": 452277, "TTAAG": 14969387, "TTATA": 50519678, "TTATT": 67858832, "TTATC": 986690, "TTATG": 23372406, "TTACA": 156737, "TTACT": 207004, "TTACC": 74142, "TTACG": 1154936, "TTAGA": 17477339, "TTAGT": 21341845, "TTAGC": 241416, "TTAGG": 8794017, "TTTAA": 50845934, "TTTAT": 71997965, "TTTAC": 818303, "TTTAG": 23604853, "TTTTA": 77455505, "TTTTT": 139768104, "TTTTC": 2700100, "TTTTG": 44900299, "TTTCA": 534699, "TTTCT": 1214914, "TTTCC": 935693, "TTTCG": 2258414, "TTTGA": 29257443, "TTTGT": 41855487, "TTTGC": 390364, "TTTGG": 15243576, "TTCAA": 353452, "TTCAT": 343065, "TTCAC": 65821, "TTCAG": 131916, "TTCTA": 274747, "TTCTT": 970712, "TTCTC": 910073, "TTCTG": 163969, "TTCCA": 45578, "TTCCT": 732093, "TTCCC": 1104283, "TTCCG": 34013, "TTCGA": 1437548, "TTCGT": 1804471, "TTCGC": 93214, "TTCGG": 971974, "TTGAA": 22090538, "TTGAT": 27242390, "TTGAC": 291857, "TTGAG": 8870261, "TTGTA": 27824873, "TTGTT": 39463170, "TTGTC": 555024, "TTGTG": 14924500, "TTGCA": 83937, "TTGCT": 133104, "TTGCC": 50904, "TTGCG": 491401, "TTGGA": 9947479, "TTGGT": 13587162, "TTGGC": 145168, "TTGGG": 5923559, "TCAAA": 269302, "TCAAT": 215284, "TCAAC": 55789, "TCAAG": 81943, "TCATA": 188815, "TCATT": 311070, "TCATC": 35831, "TCATG": 109288, "TCACA": 326654, "TCACT": 52163, "TCACC": 27047, "TCACG": 12728, "TCAGA": 82934, "TCAGT": 111098, "TCAGC": 6930, "TCAGG": 47404, "TCTAA": 152452, "TCTAT": 240896, "TCTAC": 23206, "TCTAG": 118983, "TCTTA": 188575, "TCTTT": 888425, "TCTTC": 752554, "TCTTG": 136349, "TCTCA": 40592, "TCTCT": 807161, "TCTCC": 1187164, "TCTCG": 22023, "TCTGA": 389959, "TCTGT": 143108, "TCTGC": 11959, "TCTGG": 322137, "TCCAA": 29561, "TCCAT": 22611, "TCCAC": 15247, "TCCAG": 318166, "TCCTA": 30829, "TCCTT": 538792, "TCCTC": 1032358, "TCCTG": 17870, "TCCCA": 57653, "TCCCT": 660294, "TCCCC": 1198326, "TCCCG": 6570, "TCCGA": 16439, "TCCGT": 18587, "TCCGC": 4620, "TCCGG": 7672, "TCGAA": 895369, "TCGAT": 1279997, "TCGAC": 67293, "TCGAG": 641393, "TCGTA": 1124225, "TCGTT": 1732715, "TCGTC": 121211, "TCGTG": 651017, "TCGCA": 7655, "TCGCT": 10780, "TCGCC": 4990, "TCGCG": 177084, "TCGGA": 652375, "TCGGT": 822492, "TCGGC": 45234, "TCGGG": 439074, "TGAAA": 20975360, "TGAAT": 18424874, "TGAAC": 488906, "TGAAG": 7813696, "TGATA": 20341162, "TGATT": 24888576, "TGATC": 430634, "TGATG": 11093830, "TGACA": 56231, "TGACT": 95565, "TGACC": 53354, "TGACG": 464339, "TGAGA": 7115841, "TGAGT": 7950656, "TGAGC": 100644, "TGAGG": 3541176, "TGTAA": 20653497, "TGTAT": 31970017, "TGTAC": 309707, "TGTAG": 10475642, "TGTTA": 25076980, "TGTTT": 40473243, "TGTTC": 564313, "TGTTG": 14650623, "TGTCA": 124507, "TGTCT": 240792, "TGTCC": 148071, "TGTCG": 630666, "TGTGA": 10561090, "TGTGT": 18077581, "TGTGC": 130859, "TGTGG": 5524230, "TGCAA": 60104, "TGCAT": 81699, "TGCAC": 6039, "TGCAG": 31769, "TGCTA": 55542, "TGCTT": 117407, "TGCTC": 47688, "TGCTG": 33782, "TGCCA": 5716, "TGCCT": 37944, "TGCCC": 47118, "TGCCG": 5531, "TGCGA": 357377, "TGCGT": 457320, "TGCGC": 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6779, "CCCCA": 49837, "CCCCT": 355479, "CCCCC": 627804, "CCCCG": 4782, "CCCGA": 5902, "CCCGT": 5985, "CCCGC": 2155, "CCCGG": 2352, "CCGAA": 11094, "CCGAT": 11976, "CCGAC": 2715, "CCGAG": 4132, "CCGTA": 6640, "CCGTT": 16856, "CCGTC": 8968, "CCGTG": 5356, "CCGCA": 1591, "CCGCT": 2415, "CCGCC": 1966, "CCGCG": 1560, "CCGGA": 5314, "CCGGT": 6087, "CCGGC": 1475, "CCGGG": 3590, "CGAAA": 742803, "CGAAT": 710863, "CGAAC": 38626, "CGAAG": 328760, "CGATA": 945520, "CGATT": 1105739, "CGATC": 92190, "CGATG": 404680, "CGACA": 5551, "CGACT": 8539, "CGACC": 5078, "CGACG": 138242, "CGAGA": 444747, "CGAGT": 517424, "CGAGC": 30224, "CGAGG": 229158, "CGTAA": 827200, "CGTAT": 1089177, "CGTAC": 78341, "CGTAG": 386384, "CGTTA": 1164436, "CGTTT": 1706273, "CGTTC": 121491, "CGTTG": 466724, "CGTCA": 13578, "CGTCT": 355202, "CGTCC": 13876, "CGTCG": 195330, "CGTGA": 368886, "CGTGT": 650282, "CGTGC": 31260, "CGTGG": 216105, "CGCAA": 5430, "CGCAT": 6546, "CGCAC": 2663, "CGCAG": 2793, "CGCTA": 5157, "CGCTT": 10588, 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42668, "GACAT": 52888, "GACAC": 5042, "GACAG": 24520, "GACTA": 33385, "GACTT": 82884, "GACTC": 48067, "GACTG": 21426, "GACCA": 8209, "GACCT": 36353, "GACCC": 50569, "GACCG": 2880, "GACGA": 370399, "GACGT": 529148, "GACGC": 20278, "GACGG": 281364, "GAGAA": 6786975, "GAGAT": 7283559, "GAGAC": 92435, "GAGAG": 9052154, "GAGTA": 5982728, "GAGTT": 8265910, "GAGTC": 126733, "GAGTG": 4984417, "GAGCA": 174901, "GAGCT": 32880, "GAGCC": 18702, "GAGCG": 197839, "GAGGA": 3496238, "GAGGT": 3871309, "GAGGC": 48516, "GAGGG": 2013888, "GTAAA": 16924510, "GTAAT": 17482297, "GTAAC": 190823, "GTAAG": 6497548, "GTATA": 22389614, "GTATT": 26222230, "GTATC": 414175, "GTATG": 10779968, "GTACA": 62844, "GTACT": 72936, "GTACC": 30033, "GTACG": 