Bismark report for: output/13-wgbs-hapa/trimmed/Qui2_R1.fq.gz and output/13-wgbs-hapa/trimmed/Qui2_R2.fq.gz (version: v0.25.1) Bismark was run with Bowtie 2 against the bisulfite genome of /mmfs1/gscratch/scrubbed/sr320/github/project-ostrea-chil/data/13-wgbs-genome-hapa/ with the specified options: -q --score-min L,0,-0.6 -p 2 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 36627727 Number of paired-end alignments with a unique best hit: 24485685 Mapping efficiency: 66.9% Sequence pairs with no alignments under any condition: 6281750 Sequence pairs did not map uniquely: 5860292 Sequence pairs which were discarded because genomic sequence could not be extracted: 42 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 12253768 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 12231875 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1064712291 Total methylated C's in CpG context: 28584973 Total methylated C's in CHG context: 1344539 Total methylated C's in CHH context: 6040987 Total methylated C's in Unknown context: 145435 Total unmethylated C's in CpG context: 100975134 Total unmethylated C's in CHG context: 179781446 Total unmethylated C's in CHH context: 747985212 Total unmethylated C's in Unknown context: 1186487 C methylated in CpG context: 22.1% C methylated in CHG context: 0.7% C methylated in CHH context: 0.8% C methylated in Unknown context (CN or CHN): 10.9% Bismark completed in 0d 0h 54m 23s