Variant sites (alt >= 25% at informative depth >= 4 in any oyster): 21 seen in >= 2 oysters: 7 differ between Qui vs Rio (pooled alt-frequency difference >= 0.5): 4 [CpG-destroying: 3] 82587214 C>A Pum=0.714 Qui=0.955 Rio=0.4 82587315 C>T Pum=0.375 Qui=0.588 Rio=0.083 82587728 C>T Pum=0.833 Qui=0.9 Rio=0.25 82587955 C>T Pum=0.75 Qui=0.652 Rio=0.133 8 CpGs in target; 8 testable for SNPs (>= 8 pooled genotype reads at C and G in each of Qui, Rio) sample pop meth% calls CtoT_snp depth Pum1 Pum 34.1 44 0 15.3 Qui1 Qui 51.6 31 0 12.2 Qui2 Qui 26.2 65 0 15.5 Qui3 Qui 45.5 44 0 12.9 Qui4 Qui 49.0 51 0 11.7 Rio1 Rio 79.3 29 0 10.2 Rio2 Rio 87.0 23 0 6.9 Rio3 Rio 70.2 47 0 7.7 Rio4 Rio 66.0 47 0 10.0