513304, "GTAGA": 7858654, "GTAGT": 9327265, "GTAGC": 101662, "GTAGG": 4012146, "GTTAA": 16882142, "GTTAT": 25323019, "GTTAC": 292789, "GTTAG": 8621862, "GTTTA": 23701960, "GTTTT": 41078288, "GTTTC": 672617, "GTTTG": 14719479, "GTTCA": 115783, "GTTCT": 253910, "GTTCC": 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--trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs-hapa/fastp/Rio3.fastp.json --html output/13-wgbs-hapa/fastp/Rio3.fastp.html " }, "RP4 (paired)_R1": { "summary": { "fastp_version": "1.0.1", "sequencing": "paired end (151 cycles + 151 cycles)", "before_filtering": { "total_reads": 81176642, "total_bases": 12257672942, "q20_bases": 12113072958, "q30_bases": 11721362139, "q20_rate": 0.988203, "q30_rate": 0.956247, "read1_mean_length": 151, "read2_mean_length": 151, "gc_content": 0.211639 }, "after_filtering": { "total_reads": 79945874, "total_bases": 11077433848, "q20_bases": 10982648499, "q30_bases": 10671919288, "q20_rate": 0.991443, "q30_rate": 0.963393, "read1_mean_length": 138, "read2_mean_length": 138, "gc_content": 0.188384 } }, "filtering_result": { "passed_filter_reads": 79945874, "low_quality_reads": 614450, "too_many_N_reads": 1462, "too_short_reads": 614856, "too_long_reads": 0 }, "duplication": { "rate": 0.043348 }, 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19577914, "TAAGC": 207050, "TAAGG": 8725286, "TATAA": 49368787, "TATAT": 81531189, "TATAC": 746352, "TATAG": 27457717, "TATTA": 60486629, "TATTT": 103927361, "TATTC": 1278984, "TATTG": 37265524, "TATCA": 265409, "TATCT": 502557, "TATCC": 320569, "TATCG": 1772878, "TATGA": 23660014, "TATGT": 38447739, "TATGC": 308274, "TATGG": 13011818, "TACAA": 178629, "TACAT": 262246, "TACAC": 35193, "TACAG": 107733, "TACTA": 153221, "TACTT": 296596, "TACTC": 96418, "TACTG": 94702, "TACCA": 41418, "TACCT": 79435, "TACCC": 92161, "TACCG": 11419, "TACGA": 1129315, "TACGT": 1530653, "TACGC": 75147, "TACGG": 692042, "TAGAA": 19774784, "TAGAT": 24870451, "TAGAC": 309525, "TAGAG": 10728207, "TAGTA": 22237654, "TAGTT": 29320582, "TAGTC": 428298, "TAGTG": 13886177, "TAGCA": 74863, "TAGCT": 96300, "TAGCC": 44144, "TAGCG": 562140, "TAGGA": 8807246, "TAGGT": 12472356, "TAGGC": 135010, "TAGGG": 6237673, "TTAAA": 56603172, "TTAAT": 57427704, "TTAAC": 615636, "TTAAG": 19982189, "TTATA": 67271444, "TTATT": 91901050, "TTATC": 1286076, "TTATG": 31444878, "TTACA": 241319, "TTACT": 303541, "TTACC": 102282, "TTACG": 1541286, "TTAGA": 23384256, "TTAGT": 28622845, "TTAGC": 332123, "TTAGG": 11902152, "TTTAA": 67777873, "TTTAT": 96981094, "TTTAC": 1111859, "TTTAG": 31806156, "TTTTA": 104109903, "TTTTT": 191296334, "TTTTC": 3617514, "TTTTG": 61406675, "TTTCA": 825834, "TTTCT": 1596752, "TTTCC": 1125114, "TTTCG": 3066877, "TTTGA": 39494758, "TTTGT": 56886665, "TTTGC": 540878, "TTTGG": 21360237, "TTCAA": 542823, "TTCAT": 518170, "TTCAC": 129627, "TTCAG": 292354, "TTCTA": 430334, "TTCTT": 1291393, "TTCTC": 1043457, "TTCTG": 252922, "TTCCA": 267031, "TTCCT": 879478, "TTCCC": 1229186, "TTCCG": 102406, "TTCGA": 1944445, "TTCGT": 2453206, "TTCGC": 130963, "TTCGG": 1326239, "TTGAA": 29399199, "TTGAT": 36755266, "TTGAC": 377519, "TTGAG": 11975318, "TTGTA": 37236383, "TTGTT": 53826216, "TTGTC": 742305, "TTGTG": 20141756, "TTGCA": 137664, "TTGCT": 195150, "TTGCC": 72397, "TTGCG": 658232, "TTGGA": 13682726, "TTGGT": 18667302, "TTGGC": 202822, "TTGGG": 8502792, "TCAAA": 418945, "TCAAT": 326045, "TCAAC": 104849, "TCAAG": 136182, "TCATA": 284110, "TCATT": 480250, "TCATC": 74690, "TCATG": 169463, "TCACA": 1270596, "TCACT": 105862, "TCACC": 65676, "TCACG": 23580, "TCAGA": 624447, "TCAGT": 183802, "TCAGC": 26490, "TCAGG": 83389, "TCTAA": 240187, "TCTAT": 360709, "TCTAC": 50510, "TCTAG": 264108, "TCTTA": 315566, "TCTTT": 1191560, "TCTTC": 906911, "TCTTG": 225595, "TCTCA": 210512, "TCTCT": 925556, "TCTCC": 1279157, "TCTCG": 37873, "TCTGA": 1868975, "TCTGT": 227061, "TCTGC": 33599, "TCTGG": 980035, "TCCAA": 74219, "TCCAT": 65441, "TCCAC": 43750, "TCCAG": 2041302, "TCCTA": 170179, "TCCTT": 660556, "TCCTC": 1127560, "TCCTG": 50955, "TCCCA": 806193, "TCCCT": 728454, "TCCCC": 1283872, "TCCCG": 18633, "TCCGA": 81101, "TCCGT": 35522, "TCCGC": 11035, "TCCGG": 15022, "TCGAA": 1214594, "TCGAT": 1726479, "TCGAC": 94769, "TCGAG": 1947535, "TCGTA": 1509810, "TCGTT": 2362443, "TCGTC": 161618, "TCGTG": 866228, "TCGCA": 14039, "TCGCT": 19401, "TCGCC": 9850, "TCGCG": 243357, "TCGGA": 3710316, "TCGGT": 1112564, "TCGGC": 66078, "TCGGG": 610690, "TGAAA": 27388596, "TGAAT": 24397637, "TGAAC": 1872493, "TGAAG": 10281818, "TGATA": 26840373, "TGATT": 33643098, "TGATC": 495062, "TGATG": 14623007, "TGACA": 92384, "TGACT": 130632, "TGACC": 56944, "TGACG": 617929, "TGAGA": 9319179, "TGAGT": 10534958, "TGAGC": 145406, "TGAGG": 4826116, "TGTAA": 27257918, "TGTAT": 42263767, "TGTAC": 