Variant sites (alt >= 25% at informative depth >= 4 in any oyster): 10 seen in >= 2 oysters: 5 differ between Qui vs Rio (pooled alt-frequency difference >= 0.5): 4 [CpG-destroying: 2] 54865535 G>A Pum= Qui=1.0 Rio=0.318 54865537 C>T Pum=0.0 Qui=1.0 Rio=0.235 54865577 A>T Pum=0.0 Qui=0.929 Rio=0.357 54865578 T>A Pum=0.0 Qui=0.963 Rio=0.357 1 CpGs in target; 1 testable for SNPs (>= 8 pooled genotype reads at C and G in each of Qui, Rio) sample pop meth% calls CtoT_snp depth Pum1 Pum 66.7 3 0 7.0 Qui1 Qui 0.0 4 0 6.0 Qui2 Qui 0.0 7 0 6.5 Qui3 Qui 0.0 4 1 10.0 Qui4 Qui 0.0 5 0 7.0 Rio1 Rio 66.7 9 1 16.0 Rio2 Rio 100.0 5 0 10.0 Rio3 Rio 0.0 2 1 5.0 Rio4 Rio 66.7 6 0 8.0