Variant sites (alt >= 25% at informative depth >= 4 in any oyster): 48 seen in >= 2 oysters: 8 differ between Qui vs Rio (pooled alt-frequency difference >= 0.5): 3 [CpG-destroying: 1] 53657484 G>A Pum=0.0 Qui=0.2 Rio=1.0 53657741 C>A Pum=0.0 Qui=0.443 Rio=1.0 53657785 C>A Pum=0.0 Qui=0.491 Rio=1.0 27 CpGs in target; 19 testable for SNPs (>= 8 pooled genotype reads at C and G in each of Qui, Rio) sample pop meth% calls CtoT_snp depth Pum1 Pum 0.9 109 0 10.5 Qui1 Qui 19.4 160 3 10.8 Qui2 Qui 49.4 89 2 10.0 Qui3 Qui 24.9 245 0 15.1 Qui4 Qui 0.0 100 0 9.0 Rio1 Rio 76.7 90 3 10.6 Rio2 Rio 92.4 79 0 5.5 Rio3 Rio 76.4 123 0 8.7 Rio4 Rio 62.9 116 0 10.8