412586, "TGTAG": 13926253, "TGTTA": 33588794, "TGTTT": 55072509, "TGTTC": 754341, "TGTTG": 19871852, "TGTCA": 190484, "TGTCT": 329590, "TGTCC": 179350, "TGTCG": 831921, "TGTGA": 13922538, "TGTGT": 23541937, "TGTGC": 182939, "TGTGG": 7499974, "TGCAA": 101569, "TGCAT": 130785, "TGCAC": 21688, "TGCAG": 62138, "TGCTA": 89820, "TGCTT": 175338, "TGCTC": 67837, "TGCTG": 64421, "TGCCA": 22930, "TGCCT": 63450, "TGCCC": 65412, "TGCCG": 7067, "TGCGA": 472232, "TGCGT": 611979, "TGCGC": 39615, "TGCGG": 259176, "TGGAA": 11056577, "TGGAT": 12745665, 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1998163, "CACGC": 12800, "CACGG": 10851, "CAGAA": 114323, "CAGAT": 2671713, "CAGAC": 24581, "CAGAG": 64288, "CAGTA": 96635, "CAGTT": 167332, "CAGTC": 1337940, "CAGTG": 74255, "CAGCA": 24105, "CAGCT": 27222, "CAGCC": 24045, "CAGCG": 6897, "CAGGA": 55877, "CAGGT": 75868, "CAGGC": 19390, "CAGGG": 44741, "CTAAA": 202190, "CTAAT": 170733, "CTAAC": 42285, "CTAAG": 64620, "CTATA": 214200, "CTATT": 276401, "CTATC": 48579, "CTATG": 106617, "CTACA": 35363, "CTACT": 49608, "CTACC": 30039, "CTACG": 10920, "CTAGA": 497348, "CTAGT": 96646, "CTAGC": 13594, "CTAGG": 72054, "CTTAA": 197585, "CTTAT": 232468, "CTTAC": 45457, "CTTAG": 146721, "CTTTA": 294037, "CTTTT": 1178571, "CTTTC": 561032, "CTTTG": 223322, "CTTCA": 185785, "CTTCT": 554777, "CTTCC": 851605, "CTTCG": 28583, "CTTGA": 126438, "CTTGT": 188869, "CTTGC": 26511, "CTTGG": 81452, "CTCAA": 95023, "CTCAT": 62916, "CTCAC": 55686, "CTCAG": 425493, "CTCTA": 144354, "CTCTT": 632304, "CTCTC": 934838, "CTCTG": 45000, "CTCCA": 1959352, "CTCCT": 698001, "CTCCC": 1095328, "CTCCG": 15185, "CTCGA": 24674, "CTCGT": 26842, "CTCGC": 8339, "CTCGG": 16499, "CTGAA": 1783755, "CTGAT": 103692, "CTGAC": 28940, "CTGAG": 56257, "CTGTA": 121487, "CTGTT": 184596, "CTGTC": 40464, "CTGTG": 81600, "CTGCA": 27659, "CTGCT": 30198, "CTGCC": 22910, "CTGCG": 6617, "CTGGA": 66381, "CTGGT": 77062, "CTGGC": 17340, "CTGGG": 911090, "CCAAA": 124240, "CCAAT": 47888, "CCAAC": 61602, "CCAAG": 34825, "CCATA": 34983, "CCATT": 72549, "CCATC": 44514, "CCATG": 24249, "CCACA": 41749, "CCACT": 59620, "CCACC": 54807, "CCACG": 15903, "CCAGA": 2190734, "CCAGT": 1368708, "CCAGC": 25742, "CCAGG": 29815, "CCTAA": 47260, "CCTAT": 42550, "CCTAC": 30404, "CCTAG": 335187, "CCTTA": 89621, "CCTTT": 453598, "CCTTC": 555203, "CCTTG": 38134, "CCTCA": 326298, "CCTCT": 644486, "CCTCC": 935056, "CCTCG": 13225, "CCTGA": 35668, "CCTGT": 37665, "CCTGC": 19882, "CCTGG": 26883, "CCCAA": 102183, "CCCAT": 50777, "CCCAC": 60452, "CCCAG": 1627946, "CCCTA": 254363, "CCCTT": 355367, "CCCTC": 661786, "CCCTG": 27197, "CCCCA": 1026331, "CCCCT": 418183, "CCCCC": 727042, "CCCCG": 16655, "CCCGA": 18915, "CCCGT": 17473, "CCCGC": 7987, "CCCGG": 8331, "CCGAA": 23417, "CCGAT": 72147, "CCGAC": 7835, "CCGAG": 14853, "CCGTA": 13798, "CCGTT": 29507, "CCGTC": 24244, "CCGTG": 11010, "CCGCA": 7901, "CCGCT": 7456, "CCGCC": 8445, "CCGCG": 3628, "CCGGA": 14514, "CCGGT": 11413, "CCGGC": 4702, "CCGGG": 9475, "CGAAA": 979768, "CGAAT": 945030, "CGAAC": 59831, "CGAAG": 442736, "CGATA": 1259502, "CGATT": 1492737, "CGATC": 1114098, "CGATG": 542099, "CGACA": 10577, "CGACT": 14827, "CGACC": 9611, "CGACG": 190506, "CGAGA": 596364, "CGAGT": 687662, "CGAGC": 1104022, "CGAGG": 312855, "CGTAA": 1090153, "CGTAT": 1450626, "CGTAC": 106044, "CGTAG": 520310, "CGTTA": 1568088, "CGTTT": 2283788, "CGTTC": 176922, "CGTTG": 639621, "CGTCA": 27813, "CGTCT": 1884735, "CGTCC": 25103, "CGTCG": 274119, "CGTGA": 490032, "CGTGT": 855371, "CGTGC": 45519, "CGTGG": 295126, "CGCAA": 10122, "CGCAT": 12110, "CGCAC": 7840, "CGCAG": 8959, "CGCTA": 9572, "CGCTT": 19611, "CGCTC": 14442, "CGCTG": 9666, "CGCCA": 6236, "CGCCT": 11732, "CGCCC": 10694, "CGCCG": 3277, "CGCGA": 151077, "CGCGT": 205627, "CGCGC": 25751, "CGCGG": 108045, "CGGAA": 3326115, "CGGAT": 798150, "CGGAC": 87806, "CGGAG": 315050, "CGGTA": 677271, "CGGTT": 966670, "CGGTC": 87916, "CGGTG": 357545, "CGGCA": 7605, "CGGCT": 15609, "CGGCC": 7321, "CGGCG": 108511, "CGGGA": 313895, "CGGGT": 473896, "CGGGC": 40094, "CGGGG": 310156, "GAAAA": 23524664, "GAAAT": 25838139, "GAAAC": 274283, "GAAAG": 9250909, "GAATA": 18561568, "GAATT": 24319626, "GAATC": 363516, "GAATG": 9382522, "GAACA": 83546, "GAACT": 1673081, "GAACC": 50345, "GAACG": 378651, "GAAGA": 11361453, "GAAGT": 10011386, "GAAGC": 122258, "GAAGG": 4094433, "GATAA": 19059786, "GATAT": 28354812, "GATAC": 292154, "GATAG": 10538222, "GATTA": 20127349, "GATTT": 35383714, "GATTC": 589615, "GATTG": 12497473, "GATCA": 99724, "GATCT": 1184830, "GATCC": 171652, "GATCG": 3727015, "GATGA": 9801892, "GATGT": 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11328830, "CAACC": 7137610, "CAACG": 596397, "CAAGA": 251709, "CAAGT": 54633, "CAAGC": 32365, "CAAGG": 22771, "CATAA": 30202686, "CATAT": 27885486, "CATAC": 