Variant sites (alt >= 25% at informative depth >= 4 in any oyster): 29 seen in >= 2 oysters: 6 differ between Qui vs Rio (pooled alt-frequency difference >= 0.5): 4 [CpG-destroying: 2] 50562141 C>A Pum=1.0 Qui=0.4 Rio=1.0 50562175 G>A Pum=1.0 Qui=0.417 Rio=1.0 50562197 C>T Pum=1.0 Qui=0.455 Rio=1.0 50562431 C>A Pum=0.0 Qui=0.286 Rio=0.952 24 CpGs in target; 18 testable for SNPs (>= 8 pooled genotype reads at C and G in each of Qui, Rio) sample pop meth% calls CtoT_snp depth Pum1 Pum 6.3 79 0 8.9 Qui1 Qui 36.5 85 0 7.4 Qui2 Qui 52.2 69 0 11.4 Qui3 Qui 48.8 82 0 10.0 Qui4 Qui 36.4 66 0 7.9 Rio1 Rio 10.6 142 0 10.6 Rio2 Rio 2.0 51 0 7.4 Rio3 Rio 22.7 75 0 8.0 Rio4 Rio 31.1 45 0 8.5