13786034, "CATAG": 184507, "CATTA": 23752686, "CATTT": 23300564, "CATTC": 8942317, "CATTG": 63063, "CATCA": 14007535, "CATCT": 8685580, "CATCC": 4900906, "CATCG": 500917, "CATGA": 96521, "CATGT": 77366, "CATGC": 38483, "CATGG": 16240, "CACAA": 19561366, "CACAT": 13455638, "CACAC": 10658475, "CACAG": 93014, "CACTA": 13391579, "CACTT": 8845720, "CACTC": 5757059, "CACTG": 48752, "CACCA": 8285548, "CACCT": 5547232, "CACCC": 3867054, "CACCG": 352166, "CACGA": 790173, "CACGT": 494178, "CACGC": 260412, "CACGG": 6488, "CAGAA": 95016, "CAGAT": 549939, "CAGAC": 58042, "CAGAG": 36927, "CAGTA": 49697, "CAGTT": 42998, "CAGTC": 29291, "CAGTG": 34976, "CAGCA": 38530, "CAGCT": 28895, "CAGCC": 29739, "CAGCG": 5741, "CAGGA": 30566, "CAGGT": 14859, "CAGGC": 16049, "CAGGG": 26282, "CTAAA": 30467827, "CTAAT": 19427050, "CTAAC": 11122713, "CTAAG": 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227534, "GGCAC": 162865, "GGCAG": 32358, "GGCTA": 336247, "GGCTT": 188539, "GGCTC": 174936, "GGCTG": 19209, "GGCCA": 279978, "GGCCT": 147189, "GGCCC": 149257, "GGCCG": 27169, "GGCGA": 45840, "GGCGT": 21746, "GGCGC": 16870, "GGCGG": 25693, "GGGAA": 13794576, "GGGAT": 3782812, "GGGAC": 1876553, "GGGAG": 12232905, "GGGTA": 659583, "GGGTT": 432776, "GGGTC": 381357, "GGGTG": 137679, "GGGCA": 511547, "GGGCT": 409188, "GGGCC": 343296, "GGGCG": 53499, "GGGGA": 14071486, "GGGGT": 936162, "GGGGC": 768837, "GGGGG": 47112314 }, "overrepresented_sequences": {} }, "read1_after_filtering": { "total_reads": 39972937, "total_bases": 5538094657, "q20_bases": 5491797339, "q30_bases": 5336371017, "q40_bases": 5336371017, "total_cycles": 141, "quality_curves": { "A": [ 39.129, 39.2434, 39.26, 39.2626, 39.1258, 39.283, 39.2467, 39.3318, 39.2795, 39.3296, 39.3035, 39.4043, 39.407, 39.2897, 39.3338, 39.3497, 39.2747, 39.3503, 39.3023, 39.2594, 39.3121, 39.2764, 39.1716, 39.2001, 39.2908, 39.1906, 39.3872, 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19124, "ACCTA": 17174, "ACCTT": 70587, "ACCTC": 82339, "ACCTG": 17075, "ACCCA": 21066, "ACCCT": 96456, "ACCCC": 111282, "ACCCG": 6954, "ACCGA": 7836, "ACCGT": 11032, "ACCGC": 3202, "ACCGG": 6136, "ACGAA": 728711, "ACGAT": 963885, "ACGAC": 80822, "ACGAG": 411146, "ACGTA": 994311, "ACGTT": 1377167, "ACGTC": 366398, "ACGTG": 474634, "ACGCA": 7428, "ACGCT": 9227, "ACGCC": 5321, "ACGCG": 120816, "ACGGA": 569966, "ACGGT": 545463, "ACGGC": 33317, "ACGGG": 255338, "AGAAA": 18324754, "AGAAT": 16281471, "AGAAC": 166954, "AGAAG": 6903760, "AGATA": 18753083, "AGATT": 19443093, "AGATC": 316742, "AGATG": 8424983, "AGACA": 63793, "AGACT": 64400, "AGACC": 27491, "AGACG": 443112, "AGAGA": 12644240, "AGAGT": 8334518, "AGAGC": 233194, "AGAGG": 4284376, "AGTAA": 14986824, "AGTAT": 20542871, "AGTAC": 215155, "AGTAG": 7947642, "AGTTA": 18281809, "AGTTT": 29825733, "AGTTC": 439400, "AGTTG": 10824828, "AGTCA": 348850, "AGTCT": 146949, "AGTCC": 100475, "AGTCG": 519455, "AGTGA": 9493375, "AGTGT": 12707703, 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34308634, "TATCA": 233957, "TATCT": 450244, "TATCC": 294321, "TATCG": 1620882, "TATGA": 21799049, "TATGT": 35420114, "TATGC": 280702, "TATGG": 11956609, "TACAA": 155934, "TACAT": 230259, "TACAC": 23311, "TACAG": 94715, "TACTA": 131955, "TACTT": 254144, "TACTC": 80266, "TACTG": 82220, "TACCA": 29183, "TACCT": 64144, "TACCC": 76612, "TACCG": 8306, "TACGA": 1036032, "TACGT": 1400943, "TACGC": 67383, "TACGG": 633008, "TAGAA": 18173994, "TAGAT": 22419241, "TAGAC": 281474, "TAGAG": 9811420, "TAGTA": 20467436, "TAGTT": 26951040, "TAGTC": 389569, "TAGTG": 12734667, "TAGCA": 65748, "TAGCT": 84836, "TAGCC": 39898, "TAGCG": 513864, "TAGGA": 8063114, "TAGGT": 11430165, "TAGGC": 122306, "TAGGG": 5663119, "TTAAA": 52479046, "TTAAT": 53247965, "TTAAC": 554520, "TTAAG": 18475961, "TTATA": 62454661, "TTATT": 85271160, "TTATC": 1179563, "TTATG": 29113318, "TTACA": 211967, "TTACT": 263269, "TTACC": 83624, "TTACG": 1418556, "TTAGA": 21536962, "TTAGT": 26500158, "TTAGC": 304009, "TTAGG": 10970408, "TTTAA": 63063549, "TTTAT": 90355576, "TTTAC": 1012438, "TTTAG": 29523533, "TTTTA": 97208775, "TTTTT": 177359510, "TTTTC": 3264323, "TTTTG": 56576456, "TTTCA": 707956, "TTTCT": 1419635, "TTTCC": 999862, "TTTCG": 2823657, "TTTGA": 36518414, "TTTGT": 52663009, "TTTGC": 484671, "TTTGG": 19288408, "TTCAA": 468578, "TTCAT": 458372, "TTCAC": 94230, "TTCAG": 183670, "TTCTA": 362092, "TTCTT": 1125395, "TTCTC": 952989, "TTCTG": 215134, "TTCCA": 79649, "TTCCT": 785620, "TTCCC": 1113335, "TTCCG": 41586, "TTCGA": 1785088, "TTCGT": 2252964, "TTCGC": 117159, "TTCGG": 1212806, "TTGAA": 27102656, "TTGAT": 33944065, "TTGAC": 341699, "TTGAG": 10993666, "TTGTA": 34479077, "TTGTT": 49644396, "TTGTC": 675322, "TTGTG": 18582753, "TTGCA": 118951, "TTGCT": 168079, "TTGCC": 63597, "TTGCG": 602032, "TTGGA": 12404892, "TTGGT": 17149826, "TTGGC": 182339, "TTGGG": 7524089, "TCAAA": 357072, "TCAAT": 286295, "TCAAC": 78945, "TCAAG": 116167, "TCATA": 250990, "TCATT": 419327, "TCATC": 55193, "TCATG": 149343, "TCACA": 300559, "TCACT": 79301, "TCACC": 43975, "TCACG": 18320, "TCAGA": 117404, "TCAGT": 153236, "TCAGC": 19959, "TCAGG": 72407, "TCTAA": 203695, "TCTAT": 313567, "TCTAC": 35197, "TCTAG": 114868, "TCTTA": 251533, "TCTTT": 1012357, "TCTTC": 769507, "TCTTG": 186233, "TCTCA": 66192, "TCTCT": 842789, "TCTCC": 1177736, "TCTCG": 28971, "TCTGA": 382417, "TCTGT": 191761, "TCTGC": 23453, "TCTGG": 329595, "TCCAA": 48146, "TCCAT": 47932, "TCCAC": 28400, "TCCAG": 290914, "TCCTA": 45212, "TCCTT": 578568, "TCCTC": 1033638, "TCCTG": 38183, "TCCCA": 82279, "TCCCT": 659734, "TCCCC": 1157505, "TCCCG": 10545, "TCCGA": 20942, "TCCGT": 24822, "TCCGC": 6782, "TCCGG": 10664, "TCGAA": 1094458, "TCGAT": 1577476, "TCGAC": 84059, "TCGAG": 1056426, "TCGTA": 1384088, "TCGTT": 2153197, "TCGTC": 142735, "TCGTG": 788925, "TCGCA": 10202, "TCGCT": 14572, "TCGCC": 7130, "TCGCG": 221023, "TCGGA": 793366, "TCGGT": 1011847, "TCGGC": 56887, "TCGGG": 546445, "TGAAA": 25271142, "TGAAT": 22539693, "TGAAC": 491082, "TGAAG": 9442525, "TGATA": 24810768, "TGATT": 31039836, "TGATC": 451756, "TGATG": 13477742, "TGACA": 80917, "TGACT": 111786, "TGACC": 48556, "TGACG": 566898, "TGAGA": 8537201, "TGAGT": 9699149, "TGAGC": 132210, "TGAGG": 4414399, "TGTAA": 25179233, "TGTAT": 38965892, "TGTAC": 376125, "TGTAG": 12829713, "TGTTA": 31063297, "TGTTT": 50702086, "TGTTC": 685942, "TGTTG": 18311182, "TGTCA": 167315, "TGTCT": 289764, "TGTCC": 162610, "TGTCG": 756860, "TGTGA": 12825055, "TGTGT": 21645595, "TGTGC": 164798, "TGTGG": 6875012, "TGCAA": 87511, "TGCAT": 116003, "TGCAC": 15913, "TGCAG": 53747, "TGCTA": 78415, "TGCTT": 147784, "TGCTC": 60055, "TGCTG": 55039, "TGCCA": 18170, "TGCCT": 55151, "TGCCC": 58235, "TGCCG": 5064, "TGCGA": 432473, "TGCGT": 559736, "TGCGC": 35669, "TGCGG": 234459, "TGGAA": 10037162, "TGGAT": 11701419, "TGGAC": 134969, "TGGAG": 5009058, "TGGTA": 11811425, "TGGTT": 16338946, "TGGTC": 274312, "TGGTG": 7592316, "TGGCA": 44664, "TGGCT": 68923, "TGGCC": 38339, "TGGCG": 303483, "TGGGA": 5059059, "TGGGT": 6533257, 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5425, "CAGGA": 48259, "CAGGT": 61678, "CAGGC": 16646, "CAGGG": 37160, "CTAAA": 170429, "CTAAT": 146802, "CTAAC": 26475, "CTAAG": 54410, "CTATA": 184314, "CTATT": 234982, "CTATC": 33675, "CTATG": 92772, "CTACA": 24096, "CTACT": 35263, "CTACC": 18333, "CTACG": 8297, "CTAGA": 72066, "CTAGT": 81020, "CTAGC": 9959, "CTAGG": 42803, "CTTAA": 165886, "CTTAT": 196667, "CTTAC": 30985, "CTTAG": 73033, "CTTTA": 241070, "CTTTT": 963300, "CTTTC": 485082, "CTTTG": 176138, "CTTCA": 84954, "CTTCT": 489306, "CTTCC": 717307, "CTTCG": 20956, "CTTGA": 105138, "CTTGT": 156940, "CTTGC": 20434, "CTTGG": 64791, "CTCAA": 69425, "CTCAT": 44382, "CTCAC": 38420, "CTCAG": 34889, "CTCTA": 45691, "CTCTT": 513843, "CTCTC": 856334, "CTCTG": 33972, "CTCCA": 299704, "CTCCT": 630083, "CTCCC": 986267, "CTCCG": 8019, "CTCGA": 16116, "CTCGT": 19837, "CTCGC": 5392, "CTCGG": 10240, "CTGAA": 358924, "CTGAT": 85365, "CTGAC": 18652, "CTGAG": 44312, "CTGTA": 104914, "CTGTT": 151192, "CTGTC": 31396, "CTGTG": 69642, "CTGCA": 21122, "CTGCT": 23285, "CTGCC": 17518, "CTGCG": 4976, "CTGGA": 50060, "CTGGT": 58873, "CTGGC": 14212, "CTGGG": 301488, "CCAAA": 83727, "CCAAT": 31247, "CCAAC": 40197, "CCAAG": 22302, "CCATA": 22551, "CCATT": 52359, "CCATC": 27071, "CCATG": 19392, "CCACA": 24994, "CCACT": 41097, "CCACC": 35313, "CCACG": 7373, "CCAGA": 39065, "CCAGT": 283610, "CCAGC": 18051, "CCAGG": 21652, "CCTAA": 31512, "CCTAT": 28725, "CCTAC": 18737, "CCTAG": 15717, "CCTTA": 32301, "CCTTT": 373684, "CCTTC": 492915, "CCTTG": 28748, "CCTCA": 51768, "CCTCT": 583981, "CCTCC": 843216, "CCTCG": 7055, "CCTGA": 24107, "CCTGT": 28979, "CCTGC": 15502, "CCTGG": 21993, "CCCAA": 60090, "CCCAT": 31431, "CCCAC": 37445, "CCCAG": 44402, "CCCTA": 23968, "CCCTT": 299103, "CCCTC": 591372, "CCCTG": 19802, "CCCCA": 64237, "CCCCT": 354655, "CCCCC": 598560, "CCCCG": 7991, "CCCGA": 8586, "CCCGT": 9186, "CCCGC": 4683, "CCCGG": 5889, "CCGAA": 14191, "CCGAT": 15431, "CCGAC": 4643, "CCGAG": 7742, "CCGTA": 9031, "CCGTT": 21216, "CCGTC": 9860, "CCGTG": 8412, "CCGCA": 3497, "CCGCT": 4267, "CCGCC": 5207, "CCGCG": 2596, "CCGGA": 7510, "CCGGT": 8318, "CCGGC": 3424, "CCGGG": 7204, "CGAAA": 888168, "CGAAT": 864249, "CGAAC": 47249, "CGAAG": 399021, "CGATA": 1149884, "CGATT": 1363240, "CGATC": 370851, "CGATG": 492999, "CGACA": 7976, "CGACT": 10903, "CGACC": 6294, "CGACG": 172377, "CGAGA": 540532, "CGAGT": 630210, "CGAGC": 308712, "CGAGG": 283658, "CGTAA": 1000678, "CGTAT": 1330285, "CGTAC": 95635, "CGTAG": 476132, "CGTTA": 1439578, "CGTTT": 2072065, "CGTTC": 155593, "CGTTG": 580955, "CGTCA": 17838, "CGTCT": 294269, "CGTCC": 16077, "CGTCG": 246474, "CGTGA": 446238, "CGTGT": 773277, "CGTGC": 40539, "CGTGG": 268116, "CGCAA": 7377, "CGCAT": 9242, "CGCAC": 4226, "CGCAG": 5204, "CGCTA": 6941, "CGCTT": 14259, "CGCTC": 7739, "CGCTG": 5348, "CGCCA": 3924, "CGCCT": 7545, "CGCCC": 7530, "CGCCG": 2466, "CGCGA": 137330, "CGCGT": 187254, "CGCGC": 21534, "CGCGG": 97980, "CGGAA": 527103, "CGGAT": 728520, "CGGAC": 78498, "CGGAG": 280452, "CGGTA": 620897, "CGGTT": 865365, "CGGTC": 77452, "CGGTG": 325445, "CGGCA": 4503, "CGGCT": 9037, "CGGCC": 5731, "CGGCG": 98566, "CGGGA": 283850, "CGGGT": 417865, "CGGGC": 35733, "CGGGG": 271044, "GAAAA": 21622190, "GAAAT": 23864644, "GAAAC": 243166, "GAAAG": 8403756, "GAATA": 17100803, "GAATT": 22423633, "GAATC": 326303, "GAATG": 8623484, "GAACA": 68374, "GAACT": 353177, "GAACC": 34660, "GAACG": 345194, "GAAGA": 8073849, "GAAGT": 9185711, "GAAGC": 109076, "GAAGG": 3622603, "GATAA": 17531265, "GATAT": 26103268, "GATAC": 266571, "GATAG": 9662620, "GATTA": 18506308, "GATTT": 32584926, "GATTC": 538909, "GATTG": 11460501, "GATCA": 85255, "GATCT": 460765, "GATCC": 156101, "GATCG": 633713, "GATGA": 9008949, "GATGT": 13166007, "GATGC": 114843, "GATGG": 4869879, "GACAA": 61079, "GACAT": 74175, "GACAC": 12215, "GACAG": 40352, "GACTA": 47386, "GACTT": 103797, "GACTC": 46048, "GACTG": 33947, "GACCA": 17121, "GACCT": 35880, "GACCC": 43889, "GACCG": 4477, "GACGA": 450861, "GACGT": 646732, "GACGC": 26019, "GACGG": 343894, "GAGAA": 8009209, "GAGAT": 8811100, "GAGAC": 118094, "GAGAG": 9189496, "GAGTA": 7251422, "GAGTT": 10257900, "GAGTC": 153814, "GAGTG": 5464678, "GAGCA": 159834, "GAGCT": 43792, "GAGCC": 26541, "GAGCG": 508718, "GAGGA": 4232100, "GAGGT": 4838213, "GAGGC": 67713, "GAGGG": 2464573, "GTAAA": 20448821, "GTAAT": 21219710, "GTAAC": 231857, "GTAAG": 7850731, "GTATA": 26743155, "GTATT": 32610036, "GTATC": 487330, "GTATG": 13258413, "GTACA": 83363, "GTACT": 91465, "GTACC": 34444, "GTACG": 623050, "GTAGA": 9501110, "GTAGT": 11438722, "GTAGC": 129937, "GTAGG": 4939464, "GTTAA": 20787277, "GTTAT": 31393583, "GTTAC": 363387, "GTTAG": 10660842, "GTTTA": 29503227, "GTTTT": 51684747, "GTTTC": 826954, "GTTTG": 18450230, "GTTCA": 157554, "GTTCT": 304833, "GTTCC": 175962, "GTTCG": 973787, "GTTGA": 12327188, "GTTGT": 17447469, "GTTGC": 168358, "GTTGG": 6247833, "GTCAA": 116372, "GTCAT": 143702, "GTCAC": 284221, "GTCAG": 57149, "GTCTA": 101015, "GTCTT": 239682, "GTCTC": 164369, "GTCTG": 329590, "GTCCA": 16775, "GTCCT": 144939, "GTCCC": 177715, "GTCCG": 8170, "GTCGA": 619955, "GTCGT": 822535, "GTCGC": 46524, "GTCGG": 462956, "GTGAA": 9958493, "GTGAT": 13217278, "GTGAC": 179577, "GTGAG": 4680264, "GTGTA": 14351666, "GTGTT": 18017745, "GTGTC": 270090, "GTGTG": 9924417, "GTGCA": 48399, "GTGCT": 62001, "GTGCC": 24610, "GTGCG": 250814, "GTGGA": 5047685, "GTGGT": 7023032, "GTGGC": 96471, "GTGGG": 3223697, "GCAAA": 72347, "GCAAT": 71581, "GCAAC": 13133, "GCAAG": 31314, "GCATA": 71362, "GCATT": 102842, "GCATC": 12803, "GCATG": 46952, "GCACA": 290220, "GCACT": 15409, "GCACC": 11296, "GCACG": 5296, "GCAGA": 39446, "GCAGT": 47250, "GCAGC": 11381, "GCAGG": 26308, "GCTAA": 51555, "GCTAT": 73605, "GCTAC": 10421, "GCTAG": 27614, "GCTTA": 58638, "GCTTT": 152199, "GCTTC": 51547, "GCTTG": 42237, "GCTCA": 15695, "GCTCT": 58294, "GCTCC": 66834, "GCTCG": 5572, "GCTGA": 34671, "GCTGT": 46828, "GCTGC": 11480, "GCTGG": 27907, "GCCAA": 14140, "GCCAT": 12548, "GCCAC": 12658, "GCCAG": 12480, "GCCTA": 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4154, "CGCTA": 511012, "CGCTT": 356498, "CGCTC": 236053, "CGCTG": 3779, "CGCCA": 315258, "CGCCT": 192063, "CGCCC": 186864, "CGCCG": 341692, "CGCGA": 217469, "CGCGT": 120622, "CGCGC": 95763, "CGCGG": 1967, "CGGAA": 34581, "CGGAT": 4792, "CGGAC": 3589, "CGGAG": 4693, "CGGTA": 3755, "CGGTT": 4815, "CGGTC": 2912, "CGGTG": 260233, "CGGCA": 3331, "CGGCT": 3378, "CGGCC": 3493, "CGGCG": 2187, "CGGGA": 10166, "CGGGT": 2740, "CGGGC": 2985, "CGGGG": 5521, "GAAAA": 2586077, "GAAAT": 1448664, "GAAAC": 758663, "GAAAG": 596653, "GAATA": 1288352, "GAATT": 972157, "GAATC": 599773, "GAATG": 35854, "GAACA": 693779, "GAACT": 445708, "GAACC": 339245, "GAACG": 150488, "GAAGA": 686371, "GAAGT": 68302, "GAAGC": 55194, "GAAGG": 315410, "GATAA": 1344606, "GATAT": 1020958, "GATAC": 558718, "GATAG": 23063, "GATTA": 854509, "GATTT": 782799, "GATTC": 384184, "GATTG": 263070, "GATCA": 564782, "GATCT": 711595, "GATCC": 245538, "GATCG": 127239, "GATGA": 28976, "GATGT": 14379, "GATGC": 8763, "GATGG": 23925, "GACAA": 666006, "GACAT": 424454, "GACAC": 253832, "GACAG": 15562, "GACTA": 438233, "GACTT": 300711, "GACTC": 166588, "GACTG": 12353, "GACCA": 315592, "GACCT": 195853, "GACCC": 167241, "GACCG": 77701, "GACGA": 134888, "GACGT": 91716, "GACGC": 54468, "GACGG": 3992, "GAGAA": 748081, "GAGAT": 397169, "GAGAC": 176276, "GAGAG": 471945, "GAGTA": 83215, "GAGTT": 65191, "GAGTC": 45945, "GAGTG": 274861, "GAGCA": 64535, "GAGCT": 51932, "GAGCC": 44203, "GAGCG": 120924, "GAGGA": 548132, "GAGGT": 59403, "GAGGC": 52190, "GAGGG": 286135, "GTAAA": 922541, "GTAAT": 556586, "GTAAC": 346279, "GTAAG": 14432, "GTATA": 643600, "GTATT": 499087, "GTATC": 501378, "GTATG": 10619, "GTACA": 360323, "GTACT": 213465, "GTACC": 145512, "GTACG": 91285, "GTAGA": 480013, "GTAGT": 10601, "GTAGC": 6880, "GTAGG": 269327, "GTTAA": 500782, "GTTAT": 429737, "GTTAC": 222499, "GTTAG": 8249, "GTTTA": 419442, "GTTTT": 409576, "GTTTC": 222297, "GTTTG": 16060, "GTTCA": 223038, "GTTCT": 159579, "GTTCC": 107603, "GTTCG": 40403, "GTTGA": 12327, "GTTGT": 16857, "GTTGC": 13382, "GTTGG": 10913, "GTCAA": 329169, "GTCAT": 292660, "GTCAC": 185511, "GTCAG": 10603, "GTCTA": 268532, "GTCTT": 179619, "GTCTC": 130854, "GTCTG": 10692, "GTCCA": 135328, "GTCCT": 103844, "GTCCC": 85265, "GTCCG": 71819, "GTCGA": 132427, "GTCGT": 342218, "GTCGC": 284856, "GTCGG": 8587, "GTGAA": 20648, "GTGAT": 14330, "GTGAC": 9198, "GTGAG": 18303, "GTGTA": 489995, "GTGTT": 18078, "GTGTC": 50097, "GTGTG": 283472, "GTGCA": 11265, "GTGCT": 19442, "GTGCC": 8023, "GTGCG": 2602, "GTGGA": 18763, "GTGGT": 540348, "GTGGC": 10755, "GTGGG": 24075, "GCAAA": 439875, "GCAAT": 272843, "GCAAC": 162114, "GCAAG": 11874, "GCATA": 272861, "GCATT": 202184, "GCATC": 113527, "GCATG": 11444, "GCACA": 156230, "GCACT": 112695, "GCACC": 67777, "GCACG": 40060, "GCAGA": 16330, "GCAGT": 13118, "GCAGC": 9794, "GCAGG": 13711, "GCTAA": 309358, "GCTAT": 270072, "GCTAC": 134516, "GCTAG": 6181, "GCTTA": 190477, "GCTTT": 199298, "GCTTC": 110825, "GCTTG": 9421, "GCTCA": 139305, "GCTCT": 125287, "GCTCC": 63551, "GCTCG": 42258, "GCTGA": 13828, "GCTGT": 10950, "GCTGC": 9416, "GCTGG": 15043, "GCCAA": 192193, "GCCAT": 184964, "GCCAC": 98013, "GCCAG": 12209, "GCCTA": 127680, "GCCTT": 95000, "GCCTC": 67235, "GCCTG": 14671, "GCCCA": 100000, "GCCCT": 76113, "GCCCC": 76868, "GCCCG": 36643, "GCCGA": 60988, "GCCGT": 278137, "GCCGC": 22404, "GCCGG": 3073, "GCGAA": 112503, "GCGAT": 79112, "GCGAC": 45135, "GCGAG": 3724, "GCGTA": 65347, "GCGTT": 42314, "GCGTC": 290849, "GCGTG": 4237, "GCGCA": 34602, "GCGCT": 27886, "GCGCC": 27406, "GCGCG": 19790, "GCGGA": 4362, "GCGGT": 2391, "GCGGC": 2552, "GCGGG": 4871, "GGAAA": 873384, "GGAAT": 350792, "GGAAC": 160101, "GGAAG": 379953, "GGATA": 350752, "GGATT": 264195, "GGATC": 175803, "GGATG": 22368, "GGACA": 153675, "GGACT": 101356, "GGACC": 84511, "GGACG": 9709, "GGAGA": 522957, "GGAGT": 59179, "GGAGC": 44920, "GGAGG": 314000, "GGTAA": 74428, "GGTAT": 44501, "GGTAC": 34124, "GGTAG": 255042, "GGTTA": 53769, "GGTTT": 44987, "GGTTC": 28252, "GGTTG": 17125, "GGTCA": 49312, "GGTCT": 25160, "GGTCC": 20496, "GGTCG": 278815, "GGTGA": 16713, "GGTGT": 11994, "GGTGC": 7607, "GGTGG": 282672, "GGCAA": 57636, "GGCAT": 36473, "GGCAC": 25973, "GGCAG": 15504, "GGCTA": 46947, "GGCTT": 30827, "GGCTC": 25469, "GGCTG": 15401, "GGCCA": 41643, "GGCCT": 24744, "GGCCC": 23134, "GGCCG": 5560, "GGCGA": 6008, "GGCGT": 4407, "GGCGC": 3869, "GGCGG": 5754, "GGGAA": 638632, "GGGAT": 199755, "GGGAC": 82864, "GGGAG": 297509, "GGGTA": 42220, "GGGTT": 36107, "GGGTC": 24211, "GGGTG": 22589, "GGGCA": 33259, "GGGCT": 28139, "GGGCC": 22356, "GGGCG": 6243, "GGGGA": 308863, "GGGGT": 38228, "GGGGC": 22542, "GGGGG": 1189550 }, "overrepresented_sequences": {} }, "command": "fastp -i /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/RP4 (paired)_R1.fastq.gz -I /mmfs1/gscratch/scrubbed/sr320/chil-wgbs/RP4 (paired)_R2.fastq.gz -o output/13-wgbs-hapa/trimmed/Rio4_R1.fq.gz -O output/13-wgbs-hapa/trimmed/Rio4_R2.fq.gz --detect_adapter_for_pe --trim_poly_g --trim_front1 10 --trim_front2 10 --length_required 50 --thread 16 --json output/13-wgbs-hapa/fastp/Rio4.fastp.json --html output/13-wgbs-hapa/fastp/Rio4.fastp.html " } }, "multiqc_general_stats": { "Pum1_pe": { "bismark-percent_cpg_meth": 19.8, "bismark-total_c": 827.325367, "bismark-C_coverage": 6.341, "bismark-dup_reads_percent": 7.01, "bismark-dedup_reads": 23.447231 }, "Qui1_pe": { "bismark-percent_cpg_meth": 19.2, "bismark-total_c": 799.475338, "bismark-C_coverage": 6.749, "bismark-dup_reads_percent": 7.01, "bismark-dedup_reads": 24.188775 }, "Qui2_pe": { "bismark-percent_cpg_meth": 22.0, "bismark-total_c": 763.050358, "bismark-C_coverage": 6.17, "bismark-dup_reads_percent": 6.4, "bismark-dedup_reads": 22.918882999999997 }, "Qui3_pe": { "bismark-percent_cpg_meth": 20.9, "bismark-total_c": 866.461299, "bismark-C_coverage": 7.072, "bismark-dup_reads_percent": 7.12, "bismark-dedup_reads": 25.944767 }, "Qui4_pe": { "bismark-percent_cpg_meth": 19.6, "bismark-total_c": 716.264236, "bismark-C_coverage": 6.043, "bismark-dup_reads_percent": 5.3, "bismark-dedup_reads": 21.93733 }, "Rio1_pe": { "bismark-percent_cpg_meth": 20.6, "bismark-total_c": 920.971231, "bismark-C_coverage": 7.351, "bismark-dup_reads_percent": 6.4, "bismark-dedup_reads": 26.616533999999998 }, "Rio2_pe": { "bismark-percent_cpg_meth": 20.4, "bismark-total_c": 776.530372, "bismark-C_coverage": 6.234, "bismark-dup_reads_percent": 6.18, "bismark-dedup_reads": 22.671502 }, "Rio3_pe": { "bismark-percent_cpg_meth": 21.5, "bismark-total_c": 673.982762, "bismark-C_coverage": 5.681, "bismark-dup_reads_percent": 4.81, "bismark-dedup_reads": 21.168215999999997 }, "Rio4_pe": { "bismark-percent_cpg_meth": 20.8, "bismark-total_c": 873.196166, "bismark-C_coverage": 7.143, "bismark-dup_reads_percent": 4.99, "bismark-dedup_reads": 25.909769999999998 }, "Pum1_PE_report": { "bismark-aligned_reads": 25.215539, "bismark-percent_aligned": 65.70607900482239 }, "Qui1_PE_report": { "bismark-aligned_reads": 26.013001, "bismark-percent_aligned": 67.18127197053228 }, "Qui2_PE_report": { "bismark-aligned_reads": 24.485685, "bismark-percent_aligned": 66.85013514488628 }, "Qui3_PE_report": { "bismark-aligned_reads": 27.932617999999998, "bismark-percent_aligned": 67.39467161507424 }, "Qui4_PE_report": { "bismark-aligned_reads": 23.165101999999997, "bismark-percent_aligned": 67.25461037542017 }, "Rio1_PE_report": { "bismark-aligned_reads": 28.437067, "bismark-percent_aligned": 68.49032618871512 }, "Rio2_PE_report": { "bismark-aligned_reads": 24.164697, "bismark-percent_aligned": 67.5339864541865 }, "Rio3_PE_report": { "bismark-aligned_reads": 22.237509, "bismark-percent_aligned": 67.85273038818886 }, "Rio4_PE_report": { "bismark-aligned_reads": 27.272038, "bismark-percent_aligned": 68.22625517859746 }, "PUM1 (paired)_R1": { "fastp-pct_duplication": 5.8064, "fastp-after_filtering_q30_rate": 96.3833, "fastp-after_filtering_q30_bases": 10244.127143, "fastp-filtering_result_passed_filter_reads": 76.75253, "fastp-after_filtering_gc_content": 18.7236, "fastp-pct_surviving": 98.36097191943847, "fastp-pct_adapter": 9.590254129204867 }, "Qui1 (paired)_R1": { "fastp-pct_duplication": 5.90184, "fastp-after_filtering_q30_rate": 96.6226, "fastp-after_filtering_q30_bases": 10318.661775999999, "fastp-filtering_result_passed_filter_reads": 77.441228, "fastp-after_filtering_gc_content": 18.9486, "fastp-pct_surviving": 98.4630171829735, "fastp-pct_adapter": 14.242979783386323 }, "Qui2 (paired)_R1": { "fastp-pct_duplication": 5.18274, "fastp-after_filtering_q30_rate": 96.0052, "fastp-after_filtering_q30_bases": 9728.707541, "fastp-filtering_result_passed_filter_reads": 73.255454, "fastp-after_filtering_gc_content": 18.7922, "fastp-pct_surviving": 98.3323669513802, "fastp-pct_adapter": 10.875088344885977 }, "Qui3 (paired)_R1": { "fastp-pct_duplication": 6.0187100000000004, "fastp-after_filtering_q30_rate": 96.4285, "fastp-after_filtering_q30_bases": 11038.498744999999, "fastp-filtering_result_passed_filter_reads": 82.89265999999999, "fastp-after_filtering_gc_content": 18.7414, "fastp-pct_surviving": 98.35887275354665, "fastp-pct_adapter": 12.193226633736032 }, "Qui4 (paired)_R1": { "fastp-pct_duplication": 4.51384, "fastp-after_filtering_q30_rate": 96.2209, "fastp-after_filtering_q30_bases": 9142.305416, "fastp-filtering_result_passed_filter_reads": 68.887774, "fastp-after_filtering_gc_content": 18.961100000000002, "fastp-pct_surviving": 98.27563135252866, "fastp-pct_adapter": 14.190562392733582 }, "RP1 (paired)_R1": { "fastp-pct_duplication": 5.31965, "fastp-after_filtering_q30_rate": 96.35719999999999, "fastp-after_filtering_q30_bases": 11108.538555, "fastp-filtering_result_passed_filter_reads": 83.03966, "fastp-after_filtering_gc_content": 18.7954, "fastp-pct_surviving": 98.46360106862852, "fastp-pct_adapter": 9.668679060325186 }, "RP2 (paired)_R1": { "fastp-pct_duplication": 5.11724, "fastp-after_filtering_q30_rate": 96.3112, "fastp-after_filtering_q30_bases": 9532.551151, "fastp-filtering_result_passed_filter_reads": 71.56307, "fastp-after_filtering_gc_content": 18.8357, "fastp-pct_surviving": 98.20980645154206, "fastp-pct_adapter": 11.002068959649248 }, "RP3 (paired)_R1": { "fastp-pct_duplication": 3.91718, "fastp-after_filtering_q30_rate": 96.271, "fastp-after_filtering_q30_bases": 8697.272676999999, "fastp-filtering_result_passed_filter_reads": 65.54639399999999, "fastp-after_filtering_gc_content": 18.9349, "fastp-pct_surviving": 98.181077136376, "fastp-pct_adapter": 14.293523197473004 }, "RP4 (paired)_R1": { "fastp-pct_duplication": 4.3347999999999995, "fastp-after_filtering_q30_rate": 96.33930000000001, "fastp-after_filtering_q30_bases": 10671.919288, "fastp-filtering_result_passed_filter_reads": 79.945874, "fastp-after_filtering_gc_content": 18.8384, "fastp-pct_surviving": 98.48383972325439, "fastp-pct_adapter": 11.374933444524595 } } } }